The GEMPAK Barnes objective analysis scheme

GEMPAK, an interactive computer software system developed for the purpose of assimilating, analyzing, and displaying various conventional and satellite meteorological data types is discussed. The objective map analysis scheme possesses certain characteristics that allowed it to be adapted to meet the analysis needs GEMPAK. Those characteristics and the specific adaptation of the scheme to GEMPAK are described. A step-by-step guide for using the GEMPAK Barnes scheme on an interactive computer (in real time) to analyze various types of meteorological datasets is also presented

Atomic and Nuclear Effects in the Slow-Neutron Total Cross Section of Terbium

The total cross section of terbium has been measured for neutron energies En from 0.003 to 1.78 eV. For neutron energies above the Be cutoff a new method employing two crystal monochromators in series was used. For En ≲ 0.005 eV a single-crystal monochromator in conjunction with a Be filter was employed. Using earlier measurements of the radiative-capture cross section, the experimental results have been analyzed to include the contributions due to paramagnetic scattering, coherent scattering, and phonon effects. The analysis shows that the experiment and the calculations are consistent everywhere except 0.015 ≲ En ≲ 0.10 eV. In this energy range inelastic coherent scattering is not accurately accounted for with the use of Placzek\u27s incoherent approximations. In addition crystalline-field effects give rise to further complications. The comparison between the experiment and the calculations for 0.015 ≲ En ≲ 0.10 eV suggests that studies of coherent inelastic scattering and crystalline-field effects in terbium metal are needed. The experimental results for En ≳ 0.12 eV yield the potential scattering cross section as 7.5 ± 0.5 b. This corresponds to a spin-independent nuclear radius of 7.73 ± 0.27 fm and to a radius parameter of 1.43 ± 0.05 fm. The results suggest that the incoherent scattering cross section, if present, is very small (≲ 1.0 b)

Comparaison de techniques d’identification des Erwinia et des Pseudomonas responsables de la pourriture molle

Trois méthodes, soit la caractérisation physiologique, l'utilisation de systèmes miniaturisés d'identification (API 20E, API NFT et Biolog) et l'analyse du profil électrophorétique des protéines sécrétées, ont été expérimentées afin de déterminer une technique précise et rapide d'identification des Pseudomonas et des Erwinia responsables de la pourriture molle. L'analyse des patrons électrophorétiques des protéines sécrétées est une méthode très efficace pour identifier les différentes espèces pectinolytiques de Pseudomonas fluorescents. Le système Biolog reconnaît efficacement le P. marginalis et le P. viridiflava. Le système API NFT est efficace pour l'identification du P. marginalis, du P. viridiflava et du P. syringae. C'est le système API 20E qui s'est avéré le plus efficace pour l'identification des Erwinia. L'électrophorèse des protéines sécrétées et le système API NFT permettent une identification rapide et efficace des Pseudomonas, tandis que pour les Erwinia, seul le système API20E est performant.Three methods, namely physiological characterization, the use of miniaturized identification Systems (API 20E, API NFT, and Biolog) and the analysis of the electrophoretic profile of proteins secreted by the bacteria, were used in order to determine a precise and quick identification technique for the Pseudomonas and Erwinia species causing soft rots in several plant species. The analysis of the electrophoretic profile of the proteins secreted is a very efficient method of identification for the various pectinolytic species of fluorescent Pseudomonas. The Biolog System accurately recognizes P. marginalisand P. viridiflava. The API NFT System is efficient for the identification of P. marginalis, P. viridiflava and P. syringae. The API 20E System was found to be the most reliable System for identifying Erwinia species. The electrophoresis of proteins secreted and the API NFT system allow a quick and efficient identification of Pseudomonas species, while for Erwinia species, only the API 20E System is efficient

