On ARS-interacting multifunctional protein p18

Nine aminoacyl-tRNA synthetases with three auxiliary components are forming a multisynthetase complex which is essential component of protein biosynthesis machinery. The smallest auxiliary component p18 takes part in biosynthesis channeling. It is also a regulator of p53 and has tumor suppressing function. Particular structure of this protein is still unknown. Here methods of structural bioinformatics were applied to generate a model of p18 spatial structure suitable for further research. The structure was created using homology modeling. DNA-binding residues of p18 were also derived both from raw sequence and from three-dimensional structure model

Modeling of ARS-interacting multifunctional proteins p18 and p38.

ARS-interacting multifunctional proteins (auxiliary components) – are nonezymatic proteins, associated with aminoacyl-tRNA synthetases – enzymes that catalyse the joining of amino acids with their corresponding tRNAs. All these proteins are complexed into large macromolecular multisynthetase complex in eukaryotes. It includes nine different ARSs and three auxiliary components. Their spatial structures precisely defined by X-ray analysis are still not known. Nevertheless, it very useful to have this structure while making biochemical studies. Bioinformatical tools were used to create structural models of two auxiliary components of multisynthetase complex – p18 and p38

Euclidean TSP with few inner points in linear space

Given a set of $n$ points in the Euclidean plane, such that just $k$ points are strictly inside the convex hull of the whole set, we want to find the shortest tour visiting every point. The fastest known algorithm for the version when $k$ is significantly smaller than $n$, i.e., when there are just few inner points, works in $O(k^{11\sqrt{k}} k^{1.5} n^{3})$ time [Knauer and Spillner, WG 2006], but also requires space of order $k^{c\sqrt{k}}n^{2}$. The best linear space algorithm takes $O(k! k n)$ time [Deineko, Hoffmann, Okamoto, Woeginer, Oper. Res. Lett. 34(1), 106-110]. We construct a linear space $O(nk^2+k^{O(\sqrt{k})})$ time algorithm. The new insight is extending the known divide-and-conquer method based on planar separators with a matching-based argument to shrink the instance in every recursive call. This argument also shows that the problem admits a quadratic bikernel.Comment: under submissio

OPTIMIZATION OF INTENSIVE THERAPY IN PATIENTS IN THE ACUTE PERIOD OF COMA

When prescribing intensive care to an unconscious patient, it is important to determine the etiological factor of the critical condition. The distribution of patients by neurotropic or vasotropic mechanism of disturbance of consciousness is extremely important for successful treatment and prevention of complications. Determination of autoantibodies to brain tissue is an important diagnostic criterion for the adequacy of intensive care. Administration of substances with succinic acid and D-fructose-1,6-diphosphate sodium salt hydrate is pathogenetically determined in this category of patients.When prescribing intensive care to an unconscious patient, it is important to determine the etiological factor of the critical condition. The distribution of patients by neurotropic or vasotropic mechanism of disturbance of consciousness is extremely important for successful treatment and prevention of complications. Determination of autoantibodies to brain tissue is an important diagnostic criterion for the adequacy of intensive care. Administration of substances with succinic acid and D-fructose-1,6-diphosphate sodium salt hydrate is pathogenetically determined in this category of patients

Suspension-cultured plant cells as a platform for obtaining recombinant proteins

Production of recombinant proteins in suspension cultures of genetically modified plant cells is a promising and rapidly developing area of plant biotechnology. In the present review article, advantages related to using plant systems for expression of recombinant proteins are considered. Here, the main focus is covering the literature on optimization of cultivation conditions of suspension-cultured plant cells to obtain a maximal yield of target proteins. In particular, certain examples of successful use of such cells to produce pharmaceuticals were described

Linearizable special cases of the QAP

We consider special cases of the quadratic assignment problem (QAP) that are linearizable in the sense of Bookhold. We provide combinatorial characterizations of the linearizable instances of the weighted feedback arc set QAP, and of the linearizable instances of the traveling salesman QAP. As a by-product, this yields a new well-solvable special case of the weighted feedback arc set problem