Effects of alloplastic graft material combined with a topical ozone application on calvarial bone defects in rats

Background: This study presents the evaluation of the damage in the bone tissue resulting from a calvarial defect in rats and the efficiency of exposure to an ozone application with an alloplastic bone graft on the calvarial bone damage. Materials and methods: Wistar male rats (n = 56) were divided into four groups: a control group (n = 14), defect and ozone group (n = 14), defect and graft group (n = 14), and defect, graft, and ozone group (n = 14). Under anaesthesia, a circular full-thickness bone defect was created in all groups, and the experimental groups were further divided into two sub-groups, with 7 rats in each group sacrificed at the end of the 4th and 8th weeks. Bone samples were dissected, fixed in 10% formalin solution, and decalcified with 5% ethylene-diamine-tetraacetic acid (EDTA). After the routine follow-up on tissues, immunostaining of osteopontin and osteonectin antibodies was applied to sections and observed under a light microscope. Results: The control group exhibited osteopontin and osteonectin expression in fibroblasts and inflammatory cells at the end of the 4th week with an acceleration at the 8th week. Ozone administration elucidated new trabecular bone formation by increasing osteoblastic activity. Lastly, our observations underscore that a combination of allograft and ozone application increased the osteoblast, osteocyte, and bone matrix development at the 4th and 8th weeks. Conclusions: Exposure to an ozone application with an alloplastic bone graft on calvarial bone damage may induce osteoblastic activity, matrix development, mature bone cell formation, and new bone formation in rats

Effects of nicotine administration in rats on MMP2 and VEGF levels in periodontal membrane

Background: Nicotine is associated with increased incidence of periodontal disease and poor response to therapy. This article aimed at identifying the expression of matrix metalloproteinases 2 (MMPs2) and vascular endothelial growth factor (VEGF) proteins on extracellular matrix, fibrous distribution and angiogenetic development in periodontitis caused by nicotine effects on periodontal membrane.Materials and methods: In this experimental study, rats were divided into nicotine and control groups. While the rats in the nicotine group (n = 6) were administered 2 mg/kg nicotine sulphate for 28 days, the animals in the control group (n = 6) were only administered 1.5 mL physiologic saline solution subcutaneously for 28 days.Results: Histological sections were prepared and immunohistochemically stained for MMP2 and VEGF. The sections stained with Trichrome-Masson were observed under light microscope. VEGF and MMP2 immunoreactivity of periodontal gingiva and dentin was assessed by immunohistochemical staining.Conclusions: Nicotine reduces MMP production, disrupts collagen synthesis and causes periodontitis. We observed that nicotine increases periodontitis by disrupting periodontal membrane and prevents tooth to anchor in dental alveoli by disrupting epithelial structure

Effects of formaldehyde on vascular endothelial growth factor, matrix metallopeptidase 2 and osteonectin levels in periodontal membrane and alveolar bone in rats

Background: The objective of this study was to investigate whether long term formaldehyde inhalation may affect periodontal membrane and alveolar bone loss leading to periodontitis. The negative effects of formaldehyde were described using vascular endothelial growth factor (VEGF), matrix metallopeptidase 2 (MMP-2) and osteonectin antibodies involved in the extracellular matrix and angiogenetic development. Materials and methods: Thirty adult Wistar albino rats were used in this study. Rats were divided into two groups: a control group (n = 15) and formaldehyde administered group (n = 15). Formaldehyde group was exposed to inhalation of 10 ppm formaldehyde 8 hours a day, 5 days a week for 5 weeks. Maxillary bone regions were dissected under anaesthesia. After fixation in 10% formaldehyde solution, tissues were passed through graded ethanol series to obtain paraffin blocks. Five-micrometre histological sections were cut with RM2265 rotary microtome stained with Masson trichrome and VEGF, MMP-2 and osteonectin antibodies for examination under Olympus BH-2 light microscopy. Results: The present study revealed that congestion in blood vessels, degeneration of collagen fibres and alveolar matrix around alveolar bone were observed to be more significant in formaldehyde group than the control group (p ≤ 0.001). Interestingly, VEGF expression in the formaldehyde group was the most significant finding between the two groups (p < 0.001). When compared inflammation, MMP-2 and osteonectin expressions were significant (p < 0.01) in the formaldehyde group. Conclusions: It was suggested that formaldehyde toxicity decreased the expression of MMP-2 and in osteoblasts as well as affecting the retention of MMP levels in tooth cavity, which is very low in collagen fibres. But, vice versa for the expression of VEGF in dilated vascular endothelial cells and osteocytes in alveolar bone. As a conclusion, formaldehyde disrupts the periodontal membrane and may cause collagen fibres degeneration by affecting the alveolar bone matrix

Evaluation of Metaverse Traffic Safety Implementations using fuzzy Einstein based logarithmic methodology of additive weights and TOPSIS method

