34 research outputs found
Young adults in the workplace: A multisite initiative of substance use prevention programs
The Substance Abuse and Mental Health Services Administration funded the multisite Young Adults in the Workplace (YIW) initiative to study the effectiveness of diverse approaches to workplace-based prevention of substance abuse. Six teams adapted evidence-based programs to target young employees and then implemented the programs in retail, restaurant, health care, construction, skilled trade, and transportation industry workplaces. This book describes the programs, the adaptation and implementation processes, and the YIW cross-site evaluation
Does an Interactive Health Promotion Website Facilitate Workplace Peer-to-Peer Substance Abuse Prevention?
Operation RedBlock, a substance abuse prevention and early intervention program at Amtrak, implemented an interactive health promotion website (On The Right Track, OTRT) for its volunteers, other employees and their families. OTRT was customized from the Substance Abuse and Mental Health Services Administration (SAMHSA) -sponsored [email protected] website and implemented with the purpose of facilitating the peer-to-peer substance abuse prevention efforts of Operation RedBlock volunteers. SAMHSA’s GetFit website combines broad health and wellness messages with current substance abuse prevention resources. The OTRT customization adds Amtrak relevant and specific content concerning substance abuse prevention resources and treatment options, drug and alcohol use policies, health care coverage, support group meeting locations and dates, and local Operation RedBlock and Employee Assistance Program contact information. Using a retrospective pretest-posttest design this study evaluates whether and how OTRT facilitated peer-to-peer efforts in substance abuse prevention and early intervention. The evaluation revealed that the website significantly improved access to information and resources that helped Operation RedBlock volunteers’ in their peer-to-peer efforts. Volunteers valued the ready availability of reliable information, the confidential nature, and the ease of use of OTRT. Facilitated access to information and resources may help reduce substance abuse among employees at high risk
Recommended from our members
Organoid Sample Preparation and Extraction for LC-MS Peptidomics.
This protocol describes the peptidomic analysis of organoid lysates, FACS-purified cell populations, and 2D culture secretions by liquid chromatography mass spectrometry (LC-MS). Currently, most peptides are quantified by ELISA, limiting the peptides that can be studied. However, an LC-MS-based approach allows more peptides to be monitored. Our group has previously used LC-MS for tissue peptidomics and secretion of enteroendocrine peptides from primary culture. Now, we extend the use to organoid models. For complete details on the use and execution of this protocol, please refer to Goldspink et al. (2020)
Global Analysis of Protein N-Myristoylation and Exploration of N-Myristoyltransferase as a Drug Target in the Neglected Human Pathogen Leishmania donovani
N-Myristoyltransferase (NMT) modulates protein function through the attachment of the lipid myristate to the N terminus of target proteins, and is a promising drug target in eukaryotic parasites such as Leishmania donovani. Only a small number of NMT substrates have been characterized in Leishmania, and a global picture of N-myristoylation is lacking. Here, we use metabolic tagging with an alkyne-functionalized myristic acid mimetic in live parasites followed by downstream click chemistry and analysis to identify lipidated proteins in both the promastigote (extracellular) and amastigote (intracellular) life stages. Quantitative chemical proteomics is used to profile target engagement by NMT inhibitors, and to define the complement of N-myristoylated proteins. Our results provide new insight into the multiple pathways modulated by NMT and the pleiotropic effects of NMT inhibition. This work constitutes the first global experimental analysis of protein lipidation in Leishmania, and reveals the extent of NMT-related biology yet to be explored for this neglected human pathogen
Effect of cytomegalovirus infection on breastfeeding transmission of HIV and on the health of infants born to HIV-infected mothers
Cytomegalovirus (CMV) infection can be acquired in utero or postnatally through horizontal transmission and breastfeeding. The effect of postnatal CMV infection on postnatal HIV transmission is unknown
Plasma Micronutrient Concentrations Are Altered by Antiretroviral Therapy and Lipid-Based Nutrient Supplements in Lactating HIV-Infected Malawian Women
Background: Little is known about the influence of antiretroviral therapy with or without micronutrient supplementation on the micronutrient concentrations of HIV-infected lactating women in resource-constrained settings