State of Alaska Election Security Project Phase 2 Report

A laska’s election system is among the most secure in the country, and it has a number of safeguards other states are now adopting. But the technology Alaska uses to record and count votes could be improved— and the state’s huge size, limited road system, and scattered communities also create special challenges for insuring the integrity of the vote. In this second phase of an ongoing study of Alaska’s election security, we recommend ways of strengthening the system—not only the technology but also the election procedures. The lieutenant governor and the Division of Elections asked the University of Alaska Anchorage to do this evaluation, which began in September 2007.Lieutenant Governor Sean Parnell. State of Alaska Division of Elections.List of Appendices / Glossary / Study Team / Acknowledgments / Introduction / Summary of Recommendations / Part 1 Defense in Depth / Part 2 Fortification of Systems / Part 3 Confidence in Outcomes / Conclusions / Proposed Statement of Work for Phase 3: Implementation / Reference

The Realities of Foreign Humanitarianism and the U.S. Military: Nineteenth Century Roots

For nearly two centuries, United States (U.S.) political policy and non-government organizations (NGOs) had delivered foreign humanitarian assistance (FHA) when and where needed. The logistical capability of the professional U.S. military has allowed it to become an enduring delivery method for FHA. Since the 2000s, Congress has actively shaped the Department of Defense’s (DoD) role in humanitarian operations through a wide variety of authorities contained in the Armed Services (Title 10 U.S. Code) and Foreign Relations and Intercourse (Title 22 U.S. Code) statutes, and through annual legislation. Each branch of the military has also developed more defined doctrines in response to this increasing role in FHA. The U.S. army has developed the Army’s Technique Publication 3-57.20 in order to prepare for an increased humanitarian role. This thesis will challenge traditional notions of U.S. military FHA that places its origins either at the creation of the United Nations (UN) and the Truman Doctrine in the late 1940s, or at the Spanish-American War of 1898. That year, the American populace created a growing demand to right the wrongs committed by the seemingly inept and corrupt Spanish Empire. When action did finally come, the American people landed an army on the shores of a battered people. Incidentally, the United States did not land its troops on foreign soil in 1898 in the name of humanitarianism for the first time. No, decades of prior experience and custom fell alongside those troops. From aiding in the suppression of the Atlantic slave trade beginning in 1819 to providing order and medical attention to the people of Alexandria near the end of the century, the United States began a tradition of using its military for FHA that proves nearly as old as the nation itself

Collapse: The History of How We Fail, and What We Can Do To Save Ourselves

Using Diamond\u27s Five point framework for how societies have collapsed in the past allow us in the present to recognize these signs in modern societies. We have the gift of hindsight in which we now know that factors such as climate change, impact on the environment, decline on friendly contact, increase in hostile contact, and a conservative outlook have all contributed to past societies\u27 collapse. We now recognize these issues in modern societies such as China, Australia, Rwanda, and Haiti. This allows these societies to reevaluate their current environmental standards on whether they are compatible with their survival or collapse. These changes have been implemented in Australia, in which farmers are changing their irrigation systems to be compatible with the current nutrient levels of their soils and changing their grazing animal populations to be compatible with this soil degradation as well.https://scholarworks.moreheadstate.edu/celebration_posters_2023/1000/thumbnail.jp

Endogenous biosynthesis of n-3 long-chain PUFA in Atlantic salmon

A more efficient utilisation of marine derived sources of dietary omega-3 long-chain polyunsaturated fatty acids (n-3 LC PUFA) in cultured Atlantic salmon could, amongst other strategies, be facilitated by nutritional strategies that maximise endogenous n-3 LC PUFA synthesis. The objective of the current study was to quantify the extent of n-3 LC PUFA biosynthesis and the resultant effect on fillet nutritional quality in large, market size Atlantic salmon. Four diets were manufactured providing altered levels of dietary omega-3 substrate, namely 18:3n-3, and end-products, namely, 20:5n-3 and 22:6n-3. After 283 days of feeding, fish grew to in excess of 3000g and no differences in growth performance or biometrical parameters were recorded. An analysis of fatty acid composition and in vivo metabolism revealed that post-smolt Atlantic salmon have the potential to endogenously produce n-3 LC PUFA when provided with a substantial amount of dietary omega-3 substrate. Moreover, the extent of endogenous production resulted in fillet levels of n-3 LC PUFA comparable to fish fed a diet with added fish oil. Another major finding was that the presence of abundant dietary omega-3 substrate with the addition of dietary omega-3 end-product (i.e. fish oil) had a positive effect on final fillet levels of n-3 LC PUFA. This was likely the result of the preferential β-oxidation of dietary C18 n-3 PUFA resulting in an apparent conservation of n-3 LC PUFA from catabolism. Ultimately, this study highlights the potential for endogenous synthesis of n-3 LC PUFA to, at least partially, support a substantial reduction, in the amount of dietary fish oil in diets for market sized Atlantic salmon reared in seawater

The impact of dietary protein: lipid ratio on growth performance, fatty acid metabolism, product quality and waste output in Atlantic salmon (Salmo salar)

