TEACHING AND PLAY PROPOSALS ON SOCIAL NETWORKS OF EARLY CHILHOOD EDUCATION, DURING THE COVID -19 PANDEMIC PERIOD.

Este estudo aborda as propostas de ensino que realizam as aulas da educação infantil durante o contexto de pandemia, especificamente abordando o jogo e o modo em que os docentes o apresentam nas redes sociais. A partir de uma metodologia qualitativa (STRAUSS; CORBIN, 2002), se analisaram intensivamente os posts realizados entre maio e outubro de 2021 em quatro escolas localizadas em diferentes bairros da área metropolitana de Buenos Aires (AMBA). Os resultados mostram que as escolas realizam postagens com diversos objetivos e conteúdo. A quantidade de posts de atividades lúdicas se diferencia segundo o “grau de formalização” das instituições (SIRVENT, 2008). Especificamente, os posts sobre jogo se caracterizam por apresentar diferentes tipos de atividades lúdicas e formas de ensino e orientação para as famílias por parte dos professores. Os dados fornecem evidências sobre os aspectos a ter em conta para dar passos na inclusão genuína do jogo (SARLÉ, 2017) e o lugar que tem a escola para enriquecer a experiência e potencializar a capacidade de jogar.This study addresses the teaching proposals built by early childhood education during the pandemic context, specifically taking into account play and the way in which teachers presented it, on social networks. Based on a qualitative methodology (STRAUSS; CORBIN, 2002), we analyze posts made between May and October 2021, in four schools located in different neighborhoods of the Buenos Aires metropolitan area (AMBA). The results show that schools make posts with various aims and contents. The amount of posts of playful activities differs according to the "degree of formalization" of the institutions (SIRVENT, 2008). Specifically, the posts about games were characterized by presenting different types of playful activities and ways of teaching as well as several guidelines for the families, all made by the teachers. The data offers evidence on the aspects to take into account to move towards the genuine inclusion of game (SARLÉ, 2017) and the place that assumes the pre-school as to enrich the experience and to enhance the possibility of playing.  Este estudio aborda las propuestas didácticas que las escuelas infantiles llevaron a cabo durante el contexto de la pandemia, tomando específicamente el juego y la forma en que los docentes los presentaban en las redes sociales. Con base en una metodología cualitativa (STRAUSS; CORBIN, 2002), se analizan intensamente las publicaciones realizadas entre mayo y octubre de 2021 en cuatro escuelas ubicadas en diferentes barrios del Área Metropolitana de Buenos Aires (AMBA). Los resultados muestran que las escuelas elaboran publicaciones con diferentes objetivos y contenidos. El número de puestos sobre actividades recreativas difiere según el “grado de formalización” de las instituciones (SIRVENT, 2008). Específicamente, las publicaciones sobre juegos, se caracterizan por presentar diferentes tipos de actividades lúdicas y formas de enseñanza y orientación a las familias por parte de los docentes. Los datos aportan evidencia sobre los aspectos a tener en cuenta para promover la inclusión genuina del juego (SARLÉ, 2017) y el lugar que ocupa la escuela para enriquecer la experiencia y potenciar la posibilidad de jugar

Aerobic biodegradation of butanol and diesel oil blends

This work aimed to evaluate the aerobic biodegradation of butanol/diesel oil blends (5, 10, 15, 20%, v/v) in comparison to the biodiesel/diesel oil blend (20%, v/v). Respirometric experiments simulating the contamination of natural environments (soil and water from a river) were carried out in biometer flasks (250 mL) used to measure microbial carbon dioxide (CO2) production. The automated turbidimeter Bioscreen C was used to follow the growth of Pseudomonas aeruginosa LBI on butanol/diesel oil blends. A redox indicator (2,6-dichlorophenol indophenol - DCPIP) test was used to evaluate the capability of four inocula to biodegrade the blends with 20% (v/v). The experiment which simulated the soil contamination demonstrated that butanol is less biodegradable than diesel oil, and for this reason the increase in the portion of butanol in the  butanol/diesel blend from 5 to 20% had negative effects on biodegradation. While in soil the biodiesel/diesel blend was more easily biodegraded than the butanol/diesel blend, in water this order was the inverse. The insoluble fuels (diesel and biodiesel) were poorly biodegraded in water and the biodegradation of the butanol/diesel blend was favored by the water solubilization of the butanol, which enhances the bioavailability of this compound. On the other hand, initial concentrations of butanol in the water higher than 10 mL L-1 inhibited the cell growth of the tested microorganisms. Thus, butanol toxicity presumably had a significant effect on the degree of biodegradation of the fuel blends.Key words: Butanol, biodiesel, diesel, biodegradation, blends, soil, water

