Through-and-through femorofemoral wire retrograde technique for the E-nside precannulated inner branch thoracoabdominal stent graft

The E-nside multibranched stent graft (Jotec GmbH, Hechingen, Germany) is the first and only off-the-shelf thoracoabdominal prosthesis with precannulated inner branches. Usually, after the device deployment, target vessel stenting will be facilitated by antegrade inner branch cannulation to retrieve the precannulated guide. In the literature, the use of antegrade access has been associated with the potential risk of cerebral and systemic embolization. Therefore, other retrograde techniques have been described. We have reported a new retrograde approach using a precannulated through-and-through femorofemoral wire technique for target vessel catheterization

Annali storici di Principato Citra, A. 9, n. 1.1 (2011)

A.9, n.1.1(2011): G. Guardia, Editoriale, P. 3 ; A. Botti, D.L. Thurmond, F. La Greca, Un palmento ben conservato a Novi Velia ed altri palmenti nel territorio del Cilento. Osservazioni ed ipotesi, P. 5 ; G. Aromando, Una dipendenza cavense: Sant'Arsenio e la badia della SS. Trinità di Cava, P. 53 ; C. Schiavo, Avventure etimologiche in forma di prosa nelle terre del Cilento, P. 81 ; A. Capano, Caselle in Pittari: note storiche e il catasto murattiano del 1815, P. 104 ; A. Di Gennaro, Il porto romano di San Marco di Castellabate, P. 134 ; F. De Nigris, I comunisti in parrocchia: il Sessantotto cattolico in Italia, P. 147 ; S. De Divitiis, Il '68 dei cattolici: l'Azione cattolica a Salerno, P. 165 ; C. Schiavo, Santa Maria di Vito a Fogna, P. 176 ; P. Romanelli, La cappella dei SS. Pietro e Paolo nel palazzo vescovile di Novi Velia, P. 182 ; A. Tesauro, Vietri. Dalla guerra alla vigilia della Costituzione, P. 188

Pediatric Diabetic Nephropathy: Novel Insights from microRNAs

Diabetic nephropathy (DN) represents the most common microvascular complication in patients with diabetes. This progressive kidney disease has been recognized as the major cause of end-stage renal disease with higher morbidity and mortality. However, its tangled pathophysiology is still not fully known. Due to the serious health burden of DN, novel potential biomarkers have been proposed to improve early identification of the disease. In this complex landscape, several lines of evidence supported a critical role of microRNAs (miRNAs) in regulating posttranscriptional levels of protein-coding genes involved in DN pathophysiology. Indeed, intriguing data showed that deregulation of certain miRNAs (e.g., miRNAs 21, -25, -92, -210, -126, -216, and -377) were pathogenically linked to the onset and the progression of DN, suggesting not only a role as early biomarkers but also as potential therapeutic targets. To date, these regulatory biomolecules represent the most promising diagnostic and therapeutic options for DN in adult patients, while similar pediatric evidence is still limited. More, findings from these elegant studies, although promising, need to be deeper investigated in larger validation studies. In an attempt to provide a comprehensive pediatric overview in the field, we aimed to summarize the most recent evidence on the emerging role of miRNAs in pediatric DN pathophysiology

Diagnostic Clues in Pediatric Nutcracker Syndrome: From Two Clinical Cases to Current Literature Analysis

: Nutcracker syndrome (NCS) is a rare pediatric disease caused by left kidney vein compression. Besides the "Triade's symptoms", including hematuria, proteinuria, and flank pain, a wide spectrum of clinical manifestations has been reported. As the significant hemodynamic changes secondary to the dilatation of the left renal vein, serious consequences such as renal vein thrombosis and severe anemia might occur in these children. NCS diagnosis includes a variety of invasive and non-invasive imaging tools, but cutoff values need to be further validated. A conservative treatment represents the most common therapeutic approach for these patients, but operative options are available in selected cases. To complicate matters, a standard diagnostic and treatment algorithm is currently lacking and scientific pediatric evidence in this field is still poor and limited. In this perspective, early recognition of NCS is crucial but challenging for pediatricians. Therefore, a better knowledge of the disease is recommended. Starting from two different clinical presentations of NCS, we aimed to provide a comprehensive overview of the disease in children

Il Teatro dei Risvegli. Pratica creativa, cura e partecipazione sociale delle persone con esiti di coma

