Ouabain-insensitive, Na-ATPase activity in pure suspensions of rat kidney proximal tubules

AbstractThe present work was undertaken to evaluate the distribution of the Na-ATPase activity in the different components of the rat kidney cortex. Suspensions of glomeruli, proximal and distal tubules were prepared following a collagenase digestion of outermost kidney cortex slices and a separation on a Percoll gradient. It was found that the Na-ATPase activity is higher in the fraction enriched in proximal tubules. The fraction enriched in glomeruli and in distal tubules show also a Na-ATPase activity, but it is lower

Biotechnological Approach for the Production of Prebiotics and Search for New Probiotics and their Application in the Food Industry

Background and objective: Prebiotics and probiotics intake have been widely recognized in past recent years due to possessing multiple health benefits. Prebiotics are non-digestible carbohydrates that promote the growth and/or activity of beneficial bacteria in the colon which improves the health. Moreover, the incorporation of probiotics in food has also been a growing practice due to its immunomodulatory effect, the production of organic acids and other compounds that promotes the absorption of nutrients and the general health of the digestive system.Results and conclusion: Biotechnological strategies have been proposed for prebiotic production and purification in order to meet the demand to be included as ingredients in functional food formulation. Different aspects related to the substrates and different fermentation systems for their production as well as the purification and characterization processes are addressed. Also, we will present the benefits promoted by probiotics, the methods of isolation and characterization, as well as the evaluation of these attributes, so that they can be used in the food industry. With the technological developments in prebiotics and probiotics, it will be possible to deliver foods that respond to consumer demand with low cost and with pleasant sensory characteristics as well as providing beneficial health effects.Conflict of interest: The authors declare no conflict of interest

Highlights of the 11th International Bordetella Symposium: From basic biology to vaccine development

Pertussis is a severe respiratory disease caused by infection with the bacterial pathogen Bordetella pertussis. The disease affects individuals of all ages but is particularly severe and sometimes fatal in unvaccinated young infants. Other Bordetella species cause diseases in humans, animals, and birds. Scientific, clinical, public health, vaccine company, and regulatory agency experts on these pathogens and diseases gathered in Buenos Aires, Argentina from 5 to 8 April 2016 for the 11th International Bordetella Symposium to discuss recent advances in our understanding of the biology of these organisms, the diseases they cause, and the development of new vaccines and other strategies to prevent these diseases. Highlights of the meeting included pertussis epidemiology in developing nations, genomic analysis of Bordetella biology and evolution, regulation of virulence factor expression, new model systems to study Bordetella biology and disease, effects of different vaccines on immune responses, maternal immunization as a strategy to prevent newborn disease, and novel vaccine development for pertussis. In addition, the group approved the formation of an International Bordetella Society to promote research and information exchange on bordetellae and to organize future meetings. A new Bordetella.org website will also be developed to facilitate these goals.Instituto de Biotecnologia y Biologia Molecula

Highlights of the 11th International Bordetella Symposium: From basic biology to vaccine development

Pertussis is a severe respiratory disease caused by infection with the bacterial pathogen Bordetella pertussis. The disease affects individuals of all ages but is particularly severe and sometimes fatal in unvaccinated young infants. Other Bordetella species cause diseases in humans, animals, and birds. Scientific, clinical, public health, vaccine company, and regulatory agency experts on these pathogens and diseases gathered in Buenos Aires, Argentina from 5 to 8 April 2016 for the 11th International Bordetella Symposium to discuss recent advances in our understanding of the biology of these organisms, the diseases they cause, and the development of new vaccines and other strategies to prevent these diseases. Highlights of the meeting included pertussis epidemiology in developing nations, genomic analysis of Bordetella biology and evolution, regulation of virulence factor expression, new model systems to study Bordetella biology and disease, effects of different vaccines on immune responses, maternal immunization as a strategy to prevent newborn disease, and novel vaccine development for pertussis. In addition, the group approved the formation of an International Bordetella Society to promote research and information exchange on bordetellae and to organize future meetings. A new Bordetella.org website will also be developed to facilitate these goals.Instituto de Biotecnologia y Biologia Molecula

Desarrollo de estrategias para comercialización y comunicación digital con el fin de dar a conocer los productos y nuevo servicio que ofrece Vivero La Pradera, en la ZMG durante el periodo de agosto a diciembre de 2022

ITESO, A.C

Emergence of ceftazidime/avibactam resistance in KPC-8–producing Klebsiella pneumoniae in South America