A common strategy for aquafeed manufacturers has been the utilisation of relatively large amounts of terrestrial, both animal and plant, oil sources to produce diets with a high energy content. The provision of high fat diets is aimed at promoting the utilisation of energy from lipid, thus increasing the amount of dietary protein used for tissue synthesis. However, in recent years the cost of marine sourced dietary lipids has risen, at the same time, farming operations are under increasing pressure to limit environmental degradation associated with nitrogenous waste effluent. Currently there is limited information available regarding the environmental and economic impacts of an altered dietary protein: lipid ratio in diets for large Atlantic salmon (Salmo salar) reared in seawater, presenting a potential impediment for nutritional based solutions. Accordingly the present study compared two isoenergetic diets with varied protein: lipid ratios via an assessment of growth, fatty acid utilisation, human nutritional quality, nitrogenous waste output and economic considerations. The trial diets were fed to the fish for the final 150 days of an on-farm grow-out period and resulted in minimal differences in fish growth, fatty acid utilisation and fillet quality. A decreased dietary protein: lipid ratio resulted in a more efficient protein utilisation both in terms of digestibility and assimilation into fish and, therefore, nitrogenous waste output was reduced. However, due to small differences in feed utilisation, the cost of fish production was numerically higher

Ocean acidification increases iodine accumulation in kelp-based coastal food webs

Kelp are main iodine accumulators in the ocean, and their growth and photosynthesis are likely to benefit from elevated seawater CO2 levels due to ocean acidification. However, there are currently no data on the effects of ocean acidification on iodine metabolism in kelp. As key primary producers in coastal ecosystems worldwide, any change in their iodine metabolism caused by climate change will potentially have important consequences for global geochemical cycles of iodine, including iodine levels of coastal food webs that underpin the nutrition of billions of humans around the world. Here, we found that elevated pCO2 enhanced growth and increased iodine accumulation not only in the model kelp Saccharina japonica using both short‐term laboratory experiment and long‐term in situ mesocosms, but also in several other edible and ecologically significant seaweeds using long‐term in situ mesocosms. Transcriptomic and proteomic analysis of S. japonica revealed that most vanadium‐dependent haloperoxidase genes involved in iodine efflux during oxidative stress are down‐regulated under increasing pCO2, suggesting that ocean acidification alleviates oxidative stress in kelp, which might contribute to their enhanced growth. When consumed by abalone (Haliotis discus), elevated iodine concentrations in S. japonica caused increased iodine accumulation in abalone, accompanied by reduced synthesis of thyroid hormones. Thus, our results suggest that kelp will benefit from ocean acidification by a reduction in environmental stress however, iodine levels in kelp‐based coastal food webs will increase, with potential impacts on biogeochemical cycles of iodine in coastal ecosystems

Altered levels of shorter vs long-chain omega-3 fatty acids in commercial diets for market-sized Atlantic salmon reared in seawater – Effects on fatty acid composition, metabolism and product quality

There is a growing trend of ‘replacing’ long-chain omega-3 polyunsaturated fatty acid (n-3 LC PUFA) rich oils with C18 shorter-chain omega-3 polyunsaturated fatty acid rich oils in Atlantic salmon aquafeed formulations. n-3 LC PUFA, including 20:5n-3 and 22:6n-3, play contrasting physiological roles and are metabolised differently in comparison to C18 PUFA. Accordingly, the present study recorded the effect of replacing n-3 LC PUFA rich dietary fish oil with C18 n-3 PUFA rich camelina oil at two inclusion levels in commercial-like diets fed to market-sized Atlantic salmon. This assessment was achieved by an analysis of industry relevant production parameters including growth performance, fatty acid composition and metabolism, nutrient digestibility and consumer acceptance (liking and attribute analysis of fillet). The trial was conducted over the final 150 days of an on-farm grow-out period in seawater. The dietary replacement of n-3 LC PUFA with C18 n-3 PUFA resulted in a significant decrease in fillet n-3 LC PUFA and a poorer growth performance. However, in the absence of fish oil, the inclusion of camelina oil at high levels (40%) contributed to an improved n-6/n-3 ratio and partially ameliorated low dietary n-3 LC PUFA by providing added substrate for endogenous n-3 LC PUFA synthesis in comparison to a 20% camelina oil inclusion. Furthermore, consumer acceptance of Atlantic salmon was unaffected by the dietary addition of camelina oil

Lithographic Patterning of Photoreactive Cell-Adhesive Proteins

We describe a novel, simple method for the photolithographic patterning of cell-adhesive proteins. Intrinsically photoreactive proteins are synthesized in Escherichia coli through incorporation of the non-canonical, photosensitive amino acid para-azidophenylalanine. Upon ultraviolet irradiation at 365 nm, proteins form cross-linked films with elastic moduli that can be tuned by varying the concentration of photoreactive amino acid in the expression medium. Films of these proteins can be directly patterned using standard photolithographic techniques. We demonstrate the utility of this method of protein patterning by creating stable arrays of fibroblast cells on an engineered protein “photoresist”