On Restorative Validity: Reorienting Inquiry Toward Peace, Justice, and Healing

This work begins with a simple premise: (re)imagining a healing and restorative space for inquiry. Drawing on the work of John H. Stanfield II (2006), who first suggested the restorative functions of qualitative inquiry, this manuscript forms the basis for an axiologically-actuated conceptual model, restorative validity, which asks what it would take to (re)humanize researcher and researched alike. Beginning with the knowledge of co-researchers in our collective, the formulation of this framework was organized to understand the importance of orienting our research and ourselves toward relationships, justice, and liberation. After this review, I discuss a series of reflexive questions, rooted in the trans-disciplinarity of restorative justice, which researchers and practitioners can use to consider the potential and real harms in/from inquiry. By unsettling expertise and examining the implicit intersection of validity and ethics, I question: What would it take to be part of a research project that leaves those involved feeling greater than how we have all been defined? What happens when we do not question what our research does for/to us and our participants, especially when it spurs intellectual debate with little benefit in the way of peace, justice, or healing of past traumas and loss

Secretion-Positive LGI1 Mutations Linked to Lateral Temporal Epilepsy Impair Binding to ADAM22 and ADAM23 Receptors

Autosomal dominant lateral temporal epilepsy (ADTLE) is a focal epilepsy syndrome caused by mutations in the LGI1 gene, which encodes a secreted protein. Most ADLTE-causing mutations inhibit LGI1 protein secretion, and only a few secretion-positive missense mutations have been reported. Here we describe the effects of four disease-causing nonsynonymous LGI1 mutations, T380A, R407C, S473L, and R474Q, on protein secretion and extracellular interactions. Expression of LGI1 mutant proteins in cultured cells shows that these mutations do not inhibit protein secretion. This finding likely results from the lack of effects of these mutations on LGI1 protein folding, as suggested by 3D protein modelling. In addition, immunofluorescence and co-immunoprecipitation experiments reveal that all four mutations significantly impair interaction of LGI1 with the ADAM22 and ADAM23 receptors on the cell surface. These results support the existence of a second mechanism, alternative to inhibition of protein secretion, by which ADLTE-causing LGI1 mutations exert their loss-of-function effect extracellularly, and suggest that interactions of LGI1 with both ADAM22 and ADAM23 play an important role in the molecular mechanisms leading to ADLTE

Examining Researcher Identity Development within the Context of a Course on PAR: A Layered Narrative Approach

In this paper we explore the ways in which a group of doctoral students grapples with the epistemology of participatory action research (PAR) in relation to their own personal and professional identities and research agendas while taking a course on PAR. As a professor of research methodology and two doctoral students, we examine the entangled and often hidden processes of teaching and learning PAR in order to identify experiences or events that seem to prompt or deepen novice scholars’ understanding and foster confidence in their ability to enact the methodology themselves. Through analysis of participants’ course journals as a type of reflexive researcher identity development record, we draw on narrative inquiry and Carspecken’s concept of identity claims to systematically explore the participants’ experiences and trace their journeys as they encounter concerns about ethics, power dynamics, and the logistics of a “messy” methodology

Using a morpho-functional approach to assess phytoplankton dynamics in two adjacent high-mountain lakes: a 10-year survey

Colbricon Superiore and Inferiore are two small adjacent high-mountain lakes located in the Paneveggio Natural Park (Italy). The lakes differ by size and depth while sharing the same bedrock setting and catchment basin. Changes in the phytoplankton communities were studied over a 10-years period to individuate which environmental variables would determine the main differences in biotic assemblages across time and between the two lakes.The study was conducted with fortnightly samplings, assessing the density and biomass of algal taxa. Relationships of each of the biological variables with water temperature, pH, conductivity, transparency, water level, previous week rainfall, and relative water column stability were analyzed by correlation and regression analyses, cluster analysis, and by canonical correspondence analysis. The most significant variables resulted air temperature, hydrologic water level and pH. The smaller Colbricon Inferiore had about double the amount of phytoplankton density and biomass than did the larger Colbricon Superiore. The same lake had higher diversity and lower evenness in structure of the phytoplankton community. Notwithstanding their proximity each lake appears to follow independent species composition dynamics, however parallel patterns were interestingly revealed when data were analyzed by pooling taxa into morpho-functional groups. Morpho-functional groups (MFGs) 1b, 3a, 6b, 7a, 9b were differentially most abundant in warm periods, while 2c, 3b, 11c, 5e, 10a prevailed in cold years. MFGs 1b, 2d, 3a and 3b were more characteristic of Lake Colbricon Superiore, while Colbricon Inferiore preferentially featured MFGs 5a, 5e, 9a, 9b, 10a, 11a and 8a. The role of the meteo-climatic parameters was pointed out in driving the different patterns observed in the two lakes

Particle Analysis Pitfalls

This viewgraph presentation reviews the use of particle analysis to assist in preparing for the 4th Hubble Space Telescope (HST) Servicing mission. During this mission the Space Telescope Imaging Spectrograph (STIS) will be repaired. The particle analysis consisted of Finite element mesh creation, Black-body viewfactors generated using I-DEAS TMG Thermal Analysis, Grey-body viewfactors calculated using Markov method, Particle distribution modeled using an iterative Monte Carlo process, (time-consuming); in house software called MASTRAM, Differential analysis performed in Excel, and Visualization provided by Tecplot and I-DEAS. Several tests were performed and are reviewed: Conformal Coat Particle Study, Card Extraction Study, Cover Fastener Removal Particle Generation Study, and E-Graf Vibration Particulate Study. The lessons learned during this analysis are also reviewed

