Quantification of three macrolide antibiotics in pharmaceutical lots by HPLC: Development, validation and application to a simultaneous separation

A new validated high performance liquid chromatographic (HPLC) method with rapid analysis time and high efficiency, for the analysis of erythromycin, azithromycin and spiramycin, under isocratic conditions with ODB RP18 as a stationary phase is described. Using an eluent composed of acetonitrile –2-methyl-2-propanol –hydrogenphosphate buffer, pH 6.5, with 1.5% triethylamine (33:7: up to 100, v/v/v), delivered at a flow-rate of 1.0 mL min-1. Ultra Violet (UV) detection is performed at 210 nm. The selectivity is satisfactory enough and no problematic interfering peaks are observed. The procedure is quantitatively characterized and repeatability, linearity, detection and quantification limits are very satisfactory. The method is applied successfully for the assay of the studied drugs in pharmaceutical dosage forms as tablets and powder for oral suspension. Recovery experiments revealed recovery of 97.13–100.28%

Embryo transfer as a method to eliminate pathogenic agents in a rabbit colony

peer reviewedTo regain the SPF status of a contaminated but genetically valuable rabbit breeding unit, embryos from the contaminated does were transferred into SPF recipient females. Embryos were collected on day 3 of gestation by flushing uterine horns. All usable embryos were frozen, part of them were not transferred and kept in liquid nitrogen forming a stock of highly valuable genotypes. Thirty-two stimulated does produced 893 embryos, among which 821 (92%) had an intact zona pellucida and were cryopreserved. From this stock, 478 embryos were thawed, 466 were recovered (97.5%) and 417 were of good quality (87.2%). In 30 does, 10 to 18 embryos were surgically transferred per recipient doe and 24.9% (104/417) of them developed to term after transfer. This corresponds to an average number of 3.47 (104/30) live newborns per recipient. Health screenings performed on sanitized rabbits confirmed the disappearance of pathogenic agents

Differentiation of sympatric zebra and quagga mussels in ecotoxicological studies: A comparison of morphometric data, gene expression, and body metal concentrations

International audienc

Influence of metallic mixture exposure on subcellular metal distribution in Daphnia magna

International audienceAquatic ecosystems are constantly subjected to the introduction of anthropogenic pollutants which constitute a threat for living organisms. Metals are among the major contaminants, because of their persistence and may have short and long term deleterious effects. The whole body metal concentrations give useful information on the bioavailability of these compounds, but they are inadequate to predict their potential toxicity. As only a portion of incorporated metals is potentially toxic, the knowledge of the subcellular behaviour of metals in living organisms is fundamental. In several studies, authors show that effects were linked to the metal concentrations in the cytosolic fraction and particularly to the metal bound to low molecular weight compounds (Wang et al, 1999) or to high molecular weight compounds (Perceval et al, 2006). The aim of this project was to assess on one hand the metal distribution between cytosolic and insoluble fractions and on the other hand the cytosolic metal distribution according to three molecular mass class compounds including a fraction containing metallothionein proteins