As the Metaverse’s popularity grows, its effect on everyday problems is beginning to be discussed. The upcoming Metaverse world will influence the transportation system as cross-border lines blur due to rapid globalization. The purpose of this paper is to investigate the capabilities of the Metaverse and its alternatives to traffic safety, as well as to prioritize its advantages. The case study is based on a densely populated metropolis with an extensive education system. The city’s decision-makers will have to weigh the pros and cons of the Metaverse’s effect on traffic safety. To illustrate the complex forces that drive the decision-making process in traffic safety, we create a case study with four alternatives to Metaverse’s integration into the traffic system. Alternatives are evaluated using twelve criteria that reflect the decision problem’s rules and regulations, technology, socioeconomic, and traffic aspects. In this study, fuzzy Einstein based logarithmic methodology of additive weights (LMAW) is applied to calculate the weights of the criteria. We present a new framework that combines Einstein norms and the Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) method to rank the alternatives. The findings of this study show that public transportation is the most appropriate area for implementing the Metaverse into traffic safety because of its practical opportunities and broad usage area

Metastasis of breast carcinoma to the external auditory canal: Report of an unusual case and literature review

We present a case of metastasis of the external auditory canal (EAC) from a primary breast carcinoma in a 53-year-old female with a review of the literature. The patient had been diagnosed with a primary carcinoma 4 years previously. The metastasis had developed recently in her left EAC and presented as a bulky, fleshy, bleeding mass. The mass was causing hearing loss on the left due to complete obstruction in the left EAC. The mass was incompletely removed with a surgical operation and histopathologically metastasis was proven. Although there are few case reports in the literature of various cancers metastasizing to the EAC, metastasis in the EAC from the breast carcinoma is exceedingly rare and only one case has been reported in the literature so far

Investigation of antioxidant effects of rosmarinic acid on liver, lung and kidney in rats: a biochemical and histopathological study

Background: The aim of the study was to investigate the protective effects of rosmarinic acid in rats exposed to hepatic ischaemia/reperfusion (I/R) injury. Materials and methods: Thirty-two rats were randomly classified into four groups of 8 rats each: laparotomy without medication, rosmarinic acid (dose of 50 mg/kg via oral gavage) followed by laparotomy, laparotomy followed by hepatic I/R, and hepatic I/R with rosmarinic acid. Serum aspartate aminotransferase, alaninę aminotransferase, and malondialdehyde levels and total oxidant activity and total antioxidant capacity levels of the liver, lung, and kidney were assessed. The histopathologic assessment was also performed. Results: Rosmarinic acid significantly reduced liver function test parameters and decreased oxidative stress and abnormal histopathologic findings in the liver. The oxidative stress in the lung significantly increased in the I/R group but significantly decreased in the I/R + rosmarinic acid group due to the addition of rosmarinic acid. Rosmarinic acid led to no reduction in oxidative stress in kidney following hepatic I/R injury. There were no statistically significant differences among the groups regarding histopathologic changes in kidney and lung sections. Conclusions: Rosmarinic acid has antioxidant properties and is an effective hepatoprotective agent. However, although rosmarinic acid provides useful effects in the lung by increasing antioxidant capacity and reducing oxidative stress after I/R injury, it does not ameliorate histopathologic changes. These findings suggest that rosmarinic acid is likely to provide favourable outcomes in the treatment of hepatic I/R injury

