13 research outputs found
Metagenomic insights into the abundance and composition of resistance genes in aquatic environments:Influence of stratification and geography
A global survey was performed with 122 aquatic metagenomic DNA datasets (92 lake water and 30 seawater) obtained from the Sequence Read Archive (SRA). Antibiotic resistance genes (ARGs) and metal resistance genes (MRGs) were derived from the dataset sequences via bioinformatic analysis. The relative abundances of ARGs and MRGs in lake samples were in the ranges ND (not detected)-1.34x10(0) and 1.22x10(-3) -1.98x10(-1) copies per 16S rRNA, which were higher than those in seawater samples. Among ARGs, multidrug resistance genes and bacitracin resistance genes had high relative abundances in both lake and sea water samples. Multimetal resistance genes, mercury resistance genes and copper resistance genes had the greatest relative abundance for MRGs. No significant difference was found between epilimnion and hypolimnion in abundance or the Shannon diversity index for ARGs and MRGs. Principal coordinates analysis and permutational multivariate analysis of variance (PERMANOVA) test showed that stratification and geography had significant influence on the composition of ARGs and MRGs in lakes (p < 0.05, PERMANOVA). Coastal seawater samples had significantly greater relative abundance and a higher Shannon index for both ARGs and MRGs than deep ocean and Antarctic seawater samples (p < 0.05, Kruskal-Wallis one-way ANOVA), suggesting that human activity may exert more selective pressure on ARGs and MRGs in coastal areas than those in deep ocean and Antarctic seawater
Gastric mixed neuroendocrine non-neuroendocrine neoplasms
The uncommon tumour known as gastric mixed neuroendocrine-non-neuroendocrine neoplasms (G-MiNENs) is made up of parts of neuroendocrine carcinoma and adenocarcinoma. The biological and clinical features are different from those of gastric adenocarcinoma. Their pathophysiology, diagnostic standards, and clinical behaviour have all been the subject of lengthy debates, and their nomenclature has undergone multiple changes. Its emergence has created new challenges in the classification and diagnosis of gastric tumours. This review will update information on the topic, covering molecular aspects, diagnostic criteria, treatment, and prognostic factor discovery. It will also provide a historical context that will aid in understanding the evolution of the idea and nomenclature of mixed gastric tumours. Additionally, it will provide the reader a thorough understanding of this difficult topic of cancer that is applicable to real-world situations
Pharmacodynamics, metabolomics and pathological studies on mechanisms of traditional benefits of Angelica sinensis in blood circulation
Angelica sinensis is a rich source of medically important active molecules that need in-depth understanding on its action mechanisms. Therefore, through pharmacodynamics, metabolomics, and network pharmacology, the traditional benefits of A. sinensis in blood circulation was studied using 24 randomly selected Sprague-Dawley (SD) rats. Measurement of the blood rheological parameters for whole blood viscosity (WBV) and plasma viscosity (PV), and inspection of the heart and lung tissues pathological changes were undertaken using molecular and bioinformatic techniques. Multivariate statistical analysis and establishment of the model of the relationship between metabolite expression and sample categories to test the prediction of sample categories were performed. Screening was undertaken to find the potential metabolites for A. sinensis to treat blood stasis syndrome and find related metabolic pathways. Active ingredients of A. sinensis and targets and building of an âeffect component-targetâ network was undertaken, A. sinensis was confirmed to improve blood stasis syndrome in rats and improve heart and lung pathology to varying degrees. Compared with the blood stasis model group, A. sinensis significantly reduced WBV and PV in hemorheology (p<0.05, p<0.01) and regulated blood stasis-induced changes in 22 metabolites including alpha-D-glucose, L-isoleucine, creatine and acetylcarnitine, which are involved in the metabolism of linoleic acid, linolenic acid, phenylalanine, ascorbic acid and uronic acid. Using the network pharmacology to build a "component-target-pathway" network of A. sinensis, 62 active ingredients, 169 active proteins and 18 metabolic pathways were obtained, among which linoleic acid metabolism, ascorbic acid and uronic acid metabolism were consistent with the metabolic pathways obtained by metabolomics
