The Impact of Financial Access on Firm Growth: Evidence from Ethiopian Grain Traders and Millers

Although both formal and informal financial institutions exist in developing economies, firms are often constrained by lack of access to financial services. Grain traders and millers in Ethiopia need a lot of finance to pay their suppliers (e.g. farmers) but it is not clear whether or not which sources of credit matter most for their growth and expansion. Using firm survey data collected for the purpose, we assessed access to and the impact of different sources of finance on growth of traders and millers in Ethiopia. Descriptive and econometric methods (e.g. ordered probit) were employed to address the issue. The results indicate that both formal and informal sources of credits are accessed by a small number of firms on a sporadic basis. With credit from commercial banks is mainly channeled to large businesses while microfinance institutions (MFIs) are designed to assist small and micro enterprises as part of a poverty alleviation strategy, medium firms such as most grain traders and millers have limited access to finance. Bank or MFI credit was found to have no impact on growth and expansion in the econometric analysis. Access to informal credit is also limited and largely used to meet short-term emergency cash requirements. Without improved and regular access to finance, grain traders and millers cannot make the necessary investment to provide effective marketing services for the transformation of agriculture.Key words: finance, financial access, Growth, Grain, Grain traders and millers, Ethiopi

Evaluating the impact of the graduate fellowship programme of the International Livestock Research Institute. A tools and process report

Post graduate research projects are an effective method for building new research capacity in sub-Saharan Africa. The International Livestock Research Institute (ILRI) offers a graduate fellowship programme for scientists from developing and developed countries. This is done in partnership with universities in African and Asian countries, Europe, Australia and North America. To date such programmes have not been adequately assessed to find the impact on national research capacity, the graduate fellows and the partner institutions. To assess the value of this training, ILRI conducted an impact study of its graduate fellows in Kenya and Ethiopia between 1978 and 1997. This report presents the tools and methods used in this study. Others may use these freely, but appropriate acknowledgement of the source will be appreciated. Future users may modify these instruments for their own impact studies; and indeed are encouraged to do so. We recognise that many improvements could be made and request feedback from others on how they have accomplished this. This report also presents part of the results to enable those interested in future impact studies in Ethiopia or Kenya to use the data as a benchmark

Development of a D-xylose fermenting and inhibitor tolerant industrial Saccharomyces cerevisiae strain with high performance in lignocellulose hydrolysates using metabolic and evolutionary engineering

Background: The production of bioethanol from lignocellulose hydrolysates requires a robust, D-xylose-fermenting and inhibitor-tolerant microorganism as catalyst. The purpose of the present work was to develop such a strain from a prime industrial yeast strain, Ethanol Red, used for bioethanol production. Results: An expression cassette containing 13 genes including Clostridium phytofermentans XylA, encoding D-xylose isomerase (XI), and enzymes of the pentose phosphate pathway was inserted in two copies in the genome of Ethanol Red. Subsequent EMS mutagenesis, genome shuffling and selection in D-xylose-enriched lignocellulose hydrolysate, followed by multiple rounds of evolutionary engineering in complex medium with D-xylose, gradually established efficient D-xylose fermentation. The best-performing strain, GS1.11-26, showed a maximum specific D-xylose consumption rate of 1.1 g/g DW/h in synthetic medium, with complete attenuation of 35 g/L D-xylose in about 17 h. In separate hydrolysis and fermentation of lignocellulose hydrolysates of Arundo donax (giant reed), spruce and a wheat straw/hay mixture, the maximum specific D-xylose consumption rate was 0.36, 0.23 and 1.1 g/g DW inoculum/h, and the final ethanol titer was 4.2, 3.9 and 5.8% (v/v), respectively. In simultaneous saccharification and fermentation of Arundo hydrolysate, GS1.11-26 produced 32% more ethanol than the parent strain Ethanol Red, due to efficient D-xylose utilization. The high D-xylose fermentation capacity was stable after extended growth in glucose. Cell extracts of strain GS1.11-26 displayed 17-fold higher XI activity compared to the parent strain, but overexpression of XI alone was not enough to establish D-xylose fermentation. The high D-xylose consumption rate was due to synergistic interaction between the high XI activity and one or more mutations in the genome. The GS1.11-26 had a partial respiratory defect causing a reduced aerobic growth rate. Conclusions: An industrial yeast strain for bioethanol production with lignocellulose hydrolysates has been developed in the genetic background of a strain widely used for commercial bioethanol production. The strain uses glucose and D-xylose with high consumption rates and partial cofermentation in various lignocellulose hydrolysates with very high ethanol yield. The GS1.11-26 strain shows highly promising potential for further development of an all-round robust yeast strain for efficient fermentation of various lignocellulose hydrolysates

Incidence and Predictors of Pre-Eclampsia Among Pregnant Women Attending Antenatal Care at Debre Markos Referral Hospital, North West Ethiopia: Prospective Cohort Study.

