Health impacts of parental migration on left-behind children and adolescents: a systematic review and meta-analysis.

BACKGROUND: Globally, a growing number of children and adolescents are left behind when parents migrate. We investigated the effect of parental migration on the health of left behind-children and adolescents in low-income and middle-income countries (LMICs). METHODS: For this systematic review and meta-analysis we searched MEDLINE, Embase, CINAHL, the Cochrane Library, Web of Science, PsychINFO, Global Index Medicus, Scopus, and Popline from inception to April 27, 2017, without language restrictions, for observational studies investigating the effects of parental migration on nutrition, mental health, unintentional injuries, infectious disease, substance use, unprotected sex, early pregnancy, and abuse in left-behind children (aged 0-19 years) in LMICs. We excluded studies in which less than 50% of participants were aged 0-19 years, the mean or median age of participants was more than 19 years, fewer than 50% of parents had migrated for more than 6 months, or the mean or median duration of migration was less than 6 months. We screened studies using systematic review software and extracted summary estimates from published reports independently. The main outcomes were risk and prevalence of health outcomes, including nutrition (stunting, wasting, underweight, overweight and obesity, low birthweight, and anaemia), mental health (depressive disorder, anxiety disorder, conduct disorders, self-harm, and suicide), unintentional injuries, substance use, abuse, and infectious disease. We calculated pooled risk ratios (RRs) and standardised mean differences (SMDs) using random-effects models. This study is registered with PROSPERO, number CRD42017064871. FINDINGS: Our search identified 10 284 records, of which 111 studies were included for analysis, including a total of 264 967 children (n=106 167 left-behind children and adolescents; n=158 800 children and adolescents of non-migrant parents). 91 studies were done in China and focused on effects of internal labour migration. Compared with children of non-migrants, left-behind children had increased risk of depression and higher depression scores (RR 1·52 [95% CI 1·27-1·82]; SMD 0·16 [0·10-0·21]), anxiety (RR 1·85 [1·36-2·53]; SMD 0·18 [0·11-0·26]), suicidal ideation (RR 1·70 [1·28-2·26]), conduct disorder (SMD 0·16 [0·04-0·28]), substance use (RR 1·24 [1·00-1·52]), wasting (RR 1·13 [1·02-1·24]) and stunting (RR 1·12 [1·00-1·26]). No differences were identified between left-behind children and children of non-migrants for other nutrition outcomes, unintentional injury, abuse, or diarrhoea. No studies reported outcomes for other infectious diseases, self-harm, unprotected sex, or early pregnancy. Study quality varied across the included studies, with 43% of studies at high or unclear risk of bias across five or more domains. INTERPRETATION: Parental migration is detrimental to the health of left-behind children and adolescents, with no evidence of any benefit. Policy makers and health-care professionals need to take action to improve the health of these young people. FUNDING: Wellcome Trust

Infectious causes of microcephaly: epidemiology, pathogenesis, diagnosis, and management.

Microcephaly is an important sign of neurological malformation and a predictor of future disability. The 2015-16 outbreak of Zika virus and congenital Zika infection brought the world's attention to links between Zika infection and microcephaly. However, Zika virus is only one of the infectious causes of microcephaly and, although the contexts in which they occur vary greatly, all are of concern. In this Review, we summarise important aspects of major congenital infections that can cause microcephaly, and describe the epidemiology, transmission, clinical features, pathogenesis, management, and long-term consequences of these infections. We include infections that cause substantial impairment: cytomegalovirus, herpes simplex virus, rubella virus, Toxoplasma gondii, and Zika virus. We highlight potential issues with classification of microcephaly and show how some infants affected by congenital infection might be missed or incorrectly diagnosed. Although Zika virus has brought the attention of the world to the problem of microcephaly, prevention of all infectious causes of microcephaly and appropriately managing its consequences remain important global public health priorities

Purification of cross-linked RNA-protein complexes by phenol-toluol extraction

Recent methodological advances allowed the identification of an increasing number of RNA-binding proteins (RBPs) and their RNA-binding sites. Most of those methods rely, however, on capturing proteins associated to polyadenylated RNAs which neglects RBPs bound to non-adenylate RNA classes (tRNA, rRNA, pre-mRNA) as well as the vast majority of species that lack poly-A tails in their mRNAs (including all archea and bacteria). We have developed the Phenol Toluol extraction (PTex) protocol that does not rely on a specific RNA sequence or motif for isolation of cross-linked ribonucleoproteins (RNPs), but rather purifies them based entirely on their physicochemical properties. PTex captures RBPs that bind to RNA as short as 30 nt, RNPs directly from animal tissue and can be used to simplify complex workflows such as PAR-CLIP. Finally, we provide a global RNA-bound proteome of human HEK293 cells and the bacterium Salmonella Typhimurium

Temporal induction of Lhx8 by optogenetic control system for efficient bone regeneration

