Evolutionary descent of prion genes from a ZIP metal ion transport ancestor

In the more than 20 years since its discovery, both the phylogenetic origin and cellular function of the prion protein (PrP) have remained enigmatic. Insights into the function of PrP may be obtained through a characterization of its molecular neighborhood. Quantitative interactome data revealed the spatial proximity of a subset of metal ion transporters of the ZIP family to mammalian prion proteins. A subsequent bioinformatic analysis revealed the presence of a prion-like protein sequence within the N-terminal, extracellular domain of a phylogenetic branch of ZIPs. Additional structural threading and ortholog sequence alignment analyses consolidated the conclusion that the prion protein gene family is phylogenetically derived from a ZIP-like ancestor molecule. Our data explain structural and functional features found within mammalian prion proteins as elements of an ancient involvement in the transmembrane transport of divalent cations. The connection to ZIP proteins is expected to open new avenues to elucidate the biology of the prion protein in health and disease

A double-diffusive interface tank for dynamic-response studies

Author Posting. © Sears Foundation for Marine Research, 2005. This article is posted here by permission of Sears Foundation for Marine Research for personal use, not for redistribution. The definitive version was published in Journal of Marine Research 63 (2005): 263-289, doi:10.1357/0022240053693842.A large tank capable of long-term maintenance of a sharp temperature-salinity interface has been developed and applied to measurements of the dynamical response of oceanographic sensors. A two-layer salt-stratified system is heated from below and cooled from above to provide two convectively mixed layers with a thin double-diffusive interface separating them. A temperature jump exceeding 10°C can be maintained over 1–2 cm (a vertical temperature gradient of order 103°C/m) for several weeks. A variable speed-lowering system allows testing of the dynamic response of conductivity and temperature sensors in full-size oceanographic instruments. An acoustic echo sounder and shadowgraph system provide nondisruptive monitoring of the interface and layer microstructure. Tests of several sensor systems show how data from the facility is used to determine sensor response times using several fitting techniques and the speed dependence of thermometer time constants is illustrated. The linearity of the conductivity–temperature relationship across the interface is proposed as a figure of merit for design of lag-correction filters to accurately match temperature and conductivity sensors for the computation of salinity. The effects of finite interface thickness, slow sensor sampling rates and the thermal mass of the conductivity cell are treated. Sensor response characterization is especially important for autonomous instruments where data processing and compression must be performed in-situ, but is also helpful in the development of new sensors and in assuring accurate salinity records from traditional wire-lowered and towed systems.This research was supported by the National Science Foundation, grants OCE-97-11869 and OCE-02-40956, NOAA CORC grant 154368 and a WHOI Mellon Technical Staff Award

Aspen biology, community classification, and management in the Blue Mountains

Quaking aspen (Populus tremuloides Michx.) is a valuable species that is declining in the Blue Mountains of northeastern Oregon. This publication is a compilation of over 20 years of aspen management experience by USDA Forest Service workers in the Blue Mountains. It includes a summary of aspen biology and occurrence in the Blue Mountains, and a discussion of aspen conservation and management techniques such as fencing, conifer removal, and artificial propagation. Local data on bird use of aspen stands, insects and diseases in aspen, and genetic studies of aspen are also included. An aspen community classification developed from over 200 sample plots is presented, with plant species composition and cover, environment and soils, and management considerations

Five Planets Transiting a Ninth Magnitude Star

The Kepler mission has revealed a great diversity of planetary systems and architectures, but most of the planets discovered by Kepler orbit faint stars. Using new data from the K2 mission, we present the discovery of a five planet system transiting a bright (V = 8.9, K = 7.7) star called HIP 41378. HIP 41378 is a slightly metal-poor late F-type star with moderate rotation (v sin(i) = 7 km/s) and lies at a distance of 116 +/- 18 from Earth. We find that HIP 41378 hosts two sub-Neptune sized planets orbiting 3.5% outside a 2:1 period commensurability in 15.6 and 31.7 day orbits. In addition, we detect three planets which each transit once during the 75 days spanned by K2 observations. One planet is Neptune sized in a likely ~160 day orbit, one is sub-Saturn sized likely in a ~130 day orbit, and one is a Jupiter sized planet in a likely ~1 year orbit. We show that these estimates for the orbital periods can be made more precise by taking into account dynamical stability considerations. We also calculate the distribution of stellar reflex velocities expected for this system, and show that it provides a good target for future radial velocity observations. If a precise orbital period can be determined for the outer Jovian planet through future observations, it will be an excellent candidate for follow-up transit observations to study its atmosphere and measure its oblateness.Comment: Accepted by ApJL. 12 pages, 6 figures, 2 table

Recombinant Human Bone Morphogenetic Protein-2 and Collagen for Bone Regeneration

The study reported describes a combination of recombinant human bone morphogenetic protein-2 (rhBMP-2) and collagen (C) to regenerate bone. Unilateral critical-sized defects (CSDs) were prepared in radii of 32 skeletally mature New Zealand white rabbits. Rabbits were divided evenly among four treatments: autograft, absorbable C (Helistatt), 35 mg of rhBMP-2 combined with absorbable C (rhBMP-2/C), and untreated CSDs. The two euthanasia periods were 4 and 8 weeks. Radiographs were taken the day of surgery, every 2 weeks, and at term and the percent of radiopacity was measured. Data analysis revealed a time-dependent increase in the percent radiopacity with rhBMP-2/C. Histological examination revealed the rhBMP-2/C treatment regenerated osseous contour by 8 weeks. According to quantitative histomorphometry, the CSD and C groups had significantly less new bone than either autograft or rhBMP-2/C (p ¡ 0.05). The results suggest that rhBMP-2/C could be an effective therapy to restore segmental bone defects

Near-infrared observations of water-ice in OH/IR stars

A search for the near-infrared water-ice absorption band was made in a number of very red OH/IR stars which are known to exhibit the 10um silicate absorption. As a by-product, accurate positions of these highly reddened objects are obtained. We derived a dust mass loss rate for each object by modelling the spectral energy distribution and the gas mass loss rate by solving the equation of motion for the dust drag wind. The derived mass loss rates show a strong correlation with the silicate optical depth as well as that of the water-ice. The stars have a high mass loss rate (> 1.0E-4 Msun/yr) with an average gas-to-dust mass ratio of 110. In objects which show the 3.1um water-ice absorption, the near-IR slope is much steeper than those with no water-ice. Comparison between our calculated mass loss rates and those derived from OH and CO observations indicates that these stars have recently increased their mass loss rates.Comment: 10 pages, 6 figures : accepted for publication in A&