Governing through partnership: Strategic migration partnerships and the politics of enrolment and ambivalence

This paper examines the role of partnerships in the governance of UK migration policy, considering how partnerships have been mobilised to navigate tensions between multiple state and non-state actors. The paper focuses on the work of regional strategic migration partnerships (SMPs) across the UK, bodies that bring together national and local government, statutory agencies, third sector organisations, and private contractors, all concerned with the management of migration and asylum. Considering SMPs as sites of collaboration and contention within local ‘battlegrounds’ of policy, the paper examines how enrolment and socialisation shape relations between actors, serving to sustain governmental authority but also offer openings for the formation of advocacy coalitions. Through examining how SMPs addressed the outsourcing of asylum support services, I argue that for many non-state actors enrolment into an SMP produces an ambivalent politics centred on forms of tactical closeness and critical discomfort. In concluding, I suggest the forms of intimacy created through enrolment serve to extend ambivalence as actors become torn between relationships and commitments, highlighting the fraught and often unstable nature of SMPs as bodies of policy coordination and advocacy potential

Cities of refuge Asylum and the politics of hospitality

This thesis draws upon ethnographic work in Sheffield, South Yorkshire, to interrogate asylum as a spatial experience. Arguing that the routine framing of asylum as either an issue of national securitisation or cosmopolitan and humanitarian ethics serves to overlook the visceral and prosaic practices of living asylum in contemporary Britain, this thesis develops an account of responding to asylum seekers through everyday life which is immersed in the tacit sociality, and spatiality, of the city. Through detailing the complex negotiations which emerge as asylum seekers encounter and create an array of spaces within the city, this thesis considers how accounts of sovereignty, welcome, charity and generosity are actively performed, worked upon and fractured within daily practices of hospitality. Here national accounts of 'domopolitics' and 'secure borders' intermingle and conflict with emergent modes of ethical sensibility, as individuals respond to asylum seekers through a series of shared spaces of encounter and accomplishment. Of central importance throughout these chapters is the need to take seriously both the unique and fragile experiences of space which form part of asylum as a lived experience, and the inherently negotiated, tentative and contextual nature of these spaces of asylum, riven as they are by differing visions of asylum, ethics and politics. Five chapters seek to document and approach these spaces of asylum as sites of affective belonging, and each draws together a range of accounts from social and political theory in order to engage with Sheffield's diverse politics of asylum. In doing so these chapters fuse a series of research encounters, engagements and events with an account of politics, ethics and social theory which is emergent from the contextual negotiations of the present. Achapter on Sheffield's past illustrates how national accounts of asylum as a begrudging act of welcome infuse the negotiations of the city with asylum seekers in the present. This is then counterposed in the following chapter by an account of Sheffield as a 'City of Sanctuary', built upon a micropolitics of cultural change and a recognition of the city's relational responsibilities. Three chapters then focus on specific spaces within the city. The first of these examines the spatial negotiations of two weekly drop-in centres for asylum seekers, suggesting that these may act as sites of ethical improvisation and tacit learning. The second extends these ethical developments into the public spaces of Sheffield, arguing that a minimal politics of access and 'small achievements' arises from the particular fusion of encounter, material and memory thrown up by being among others in the city. The third of these chapters considers the varied spaces of accommodation for asylum seekers in Sheffield, arguing that these act as key constraints on an affective connection to the city and to others. These chapters develop an account of asylum as a lived, practiced and felt experience not simply occurring in Sheffield, but occurring through Sheffield. Through these chapters I then develop a possible 'politics of becoming' for asylum seekers, grounded in the opportunity for mutual and generous encounters with others, a reassertion of sanctuary as a public good and a recognition of the relational responsibilities asylum as a spatial connection throws up

Draft Genome Sequence of Kocuria sp. Strain UCD-OTCP (Phylum Actinobacteria).

Here, we present the draft genome of Kocuria sp. strain UCD-OTCP, a member of the phylum Actinobacteria, isolated from a restaurant chair cushion. The assembly contains 3,791,485 bp (G+C content of 73%) and is contained in 68 scaffolds

Draft Genome Sequence of Curtobacterium flaccumfaciens Strain UCD-AKU (Phylum Actinobacteria).