Exploring X-ray Binary Populations in Compact Group Galaxies with ChandraChandra

We obtain total galaxy X-ray luminosities, $L_X$, originating from individually detected point sources in a sample of 47 galaxies in 15 compact groups of galaxies (CGs). For the great majority of our galaxies, we find that the detected point sources most likely are local to their associated galaxy, and are thus extragalactic X-ray binaries (XRBs) or nuclear active galactic nuclei (AGNs). For spiral and irregular galaxies, we find that, after accounting for AGNs and nuclear sources, most CG galaxies are either within the $\pm1\sigma$ scatter of the Mineo et al. (2012) $L_X$ - star formation rate (SFR) correlation or have higher $L_X$ than predicted by this correlation for their SFR. We discuss how these "excesses" may be due to low metallicities and high interaction levels. For elliptical and S0 galaxies, after accounting for AGNs and nuclear sources, most CG galaxies are consistent with the Boroson et al. (2011) $L_X$ - stellar mass correlation for low-mass XRBs, with larger scatter, likely due to residual effects such as AGN activity or hot gas. Assuming non-nuclear sources are low- or high-mass XRBs, we use appropriate XRB luminosity functions to estimate the probability that stochastic effects can lead to such extreme $L_X$ values. We find that, although stochastic effects do not in general appear to be important, for some galaxies there is a significant probability that high $L_X$ values can be observed due to strong XRB variability.Comment: Accepted by Ap

Contributions of proteomics to understanding phagosome maturation

In metazoans macrophage cells use phagocytosis, the process of engulfing large particles, to control the spread of pathogens in the body, to clear dead or dying cells, and to aid in tissue remodelling, while the same process is also used by unicellular eukaryotes to ingest food. Phagocytosing cells essentially swallow the particles, trapping them in vacuoles called phagosomes that go through a series of maturation steps, culminating in the destruction of the internalized cargo. Because of their central role in innate immunity and their relatively simple structure (one membrane bilayer surrounding a single particle), phagosomes have been a popular subject for organelle proteomics studies. Qualitative proteomic technologies are now very sensitive so hundreds of different proteins have been identified in phagosomes from several species, revealing new properties of these intriguing compartments. More recently, quantitative proteomic approaches have also been applied, shedding new light on the dynamics and composition of maturing phagosomes. In this review we summarize the studies that have applied proteomic technologies to phagosomes and how they have changed our understanding of phagosome biology

The origin of the E+ transition in GaAsN alloys

Optical properties of GaAsN system with nitrogen concentrations in the range of 0.9-3.7% are studied by full-potential LAPW method in a supercell approach. The E+ transition is identified by calculating the imaginary part of the dielectric function. The evolution of the energy of this transition with nitrogen concentration is studied and the origin of this transition is identified by analyzing the contributions to the dielectric function from different band combinations. The L_1c-derived states are shown to play an important role in the formation of the E+ transition, which was also suggested by recent experiments. At the same time the nitrogen-induced modification of the first conduction band of the host compound are also found to contribute significantly to the E+ transition. Further, the study of several model supercells demonstrated the significant influence of the nitrogen potential on the optical properties of the GaAsN system.Comment: 5 pages, 3 figure

Photoacoustic imaging of intracardiac medical devices using internal illumination of carbon nanotube / PDMS composite coatings

Accurate localisation of medical devices is of crucial importance for a wide range of ultrasound-guided interventions. In this study, we investigated visualisation of medical devices by photoacoustic excitation of optically absorbing coatings. Photoacoustic excitation light was provided through optical fibres positioned within a cardiac needle and a steerable-tip catheter. Using a swine heart model, photoacoustic and B-mode ultrasound images were received with a clinical ultrasound scanner in conjunction with a transoesophageal imaging probe. In the photoacoustic images, prominent signals were obtained from the coatings. This study demonstrated that photoacoustic imaging could play a useful role with medical device imaging

A Chandra−SwiftChandra-Swift View of Point Sources in Hickson Compact Groups: High AGN fraction but a dearth of strong AGNs

