The Neural Encoding of Cocaine-Induced Devaluation in The Ventral Pallidum

Cocaine experience affects motivation structures such as the nucleus accumbens (NAc) and its major output target, the ventral pallidum (VP). Previous studies demonstrated that both NAc activity and hedonic responses change reliably as a taste cue comes to predict cocaine availability. Here we extended this investigation to examine drug-experience induced changes in hedonic encoding in the VP. VP activity was first characterized in adult male Sprague–Dawley rats in response to intraoral infusions of palatable saccharin and unpalatable quinine solutions. Next, rats received 7 daily pairings of saccharin that predicted either a cocaine (20 mg/kg, ip) or saline injection. Finally, the responses to saccharin and quinine were again assessed. Of 109 units recorded in 11 rats that received saccharin–cocaine pairings, 71% of responsive units significantly reduced firing rate during saccharin infusions and 64% increased firing rate during quinine exposure. However, as saccharin came to predict cocaine, and elicited aversive taste reactivity, VP responses changed to resemble quinine. After conditioning, 70% of saccharin-responsive units increased firing rate. Most units that encoded the palatable taste (predominantly reduced firing rate) were located in the anterior VP, while most units that were responsive to aversive tastes were located in the posterior VP. This study reveals an anatomical complexity to the nature of hedonic encoding in the VP

The Role of the Magnetorotational Instability in the Sun

We calculate growth rates for nonaxisymmetric instabilities including the magnetorotational instability (MRI) throughout the Sun. We first derive a dispersion relation for nonaxisymmetric instability including the effects of shear, convective buoyancy, and three diffusivities (thermal conductivity, resistivity, and viscosity). We then use a solar model evolved with the stellar evolution code MESA and angular velocity profiles determined by Global Oscillations Network Group (GONG) helioseismology to determine the unstable modes present at each location in the Sun and the associated growth rates. The overall instability has unstable modes throughout the convection zone and also slightly below it at middle and high latitudes. It contains three classes of modes: large-scale hydrodynamic convective modes, large-scale hydrodynamic shear modes, and small-scale magnetohydrodynamic (MHD) shear modes, which may be properly called MRI modes. While large-scale convective modes are the most rapidly growing modes in most of the convective zone, MRI modes are important in both stably stratified and convectively unstable locations near the tachocline at colatitudes theta less than 53 degrees. Nonaxisymmetric MRI modes grow faster than the corresponding axisymmetric modes; for some poloidal magnetic fields, the nonaxisymmetric MRI growth rates are similar to the angular rotation frequency Omega, while axisymmetric modes are stabilized. We briefly discuss the saturation of the field produced by MRI modes, finding that the implied field at the base of the convective zone in the Sun is comparable to that derived based on dynamos active in the tachocline and that the saturation of field resulting from the MRI may be of importance even in the upper convection zone.Comment: 20 pages, 11 figure

Drug Predictive Cues Activate Aversion-Sensitive Striatal Neurons That Encode Drug Seeking

Drug-associated cues have profound effects on an addict’s emotional state and drug-seeking behavior. Although this influence must involve the motivational neural system that initiates and encodes the drug-seeking act, surprisingly little is known about the nature of such physiological events and their motivational consequences. Three experiments investigated the effect of a cocaine-predictive stimulus on dopamine signaling, neuronal activity, and reinstatement of cocaine seeking. In all experiments, rats were divided into two groups (paired and unpaired), and trained to self-administer cocaine in the presence of a tone that signaled the immediate availability of the drug. For rats in the paired group, self-administration sessions were preceded by a taste cue that signaled delayed drug availability. Assessments of hedonic responses indicated that this delay cue became aversive during training. Both the self-administration behavior and the immediate cue were subsequently extinguished in the absence of cocaine. After extinction of self-administration behavior, the presentation of the aversive delay cue reinstated drug seeking. In vivo electrophysiology and voltammetry recordings in the nucleus accumbens measured the neural responses to both the delay and immediate drug cues after extinction. Interestingly, the presentation of the delay cue simultaneously decreased dopamine signaling and increased excitatory encoding of the immediate cue. Most importantly, the delay cue selectively enhanced the baseline activity of neurons that would later encode drug seeking. Together these observations reveal how cocaine cues can modulate not only affective state, but also the neurochemical and downstream neurophysiological environment of striatal circuits in a manner that promotes drug seeking