Here we present the draft genome of an actinobacterium, Curtobacterium flaccumfaciens strain UCD-AKU, isolated from a residential carpet. The genome assembly contains 3,692,614 bp in 130 contigs. This is the first member of the Curtobacterium genus to be sequenced

Draft Genome Sequence of Dietzia sp. Strain UCD-THP (Phylum Actinobacteria).

Here, we present the draft genome sequence of an actinobacterium, Dietzia sp. strain UCD-THP, isolated from a residential toilet handle. The assembly contains 3,915,613 bp. The genome sequences of only two other Dietzia species have been published, those of Dietzia alimentaria and Dietzia cinnamea

Participation and wellbeing in urban greenspace: ‘curating sociability’ for refugees and asylum seekers

This paper examines how asylum seekers and refugees experience urban greenspaces. Whilst often overlooked in a focus on support services and integration, we argue that critically exploring the importance of urban greenspaces has wider implications for understanding how asylum seekers and refugees navigate experiences of displacement and resettlement. Drawing on empirical work foregrounding refugee experiences in Berlin, London, and Sheffield, we found that spending time outdoors in local recreational spaces such as parks, can have positive outcomes for wellbeing and inclusion, with the potential to support respite and the beginnings of belonging. However, though there were multiple positive accounts, especially of busier parks and of appreciating nature, many participants were uncertain or anxious about using parks. The interviews highlight the multiple barriers faced by asylum seekers and refugees, regarding information, legibility and in gaining the cultural capital and confidence needed venture out. The varied experiences reflect the diversity of greenspace typologies in Northern European cities, and also how individuals weight up public perceptions and, for some, the insecurity of their legal status. In unpacking the interaction between these barriers, we define and propose ‘curated sociability’ approaches as possible frameworks for supporting egalitarian participation and offering pathways to greater engagement. We conclude by highlighting a range of interventions that offer situated opportunities for asylum seekers and refugees to engage with urban greenspaces, and which provide insights into how the expectations and rules of urban greenspace are actively negotiated and may be rewritten

Mauve Assembly Metrics

Summary: High-throughput DNA sequencing technologies have spurred the development of numerous novel methods for genome assembly. With few exceptions, these algorithms are heuristic and require one or more parameters to be manually set by the user. One approach to parameter tuning involves assembling data from an organism with an available high-quality reference genome, and measuring assembly accuracy using some metrics

Metagenomic Sequencing of an In Vitro-Simulated Microbial Community

Background: Microbial life dominates the earth, but many species are difficult or even impossible to study under laboratory conditions. Sequencing DNA directly from the environment, a technique commonly referred to as metagenomics, is an important tool for cataloging microbial life. This culture-independent approach involves collecting samples that include microbes in them, extracting DNA from the samples, and sequencing the DNA. A sample may contain many different microorganisms, macroorganisms, and even free-floating environmental DNA. A fundamental challenge in metagenomics has been estimating the abundance of organisms in a sample based on the frequency with which the organism's DNA was observed in reads generated via DNA sequencing. Methodology/Principal Findings: We created mixtures of ten microbial species for which genome sequences are known. Each mixture contained an equal number of cells of each species. We then extracted DNA from the mixtures, sequenced the DNA, and measured the frequency with which genomic regions from each organism was observed in the sequenced DNA. We found that the observed frequency of reads mapping to each organism did not reflect the equal numbers of cells that were known to be included in each mixture. The relative organism abundances varied significantly depending on the DNA extraction and sequencing protocol utilized. Conclusions/Significance: We describe a new data resource for measuring the accuracy of metagenomic binning methods, created by in vitro-simulation of a metagenomic community. Our in vitro simulation can be used to complement previous in silico benchmark studies. In constructing a synthetic community and sequencing its metagenome, we encountered several sources of observation bias that likely affect most metagenomic experiments to date and present challenges for comparative metagenomic studies. DNA preparation methods have a particularly profound effect in our study, implying that samples prepared with different protocols are not suitable for comparative metagenomics