Teaching and play proposals on social networks of early chilhood education, during the Covid -19 pandemic period

Este estudio aborda las propuestas de enseñanza que realizaron las escuelas infantiles durante el contexto de pandemia, específicamente tomando al juego y el modo en que los docentes lo presentaron en las redes sociales. A partir de una metodología cualitativa, se analizan intensivamente posts realizados entre mayo y octubre del 2021 en cuatro escuelas ubicadas en diferentes barrios del Área Metropolitana de Buenos Aires (AMBA). Los resultados muestran que las escuelas realizan posts con diversos objetivos y contenidos. La cantidad de posts de actividades lúdicas se diferencia según el “grado de formalización” de las instituciones. Específicamente, los posts sobre juego, se caracterizaron por presentar distintos tipos de actividades lúdicas y formas de enseñanza y orientación a las familias por parte de los docentes. Los datos ofrecen evidencia sobre los aspectos a tener en cuenta para promover la inclusión genuina del juego y el lugar que tiene la escuela al enriquecer la experiencia y potenciar la posibilidad de jugar.Este estudo aborda as propostas de ensino que realizam as aulas da educação infantil durante o contexto de pandemia, especificamente abordando a brincadeira e o modo em que os docentes o apresentam nas redes sociais. A partir de uma metodologia qualitativa, se analisaram intensivamente os posts realizados entre maio e outubro de 2021 em quatro escolas localizadas em diferentes bairros da área metropolitana de Buenos Aires (AMBA). Os resultados mostram que as escolas realizam postagens com diversos objetivos e conteúdo. A quantidade de posts de atividades lúdicas se diferencia segundo o “grau de formalização” das instituições. Especificamente, os posts sobre brincadeira se caracterizam por apresentar diferentes tipos de atividades lúdicas e formas de ensino e orientação para as famílias por parte dos professores. Os dados fornecem evidências sobre os aspectos a ter em conta para dar passos na inclusão genuína do jogo e o lugar que tem a escola para enriquecer a experiência e potencializar a capacidade de jogar.This study addresses the teaching proposals built by early childhood education during the pandemic context, specifically taking into account play and the way in which teachers presented it, on social networks. Based on a qualitative methodology, we analyze posts made between May and October 2021, in four schools located in different neighborhoods of the Buenos Aires metropolitan area (AMBA). The results show that schools make posts with various aims and contents. The amount of posts of playful activities differs according to the "degree of formalization" of the institutions. Specifically, the posts about play were characterized by presenting different types of playful activities and ways of teaching as well as several guidelines for the families, all made by the teachers. The data offers evidence on the aspects to take into account to move towards the genuine inclusion of game and the place that assumes the pre-school as to enrich the experience and to enhance the possibility of playing.Fil: Sarlé, Patricia Mónica. Universidad de Buenos Aires. Facultad de Filosofía y Letras. Instituto de Ciencias de la Educación; ArgentinaFil: Fernández, Andrea. Universidad de Buenos Aires. Facultad de Filosofía y Letras. Instituto de Ciencias de la Educación; ArgentinaFil: Ibañez, María Ileana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Saavedra 15. Centro Interdisciplinario de Investigaciones en Psicología Matemática y Experimental Dr. Horacio J. A. Rimoldi; ArgentinaFil: Monteverde, Ana Clara. Universidad de Buenos Aires. Facultad de Filosofía y Letras. Instituto de Ciencias de la Educación; ArgentinaFil: de Angelis, Susan Estrella. Universidad de Buenos Aires. Facultad de Filosofía y Letras. Instituto de Ciencias de la Educación; ArgentinaFil: Agullo, Marcela. Universidad de Buenos Aires. Facultad de Filosofía y Letras. Instituto de Ciencias de la Educación; Argentin

