342 research outputs found
Circulating Tumor Cell Transcriptomics as Biopsy Surrogates in Metastatic Breast Cancer
BACKGROUND
Metastatic breast cancer (MBC) and the circulating tumor cells (CTCs) leading to macrometastases are inherently different than primary breast cancer. We evaluated whether whole transcriptome RNA-Seq of CTCs isolated via an epitope-independent approach may serve as a surrogate for biopsies of macrometastases.
METHODS
We performed RNA-Seq on fresh metastatic tumor biopsies, CTCs, and peripheral blood (PB) from 19 newly diagnosed MBC patients. CTCs were harvested using the ANGLE Parsortix microfluidics system to isolate cells based on size and deformability, independent of a priori knowledge of cell surface marker expression.
RESULTS
Gene expression separated CTCs, metastatic biopsies, and PB into distinct groups despite heterogeneity between patients and sample types. CTCs showed higher expression of immune oncology targets compared with corresponding metastases and PB. Predictive biomarker (n = 64) expression was highly concordant for CTCs and metastases. Repeat observation data post-treatment demonstrated changes in the activation of different biological pathways. Somatic single nucleotide variant analysis showed increasing mutational complexity over time.
CONCLUSION
We demonstrate that RNA-Seq of CTCs could serve as a surrogate biomarker for breast cancer macrometastasis and yield clinically relevant insights into disease biology and clinically actionable targets
Development of a PbWO4 Detector for Single-Shot Positron Annihilation Lifetime Spectroscopy at the GBAR Experiment
We have developed a PbWO4 (PWO) detector with a large dynamic range to measure the intensity of a positron beam and the absolute density of the ortho-positronium (o-Ps) cloud it creates. A simulation study shows that a setup based on such detectors may be used to determine the angular distribution of the emission and reflection of o-Ps to reduce part of the uncertainties of the measurement. These will allow to improve the precision in the measurement of the cross-section for the (anti)hydrogen formation by (anti)proton-positronium charge exchange and to optimize the yield of antihydrogen ion which is an essential parameter in the GBAR experiment
Observation of the rare decay K_S -> pi^0mu^+mu^-
A search for the decay K_S -> pi^0mu^+mu^- has been made by the NA48/1
Collaboration at the CERN SPS accelerator. The data were collected during 2002
with a high-intensity K_S beam. Six events were found with a background
expectation of 0.22^+0.18_-0.11 event. Using a vector matrix element and unit
form factor, the measured branching ratio is B(K_S ->
pi^0mu^+mu^-)=[2.9^+1.5_-1.2(stat)+/-0.2(syst)]x10^{-9}.Comment: 19 pages, 8 figures, 4 tables. To be published in Physics Letters
First observation of the KS->pi0 gamma gamma decay
Using the NA48 detector at the CERN SPS, 31 KS->pi0 gamma gamma candidates
with an estimated background of 13.7 +- 3.2 events have been observed. This
first observation leads to a branching ratio of BR(KS->pi0 gamma gamma) = (4.9
+- 1.6(stat) +- 0.9(syst)) x 10^-8 in agreement with Chiral Perturbation theory
predictions.Comment: 10 pages, 4 figures submitted to Phys. Lett.
Search for CP violation in K0 -> 3 pi0 decays
Using data taken during the year 2000 with the NA48 detector at the CERN SPS,
a search for the CP violating decay K_S -> 3 pi0 has been performed. From a fit
to the lifetime distribution of about 4.9 million reconstructed K0/K0bar -> 3
pi0 decays, the CP violating amplitude eta_000 = A(K_S -> 3 pi0)/A(K_L -> 3
pi0) has been found to be Re(eta_000) = -0.002 +- 0.011 +- 0.015 and
Im(eta_000) = -0.003 +- 0.013 +- 0.017. This corresponds to an upper limit on
the branching fraction of Br(K_S -> 3 pi0) < 7.4 x 10^-7 at 90% confidence
level. The result is used to improve knowledge of Re(epsilon) and the CPT
violating quantity Im(delta) via the Bell-Steinberger relation.Comment: 18 pages, 7 figures, submitted to Phys. Lett.
A precision measurement of direct CP violation in the decay of neutral kaons into two pions
The direct CP violation parameter Re(epsilon'/epsilon) has been measured from
the decay rates of neutral kaons into two pions using the NA48 detector at the
CERN SPS. The 2001 running period was devoted to collecting additional data
under varied conditions compared to earlier years (1997-99). The new data yield
the result: Re(epsilon'/epsilon) = (13.7 +/- 3.1) times 10^{-4}. Combining this
result with that published from the 1997, 98 and 99 data, an overall value of
Re(epsilon'/epsilon) = (14.7 +/- 2.2) times 10^{-4} is obtained from the NA48
experiment.Comment: 19 pages, 5 figures, to be published in Physics Letters
Measurement of the branching ratio of the decay
From the 2002 data taking with a neutral kaon beam extracted from the
CERN-SPS, the NA48/1 experiment observed 97 candidates with a background contamination of events.
From this sample, the BR() is measured to be
A new measurement of direct CP violation in two pion decays of the neutral kaon
The NA48 experiment at CERN has performed a new measurement of direct CP
violation, based on data taken in 1997 by simultaneously collecting K_L and K_S
decays into pi0pi0 and pi+pi-. The result for the CP violating parameter
Re(epsilon'/epsilon) is (18.5 +/- 4.5(stat)} +/- 5.8 (syst))x10^{-4}.Comment: 18 pages, 6 figure
First observation and branching fraction and decay parameter measurements of the weak radiative decay Xi0 --> Lambda e+e-
The weak radiative decay Xi0 --> Lambda e+e- has been detected for the first
time. We find 412 candidates in the signal region, with an estimated background
of 15 +/- 5 events. We determine the branching fraction B(Xi0 --> Lambda e+e-)
= [7.6 +/- 0.4(stat) +/- 0.4(syst) +/- 0.2(norm)] x 10^{-6}, consistent with an
internal bremsstrahlung process, and the decay asymmetry parameter
alpha_{XiLambdaee} = -0.8 +/- 0.2, consistent with that of Xi0 --> Lambda
gamma. The charge conjugate reaction Xi0_bar --> Lambda_bar e+e- has also been
observed.Comment: 20 pages, 5 figures, 4 tables; revised: 19 pages, 4 figures, 4
tables, after reviewers' comments: 1 figure removed, 1 figure corrected,
minor editorial changes; to be published in Phys. Lett.
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