72 research outputs found
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Mechanisms affecting the bioaccumulation of dietary quinoline by rainbow trout (Salmo gairdneri)
Factors that influenced the uptake, storage, and elimination of dietary quinoline by rainbow trout (Salmo gairdneri) were studied to obtain an understanding of the mechanisms affecting the bioaccumulation of dietary contaminants in teleosts. Rainbow trout readily absorbed ¹⁴C-quinoline from pelleted food (1% ration at 138 ug quinoline/g food) and most tissues reached apparent steady-state after 10 days feeding. Maximum whole-body concentrations of quinoline plus metabolites were only 30 ng/g after 7 days depuration. Uptake rate constants ranged from 0.00006/day for muscle to 0.1455/day for gallbladder plus bile. Mean elimination half-life for quinoline-derived radioactivity ranged from 0.4 days in gills to 8.7 days for muscle. Depending on tissue, 58-83% of the stored radioactivity was present as metabolites. About 14% of the radioactivity in the bile was present as glucuronide conjugates. Quinoline was absorbed from the stomach by rainbow trout and peak serum levels occurred 4-8 hr after a single feeding. Pharmacokinetics were described using a two-compartment body model with first-order absorption and disposition; estimated half-lives for the a and B phase were 4.1 and 54.1 hr, respectively. Depending on dose, 71 to 83% of the ingested radioactivity was excreted during the first 24 hr after feeding. Branchial excretion was the primary route of excretion, all other routes (fecal, biliary, urinary, dermal) contributing 99% of quinoline was available for absorption. About 60% of the residual body burden was stored in the gallbladder bile, but bile was retained only in starved fish. There was no evidence for enterohepatic circulation of quinoline or its metabolites following ejection of gallbladder bile. Increased feeding rates enhanced the movement of the food bolus and associated radioactivity through the intestine, but did not affect patterns of tissue disposition
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The Independent Technical Analysis Process
The Bonneville Power Administration (BPA) contracted with the Pacific Northwest National Laboratory (PNNL) to provide technical analytical support for system-wide fish passage information (BPA Project No. 2006-010-00). The goal of this project was to produce rigorous technical analysis products using independent analysts and anonymous peer reviewers. In the past, regional parties have interacted with a single entity, the Fish Passage Center to access the data, analyses, and coordination related to fish passage. This project provided an independent technical source for non-routine fish passage analyses while allowing routine support functions to be performed by other well-qualified entities
Ecological baseline study of the Yakima Firing Center proposed land acquisition: A Preliminary Report
A baseline census was conducted from October 1987 to Janurary 1988 on the property identified for possible expansion of the Yakima Firing Center. These studies provide general descriptions of the major plant communities presentand animal inhabitants during the late fall and winter study period. A vegetation map derived from a combination of onsite surveillance and remotely sensed imagery is also provided as part of this report. Through January 1988, 13 wildlife species of special interest to state and federal agencies, in addition to ducks and geese, were observed on the proposed expansion area. Then raptorial bird species were observed in the area, including bald eagles, golden eagles, and prairie falcons. Upland game bird species, such as sage grouse, California quail, chuckars, and gray (Hungarian) partridge were present. Loggerhead shrikes, a species of special interest, were also observed on the site. Estimates of waterfowl abundance are included for the Priest Rapids Pool of the Columbia River, which includes the proposed river crossing sites. The number of waterfowl on the proposed crossing areas were comparatively low during the winter of 1986 to 1987 and high in 1987 to 1988. Bald eagles ad common loons were observed on the crossing areas. Six small mammal species were captured during this study period;one, the sagebrush vole, is a species of special interest. Two large animal species, mule deer and elk, were noted on the site. Beaver were the only furbearig animals noted to date. Rainbow trout were the only fish species collected within the proposed northern expansion area. The distribution of fall chinook salmon spawning areas was documented within the proposed river crossing areas. 3 refs., 7 figs., 3 tabs
Ecological baseline study of the Yakima Firing Center proposed land acquisition: A status report