Kids Count Alaska 2013-2014

This research was funded by the Annie E. Casey Foundation. We thank the foundation for its support, but the findings and conclusions in this report are those of the authors and not necessarily of the foundation. KIDS COUNT is a nationwide program of the Annie E. Casey Foundation. The foundation produces a national data book each year, detailing the condition of America’s children. It also sponsors KIDS COUNT programs in all 50 states. Feel free to copy, distribute, or otherwise use information from the Kids Count Alaska Data Book, citing the source as: Kids Count Alaska 2013-2014 Data Book, prepared by the Institute of Social and Economic Research, University of Alaska Anchorage, with funding from the Annie E. Casey Foundation.Kids Count Alaska is part of a nationwide program, sponsored by the Annie E. Casey Foundation, to collect and publicize information about children’s health, safety, education, and economic status. We gather information from many sources and present it in one place, to give Alaskans and others a broad picture of how well the state’s children are doing—and provide parents, policymakers, and others with information they need to improve life for children and families. Our goals are: • Distributing information about the status of Alaska’s children • Creating an informed public, motivated to help children • Comparing the status of children in Alaska with that of children nationwide, but also presenting additional indicators relevant for AlaskaAnnie E. Casey FoundationIntroduction / Infancy / Births and Care Before Birth / Babies With Low Birthweight / Infant Mortality / Economic Well-Being / Children Living in Poverty / Children With No Parent Working Full-Time, Year-Round / Children in Single-Parent Families / Births to Teens / Health Care / Education / Dropout and Graduation Rates / Teens Not in School and Not Working / School Achievement / Children in Danger / Child Death Rate / Teen Death Rate / Child Abuse and Neglect / Juvenile Justic

Modeling Self-Referencing Interferometers with Extended Beacons and Strong Turbulence

The overall purpose of this research was to better understand the performance of a self-referencing interferometer (SRI) when used with extended beacons in strong atmospheric turbulence. It was performed by assuming the extended beacon could be modeled as a Gaussian Schell-model beam, then analyzing the effect of propagating this beam through strong atmospheric turbulence. Since the operation of an SRI requires coupling this light into a single-mode optical fiber, analytic expressions of the mean and normalized variance of the coupling efficiency were derived. An improved noise model for the SRI was then developed that included all potential noise sources such as intensity fluctuations of the incident light, the single-mode fiber coupling efficiency, the spatial and temporal coherence properties of the light, and other additive noise sources. Whenever simplifying assumptions were used, the results were compared to numerically evaluated exact expressions or Monte Carlo simulations. Any resulting error was identified then typically compensated

Perspectives of change: Municipal park and recreation directors perceptions of the importance of specified organizational goals 1983–2008

This study examines specified goals park and recreation directors have previously determined are important for their organizations, and how those goals rank in importance in their present estimations, and how those goals rank in future importance. It compares a similar study of goals from 1983, and reports on changes in levels of importance from 1983 to 2008. Directors\u27 perceptions of goal importance of 2008 present levels and 2008 future levels were determined to be significantly different in 23 of 26 cases. Goals were grouped in four categories, (a) management/adaptation,/positional, having to do with management tasks, (b) groups served, those targeted populations, (c) services provided, services of varying kinds provided to other groups, organizations, and individuals, and (d) desired outcomes, those benefits or changes occurring as a result of participation. A factor analysis was performed which confirmed that grouping of goals in these four categories remains relevant. Goals were grouped and ranked in each of the four categories, and then ranked overall. Directors\u27 perceptions of 2008 future levels of goal importance increased significantly over 2008 present levels in 23 of 26 cases. Changes in rank ordering of goals indicate potential shifts in priorities and attendant shifts in allocation of resources. A comparison of 2008 present goals with 1983 future goals was performed. Significant changes in rank ordering of specific goals were discovered in 16 of 26 goals. Park and recreation directors\u27 perceptions from 1983 to 2008 have moved from organizational concerns to concern for service to specific targeted groups. Children ranked as the top priority, with teens, seniors and adults all in the top ten rankings in 2008. Special needs populations and management activities goals fell in the rankings, as did programs for people with special needs. Programs for ethnic and cultural minorities rose slightly, but remained in the bottom ten. A discussion of implications of these perceived priorities among park and recreation directors was conducted, with consideration of both survival and expansion of recreation programs considered. Recommendations for further study and an outline of a study agenda was made to improve the quality and relevance of goals studies overall

