LEGALIZACIÓN DEL USO DE ARMAS DE FUEGO PARA EL PERSONAL DE SERENAZGO DE LA MUNICIPALIDAD PROVINCIAL DE CHICLAYO

La presente investigación ha sido titulada “Legalización del Uso de Armas de Fuego para el Personal de Serenazgo de la Municipalidad Provincial de Chiclayo”, teniendo como variables del problema empirismos normativos y discrepancias teóricas, además tenemos como variables de la realidad a los operadores del derecho, la gerencia de seguridad ciudadana y personal de Serenazgo y como variables del marco referencial tenemos a los conceptos básicos, normas y la legislación comparada, que nos ha permitido poder formular la propuesta como solución del problema, es decir un proyecto de ley, como hipótesis se ha podido formular, la Legalización del uso de armas de fuego para el personal de serenazgo de la municipalidad provincial de Chiclayo se ha visto afectada por empirismos normativos y discrepancias teóricas, debido a que no se tuvo en cuenta a las variables antes mencionadas, como objetivo principal nos hemos planteado, analizar la legalización de armas de fuego para el personal de Serenazgo de la Municipalidad provincial de Chiclayo, exclusivamente para aquellos que prestan el servicio en las calles, más no para lo que realizan una labor administrativa.Tesi

LEGALIZACIÓN DEL USO DE ARMAS DE FUEGO PARA EL PERSONAL DE SERENAZGO DE LA MUNICIPALIDAD PROVINCIAL DE CHICLAYO

TesisLa presente investigación ha sido titulada “Legalización del Uso de Armas de Fuego para el Personal de Serenazgo de la Municipalidad Provincial de Chiclayo”, teniendo como variables del problema empirismos normativos y discrepancias teóricas, además tenemos como variables de la realidad a los operadores del derecho, la gerencia de seguridad ciudadana y personal de Serenazgo y como variables del marco referencial tenemos a los conceptos básicos, normas y la legislación comparada, que nos ha permitido poder formular la propuesta como solución del problema, es decir un proyecto de ley, como hipótesis se ha podido formular, la Legalización del uso de armas de fuego para el personal de serenazgo de la municipalidad provincial de Chiclayo se ha visto afectada por empirismos normativos y discrepancias teóricas, debido a que no se tuvo en cuenta a las variables antes mencionadas, como objetivo principal nos hemos planteado, analizar la legalización de armas de fuego para el personal de Serenazgo de la Municipalidad provincial de Chiclayo, exclusivamente para aquellos que prestan el servicio en las calles, más no para lo que realizan una labor administrativa

Isolation and Characterization of Cat Olfactory Ecto-Mesenchymal Stem Cells

The olfactory mucosa contains olfactory ecto-mesenchymal stem cells (OE-MSCs) which show stemness features, multipotency capabilities, and have a therapeutic potential. The OE-MSCs have already been collected and isolated from various mammals. The aim of this study was to evaluate the feasibility of collecting, purifying and amplifying OE-MSCs from the cat nasal cavity. Four cats were included in the study. Biopsies of olfactory mucosa were performed on anesthetized animals. Then, the olfactory OE-MSCs were isolated, and their stemness features as well as their mesodermal differentiation capabilities were characterized. Olfactory mucosa biopsies were successfully performed in all subjects. From these biopsies, cellular populations were rapidly generated, presenting various stemness features, such as a fibroblast-like morphology, nestin and MAP2 expression, and sphere and colony formation. These cells could differentiate into neural and mesodermal lineages. This report shows for the first time that the isolation of OE-MSCs from cat olfactory mucosa is possible. These cells showed stemness features and multilineage differentiation capabilities, indicating they may be a promising tool for autologous grafts and feline regenerative medicine

Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera

International audienceBackground: Stem cell-based therapies are an attractive option to promote regeneration and repair defective tissues and organs. Thanks to their multipotency, high proliferation rate and the lack of major ethical limitations, "olfactory ecto-mesenchymal stem cells" (OE-MSCs) have been described as a promising candidate to treat a variety of damaged tissues. Easily accessible in the nasal cavity of most mammals, these cells are highly suitable for autologous cell-based therapies and do not face issues associated with other stem cells. However, their clinical use in humans and animals is limited due to a lack of preclinical studies on autologous transplantation and because no well-established methods currently exist to cultivate these cells. Here we evaluated the feasibility of collecting, purifying and amplifying OE-MSCs from different mammalian genera with the goal of promoting their interest in veterinary regenerative medicine. Biopsies of olfactory mucosa from eight mammalian genera (mouse, rat, rabbit, sheep, dog, horse, gray mouse lemur and macaque) were collected, using techniques derived from those previously used in humans and rats. The possibility of amplifying these cells and their stemness features and differentiation capability were then evaluated. Results: Biopsies were successfully performed on olfactory mucosa without requiring the sacrifice of the donor animal, except mice. Cell populations were rapidly generated from olfactory mucosa explants. These cells displayed similar key features of their human counterparts: a fibroblastic morphology, a robust expression of nestin, an ability to form spheres and similar expression of surface markers (CD44, CD73). Moreover, most of them also exhibited high proliferation rates and clonogenicity with genus-specific properties. Finally, OE-MSCs also showed the ability to differentiate into mesodermal lineages. Conclusions: This article describes for the first time how millions of OE-MSCs can be quickly and easily obtained from different mammalian genera through protocols that are well-suited for autologous transplantations. Moreover, their multipotency makes them relevant to evaluate therapeutic application in a wide variety of tissue injury models. This study paves the way for the development of new fundamental and clinical studies based on OE-MSCs transplantation and suggests their interest in veterinary medicine

Prognostic value of high-sensitivity measurable residual disease assessment after front-line chemoimmunotherapy in chronic lymphocytic leukemia

International audienceMeasurable residual disease (MRD) status is widely adopted in clinical trials in patients with chronic lymphocytic leukemia (CLL). Findings from FILO group trials (CLL2007FMP, CLL2007SA, CLL2010FMP) enabled investigation of the prognostic value of high-sensitivity (0.7 × 10-5) MRD assessment using flow cytometry, in blood (N = 401) and bone marrow (N = 339), after fludarabine, cyclophosphamide, and rituximab (FCR)-based chemoimmunotherapy in a homogeneous population with long follow-up (median 49.5 months). Addition of low-level positive MRD < 0.01% to MRD ≥ 0.01% increased the proportion of cases with positive MRD in blood by 39% and in bone marrow by 27%. Compared to low-level positive MRD < 0.01%, undetectable MRD was associated with significantly longer progression-free survival (PFS) when using blood (72.2 versus 42.7 months; hazard ratio 0.40, p = 0.0003), but not when using bone marrow. Upon further stratification, positive blood MRD at any level, compared to undetectable blood MRD, was associated with shorter PFS irrespective of clinical complete or partial remission, and a lower 5-year PFS rate irrespective of IGHV-mutated or -unmutated status (all p < 0.05). In conclusion, high-sensitivity (0.0007%) MRD assessment in blood yielded additional prognostic information beyond the current standard sensitivity (0.01%). Our approach provides a model for future determination of the optimal MRD investigative strategy for any regimen