Cropduster applying DDT to potato field (photo)

A large print found on top of a bookshelf in my office in the Plant Industry Building on East Campus, University of Nebraska–Lincoln. The office was previously occupied by Lloyd Anderson who was located at Scotts Bluff. Photo was taken in 1945 when the insecticide DDT dust was being applied to what appears to be a potato field. Steve Danielson, January 16, 201

EC07-1780 Lady Beetles of Nebraska

Extension Circular 07-1780 discusses lady beetles of Nebraska

Cross-Resistance and Resistance Longevity as Induced by Bean Leaf Beetle, Cerotoma trifurcata and Soybean Looper, Pseudoplusia includens herbivory on Soybean

Cross-resistance, and longevity of resistance, induced by the bean leaf beetle, Cerotoma trifurcata, was studied IN the soybean PI 227687 that exhibited induced response in earlier studies. Bean leaf beetle adults and soybean looper, Pseudoplusia includens, larvae were used to induce resistance and to determine beetle feeding preference. Beetles were collected from soybean fields 2 to 5 days prior to the feeding preference test. The level of cross-resistance induced by soybean looper herbivory to subsequent bean leaf beetle feeding was higher when compared to cross-resistance induced by bean leaf beetle herbivory against subsequent feeding by soybean looper. Further, herbivory by the bean leaf beetle also induced resistance against soybean looper feeding. In the longevity study, leaflets from treated plants were collected 5, 10, 12, 14, 16, 20 and 25 days after initiation of feeding. Pairwise comparisons of leaflets from plants treated by bean leaf beetle herbivory with untreated plants revealed that induced responses were highest 14 and lowest 25 days after initiation of feeding. On other sampling days, levels of induced response varied with the sampling day

G93-1145 Management of the Army Cutworm and Pale Western Cutworm

This NebGuide describes the life cycle of the army cutworm and pale western cutworm, and provides recommendations for management.The army cutworm, Euxoa auxiliaris, and the pale western cutworm, Agrotis orthogonia, are sporadic pests that are distributed throughout the Great Plains. The army cutworm can be found throughout Nebraska, but is more common in the western half of the state. Because of the drier environment, the pale western cutworm is found only in the western third of Nebraska. Both cutworms can feed on a vast array of crops and weeds. Their major economic impact is limited to winter wheat and alfalfa, because these are the vulnerable crops growing in the early spring when larval feeding activity occurs. However, they can also cause substantial damage to early spring row crops (sugarbeets and corn), especially in areas where winter cereal cover crops are used

Responses of predatory invertebrates to seeding density and plantspecies richness in experimental tallgrass prairie restorations

In recent decades, agricultural producers and non-governmental organizations have restored thousandsof hectares of former cropland in the central United States with native grasses and forbs. However,the ability of these grassland restorations to attract predatory invertebrates has not been well docu-mented, even though predators provide an important ecosystem service to agricultural producers bynaturally regulating herbivores. This study assessed the effects of plant richness and seeding density onthe richness and abundance of surface-dwelling (ants, ground beetles, and spiders) and aboveground(ladybird beetles) predatory invertebrates. In the spring of 2006, twenty-four 55 m × 55 m-plots wereplanted to six replicates in each of four treatments: high richness (97 species typically planted by TheNature Conservancy), at low and high seeding densities, and low richness (15 species representing a typ-ical Natural Resources Conservation Service Conservation Reserve Program mix, CP25), at low and highseeding densities. Ants, ground beetles, and spiders were sampled using pitfall traps and ladybird beetleswere sampled using sweep netting in 2007–2009. The abundance of ants, ground beetles, and spidersshowed no response to seed mix richness or seeding density but there was a significant positive effect ofrichness on ladybird beetle abundance. Seeding density had a significant positive effect on ground beetleand spider species richness and Shannon–Weaver diversity. These results may be related to differencesin the plant species composition and relative amount of grass basal cover among the treatments ratherthan richness

