Comprehensive analysis of the membrane phosphoproteome regulated by oligogalacturonides in Arabidopsis thaliana

Early changes in the Arabidopsis thaliana membrane phosphoproteome in response to oligogalacturonides (OGs), a class of plant damage-associated molecular patterns (DAMPs), were analyzed by two complementary proteomic approaches. Differentially phosphorylated sites were determined through phosphopeptide enrichment followed by LC-MS/MS using label-free quantification; differentially phosphorylated proteins were identified by 2D-DIGE combined with phospho-specific fluorescent staining (phospho-DIGE). This large-scale phosphoproteome analysis of early OG-signaling enabled us to determine 100 regulated phosphosites using LC-MS/MS and 46 differential spots corresponding to 34 pdhosphoproteins using phospho-DIGE. Functional classification showed that the OG-responsive phosphoproteins include kinases, phosphatases and receptor-like kinases, heat shock proteins (HSPs), reactive oxygen species (ROS) scavenging enzymes, proteins related to cellular trafficking, transport, defense and signaling as well as novel candidates for a role in immunity, for which elicitor-induced phosphorylation changes have not been shown before. A comparison with previously identified elicitor-regulated phosphosites shows only a very limited overlap, uncovering the immune-related regulation of 70 phosphorylation sites and revealing novel potential players in the regulation of elicitor-dependent immunity

Smoking Habits: Like Father, Like Son, Like Mother, Like Daughter

We use instrumental variable methods to investigate whether the impact of parental smoking habits on their children’s smoking decisions is a causal one. We find evidence of same-sex role models in two-parent households: mothers play a crucial role in determining their daughters’ smoking decisions, while fathers’ smoking habits are primarily imitated by their sons.youth smoking, intergenerational habit transmission, multivariate probit, instrumental variables

A humanidade em cada ser: O treinamento de ator/atriz com a máscara neutra.

TCC(graduação) - Universidade Federal de Santa Catarina. Centro de Comunicação e Expressão. TeatroEste trabalho propõe um olhar fenomenológico sobre o treino com a máscara neutra tendo como base teórica os ensinamentos de Jacques Copeau e Jacques Lecoq, argumentando com a prática vivenciada pela autora em diferentes momentos no decorrer do curso universitário de Artes Cênicas. Apresentando como sistema de treino o processo de colocar a máscara, vivenciar a máscara e tirar a máscara, a autora relata e avalia os exercícios aplicados assim como os resultados obtidos e as implicações destes no devir do individuo

High-resolution SAR images for fire susceptibility estimation in urban forestry

We present an adaptive system for the automatic assessment of both physical and anthropic fire impact factors on periurban forestries. The aim is to provide an integrated methodology exploiting a complex data structure built upon a multi resolution grid gathering historical land exploitation and meteorological data, records of human habits together with suitably segmented and interpreted high resolution X-SAR images, and several other information sources. The contribution of the model and its novelty rely mainly on the definition of a learning schema lifting different factors and aspects of fire causes, including physical, social and behavioural ones, to the design of a fire susceptibility map, of a specific urban forestry. The outcome is an integrated geospatial database providing an infrastructure that merges cartography, heterogeneous data and complex analysis, in so establishing a digital environment where users and tools are interactively connected in an efficient and flexible way

Diagnostic imaging and CEUS findings in a rare case of Desmoid-type fibromatosis. A case report

Desmoid-type fibromatosis (DF), also known as aggressive fibromatosis, is a locally aggressive benign fibroblastic neoplasm that can infiltrate or recur but cannot metastasize. It is rare, with an estimated annual incidence of two to four new cases per million people. Most DFs occur sporadically, but it may also be associated with the hereditary syndrome familial adenomatous polyposis. Treatment is necessary when the disease is symptomatic, especially in case of compression of critical structures. When possible, surgical resection is the treatment of choice; however, recurrence is common. Due to the high rate of recurrence, imaging plays an important role not only in diagnosis, but also in the management of DF. Although there are a number of studies describing CT and MRI findings of DF, there is no description of contrast-enhanced ultrasound findings

