10,522 research outputs found
Molecular Diagnostics in the Mycosphaerella Leaf Spot Disease Complex of Banana and for Radopholus similis
Mycosphaerella leaf spots and nematodes threaten banana cultivation worldwide. The Mycosphaerella disease complex involves three related ascomycetous fungi: Mycosphaerella fijiensis, M. musicola and M. eumusae. The exact distribution of these three species and their disease epidemiology remain unclear, since their symptoms and life cycles are rather similar. Diagnosing these diseases and the respective causal agents is based on the presence of host symptoms and fungal fruiting structures, but is time consuming and not conducive to preventive management. In the present study, we developed rapid and robust species-specific diagnostic tools to detect and quantify M. fijiensis, M. musicola and M. eumusae. Conventional species-specific PCR primers were developed based on the actin gene that detected as little as 100, 1 and 10 pg/µl DNA from, respectively, M. fijiensis, M. musicola and M. eumusae. Furthermore, TaqMan real-time quantitative PCR assays that were developed based on the ß-tubulin gene detected quantities as low as 1 pg/µl DNA of each species from pure cultures and 1.6 pg/µl DNA/mg of M. fijiensis from dry leaf tissue. The efficacy of the tests was validated using naturally infected banana leaves. Similar technology has been used to develop a quantitative PCR assay for the banana burrowing nematode, Radopholus similis, which is currently being validate
Nuclear medium effects in -nucleus deep inelastic scattering
We study the nuclear medium effects in the weak structure functions
and in the deep inelastic neutrino/antineutrino
reactions in nuclei.
We use a theoretical model for the nuclear spectral functions which
incorporates the conventional nuclear effects, such as Fermi motion, binding
and nucleon correlations.
We also consider the pion and rho meson cloud contributions calculated from a
microscopic model for meson-nucleus self-energies. The calculations have been
performed using relativistic nuclear spectral functions which include nucleon
correlations. Our results are compared with the experimental data of NuTeV and
CDHSW.Comment: 24 pages, 14 figure
Dosimetric benefits and preclinical performance of steerable needles in HDR prostate brachytherapy
Prostate cancer patients with an enlarged prostate and/or excessive pubic arch interference (PAI) are generally considered non-eligible for high-dose-rate (HDR) brachytherapy (BT). Steerable needles have been developed to make these patients eligible again. This study aims to validate the dosimetric impact and performance of steerable needles within the conventional clinical setting. HDR BT treatment plans were generated, needle implantations were performed in a prostate phantom, with prostate volume > 55 cm3 and excessive PAI of 10 mm, and pre- and post-implant dosimetry were compared considering the dosimetric constraints: prostate V100 > 95 % (13.50 Gy), urethra D0.1cm3 < 115 % (15.53 Gy) and rectum D1cm3 < 75 % (10.13 Gy). The inclusion of steerable needles resulted in a notable enhancement of the dose distribution and prostate V100 compared to treatment plans exclusively employing rigid needles to address PAI. Furthermore, the steerable needle plan demonstrated better agreement between pre- and post-implant dosimetry (prostate V100: 96.24 % vs. 93.74 %) compared to the rigid needle plans (79.13 % vs. 72.86 % and 87.70 % vs. 81.76 %), with no major changes in the clinical workflow and no changes in the clinical set-up. The steerable needle approach allows for more flexibility in needle positioning, ensuring a highly conformal dose distribution, and hence, HDR BT is a feasible treatment option again for prostate cancer patients with an enlarged prostate and/or excessive PAI.</p
Dosimetric benefits and preclinical performance of steerable needles in HDR prostate brachytherapy
Prostate cancer patients with an enlarged prostate and/or excessive pubic arch interference (PAI) are generally considered non-eligible for high-dose-rate (HDR) brachytherapy (BT). Steerable needles have been developed to make these patients eligible again. This study aims to validate the dosimetric impact and performance of steerable needles within the conventional clinical setting. HDR BT treatment plans were generated, needle implantations were performed in a prostate phantom, with prostate volume > 55 cm3 and excessive PAI of 10 mm, and pre- and post-implant dosimetry were compared considering the dosimetric constraints: prostate V100 > 95 % (13.50 Gy), urethra D0.1cm3 < 115 % (15.53 Gy) and rectum D1cm3 < 75 % (10.13 Gy). The inclusion of steerable needles resulted in a notable enhancement of the dose distribution and prostate V100 compared to treatment plans exclusively employing rigid needles to address PAI. Furthermore, the steerable needle plan demonstrated better agreement between pre- and post-implant dosimetry (prostate V100: 96.24 % vs. 93.74 %) compared to the rigid needle plans (79.13 % vs. 72.86 % and 87.70 % vs. 81.76 %), with no major changes in the clinical workflow and no changes in the clinical set-up. The steerable needle approach allows for more flexibility in needle positioning, ensuring a highly conformal dose distribution, and hence, HDR BT is a feasible treatment option again for prostate cancer patients with an enlarged prostate and/or excessive PAI.</p
Scaling of spontaneous rotation with temperature and plasma current in tokamaks
Using theoretical arguments, a simple scaling law for the size of the
intrinsic rotation observed in tokamaks in the absence of momentum injection is
found: the velocity generated in the core of a tokamak must be proportional to
the ion temperature difference in the core divided by the plasma current,
independent of the size of the device. The constant of proportionality is of
the order of . When the
intrinsic rotation profile is hollow, i.e. it is counter-current in the core of
the tokamak and co-current in the edge, the scaling law presented in this
Letter fits the data remarkably well for several tokamaks of vastly different
size and heated by different mechanisms.Comment: 5 pages, 3 figure
Long Term Variability of SDSS Quasars
We use a sample of 3791 quasars from the Sloan Digital Sky Survey (SDSS)
Early Data Release (EDR), and compare their photometry to historic plate
material for the same set of quasars in order to study their variability
properties. The time base-line we attain this way ranges from a few months to
up to 50 years. In contrast to monitoring programs, where relatively few
quasars are photometrically measured over shorter time periods, we utilize
existing databases to extend this base-line as much as possible, at the cost of
sampling per quasar. Our method, however, can easily be extended to much larger
samples. We construct variability Structure Functions and compare these to the
literature and model functions. From our modeling we conclude that 1) quasars
are more variable toward shorter wavelengths, 2) their variability is
consistent with an exponentially decaying light-curve with a typical time-scale
of ~2 years, 3) these outbursts occur on typical time-scales of ~200 years.
With the upcoming first data release of the SDSS, a much larger quasar sample
can be used to put these conclusions on a more secure footing.Comment: 16 pages, accepted for publication in AJ, Sept issu
Field-Dependent Tilt and Birefringence of Electroclinic Liquid Crystals: Theory and Experiment
An unresolved issue in the theory of liquid crystals is the molecular basis
of the electroclinic effect in the smectic-A phase. Recent x-ray scattering
experiments suggest that, in a class of siloxane-containing liquid crystals, an
electric field changes a state of disordered molecular tilt in random
directions into a state of ordered tilt in one direction. To investigate this
issue, we measure the optical tilt and birefringence of these liquid crystals
as functions of field and temperature, and we develop a theory for the
distribution of molecular orientations under a field. Comparison of theory and
experiment confirms that these materials have a disordered distribution of
molecular tilt directions that is aligned by an electric field, giving a large
electroclinic effect. It also shows that the net dipole moment of a correlated
volume of molecules, a key parameter in the theory, scales as a power law near
the smectic-A--smectic-C transition.Comment: 18 pages, including 9 postscript figures, uses REVTeX 3.0 and
epsf.st
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