BackgroundPre-eclampsia is a pregnancy-induced hypertension that occurs after 20 weeks of gestation. It is the leading cause of maternal and perinatal morbidity and mortality globally, but it is higher in developing countries. In Ethiopia, conducting research on the incidence and predictors of pre-eclampsia is crucial due to the paucity of information.MethodsA prospective cohort study was undertaken using 242 pregnant women between November 1, 2018 and March 30, 2019 at Debre Markos Referral Hospital. All eligible women who fulfilled the inclusion criteria were included in this study. Data were entered into the epic-data Version 4.2 and analyzed using the STATA Version 14.0 software. The Cox-proportional hazard regression model was fitted and Cox-Snell residual test was used to assess the goodness of fit. Pre-eclampsia free survival time was estimated using the Kaplan-Meier survival curve. Both bivariable and multivariable Cox-proportional hazard regression models were fitted to identify predictors of pre-eclampsia.ResultsThe overall incidence rate of pre-eclampsia was 3.35 per 100 person-years. Having a pre-existing history of diabetes mellitus [AHR=2.7 (95% CI=1.43-8.81)], having a history of multiple pregnancy [AHR=3.4 (95% CI=2.8-6.9)] and being ≥35 years old age [AHR=2.5 (95% CI=1.42-3.54)] were the significant predictors of pre-eclampsia.ConclusionThe incidence of pre-eclampsia was high in this study. Having (pre-existing diabetes and multiple pregnancy) and being ≥35 years old age were the significant predictors of pre-eclampsia. Inspiring pregnant women's health-seeking behavior should provide a chance to diagnose pre-eclampsia early to prevent the medical complication of pre-eclampsia

Food-fodder performance of food and malt barley cultivars in Ethiopian highlands

In Ethiopia, barley selection has focused on grain yield traits. Limited information is available on straw yield and its nutritive value. The aim of this study was to screen cultivars for grain and straw yield and nutritive value using forty cultivars of food and malt barley types at two locations in Ethiopia (Bekoji and Kofele) in 2018. Food-fodder quality traits investigated were crude protein (CP), neutral detergent fibre (NDF) and metabolizable energy (ME) of grain and straw. Location, cultivar and their interaction affected the performance in malt as well as food barley types. Wide cultivars differences were observed within food and malt types respectively: Grain CP: 10.2-12.2% and 11.4.1-13.3%, grain NDF: 40-2-52.7% and 38-42.9%, grain ME: 9.9-12.3 MJ/kg and 12.1-14.5 MJ/kg, straw CP: 4.1-5.7% and 4.9-6.2%, straw NDF: 73.5-76.7% and 72.9-76.1%, straw ME: 5-5.6 MJ/kg and 5.3-5.8 MJ/kg. Across locations, IBON174/03 produced most grain (6.95 t/ha), traveller produced most straw (9.1t/ha) and HB1963 was relatively high in both straw 8.4 t/ha and grain yield 6.4 t/ha, making it an interesting food-feed cultivar. Therefore IBON174/03, traveller and HB1963 are promising barley cultivars for the study area

Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection.

BACKGROUND:Most genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assays designed to detect GM plants often target the P35S and/or TNOS DNA sequences. However, because the P35S promoter is derived from CaMV, these detection assays can yield false-positives from non-GM plants infected by this naturally-occurring virus. RESULTS:Here we report the development of an assay designed to distinguish CaMV-infected plants from GM plants in a single multiplexed quantitative PCR (qPCR) reaction. Following initial testing and optimization via PCR and singleplex-to-multiplex qPCR on both plasmid and plant DNA, TaqMan qPCR probes with different fluorescence wavelengths were designed to target actin (a positive-control plant gene), P35S, P3 (a CaMV-specific gene), and TNOS. We tested the specificity of our quadruplex qPCR assay using different DNA extracts from organic watercress and both organic and GM canola, all with and without CaMV infection, and by using commercial and industrial samples. The limit of detection (LOD) of each target was determined to be 1% for actin, 0.001% for P35S, and 0.01% for both P3 and TNOS. CONCLUSIONS:This assay was able to distinguish CaMV-infected plants from GM plants in a single multiplexed qPCR reaction for all samples tested in this study, suggesting that this protocol is broadly applicable and readily transferrable to any interested parties with a qPCR platform