Characteristics of people living in Italy after a cancer diagnosis in 2010 and projections to 2020

BACKGROUND: Estimates of cancer prevalence are widely based on limited duration, often including patients living after a cancer diagnosis made in the previous 5 years and less frequently on complete prevalence (i.e., including all patients regardless of the time elapsed since diagnosis). This study aims to provide estimates of complete cancer prevalence in Italy by sex, age, and time since diagnosis for all cancers combined, and for selected cancer types. Projections were made up to 2020, overall and by time since diagnosis. METHODS: Data were from 27 Italian population-based cancer registries, covering 32% of the Italian population, able to provide at least 7 years of registration as of December 2009 and follow-up of vital status as of December 2013. The data were used to compute the limited-duration prevalence, in order to estimate the complete prevalence by means of the COMPREV software. RESULTS: In 2010, 2,637,975 persons were estimated to live in Italy after a cancer diagnosis, 1.2 million men and 1.4 million women, or 4.6% of the Italian population. A quarter of male prevalent cases had prostate cancer (n\u2009=\u2009305,044), while 42% of prevalent women had breast cancer (n\u2009=\u2009604,841). More than 1.5 million people (2.7% of Italians) were alive since 5 or more years after diagnosis and 20% since 6515 years. It is projected that, in 2020 in Italy, there will be 3.6 million prevalent cancer cases (+\u200937% vs 2010). The largest 10-year increases are foreseen for prostate (+\u200985%) and for thyroid cancers (+\u200979%), and for long-term survivors diagnosed since 20 or more years (+\u200945%). Among the population aged 6575 years, 22% will have had a previous cancer diagnosis. CONCLUSIONS: The number of persons living after a cancer diagnosis is estimated to rise of approximately 3% per year in Italy. The availability of detailed estimates and projections of the complete prevalence are intended to help the implementation of guidelines aimed to enhance the long-term follow-up of cancer survivors and to contribute their rehabilitation need

MET inhibition sensitizes rhabdomyosarcoma cells to NOTCH signaling suppression

Rhabdomyosarcoma (RMS) is a pediatric myogenic soft tissue sarcoma. The Fusion-Positive (FP) subtype expresses the chimeric protein PAX3-FOXO1 (P3F) while the Fusion-Negative (FN) is devoid of any gene translocation. FP-RMS and metastatic FN-RMS are often unresponsive to conventional therapy. Therefore, novel therapeutic approaches are needed to halt tumor progression. NOTCH signaling has oncogenic functions in RMS and its pharmacologic inhibition through gamma-secretase inhibitors blocks tumor growth in vitro and in vivo. Here, we show that NOTCH signaling blockade resulted in the up-regulation and phosphorylation of the MET oncogene in both RH30 (FP-RMS) and RD (FN-RMS) cell lines. Pharmacologic inhibition of either NOTCH or MET signaling slowed proliferation and restrained cell survival compared to control cells partly by increasing Annexin V and CASP3/7 activation. Co-treatment with NOTCH and MET inhibitors significantly amplified these effects and enhanced PARP1 cleavage in both cell lines. Moreover, it severely hampered cell migration, colony formation, and anchorage-independent growth compared to single-agent treatments in both cell lines and significantly prevented the growth of FN-RMS cells grown as spheroids. Collectively, our results unveil the overexpression of the MET oncogene by NOTCH signaling targeting in RMS cells and show that MET pathway blockade sensitizes them to NOTCH inhibition

Cure indicators and prevalence by stage at diagnosis for breast and colorectal cancer patients: A population‐based study in Italy