Klebsiella pneumoniae is one of the so-called ESKAPE pathogens. These organisms are the main cause of nosocomial infections worldwide, causing life-threatening infections amongst critically ill and immunocompromised individuals. They are characterized by drug resistance mechanisms. Klebsiella pneumoniae carbapenemase (KPC)-producing isolates display resistance to multiple antimicrobial agents, usually including last-resort alternative options, leading to an urgent need to develop new drugs or combinations. In Argentina sequence type (ST) 258 harbouring bla KPC-2 emerged in 2010 and remained prevalent until the last few years, when the emergence of different STs such as ST25, ST11 and ST307 appeared likely to change the local epidemiology.Fil: García, J.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Nastro, Marcela. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Cejas, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Instituto de Investigaciones En Bacteriologia y Virologia Molecular; ArgentinaFil: Santana, G.. No especifíca;Fil: Mancino, M.B.. No especifíca;Fil: Hidalgo, M.. No especifíca;Fil: Maccallini, G.. No especifíca;Fil: Vay, C.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Radice, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Instituto de Investigaciones En Bacteriologia y Virologia Molecular; ArgentinaFil: Dabos, L.. Université Paris Sud; FranciaFil: Famiglietti, Angela María Rosa. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Rodríguez, H.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentin

Rapid Detection of Foodborne ESBL-Producing Enterobacteriaceae Using MALDI-TOF Mass Spectrometry

El artículo es una ponencia presentada en Presented at the 3rd International Electronic Conference on Antibiotics (ECA 2023), 1–15 December 2023; Available online: https://eca2023.sciforum.net/.[EN] The proliferation of enterobacteria that produce extended spectrum β-lactamases (ESBLs) has become an important public health concern. The objective of this work was to optimize a phenotypic method for straightforward and rapid ESBL detection in strains obtained from food environments, employing MALDI-TOF mass spectrometry. A collection of 162 enterobacterial isolates, including ESBL producers, AmpC producers, ESBL-AmpC coproducers and sensitive strains was analyzed using a quick method based on cefotaxime hydrolysis. Statistical analysis showed that although the presence of constitutive AmpC hyperproducers could reduce its specificity, this MALDI-TOF assay is an excellent tool for quickly classifying ESBL-producing strains of foodborne Enterobacteriaceae.SIThis research was supported by “Ministerio de Ciencia e Innovación—Agencia Estatal de Investigación/10.13039/501100011033”, Project PID2019-107870RB-I00. A. Pintor-Cora benefits from a predoctoral contract from the Universidad de León

MXene-containing composite electrodes for hydrogen evolution: material design aspects and approaches for electrode fabrication

This work explores the possibilities for the processing of Ni- and Ti3C2Tx (T = OH, O) MXene-containing composite electrodes, by co-pressing and plastic deformation or by etching of the electrodes prepared directly by self-propagation high-temperature synthesis (SHS). Various material design approaches were also explored. In order to tune the Ti3C2 interlayer distance in Ti3C2Al MAX phase, an introduction of additional Al to form Ti3C2Alz materials with z > 1 was attempted. Self-propagation high-temperature synthesis of powder mixtures with extra Ni and Al content (e.g. Ni:Ti:Al:C = 1:2:3:1) resulted in SHS products containing Ti3C2Alz z > 1 material and Ni–Al alloys. Further etching of these products in 10M NaOH allowed the direct formation of electrodes with active surface containing Ti3C2Tx (T = OH, O) MXene- and Raney nickel-containing composites. The electrochemical studies were focused on hydrogen evolution and showed the potential for boosting the electrochemical reaction in Ni and MXene-containing composite electrodes, especially at high current densities. The guidelines for the processing of such electrodes under fluorine-free conditions are proposed and discussed.publishe

Validar nuevo empaque para Maicitos MX considerando estrategias y contenido adecuado para su comercialización en la ZMG

ITESO, A.C

Women in Science: A Student Workshop at a University in Mexico

Women have participated in science since the earliest of times. However, their valuable contributions are not as widely acknowledged throughout our history and women still face several barriers throughout their professional career. We developed and hosted a half-day Women in Science seminar organized by students, for students. The main goal was to promote ways to involve more women in research activities and to strengthen the scientific community within our medical school. The workshop consisted of three main sections, where we shared relevant statistics, resources, and strategies among a community of students interested in science. At the end of the workshop, participants shared their insights proving the necessity and utility of such events to strengthen the development of science as an inclusive field