Effect of barley variety on feed intake, digestibility, body weight gain and carcass characteristics in fattening lambs

Twenty lambs (18 ± 0.22 kg initial weight) were blocked by weight and individually assigned into pens to evaluate the effects of barley straw variety on digestibility, growth performance and carcass characteristics. The following four treatments were tested: (1) a local barley straw (as control), (2) HB1963 (high grain and straw yields), (3) Traveller (high straw yielder), and (4) IBON174/03 (high grain yielder). A concentrate (50:50 wheat bran and noug seed cake) was offered constantly (300 DM g), whereas the straw was offered ad libitum. The digestibility trial lasted 22 days (15 days to adapt to dietary treatments and 7 days for sampling). The growth performance trial lasted 90 days. At the end, all of the lambs were slaughtered, and their carcasses were evaluated. The IBON174/03 variety had a higher (p < 0.05) intake of organic matter and crude protein, a higher dry matter and organic matter digestibility than the control, and a faster growth than the control. The feed-to-gain ratio was similar among treatments. The slaughter and empty body weights of lambs in the IBON174/03 group were higher than the control variety (p < 0.05). The present study showed that the feeding value of barley straw can differ substantially between varieties and therefore must be considered in the choice of a barley variety

Effectiveness of Agronomic Biofortification Strategy in Fighting against Hidden Hunger

Micronutrient deficiencies (MNDs), also known as hidden hunger, affect more than a quarter of the global population. Agronomic biofortification helps to increase the concentration of a target mineral in food crops and improve human mineral dietary intake. It is a means of providing nutrient-dense foods to a larger population, especially among rural resource-poor settings, providing that they have access to mineral fertilizers. However, the feasibility of agronomic biofortification in combating hidden hunger depends on several factors in addition to fertilizer access, including crop type, genotype, climate, soils, and soil mineral interactions. Consideration of its effectiveness in increasing human mineral intake to the daily requirements and the improvement of human health and the cost-effectiveness of the program is also important. In this paper, we review the available literature regarding the potential effectiveness and challenges of agronomic biofortification to improve crop micronutrient concentrations and reduce hidden hunger

Oat-field pea intercropping for sustainable oat production: effect on yield, nutritive value and environmental impact

The aim of the study is to evaluate the effect of Oat–field pea intercropping on the yield, nutritive value, and environmental impact of oat grown under a reduced level of nitrogen fertilisation. The trial was laid out in a randomized complete block design with the following treatments: oat-0 (oat (Avena sativa L., SRCP X 80 Ab 2291 variety) without N fertilization (urea)), oat-23 (oat fertilised with 23 kg N/ha), oat-46 (oat fertilised with 46 kg N/ha), O1P1 (oat intercropped with field pea (Pisum sativum L., local variety) a ratio of 1:1), O1P2 (oat intercropped with field pea a ratio of 1:2), and O2P1 (oat intercropped with field pea at a ratio of 2:1). All of the experimental plots received standard husbandry practices except for nitrogen fertilisation. Soil pH, organic matter, total nitrogen, available phosphorus, and organic carbon were determined before and after planting. The effect of nitrogen fertilization and intercropping of oat with field pea on carbon footprint, acidification footprint, eutrophication footprint, and human toxicity footprint was calculated for each plot. Oat-0 significantly reduced the total nitrogen content of the soil, while there was no significant effect of the other treatments. O2P1 significantly out-yielded all control groups; however, it was not significantly different from fertilisation treatments. Intercropping with field pea did not significantly increase the cost of production of dry matter, crude protein, or dry matter digestibility compared to control groups. Intercropping with field pea significantly reduced the carbon footprint, acidification, eutrophication, and human toxicity footprint compared to the control groups. Therefore, oat–field pea intercrops are recommended for the production of high-quality forage at low N input with reduced environmental impact