State of Harmonization of 24 Serum Albumin Measurement Procedures and Implications for Medical Decisions

BACKGROUND: Measurements of serum and plasma albumin are widely used in medicine, including as indicators of quality of patient care in renal dialysis centers. METHODS: Pools were prepared from residual patient serum (n = 50) and heparin plasma (n = 48) from patients without renal disease, and serum from patients with kidney failure before hemodialysis (n = 53). Albumin was measured in all samples and in ERM-DA470k/IFCC reference material (RM) by 3 immunochemical, 9 bromcresol green (BCG), and 12 bromcresol purple (BCP) methods. RESULTS: Two of 3 immunochemical procedures, 5 of 9 BCG, and 10 of 12 BCP methods recovered the RM value within its uncertainty. One immunochemical and 3 BCG methods were biased vs the RM value. Random error components were small for all measurement procedures. The Tina-quant immunochemical method was chosen as the reference measurement procedure based on recovery and results of error analyses. Mean biases for BCG vs Tina-quant were 1.5% to 13.9% and were larger at lower albumin concentrations. BCP methods\u27 mean biases were -5.4% to 1.2% irrespective of albumin concentration. Biases for plasma samples were generally higher than for serum samples for all method types. For most measurement procedures, biases were lower for serum from patients on hemodialysis vs patients without kidney disease. CONCLUSIONS: Significant differences among immunochemical, BCG, and BCP methods compromise interpretation of serum. albumin results. Guidelines and calculations for clinical management of kidney and other diseases must consider the method used for albumin measurement until harmonization can be achieved

On the Performance of Copying Large Files Across a Contention-Based Network

Analytical and simulation models of interconnected local area networks, because of the large scale involved, are often constrained to represent only the most ideal of conditions for tractability sake. Consequently, many of the important causes of network delay are not accounted for. In this study, experimental evidence is presented to show how delay time in local area networks is significantly affected by hardware limitations in the connected workstations, software overhead, and network contention. The mechanism is a controlled experiment with two Vax workstations over an Ethernet. We investigate the network delays for large file transfers, taking into account the Vax workstation disk transfer limitations; generalized file transfer software such as NFS, FTP, and rcp; and the effect of contention on this simple network by the introduction of substantial workload from competing workstations. A comparison is made between the experimental data and a network modeling tool, and the limitations of the tool are explained. Insights from these experiments have increased our understanding of how more complex networks are likely to perform under heavy workloads.http://deepblue.lib.umich.edu/bitstream/2027.42/107873/1/citi-tr-89-3.pd

Rapid magma generation or shared magmatic reservoir? Petrology and geochronology of the Rat Creek and Nelson Mountain Tuffs, CO, USA

This study was supported by the ETH Research Grant ETH-34 15-2 (JS).Large-volume silicic volcanism poses global hazards in the form of proximal pyroclastic density currents, distal ash fall and short-term climate perturbations, which altogether warrant the study of how silicic magma bodies evolve and assemble. The southern rocky mountain volcanic field (SRMVF) is home to some of the largest super-eruptions in the geological record, and has been studied to help address the debate over how quickly eruptible magma batches can be assembled–whether in decades to centuries, or more slowly over 100’s of kyr. The present study focuses on the San Luis caldera complex within the SRMVF, and discusses the paradigms of rapid magma generation vs. rapid magma assembly. The caldera complex consists of three overlapping calderas that overlie the sources of three large-volume mid-Cenozoic ignimbrites: first, the Rat Creek Tuff (RCT; zoned dacite-rhyolite, 150 km3), followed by the Cebolla Creek Tuff (mafic dacite, 250 km3) and finally, the Nelson Mountain Tuff (NMT; zoned dacite-rhyolite, 500 km3), which are all indistinguishable in age by 40Ar/39Ar dating. We argue for a shared magmatic history for the three units on the basis of (1) similar mineral trace element compositions in the first and last eruptions (plagioclase, sanidine, biotite, pyroxene, amphibole, titanite, and zircon), (2) overlapping zircon U-Pb ages in all three units, and (3) similar thermal rejuvenation signatures visible in biotite (low-Mn, high-Ba) and zircon (low-Hf, low-U) geochemistry within the RCT and NMT. It is postulated that the NMT was sourced from a pre-existing magma reservoir to the northeast, which is corroborated by the formation of the nearby Cochetopa Caldera during the eruption of the NMT. The inferred lateral magma transport has two important implications: (1) it demonstrates long-distance transport of highly viscosity magmas at volumes (100’s of km3) not previously recorded, and (2) the sourcing of magma from a nearby pre-existing magma reservoir greatly reduces the rate of magma generation necessary to explain the close coincidence of three overlapping, large-volume magma systems. Additionally, the concept of magmatic “flux” (km3 kyr−1) is discussed in this context, and it is argued that an area-normalized flux (km3 kyr–1 km−2) provides a more useful number for measuring magma production rates: it is expected that magmatic volumes will scale with footprint of the thermal anomaly, and not taking this into account may lead to errant volumetric flux (km3 kyr−1) estimates. Meanwhile, area-normalized flux estimates in a given area are similar between units, consistent with evolution in a relatively constant thermal regime. Such estimates also demonstrate similar fluxes for ∼cogenetic volcanic and plutonic units.Publisher PDFPeer reviewe