We present $Chandra$ X-ray point source catalogs for 9 Hickson Compact Groups (HCGs, 37 galaxies) at distances $34 - 89$ Mpc. We perform detailed X-ray point source detection and photometry, and interpret the point source population by means of simulated hardness ratios. We thus estimate X-ray luminosities ($L_X$) for all sources, most of which are too weak for reliable spectral fitting. For all sources, we provide catalogs with counts, count rates, power-law indices ($\Gamma$), hardness ratios, and $L_X$, in the full ($0.5-8.0$ keV), soft ($0.5-2.0$ keV) and hard ($2.0-8.0$ keV) bands. We use optical emission-line ratios from the literature to re-classify 24 galaxies as star-forming, accreting onto a supermassive black hole (AGNs), transition objects, or low-ionization nuclear emission regions (LINERs). Two-thirds of our galaxies have nuclear X-ray sources with $Swift$/UVOT counterparts. Two nuclei have $L_{X,{\rm 0.5-8.0 keV}}$~$> 10^{42}$ erg s$^{-1}$, are strong multi-wavelength AGNs and follow the known $\alpha_{\rm OX}-\nu L_{\nu,\rm near UV}$ correlation for strong AGNs. Otherwise, most nuclei are X-ray faint, consistent with either a low-luminosity AGN or a nuclear X-ray binary population, and fall in the "non-AGN locus" in $\alpha_{\rm OX}-\nu L_{\nu,\rm near UV}$ space, which also hosts other, normal, galaxies. Our results suggest that HCG X-ray nuclei in high specific star formation rate spiral galaxies are likely dominated by star formation, while those with low specific star formation rates in earlier types likely harbor a weak AGN. The AGN fraction in HCG galaxies with $M_R \le -20$ and $L_{X,{\rm 0.5-8.0 keV}} \ge 10^{41}$ erg s$^{-1}$ is $0.08^{+0.35}_{-0.01}$, somewhat higher than the $\sim 5$ fraction in galaxy clusters.Comment: 77 pages (emulateapj), 28 tables, 11 figures. Accepted by ApJS on March 5, 201

Stellar Populations in Compact Galaxy Groups: a Multi-Wavelength Study of HCGs 16, 22, and 42, their Star Clusters and Dwarf Galaxies

We present a multi-wavelength analysis of three compact galaxy groups, HCGs 16, 22, and 42, which describe a sequence in terms of gas richness, from space- (Swift, HST, Spitzer) and ground-based (LCO, CTIO) imaging and spectroscopy. We study various signs of past interactions including a faint, dusty tidal feature about HCG 16A, which we tentatively age-date at <1 Gyr. This represents the possible detection of a tidal feature at the end of its phase of optical observability. Our HST images also resolve what were thought to be double nuclei in HCG 16C and D into multiple, distinct sources, likely to be star clusters. Beyond our phenomenological treatment, we focus primarily on contrasting the stellar populations across these three groups. The star clusters show a remarkable intermediate-age population in HCG 22, and identify the time at which star formation was quenched in HCG 42. We also search for dwarf galaxies at accordant redshifts. The inclusion of 33 members and 27 'associates' (possible members) radically changes group dynamical masses, which in turn may affect previous evolutionary classifications. The extended membership paints a picture of relative isolation in HCGs 16 and 22, but shows HCG 42 to be part of a larger structure, following a dichotomy expected from recent studies. We conclude that (a) star cluster populations provide an excellent metric of evolutionary state, as they can age-date the past epochs of star formation; and (b) the extended dwarf galaxy population must be considered in assessing the dynamical state of a compact group.Comment: 29 pages, 13 figures. Accepted for publication in The Astrophysical Journa

Multi-scale spectrally resolved quantitative fluorescence imaging system: Towards neurosurgical guidance in glioma resection

In glioma resection surgery, the detection of tumour is often guided by using intraoperative fluorescence imaging notably with 5-ALA-PpIX, providing fluorescent contrast between normal brain tissue and the gliomas tissue to achieve improved tumour delineation and prolonged patient survival compared with the conventional white-light guided resection. However, the commercially available fluorescence imaging system relies on surgeon’s eyes to visualise and distinguish the fluorescence signals, which unfortunately makes the resection subjective. In this study, we developed a novel multi-scale spectrally-resolved fluorescence imaging system and a computational model for quantification of PpIX concentration. The system consisted of a wide-field spectrally-resolved quantitative imaging device and a fluorescence endomicroscopic imaging system enabling optical biopsy. Ex vivo animal tissue experiments as well as human tumour sample studies demonstrated that the system was capable of specifically detecting the PpIX fluorescent signal and estimate the true concentration of PpIX in brain specimen