This report provides baseline environmental information for the property identified for possible expansion of the Yakima Firing Center. Results from this work provide general descriptions of the animals and major plant communities present. A vegetation map derived from a combination of on-site surveillance and remotely sensed imagery is provided as part of this report. Twenty-seven wildlife species of special interest (protected, sensitive, furbearer, game animal, etc.), and waterfowl, were observed on the proposed expansion area. Bird censuses revealed 13 raptorial species (including four of special interest: bald eagle, golden eagle, osprey, and prairie falcon); five upland game bird species (sage grouse, California quail, chukar, gray partridge, and ring-necked pheasant); common loons (a species proposed for state listing as threatened); and five other species of special interest (sage thrasher, loggerhead shrike, mourning dove, sage sparrow, and long-billed curlew). Estimates of waterfowl abundance are included for the Priest Rapids Pool of the Columbia River. Six small mammal species were captured during this study; one, the sagebrush vole, is a species of special interest. Two large animal species, mule deer and elk, were noted on the site. Five species of furbearing animals were observed (coyote, beaver, raccoon, mink, and striped skunk). Four species of reptiles and one amphibian were noted. Fisheries surveys were conducted to document the presence of gamefish, and sensitive-classified fish and aquatic invertebrates. Rainbow trout were the only fish collected within the boundaries of the proposed northern expansion area. 22 refs., 10 figs., 4 tabs
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Chromium Toxicity Test for Fall Chinook Salmon (Oncorhynchus tshawytscha) Using Hanford Site Groundwater: Onsite Early Life-Stage Toxicity Evaluation
The objective of this study was to evaluate site-specific effects for early life-stage (eyed eggs to free swimming juveniles) fall chinook salmon that might be exposed to hexavalent chromium from Hanford groundwater sources. Our exposure conditions included hexavalent chromium obtained from Hanford groundwater wells near the Columbia River, Columbia River water as the diluent, and locally adapted populations of fall chinook salmon. This report describes both a 96-hr pretest using rainbow trout eggs and an early life-stage test beginning with chinook salmon eggs
DOE Hydropower Program Biennial Report for FY 2005-2006
SUMMARY The U.S. Department of Energy (DOE) Hydropower Program is part of the Office of Wind and Hydropower Technologies, Office of Energy Efficiency and Renewable Energy. The Program's mission is to conduct research and development (R&D) that will increase the technical, societal, and environmental benefits of hydropower. The Department's Hydropower Program activities are conducted by its national laboratories: Idaho National Laboratory (INL) [formerly Idaho National Engineering and Environmental Laboratory], Oak Ridge National Laboratory (ORNL), Pacific Northwest National Laboratory (PNNL), and National Renewable Energy Laboratory (NREL), and by a number of industry, university, and federal research facilities. Programmatically, DOE Hydropower Program R&D activities are conducted in two areas: Technology Viability and Technology Application. The Technology Viability area has two components: (1) Advanced Hydropower Technology (Large Turbine Field Testing, Water Use Optimization, and Improved Mitigation Practices) and (2) Supporting Research and Testing (Environmental Performance Testing Methods, Computational and Physical Modeling, Instrumentation and Controls, and Environmental Analysis). The Technology Application area also has two components: (1) Systems Integration and Technology Acceptance (Hydro/Wind Integration, National Hydropower Collaborative, and Integration and Communications) and (2) Supporting Engineering and Analysis (Valuation Methods and Assessments and Characterization of Innovative Technology). This report describes the progress of the R&D conducted in FY 2005-2006 under all four program areas. Major accomplishments include the following: Conducted field testing of a Retrofit Aeration System to increase the dissolved oxygen content of water discharged from the turbines of the Osage Project in Missouri. Contributed to the installation and field testing of an advanced, minimum gap runner turbine at the Wanapum Dam project in Washington. Completed a state-of-the-science review of hydropower optimization methods and published reports on alternative operating strategies and opportunities for spill reduction. Carried out feasibility studies of new environmental performance measurements of the new MGR turbine at Wanapum Dam, including measurement of behavioral responses, biomarkers, bioindex testing, and the use of dyes to assess external injuries. Evaluated the benefits of mitigation measures for instream flow releases and the value of surface flow outlets for downstream fish passage. Refined turbulence flow measurement techniques, the computational modeling of unsteady flows, and models of blade strike of fish. Published numerous technical reports, proceedings papers, and peer-reviewed literature, most of which are available on the DOE Hydropower website. Further developed and tested the sensor fish measuring device at hydropower plants in the Columbia River. Data from the sensor fish are coupled with a computational model to yield a more detailed assessment of hydraulic environments in and around dams. Published reports related to the Virtual Hydropower Prospector and the assessment of water energy resources in the U.S. for low head/low power hydroelectric plants. Convened a workshop to consider the environmental and technical issues associated with new hydrokinetic and wave energy technologies. Laboratory and DOE staff participated in numerous workshops, conferences, coordination meetings, planning meetings, implementation meetings, and reviews to transfer the results of DOE-sponsored research to end-users