STATUS OF WILD BOAR (Sus scrofa L.) ON THE ISLAND KRK

Divlja svinja u Republici Hrvatskoj autohtona je vrsta. Zakonski spada u krupnu, lovostajem zaštićenu vrstu divljači. Krajem prošlog stoljeća tjerana nagonom za širenjem svoje vrste plivanjem dolazi na otok. Krk je danas veoma kvalitetno stanište za život krupne divljači. Obilje hrane, vode, gusta vegetacija, raznolikost staništa, mir u lovištu, kvalitetno tlo, zapuštanje poljoprivrednih površina koje se pretvaraju u šikaru, rezultira stalnim prirastom postojeće divljači kao i zauzimanje prostora od nove pristigle divljači. Nekada je otok izgledao bitno drugačije nego kako izgleda danas. Obrađivao se svaki komadić zemlje, a danas se većina prepušta sukcesiji. Nova situacija u staništu uvjetuje i pojavu novih životinjskih vrsta. Upravo ovakva situacija pogoduje krupnoj, a ne odgovara sitnoj divljači. Divlje svinje nalaze se i u nelovnim površinama, gdje dolaze zbog hrane i mira, budući se tu zbog zakonskih propisa ne vrši lov. Nelovne površine služe kao rezervat iz kojeg nastanjuju najkvalitetnija staništa. «Crna divljač» na otoku Krku smatra se nepoželjnom vrstom divljači, te se sukladno naredbama ministarstva lovi cijele godine, bez obzira na spol i dob. Zbog odstrjela krmača vodilja, kvalitetnog staništa i velikog odstrjela bucanje i prašenje traje tijekom cijele lovne godine. Divljač pokušava opstati koristeći svoje prirodne mehanizme za očuvanje svoje vrste. U devet lovnih godina s otoka je izlučeno 3.602 grla divljih svinja. Kvalitetu staništa potvrđuje i snaga trofeja odstreljenih veprova, koja zbog nepravilnog odstrjela opada. Želja za potpunim izlučenjem divlje svinje s otoka Krka borba je sa prirodom, a za sada priroda uvijek pobjeđuje. Da li će tako biti i ovoga puta pokazati će budućnostThe wild boar is a native species in the Republic of Croatia. This large game animal is protected by law in closed season. At the end of the last century, driven by instinct to expand its species, it swam from the mainland to the island of Krk. Today Krk provides an excellent habitat for large game animals. Due to an abundance of food, water, dense vegetation, habitat diversity, peaceful hunting ground, fertile ground, abandoned farming areas which have been turning into thick underbrush, the existing game is on a constant increase while new arrivals crowd the existing habitat. The island has changed much and the landscape is not what it was once. Each parcel of land was once cultivated, while today it is left to succession. The new situation in the habitat has caused the emergence of new animal species. It is exactly this type of situation that suits the large and not the small game animals. Wild boars are found in areas not designated for hunting where they come to feed in a peaceful environment since by law hunting is prohibited there. Areas not designated for hunting serve as a game refuge and as such are considered to be habitats of the highest quality. The “black game” on the island of Krk is considered an undesirable type of game animal and pursuant to regulations may be hunted throughout the year, regardless of its sex or age. Due to the eradication of dominant female wild boars, excellent habitat and the large number of killings, mating lasts throughout the entire hunting season. The wild boar tries to survive using its natural mechanisms to preserve its species. In the nine years of hunting 3,602 head of wild boars were eradicated. The quality of the habitat is measured by the number of male boars that were killed as trophies, this being on the decline due to irregular killings. The desire to completely eradicate the wild boar from the island of Krk is a battle against nature and nature is winning, for now. Will this be the case now, only the future will tell

IL-6 mediated JAK/STAT3 signaling pathway in cancer patients with cachexia

CONCLUSION: STAT3 may be considered as a therapeutic target for cachectic patients with gastric, lung and breast cancer. Furthermore, IL-6 mediates STAT3 activation in cachectic gastric and breast cancer patients (Tab. 5, Fig. 2, Ref. 62)

Site selection of the Colombian antarctic research station based on fuzzy-topsis algorithm

By 2025 the Republic of Colombia aims to be an advisory member of the Antarctic Treaty System (ATS) and the installation of a scientific station is necessary to upscale the scientific capabilities. The aim of this paper is showing the results of the implementation of a Fuzzy TOPSIS algorithm for site selection of the Colombian Antarctic Scientific Station. A three-phase methodology was AQ1 proposed, and the obtained results allowed to identify the optimum location for the station, considering key success factors and regulatory constraints

Tissue Harvester with Functional Valve (THFV): Shidham's device for reproducibly higher specimen yield by fine needle aspiration biopsy with easy to perform steps

BACKGROUND: Fine needle aspiration biopsy (FNAB) cytology has been a highly effective methodology for tissue diagnosis and for various ancillary studies including molecular tests. In addition to other benefits, FNAB predominantly retrieves the diagnostic loosely cohesive cells in the lesion as compared to the adjacent supporting stroma with relatively higher cohesiveness. However, FNAB procedure performed with currently available resources is highly skill dependent with inter-performer variability, which compromises its full potential as a diagnostic tool. In this study we report a device overcoming these limitations. METHODS: 'Tissue Harvester with Functional Valve' (THFV) was evaluated as part of a phase 1 National Institute of Health (NIH) research grant under Small Business Technology Transfer (STTR) Program. Working prototypes of the device were prepared. Each of the four cytopathologists with previous cytopathology fellowship training and experience in performing FNAB evaluated 5 THFV and 5 hypodermic needles resulting in 40 specimens (20 with THFV, 20 with hypodermic needles). A piece of fresh cattle liver stuffed in latex glove was used as the specimen. Based on these results a finished design was finalized. RESULTS: The smears and cell blocks prepared from the specimens obtained by THFV were superior in terms of cellularity to specimens obtained with hypodermic needles. The tissuecrit of specimens obtained with THFV ranged from 70 to 100 μl (mean 87, SD 10), compared to 17 to 30 μl (mean 24, SD 4) with conventional hypodermic needles (p < .0001, Student t-test). The technical ease [on a scale of 1 (easy) to 5 (difficult)] with THFV ranged from 1 to 2 as compared to 2 to 3 with hypodermic needles. CONCLUSION: The specimen yield with the new THFV was significantly higher when compared to hypodermic needles. Also, the FNAB procedure with THFV was relatively easier in comparison with hypodermic needles. The final version of Shidham's THFV device would improve the FNAB specimen yield by eliminating the skill factor. The increased specimen yield by this device would also facilitate wider application of FNAB specimens for various ancillary tests, including molecular tests