Il volume \ue8 dedicato agli ultimi tre anni di attivit\ue0 teatrale (2011-2013) della Compagnia Gli Amici di Luca e raccoglie scritti di Alessandro Bergonzoni, Nicola Bonazzi, Andrea Canevaro, Stefano Casi, Alessandra Cortesi, Fulvio De Nigris, Gerardo Guccini, Federica Perri, Maria Teresa Scassellati Sforzolini, Cristina Valenti, Antonio Vigan\uf2, Antonella Vigilante. Il Teatro dei Risvegli \ue8 un progetto sviluppato nell\u2019ambito della Casa dei Risvegli Luca De Nigris, struttura pubblica di riabilitazione e ricerca dell\u2019Azienda Usl di Bologna. La compagnia Gli amici di Luca, nata nel 2003, rappresenta l\u2019asse portante del progetto. Formata da persone che hanno vissuto l\u2019esperienza del coma, da operatori teatrali e da giovani attori, la compagnia \ue8 una realt\ue0 pressoch\ue9 unica in Italia e in Europa. Accanto all\u2019attivit\ue0 laboratoriale permanente, produce spettacoli avvalendosi del contributo di registi esterni, come Enzo Toma, Antonio Vigan\uf2, Enrico Castellani e Valeria Raimondi (Babilonia Teatri), Nicola Bonazzi (Teatro dell\u2019Argine), ed effettua tourn\ue9e in Italia e all\u2019estero. Negli anni Gli amici di Luca si sono progressivamente emancipati dalla connotazione di \u201cteatro sociale\u201d, per raggiungere esiti di indubbia professionalit\ue0 e inserirsi nella normale programmazione teatrale. A tutt\u2019oggi hanno realizzato otto spettacoli, ottenendo nel 2013 il premio nazionale della critica per "Pinocchio"

Lysosome purinergic receptor P2X4 regulates neoangiogenesis induced by microvesicles from sarcoma patients.

The tumor microenvironment modulates cancer growth. Extracellular vesicles (EVs) have been identified as key mediators of intercellular communication, but their role in tumor growth is largely unexplored. Here, we demonstrate that EVs from sarcoma patients promote neoangiogenesis via a purinergic X receptor 4 (P2XR4) -dependent mechanism in vitro and in vivo. Using a proteomic approach, we analyzed the protein content of plasma EVs and identified critical activated pathways in human umbilical vein endothelial cells (HUVECs) and human progenitor hematopoietic cells (CD34+). We then showed that vessel formation was due to rapid mitochondrial activation, intracellular Ca2+ mobilization, increased extracellular ATP, and trafficking of the lysosomal P2XR4 to the cell membrane, which is required for cell motility and formation of stable branching vascular networks. Cell membrane translocation of P2XR4 was induced by proteins and chemokines contained in EVs (e.g. Del-1 and SDF-1). Del-1 was found expressed in many EVs from sarcoma tumors and several tumor types. P2XR4 blockade reduced EVs-induced vessels in angioreactors, as well as intratumor vascularization in mouse xenografts. Together, these findings identify P2XR4 as a key mediator of EVs-induced tumor angiogenesis via a signaling mediated by mitochondria-lysosome-sensing response in endothelial cells, and indicate a novel target for therapeutic interventions

Lysosome purinergic receptor P2X4 regulates neoangiogenesis induced by microvesicles from sarcoma patients.

The tumor microenvironment modulates cancer growth. Extracellular vesicles (EVs) have been identified as key mediators of intercellular communication, but their role in tumor growth is largely unexplored. Here, we demonstrate that EVs from sarcoma patients promote neoangiogenesis via a purinergic X receptor 4 (P2XR4) -dependent mechanism in vitro and in vivo. Using a proteomic approach, we analyzed the protein content of plasma EVs and identified critical activated pathways in human umbilical vein endothelial cells (HUVECs) and human progenitor hematopoietic cells (CD34+). We then showed that vessel formation was due to rapid mitochondrial activation, intracellular Ca2+ mobilization, increased extracellular ATP, and trafficking of the lysosomal P2XR4 to the cell membrane, which is required for cell motility and formation of stable branching vascular networks. Cell membrane translocation of P2XR4 was induced by proteins and chemokines contained in EVs (e.g. Del-1 and SDF-1). Del-1 was found expressed in many EVs from sarcoma tumors and several tumor types. P2XR4 blockade reduced EVs-induced vessels in angioreactors, as well as intratumor vascularization in mouse xenografts. Together, these findings identify P2XR4 as a key mediator of EVs-induced tumor angiogenesis via a signaling mediated by mitochondria-lysosome-sensing response in endothelial cells, and indicate a novel target for therapeutic interventions