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Biological Assessment of the Advanced Turbine Design at Wanapum Dam, 2005
This report summarizes the results of studies sponsored by the U.S. Department of Energy and conducted by Pacific Northwest National Laboratory to evaluate the biological performance (likelihood of injury to fish) from an advanced design turbine installed at Unit 8 of Wanapum Dam on the Columbia River in Washington State in 2005. PNNL studies included a novel dye technique to measure injury to juvenile fish in the field, an evaluation of blade-strike using both deterministic and stochastic models, and extended analysis of the response of the Sensor Fish Device to strike, pressure, and turbulence within the turbine system. Fluorescein dye was used to evaluate injuries to live fish passed through the advanced turbine and an existing turbine at two spill discharges (15 and 17 kcfs). Under most treatments the results were not significantly different for the two turbines, however, eye injury occurred in nearly 30% of fish passing through Unit 9 but in less than 10% of those passing through Unit 8 at 15 kcfs. Both deterministic and stochastic blade-strike models were applied for the original and new AHTS turbines. The modeled probabilities were compared to the Sensor Fish results (Carlson et al. 2006) and the biological studies using juvenile fish (Normandeau et al. 2005) under the same operational parameters. The new AHTS turbine had slightly higher modeled injury rates than the original turbine, but no statistical evidence to suggest that there is significant difference in blade-strike injury probabilities between the two turbines, which is consistent with the experiment results using Sensor Fish and juvenile fish. PNNL also conducted Sensor Fish studies at Wanapum Dam in 2005 concurrent with live fish studies. The probablility of severe collision events was similar for both turbine. The advanced turbine had a slightly lower probability of severe shear events but a slightly higher probability of slight shear
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Laboratory Studies on the Effects of Shear on Fish
The overall objective of our studies was to specify an index describing the hydraulic force that fish experience when subjected to a shear environment. Fluid shear is a phenomenon that is important to fish. However, elevated levels of shear may result in strain rates that injure or kill fish. At hydroelectric generating facilities, concerns have been expressed that strain rates associated with passage through turbines, spillways, and fish bypass systems may adversely affect migrating fish. Development of fish friendly hydroelectric turbines requires knowledge of the physical forces (injury mechanisms) that impact entrained fish and the fish's tolerance to these forces. It requires up-front, pre-design specifications for the environmental conditions that occur within the turbine system, in other words, determining or assuming that those conditions known to injure fish will provide the descriptions of conditions that engineers must consider in the design of a turbine system. These biological specifications must be carefully and thoroughly documented throughout the design of a fish friendly turbine. To address the development of biological specifications, we designed and built a test facility where juvenile fish could be subjected to a range of shear environments and quantified their biological response
Coastal Upwelling Supplies Oxygen-Depleted Water to the Columbia River Estuary
Low dissolved oxygen (DO) is a common feature of many estuarine and shallow-water
environments, and is often attributed to anthropogenic nutrient enrichment from
terrestrial-fluvial pathways. However, recent events in the U.S. Pacific
Northwest have highlighted that wind-forced upwelling can cause naturally
occurring low DO water to move onto the continental shelf, leading to
mortalities of benthic fish and invertebrates. Coastal estuaries in the Pacific
Northwest are strongly linked to ocean forcings, and here we report observations
on the spatial and temporal patterns of oxygen concentration in the Columbia
River estuary. Hydrographic measurements were made from transect (spatial
survey) or anchor station (temporal survey) deployments over a variety of wind
stresses and tidal states during the upwelling seasons of 2006 through 2008.
During this period, biologically stressful levels of dissolved oxygen were
observed to enter the Columbia River estuary from oceanic sources, with minimum
values close to the hypoxic threshold of 2.0 mg L−1. Riverine
water was consistently normoxic. Upwelling wind stress controlled the timing and
magnitude of low DO events, while tidal-modulated estuarine circulation patterns
influenced the spatial extent and duration of exposure to low DO water. Strong
upwelling during neap tides produced the largest impact on the estuary. The
observed oxygen concentrations likely had deleterious behavioral and
physiological consequences for migrating juvenile salmon and benthic crabs.
Based on a wind-forced supply mechanism, low DO events are probably common to
the Columbia River and other regional estuaries and if conditions on the shelf
deteriorate further, as observations and models predict, Pacific Northwest
estuarine habitats could experience a decrease in environmental quality
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