18 research outputs found
Effects of elevated seawater pCO2 on gene expression patterns in the gills of the green crab, Carcinus maenas
Background: The green crab Carcinus maenas is known for its high acclimation potential to varying environmental
abiotic conditions. A high ability for ion and acid-base regulation is mainly based on an efficient regulation
apparatus located in gill epithelia. However, at present it is neither known which ion transport proteins play a key
role in the acid-base compensation response nor how gill epithelia respond to elevated seawater pCO2 as
predicted for the future. In order to promote our understanding of the responses of green crab acid-base
regulatory epithelia to high pCO2, Baltic Sea green crabs were exposed to a pCO2 of 400 Pa. Gills were screened
for differentially expressed gene transcripts using a 4,462-feature microarray and quantitative real-time PCR.
Results: Crabs responded mainly through fine scale adjustment of gene expression to elevated pCO2. However, 2%
of all investigated transcripts were significantly regulated 1.3 to 2.2-fold upon one-week exposure to CO2 stress.
Most of the genes known to code for proteins involved in osmo- and acid-base regulation, as well as cellular stress
response, were were not impacted by elevated pCO2. However, after one week of exposure, significant changes
were detected in a calcium-activated chloride channel, a hyperpolarization activated nucleotide-gated potassium
channel, a tetraspanin, and an integrin. Furthermore, a putative syntaxin-binding protein, a protein of the
transmembrane 9 superfamily, and a Cl-/HCO3
- exchanger of the SLC 4 family were differentially regulated. These
genes were also affected in a previously published hypoosmotic acclimation response study.
Conclusions: The moderate, but specific response of C. maenas gill gene expression indicates that (1) seawater
acidification does not act as a strong stressor on the cellular level in gill epithelia; (2) the response to hypercapnia
is to some degree comparable to a hypoosmotic acclimation response; (3) the specialization of each of the
posterior gill arches might go beyond what has been demonstrated up to date; and (4) a re-configuration of gill
epithelia might occur in response to hypercapnia
O trabalho como fonte de prazer: avaliação da equipe de um Centro de Atenção Psicossocial
O objetivo foi avaliar o prazer no trabalho de uma equipe de um Centro de Atenção Psicosocial. Trata-se de uma pesquisa qualitativa, tipo estudo de caso, utilizando Avaliação de Quarta Geração. Estudo realizado em Foz do Iguaçu, Paraná, em novembro e dezembro de 2006. Participaram 10 profissionais da equipe. Para a coleta de dados, foram utilizadas observação e entrevistas individuais. A análise teve início simultâneo à coleta de dados, e, para análise final, utilizaram-se os passos: ordenamento dos dados, classificação e análise final. Constituíram-se os seguintes temas de análise: características do trabalho no CAPS, sofrimento e enfrentamento do sofrimento no trabalho. No processo avaliativo, os trabalhadores demonstraram prazer e realização com seu trabalho manifestado no orgulho, realização e valorização daquilo que produzem. O prazer ocorre na construção da atenção psicossocial, pois no cotidiano há liberdade para rearranjar o seu modo operatório de trabalhar, possibilitando o desenvolvimento de atividades e atitudes capazes de lhe fornecer prazer