Abstract Background The spatiotemporal regulation of essential genes is crucial for controlling the growth and differentiation of cells in a precise manner during regeneration. Recently, optogenetics was considered as a potent technology for sophisticated regulation of target genes, which might be a promising tool for regenerative medicine. In this study, we used an optogenetic control system to precisely regulate the expression of Lhx8 to promote efficient bone regeneration. Methods Quantitative real-time PCR and western blotting were used to detect the expression of Lhx8 and osteogenic marker genes. Alkaline phosphatase staining and alizarin red staining were used to detect alkaline phosphatase activity and calcium nodules. A customized optogenetic expression system was constructed to regulate Lhx8, of which the expression was activated in blue light but not in dark. We also used a critical calvarial defect model for the analysis of bone regeneration in vivo. Moreover, micro-computed tomography (micro-CT), three-dimensional reconstruction, quantitative bone measurement, and histological and immunohistochemistry analysis were performed to investigate the formation of new bone in vivo. Results During the osteogenic differentiation of BMSCs, the expression levels of Lhx8 increased initially but then decreased thereafter. Lhx8 promoted the early proliferation of BMSCs but inhibited subsequent osteogenic differentiation. The optogenetic activation of Lhx8 in BMSCs in the early stages of differentiation by blue light stimulation led to a significant increase in cell proliferation, thus allowing a sufficient number of differentiating BMSCs to enter the later osteogenic differentiation stage. Analysis of the critical calvarial defect model revealed that the pulsed optogenetic activation of Lhx8 in transplanted BMSCs over a 5-day period led to a significant increase in the generation of bone in vivo. Conclusions Lhx8 plays a critical role in balancing proliferation and osteogenic differentiation in BMSCs. The optogenetic activation of Lhx8 expression at early stage of BMSCs differentiation led to better osteogenesis, which would be a promising strategy for precise bone regeneration

Sizing Optimization and Experimental Verification of a Hybrid Generation Water Pumping System in a Greenhouse

In remote agricultural areas, electrical energy is usually deficient for pumping water into greenhouses. Photovoltaic (PV) panels and wind generators are considered suitable options for power supply. The reliability of hybrid generation water pumping depends primarily on the number of system components, which should be adapted to the local climatic conditions and crop irrigation schedule. In this study, a universal size optimization model is established to optimize the configuration of a hybrid PV-wind-battery (PWB) generation system. The climatic conditions and crop irrigation schedule are parameterized in the model. Minimization of the annual cost of the hybrid PWB system is the objective function. The constraints include the battery state of charge (SOC) and the power supply reliability, which consists of the loss of power supply (δLPS) and the excess energy (δEX). The numbers of PV panels and batteries, as well as the rated power of the wind generator, are the decision variables. The optimization model of the PWB generation system is solved using a particle swarm optimization (PSO) algorithm based on penalty function. The model is then applied to determine the optimal configuration of a water pumping system for a greenhouse used to grow tomatoes. Measured climatic data are used in the optimization process, which is conducted in the month of maximum irrigation water requirement (August). The optimal results for this greenhouse are two PV panels and two batteries, and the rated power of the wind generator is 375 W. Furthermore, field experiments are performed to validate the optimization model. The field experiment results show that the total output power of the PV panels and wind generator during 15 d are 41.478 kW and 6.235 kW, respectively. The total load power of the pump is 36.965 kW. The field experiments demonstrate that the optimal results are able to meet the power requirements of the water pumping system and the sizing optimization model is appropriate

Experimental and Simulation Investigation on the Kinetic Energy Dissipation Rate of a Fixed Spray-Plate Sprinkler

Sprinkler irrigation is promoted due to its remarkable advantages in water conservation, but the high energy consumption limits its development in a situation of energy scarcity. In order to determine the energy consumption of a fixed spray-plate sprinkler (FSPS), its discharge and initial trajectory velocity were investigated using a particle image velocimetry (PIV) technique and computational fluid dynamics (CFD) analyses. A nozzle diameter of 4.76 mm was used under windless conditions. Overall, good agreement between simulation results and experimental values was obtained. On the premise that the simulation method produced high accuracy, a series of simulations was performed with different nozzle diameters. The water distribution pattern, stream trajectory velocity and kinetic energy dissipation were analyzed. The results show that the jet produced at the nozzle is split by grooves after it hits the plate, with separation occurring earlier with decreasing nozzle diameter. The area of the flow cross-section of the outlet is mainly influenced by nozzle diameter rather than working pressure. The initial trajectory velocity of the grooves increases logarithmically with increasing working pressure. A high working pressure may not cause large kinetic energy dissipation. The dissipation rate of the FSPS ranged from 28.01–50.97%, i.e., a large kinetic energy rate was observed. To reduce this energy dissipation and improve water use efficiency, the structure of the FSPS should be optimized in further research

Correction: Alectinib (CH5424802) antagonizes ABCB1- and ABCG2-mediated multidrug resistance in vitro, in vivo and ex vivo

An amendment to this paper has been published and can be accessed via a link at the top of the paper