G93-1145 Management of the Army Cutworm and Pale Western Cutworm

This NebGuide describes the life cycle of the army cutworm and pale western cutworm, and provides recommendations for management.The army cutworm, Euxoa auxiliaris, and the pale western cutworm, Agrotis orthogonia, are sporadic pests that are distributed throughout the Great Plains. The army cutworm can be found throughout Nebraska, but is more common in the western half of the state. Because of the drier environment, the pale western cutworm is found only in the western third of Nebraska. Both cutworms can feed on a vast array of crops and weeds. Their major economic impact is limited to winter wheat and alfalfa, because these are the vulnerable crops growing in the early spring when larval feeding activity occurs. However, they can also cause substantial damage to early spring row crops (sugarbeets and corn), especially in areas where winter cereal cover crops are used

G91-1023 Insects That Attack Seeds and Seedlings of Field Crops

This NebGuide discusses how to identify and control eight insects that feed on planted seeds and seedlings. Planted seeds of field crops can attract several kinds of insects. In many cases, the insects do not cause enough damage to justify control procedures. However, under some conditions seed pests may be very destructive. Seed that is slow to germinate and establish is more likely to be damaged. Situations such as first-year crops following sod or pasture, ecofallow, or reduced tillage/heavy crop residue conditions have a good chance for seed/seedling damage. Consider grower experience and past pest problems when deciding about individual fields. This NebGuide describes eight insect pests which may attack seeds and seedlings of Nebraska field crops. Illustrations of insects are magnified to better show detail; an approximate level of magnification is indicated by the number in the caption

Clinical Safety-in-Use Study of a New Tampon Design

Objective: To confirm the safety of a new experimental Tampax(®) tampon and applicator compared with that of a currently marketed Tampax(®) tampon and applicator using comprehensive gynecological and microbiological assessments. Methods: A 2-month, single-blind, randomized, crossover study was conducted in which each subject served as her own control. Safety was evaluated by comparing potential product-related irritation (using colposcopic examination and subject diary data), assessment of vaginal discharge, vaginal pH, and effects on selected microorganisms (yeast, Escherichia coli ,Staphylococcus aureus and group B streptococci) obtained by vaginal swab cultures after normal menstrual use in the experimental and control groups. Results: In total, 110 women completed the study. There were no significant differences between the groups that used either the experimental or control tampon with regard to prevalence or mean cell density for the selected microorganisms. No differences were observed in the incidence or severity of erythema, in abrasion or ulceration of the cervix, vagina, introitus, vulva or perineum, or in mean vaginal pH and discharge assessments. There were equivalent low incidences of reported symptoms such as discomfort during insertion, wear or removal, and a similar low incidence of burning, stinging or itching during use of either the control or experimental tampon. There was a more favorable overall product rating for the experimental tampon (p = 0.003). Conclusions: This approach provides a combination of gynecological, microbiological and self-reported (diary recall) methodologies in order to assess tampon safety during use more thoroughly than has previously been reported, and it supports a comparable safety profile for the experimental tampon and a currently marketed tampon

Life-threatening influenza pneumonitis in a child with inherited IRF9 deficiency

Life-threatening pulmonary influenza can be caused by inborn errors of type I and III IFN immunity. We report a 5-yr-old child with severe pulmonary influenza at 2 yr. She is homozygous for a loss-of-function IRF9 allele. Her cells activate gamma-activated factor (GAF) STAT1 homodimers but not IFN-stimulated gene factor 3 (ISGF3) trimers (STAT1/STAT2/IRF9) in response to IFN-α2b. The transcriptome induced by IFN-α2b in the patient's cells is much narrower than that of control cells; however, induction of a subset of IFN-stimulated gene transcripts remains detectable. In vitro, the patient's cells do not control three respiratory viruses, influenza A virus (IAV), parainfluenza virus (PIV), and respiratory syncytial virus (RSV). These phenotypes are rescued by wild-type IRF9, whereas silencing IRF9 expression in control cells increases viral replication. However, the child has controlled various common viruses in vivo, including respiratory viruses other than IAV. Our findings show that human IRF9- and ISGF3-dependent type I and III IFN responsive pathways are essential for controlling IAV