People alive many years after breast (BC) or colorectal cancer (CRC) diagnoses are increasing. This paper aimed to estimate the indicators of cancer cure and complete prevalence for Italian patients with BC and CRC by stage and age. A total of 31 Italian Cancer Registries (47% of the population) data until 2017 were included. Mixture cure models allowed estimation of net survival (NS); cure fraction (CF); time to cure (TTC, 5-year conditional NS >95%); cure prevalence (who will not die of cancer); and already cured (prevalent patients living longer than TTC). 2.6% of all Italian women (806,410) were alive in 2018 after BC and 88% will not die of BC. For those diagnosed in 2010, CF was 73%, 99% when diagnosed at stage I, 81% at stage II, and 36% at stages III-IV. For all stages combined, TTC was >10 years under 45 and over 65 years and for women with advanced stages, but <= 1 year for all BC patients at stage I. The proportion of already cured prevalent BC women was 75% (94% at stage I). Prevalent CRC cases were 422,407 (0.7% of the Italian population), 90% will not die of CRC. For CRC patients, CF was 56%, 92% at stage I, 71% at stage II, and 35% at stages III-IV. TTC was <= 10 years for all age groups and stages. Already cured were 59% of all prevalent CRC patients (93% at stage I). Cancer cure indicators by stage may contribute to appropriate follow-up in the years after diagnosis, thus avoiding patients' discrimination

Spermine oxidase induces DNA damage and sensitizes fusion negative rhabdomyosarcoma cells to irradiation

Rhabdomyosarcoma (RMS) is a pediatric myogenic soft tissue sarcoma that includes fusion-positive (FP) and fusion-negative (FN) molecular subtypes. FP-RMS expresses PAX3-FOXO1 fusion protein and often shows dismal prognosis. FN-RMS shows cytogenetic abnormalities and frequently harbors RAS pathway mutations. Despite the multimodal heavy chemo and radiation therapeutic regimens, high risk metastatic/recurrent FN-RMS shows a 5-year survival less than 30% due to poor sensitivity to chemo-radiotherapy. Therefore, the identification of novel targets is needed. Polyamines (PAs) such as putrescine (PUT), spermidine (SPD) and spermine (SPM) are low-molecular-mass highly charged molecules whose intracellular levels are strictly modulated by specific enzymes. Among the latter, spermine oxidase (SMOX) regulates polyamine catabolism oxidizing SPM to SPD, which impacts cellular processes such as apoptosis and DNA damage response. Here we report that low SMOX levels are associated with a worse outcome in FN-RMS, but not in FP-RMS, patients. Consistently, SMOX expression is downregulated in FN-RMS cell lines as compared to normal myoblasts. Moreover, SMOX transcript levels are reduced FN-RMS cells differentiation, being indirectly downregulated by the muscle transcription factor MYOD. Noteworthy, forced expression of SMOX in two cell lines derived from high-risk FN-RMS: 1) reduces SPM and upregulates SPD levels; 2) induces G0/G1 cell cycle arrest followed by apoptosis; 3) impairs anchorage-independent and tumor spheroids growth; 4) inhibits cell migration; 5) increases γH2AX levels and foci formation indicative of DNA damage. In addition, forced expression of SMOX and irradiation synergize at activating ATM and DNA-PKCs, and at inducing γH2AX expression and foci formation, which suggests an enhancement in DNA damage response. Irradiated SMOX-overexpressing FN-RMS cells also show significant decrease in both colony formation capacity and spheroids growth with respect to single approaches. Thus, our results unveil a role for SMOX as inhibitor of tumorigenicity of FN-RMS cells in vitro. In conclusion, our in vitro results suggest that SMOX induction could be a potential combinatorial approach to sensitize FN-RMS to ionizing radiation and deserve further in-depth studies

Invading Basement Membrane Matrix Is Sufficient for MDA-MB-231 Breast Cancer Cells to Develop a Stable In Vivo Metastatic Phenotype