Denitrification and anammox separately dominate nitrogen loss in the riparian rhizosphere and bulk soils along a latitudinal gradient
Raw data of "Denitrification and anammox separately dominate nitrogen loss in the riparian rhizosphere and bulk soils along a latitudinal gradient"</p
Pharmacodynamics, metabolomics and pathological studies on mechanisms of traditional benefits of Angelica sinensis in blood circulation
122-132Angelica sinensis is a rich source of medically important active molecules that need in-depth understanding on its action
mechanisms. Therefore, through pharmacodynamics, metabolomics, and network pharmacology, the traditional benefits of
A. sinensis in blood circulation was studied using 24 randomly selected Sprague-Dawley (SD) rats. Measurement of the
blood rheological parameters for whole blood viscosity (WBV) and plasma viscosity (PV), and inspection of the heart and
lung tissues pathological changes were undertaken using molecular and bioinformatic techniques. Multivariate statistical
analysis and establishment of the model of the relationship between metabolite expression and sample categories to test the
prediction of sample categories were performed. Screening was undertaken to find the potential metabolites for A. sinensis
to treat blood stasis syndrome and find related metabolic pathways. Active ingredients of A. sinensis and targets and
building of an âeffect component-targetâ network was undertaken, A. sinensis was confirmed to improve blood stasis
syndrome in rats and improve heart and lung pathology to varying degrees. Compared with the blood stasis model group, A.
sinensis significantly reduced WBV and PV in hemorheology (p<0.05, p<0.01) and regulated blood stasis-induced changes
in 22 metabolites including alpha-D-glucose, L-isoleucine, creatine and acetylcarnitine, which are involved in the
metabolism of linoleic acid, linolenic acid, phenylalanine, ascorbic acid and uronic acid. Using the network pharmacology to
build a "component-target-pathway" network of A. sinensis, 62 active ingredients, 169 active proteins and 18 metabolic
pathways were obtained, among which linoleic acid metabolism, ascorbic acid and uronic acid metabolism were consistent
with the metabolic pathways obtained by metabolomics
Bone Marrow Mesenchymal Stem CellâDerived DermcidinâContaining Migrasomes enhance LC3âAssociated Phagocytosis of Pulmonary Macrophages and Protect against PostâStroke Pneumonia
Abstract Pneumonia is one of the leading causes of death in patients with acute ischemic stroke (AIS). Antibiotics fail to improve prognosis of patients with postâstroke pneumonia, albeit suppressing infection, due to adverse impacts on the immune system. The current study reports that bone marrow mesenchymal stem cells (BMâMSC) downregulate bacterial load in the lungs of stroke mice models. RNAâsequencing of the lung from BMâMSCâtreated stroke models indicates that BMâMSC modulates pulmonary macrophage activities after cerebral ischemia. Mechanistically, BMâMSC promotes the bacterial phagocytosis of pulmonary macrophages through releasing migrasomes, which are migrationâdependent extracellular vesicles. With liquid chromatographyâtandem mass spectrometry (LCâMS/MS), the result shows that BMâMSC are found to load the antibacterial peptide dermcidin (DCD) in migrasomes upon bacterial stimulation. Besides the antibiotic effect, DCD enhances LC3âassociated phagocytosis (LAP) of macrophages, facilitating their bacterial clearance. The data demonstrate that BMâMSC is a promising therapeutic candidate against postâstroke pneumonia, with dual functions of antiâinfection and immunol modulation, which is more than a match for antibiotics treatment
Analysing Enterprise Models from a Fractal Organisation Perspective - Potentials and Limitations
Part 6: Enterprise ModelingInternational audienceThe paper focuses on the use of the fractal paradigm in enterprise modeling. It investigates whether the properties of fractal organizations can be applied in business analysis and whether this results in useful outcomes and new insights. Based on an adaptation and operationalization of properties of fractal organizations, two real-world cases are analyzed using the adapted properties. The contributions of this paper are (1) to adapt fractal organisation properties for use in analysis of enterprise models, (2) to present practical examples from two cases showing the application of the fractal organisation properties, and (3) to identify potentials and limitations of using fractal organisation perspective in enterprise model analysis