Coherent transport in extremely underdoped Nd1.2Ba1.8Cu3Oz nanostructures

Proximity-effect and resistance magneto-fluctuations measurements in submicron Nd1.2Ba1.8Cu3Oz (NBCO) nano-loops are reported to investigate coherent charge transport in the non-superconducting state. We find an unexpected inhibition of cooper pair transport, and a destruction of the induced superconductivity, by lowering the temperature from 6K to 250mK. This effect is accompanied by a significant change in the conductance-voltage characteristics and in the zero bias conductance response to the magnetic field pointing to the activation of a strong pair breaking mechanism at lower temperature. The data are discussed in the framework of mesoscopic effects specific to superconducting nanostructures, proximity effect and high temperature superconductivity.Comment: to appear on new journal of Physic

Evaluation of the Effects of Anxiety on Behaviour and Physiological Parameters in VR Fire Simulations

This study considers the use of virtual reality technologies both to evaluate the optimal exodus paths from buildings and to analyze the behaviour of people in emergencies through the monitoring of physiological parameters. The degree of realism with which VR devices can simulate highly anxiogenic scenarios is considered. The experimentation is carried out on a school building and tested through a pilot trial where saturation, blood pressure, heart rate as well as the time of exodus are monitored. The variations of the peak values of physiological parameters highlight the influence of anxiogenic elements in the participant's state of anxiety

A new construct to measure participation on social networking sites

Langaro, D., Salgueiro, M. D. F., & Rita, P. (2020). Users' brand page participation: A new construct to measure participation on social networking sites. International Journal of Internet Marketing and Advertising, 14(2), 135-151. https://doi.org/10.1504/IJIMA.2020.107658The increasing development of brand presence on social networking sites (SNS) requires new practices for monitoring the results of brands' efforts. The current research proposes that users' exposure to brand-related content by means of participating on brand pages is a key measure to be monitored. This proposition builds on previous studies which relate positive business consequences with users' exposure to brand communications. Despite the relevance of capturing users' participation on brand pages, so far very limited effort has been made to propose a construct for measuring it. Thus, three studies were implemented and a new construct was proposed and validated for internal consistency, unidimensionality and nomological validity.authorsversionpublishe

Biohydrogen production from food waste: Influence of the inoculum-to-substrate ratio

In this study, the influence of the inoculum-to-substrate ratio (ISR) on dark fermentative hydrogen production from food waste (FW) was evaluated. ISR values ranging from 0.05 to 0.25 g VSinoculum/g VSsubstrate were investigated by performing batch tests at T = 39 °C and pH = 6.5, the latter being the optimal value identified based on a previous study. The ISR was found to affect the fermentation process, clearly showing that an adequate ISR is essential in order to optimise the process kinetics and the H2 yield. An ISR of 0.14 proved to optimum, leading to a maximum H2 yield of 88.8 L H2/kg VSFW and a maximum production rate of 10.8 L H2/kg VSFW∙h. The analysis of the fermentation products indicated that the observed highest H2 production mostly derived from the typical acetate/butyrate-type fermentation

Understanding the Effects of Lactose Hydrolysis Modeling on the Main Oligosaccharides in Goat Milk Whey Permeate.

Enzymatic hydrolysis of lactose is a crucial step to improve the efficiency and selectivity of membrane-based separations toward the recovery of milk oligosaccharides free from simple sugars. Response surface methodology was used to investigate the effects temperature (25.9 to 54.1 °C) and amount of enzyme (0.17 to 0.32% w/w) at 1, 2, and 4 h of reaction on the efficiency of lactose hydrolysis by Aspergillus oryzae β-galactosidase, preservation of major goat whey oligosaccharides, and on the de-novo formation of oligosaccharides. Lactose hydrolysis above 99% was achieved at 1, 2, and 4 h, not being significantly affected by temperature and amount of enzyme within the tested conditions. Formation of 4 Hexose (Hex) and 4 Hex 1 Hex and an increased de-novo formation of 2 Hex 1 N-Acetyl-Neuraminic Acid (NeuAc) and 2 Hex 1 N-Glycolylneuraminic acid (NeuGc) was observed in all treatments. Overall, processing conditions using temperatures ≤40 °C and enzyme concentration ≤0.25% resulted in higher preservation/formation of goat whey oligosaccharides