NetMod: A Design Tool for Large-Scale Heterogeneous Campus Networks

The Network Modeling Tool (NetMod) uses simple analytical models to provide the designers of large interconnected local area networks with an in-depth analysis of the potential performance of these systems. This tool can be used in either a university, industrial, or governmental campus networking environment consisting of thousands of computer sites. NetMod is implemented with a combination of the easy-to-use Macintosh software packages HyperCard and Excel. The objectives of NetMod, the analytical models, and the user interface are described in detail along with its application to an actual campus-wide network.http://deepblue.lib.umich.edu/bitstream/2027.42/107971/1/citi-tr-90-1.pd

User Profile and Workload Analysis for Local Area Networks

Performance analysis tools for computer networks need accurate and comprehensive estimates of user workload. An approach is presented that estimates network impact for a wide variety of end user types and applications that are typical on local area networks. Fourteen user types and nine generic application types are defined, and data is collected to determine the average network bandwidth needed to accommodate the output of individual and aggregate user/application combinations. Workload is estimated using a combination of data obtained from live test experiments, and data collected from the literature. Finally, the implementation of this data in a highly interactive network modeling tool (NetMod) is illustrated with screen images generated during tool execution.http://deepblue.lib.umich.edu/bitstream/2027.42/107870/1/citi-tr-90-3.pd

Scale-invariant magnetic anisotropy in RuCl3_3 at high magnetic fields

In RuCl$_3$, inelastic neutron scattering and Raman spectroscopy reveal a continuum of non-spin-wave excitations that persists to high temperature, suggesting the presence of a spin liquid state on a honeycomb lattice. In the context of the Kitaev model, magnetic fields introduce finite interactions between the elementary excitations, and thus the effects of high magnetic fields - comparable to the spin exchange energy scale - must be explored. Here we report measurements of the magnetotropic coefficient - the second derivative of the free energy with respect to magnetic field orientation - over a wide range of magnetic fields and temperatures. We find that magnetic field and temperature compete to determine the magnetic response in a way that is independent of the large intrinsic exchange interaction energy. This emergent scale-invariant magnetic anisotropy provides evidence for a high degree of exchange frustration that favors the formation of a spin liquid state in RuCl$_3$.Comment: arXiv admin note: substantial text overlap with arXiv:1901.09245. Nature Physic

Directional ballistic transport in the two-dimensional metal PdCoO2

In an idealized infinite crystal, the material properties are constrained by the symmetries of its unit cell. Naturally, the point-group symmetry is broken by the sample shape of any finite crystal, yet this is commonly unobservable in macroscopic metals. To sense the shape-induced symmetry lowering in such metals, long-lived bulk states originating from anisotropic Fermi surfaces are needed. Here we show how strongly facetted Fermi surfaces and long quasiparticle mean free paths present in microstructures of PdCoO2 yield an in-plane resistivity anisotropy that is forbidden by symmetry on an infinite hexagonal lattice. Bar shaped transport devices narrower than the mean free path are carved from single crystals using focused ion beam (FIB) milling, such that the ballistic charge carriers at low temperatures frequently collide with both sidewalls defining a channel. Two symmetry-forbidden transport signatures appear: the in-plane resistivity anisotropy exceeds a factor of 2, and transverse voltages appear in zero magnetic field. We robustly identify the channel direction as the source of symmetry breaking via ballistic Monte- Carlo simulations and numerical solution of the Boltzmann equation