1 - ArticleIntroduction: The poor efficacy of various anti-cancer treatments against metastatic cells has focused attention on the role of tumor microenvironment in cancer progression. To understand the contribution of the extracellular matrix (ECM) environment to this phenomenon, we isolated ECM surrogate invading cell populations from MDA-MB-231 breast cancer cells and studied their genotype and malignant phenotype. Methods: We isolated invasive subpopulations (INV) from non invasive populations (REF) using a 2D-Matrigel assay, a surrogate of basal membrane passage. INV and REF populations were investigated by microarray assay and for their capacities to adhere, invade and transmigrate in vitro, and to form metastases in nude mice. Results: REF and INV subpopulations were stable in culture and present different transcriptome profiles. INV cells were characterized by reduced expression of cell adhesion and cell-cell junction genes (44% of down regulated genes) and by a gain in expression of anti-apoptotic and pro-angiogenic gene sets. In line with this observation, in vitro INV cells showed reduced adhesion and increased motility through endothelial monolayers and fibronectin. When injected into the circulation, INV cells induced metastases formation, and reduced injected mice survival by up to 80% as compared to REF cells. In nude mice, INV xenografts grew rapidly inducing vessel formation and displaying resistance to apoptosis. Conclusion: Our findings reveal that the in vitro ECM microenvironment per se was sufficient to select for tumor cells with a stable metastatic phenotype in vivo characterized by loss of adhesion molecules expression and induction of proangiogenic and survival factors

AtlantECO Deliverable 2.1: AtlantECO-BASE1

This deliverable reports on Task 2.2 ‘Assembly of observations about microbiomes, plastics, the plastisphere and carbon fluxes’. It used protocols established in task 2.1 ‘Definition of common standards for the assembly of spatially explicit data’ to compile, quality-control and grid existing high-quality observations into a knowledge base of observations (D2.1). Data included into AtlantECO-BASE1 consisted of contributions from the five following data sources and tasks: Task 2.2.1 ‘Microbiome data from traditional microscopy (presence-absence, abundance and biomass)’, Task 2.2.2 ‘Microbiome data from state-of-the-art optical/imaging analysis’, Task 2.2.3 ‘Microbiome and plastisphere data from state-of-the-art genetic analyses’, Task 2.2.4 ‘Nano-, micro and macroplastics data from state-of-the-art sampling methods’, and Task 2.2.5 ‘Carbon flux data from estimated from high resolution bio-optical sensors’. Additional data contributions and mapping efforts from other partners and work packages (Task 2.3) are also included. A comprehensive list and description of all data sets collected can be found in the Appendix Tables to this document

Geographical heterogeneity of clinical and serological phenotypes of systemic sclerosis observed at tertiary referral centres. The experience of the Italian SIR-SPRING registry and review of the world literature

Introduction: Systemic sclerosis (SSc) is characterized by a complex etiopathogenesis encompassing both host genetic and environmental -infectious/toxic- factors responsible for altered fibrogenesis and diffuse microangiopathy. A wide spectrum of clinical phenotypes may be observed in patients' populations from different geographical areas. We investigated the prevalence of specific clinical and serological phenotypes in patients with definite SSc enrolled at tertiary referral centres in different Italian geographical macro-areas. The observed findings were compared with those reported in the world literature.Materials and methods: The clinical features of 1538 patients (161 M, 10.5%; mean age 59.8 +/- 26.9 yrs.; mean disease duration 8.9 +/- 7.7 yrs) with definite SSc recruited in 38 tertiary referral centres of the SPRING (Systemic sclerosis Progression INvestiGation Group) registry promoted by Italian Society of Rheumatology (SIR) were obtained and clustered according to Italian geographical macroareas.Results: Patients living in Southern Italy were characterized by more severe clinical and/or serological SSc phenotypes compared to those in Northern and Central Italy; namely, they show increased percentages of diffuse cutaneous SSc, digital ulcers, sicca syndrome, muscle involvement, arthritis, cardiopulmonary symptoms, interstitial lung involvement at HRCT, as well increased prevalence of serum anti-Scl70 autoantibodies. In the same SSc population immunusppressive drugs were frequently employed. The review of the literature underlined the geographical heterogeneity of SSc phenotypes, even if the observed findings are scarcely comparable due to the variability of methodological approaches.Conclusion: The phenotypical differences among SSc patients' subgroups from Italian macro-areas might be correlated to genetic/environmental co-factors, and possibly to a not equally distributed national network of information and healthcare facilities