Semi-Automated Segmentation of Microbes in Color Images

ABSTRACT The goal of this work is to develop a system that can semi-automate the detection of multicolored foreground objects in digitized color images that contain complex and very noisy backgrounds. Although color image segmentation is considered a general problem, our application is microbiology where various colored stains are used to reveal information about the microbes without cultivation. Instead of providing a simple threshold, the proposed system offers an interactive environment whereby the user chooses multiple sample points to define the range of color pixels comprising the foreground microbes of interest. The system then uses the color and spatial distances of these target points to segment the microbes from the confusing background of pixels whose RGB values lie outside the newly defined range and finds the boundary of the foreground microbes using region-growing and mathematical morphology. Some other image processing methods are also applied to enhance the resultant image containing the colored microbes against a noise-free background. The prototype performs with 98% accuracy on a test set compared to manually edited ground truth data. The system described here will have many applications in image processing and analysis where one needs to segment typical pixel regions of similar but non-identical colors

Nodule Organogenesis and Symbiotic Mutants of the Model Legume \u3ci\u3eLotus japonicus\u3c/i\u3e

A detailed microscopical analysis of the morphological features that distinguish different developmental stages of nodule organogenesis in wild-type Lotus japonicus ecotype Gifu B-129-S9 plants was performed, to provide the necessary framework for the evaluation of altered phenotypes of L. japonicus symbiotic mutants. Subsequently, chemical ethyl methanesulfonate (EMS) mutagenesis of L. japonicus was carried out. The analysis of approximately 3,000 M1 plants and their progeny yielded 20 stable L. japonicus symbiotic variants, consisting of at least 14 different symbiosis- associated loci or complementation groups. Moreover, a mutation affecting L. japonicus root development was identified that also conferred a hypernodulation response when a line carrying the corresponding allele (LjEMS102) was inoculated with rhizobia. The phenotype of the LjEMS102 line was characterized by the presence of nodule structures covering almost the entire root length (Nod++), and by a concomitant inhibition of both root and stem growth. A mutation in a single nuclear gene was shown to be responsible for both root and symbiotic phenotypes observed in the L. japonicus LjEMS102 line, suggesting that (a) common mechanism(s) regulating root development and nodule formation exists in legumes

An Evaluation of Non-Uniform Grade Distribution with the Emergency Medicine Off-Service Standardized Letters of Evaluation

Introduction: Standardized Letters of Evaluation (SLOEs) are designed to objectively compare medical students to their peers for completed emergency medicine (EM) rotations to be used in the EM residency match. In an attempt to adapt quickly to the lack of availability of in-person EM rotations due to COVID restrictions, “off-service” SLOEs (OSLOEs) were allowed in place of traditional SLOEs. The purpose of this study was to assess the utility of OSLOEs for candidate selection during the 2020-21 application cycle at a single EM residency.Methods: A retrospective cohort review of all OSLOEs submitted during the 2020-21 academic year to an EM residency program was performed. A total of 270 OSLOES were eligible for review. Summary statistics were calculated for the study variables recorded, including global rank, grade, categorical details, and rank.Results: Of the 270 OSLOEs reviewed, 61.9% ranked candidates in the top 10% of their class, with 95% being ranked in the top two categories. Over 90% of students were graded as honors or high pass and over 75% of students were ranked in the top 1/3 for each specific OSLOE category.Conclusion: Our findings reveal questionable utility of the objective measures in the OSLOE as there are signs it may suffer from non-uniform grade distribution, leading to low utility for candidate selection. Our data shows marked over-ranking within the highest 2 categories. EM program directors and faculty should use caution as the OSLOE may not carry the same weight as a traditional SLOE when objectively evaluating prospectivestudents for a match into EM

CDX2 hox gene product in a rat model of esophageal cancer

<p>Abstract</p> <p>Background</p> <p>Barrett's mucosa is the precursor of esophageal adenocarcinoma. The molecular mechanisms behind Barrett's carcinogenesis are largely unknown. Experimental models of longstanding esophageal reflux of duodenal-gastric contents may provide important information on the biological sequence of the Barrett's oncogenesis.</p> <p>Methods</p> <p>The expression of <it>CDX2 </it>hox-gene product was assessed in a rat model of Barrett's carcinogenesis. Seventy-four rats underwent esophago-jejunostomy with gastric preservation. Excluding perisurgical deaths, the animals were sacrificed at various times after the surgical treatment (Group A: <10 weeks; Group B: 10–30 weeks; Group C: >30 weeks).</p> <p>Results</p> <p>No Cdx2 expression was detected in either squamous epithelia of the proximal esophagus or squamous cell carcinomas. <it>De novo </it>Cdx2 expression was consistently documented in the proliferative zone of the squamous epithelium close to reflux ulcers (Group A: 68%; Group B: 64%; Group C: 80%), multilayered epithelium and intestinal metaplasia (Group A: 9%; Group B: 41%; Group C: 60%), and esophageal adenocarcinomas (Group B: 36%; Group C: 35%). A trend for increasing overall Cdx2 expression was documented during the course of the experiment (<it>p </it>= 0.001).</p> <p>Conclusion</p> <p><it>De novo </it>expression of Cdx2 is an early event in the spectrum of the lesions induced by experimental gastro-esophageal reflux and should be considered as a key step in the morphogenesis of esophageal adenocarcinoma.</p