Linking goniometer measurements to hyperspectral and multi-sensor imagery for retrieval of beach properties and coastal characterization

In June 2011, a multi-sensor airborne remote sensing campaign was flown at the Virginia Coast Reserve Long Term Ecological Research site with coordinated ground and water calibration and validation (cal/val) measurements. Remote sensing imagery acquired during the ten day exercise included hyperspectral imagery (CASI-1500), topographic LiDAR, and thermal infra-red imagery, all simultaneously from the same aircraft. Airborne synthetic aperture radar (SAR) data acquisition for a smaller subset of sites occurred in September 2011 (VCR\u2711). Focus areas for VCR\u2711 were properties of beaches and tidal flats and barrier island vegetation and, in the water column, shallow water bathymetry. On land, cal/val emphasized tidal flat and beach grain size distributions, density, moisture content, and other geotechnical properties such as shear and bearing strength (dynamic deflection modulus), which were related to hyperspectral BRDF measurements taken with the new NRL Goniometer for Outdoor Portable Hyperspectral Earth Reflectance (GOPHER). This builds on our earlier work at this site in 2007 related to beach properties and shallow water bathymetry. A priority for VCR\u2711 was to collect and model relationships between hyperspectral imagery, acquired from the aircraft at a variety of different phase angles, and geotechnical properties of beaches and tidal flats. One aspect of this effort was a demonstration that sand density differences are observable and consistent in reflectance spectra from GOPHER data, in CASI hyperspectral imagery, as well as in hyperspectral goniometer measurements conducted in our laboratory after VCR\u2711

Nucleic Acid Template and the Risk of a PCR-Induced HIV-1 Drug Resistance Mutation

The HIV-1 nucleoside RT inhibitor (NRTI)-resistance mutation, K65R confers intermediate to high-level resistance to the NRTIs abacavir, didanosine, emtricitabine, lamivudine, and tenofovir; and low-level resistance to stavudine. Several lines of evidence suggest that K65R is more common in HIV-1 subtype C than subtype B viruses.We performed ultra-deep pyrosequencing (UDPS) and clonal dideoxynucleotide sequencing of plasma virus samples to assess the prevalence of minority K65R variants in subtype B and C viruses from untreated individuals. Although UDPS of plasma samples from 18 subtype C and 27 subtype B viruses showed that a higher proportion of subtype C viruses contain K65R (1.04% vs. 0.25%; p<0.001), limiting dilution clonal sequencing failed to corroborate its presence in two of the samples in which K65R was present in >1.5% of UDPS reads. We therefore performed UDPS on clones and site-directed mutants containing subtype B- and C-specific patterns of silent mutations in the conserved KKK motif encompassing RT codons 64 to 66 and found that subtype-specific nucleotide differences were responsible for increased PCR-induced K65R mutation in subtype C viruses.This study shows that the RT KKK nucleotide template in subtype C viruses can lead to the spurious detection of K65R by highly sensitive PCR-dependent sequencing techniques. However, the study is also consistent with the subtype C nucleotide template being inherently responsible for increased polymerization-induced K65R mutations in vivo

Analysis of X.500 Distributed Directory Refresh Strategies

Distributed database directory refresh strategies, commonly recommended for the X.500 standard, are defined and analytically modeled for variations on push/pull and total/differential under idealistic asynchronous control conditions. The models are implemented in a HyperCard-based tool called DirMod (for "directory model"). Experimental test results show important elapsed time performance tradeoff among the different strategies, and live test data contribute to the verification of the models.http://deepblue.lib.umich.edu/bitstream/2027.42/107872/1/citi-tr-90-6.pd