Aversive Stimuli Drive Drug Seeking in a State of Low Dopamine Tone

Background Stressors negatively impact emotional state and drive drug seeking, in part, by modulating the activity of the mesolimbic dopamine system. Unfortunately, the rapid regulation of dopamine signaling by the aversive stimuli that cause drug seeking is not well characterized. In a series of experiments, we scrutinized the subsecond regulation of dopamine signaling by the aversive stimulus, quinine, and tested its ability to cause cocaine seeking. Additionally, we examined the midbrain regulation of both dopamine signaling and cocaine seeking by the stress-sensitive peptide, corticotropin releasing factor (CRF). Methods Combining fast-scan cyclic voltammetry with behavioral pharmacology, we examined the effect of intraoral quinine administration on nucleus accumbens dopamine signaling and hedonic expression in 21 male Sprague-Dawley rats. We tested the role of CRF in modulating aversion-induced changes in dopamine concentration and cocaine seeking by bilaterally infusing the CRF antagonist, CP-376395, into the ventral tegmental area (VTA). Results We found that quinine rapidly reduced dopamine signaling on two distinct time scales. We determined that CRF acted in the VTA to mediate this reduction on only one of these time scales. Further, we found that the reduction of dopamine tone and quinine-induced cocaine seeking were eliminated by blocking the actions of CRF in the VTA during the experience of the aversive stimulus. Conclusions These data demonstrate that stress-induced drug seeking can occur in a terminal environment of low dopamine tone that is dependent on a CRF-induced decrease in midbrain dopamine activity

New Chair Alliance

The New Chair Alliance is the conference’s special programming for newly appointed chairpersons. Those who will soon transition into a chair position or who have been a chair for two years or less will find this workshop particularly valuable. Topics covered include transitioning to new roles and responsibilities, annual planning, everyday management, strategic planning for the future, personal development, and finding satisfaction in being a chair and making a difference. Participants will gain practical tips and tools to improve effectiveness, become familiar with essential resources, learn how to identify an administrative mentor, as well as connect and network with others at a similar career stage

Optogenetic Interrogation of Functional Synapse Formation by Corticospinal Tract Axons in the Injured Spinal Cord

To restore function after injury to the CNS, axons must be stimulated to extend into denervated territory and, critically, must form functional synapses with appropriate targets. We showed previously that forced overexpression of the transcription factor Sox11 increases axon growth by corticospinal tract (CST) neurons after spinal injury. However, behavioral outcomes were not improved, raising the question of whether the newly sprouted axons are able to form functional synapses. Here we developed an optogenetic strategy, paired with single-unit extracellular recordings, to assess the ability of Sox11-stimulated CST axons to functionally integrate in the circuitry of the cervical spinal cord. Initial time course experiments established the expression and function of virally expressed Channelrhodopsin (ChR2) in CST cell bodies and in axon terminals in cervical spinal cord. Pyramidotomies were performed in adult mice to deprive the left side of the spinal cord of CST input, and the right CST was treated with adeno-associated virus (AAV)–Sox11 or AAV–EBFP control, along with AAV–ChR2. As expected, Sox11 treatment caused robust midline crossing of CST axons into previously denervated left spinal cord. Clear postsynaptic responses resulted from optogenetic activation of CST terminals, demonstrating the ability of Sox11-stimulated axons to form functional synapses. Mapping of the distribution of CST-evoked spinal activity revealed overall similarity between intact and newly innervated spinal tissue. These data demonstrate the formation of functional synapses by Sox11-stimulated CST axons without significant behavioral benefit, suggesting that new synapses may be mistargeted or otherwise impaired in the ability to coordinate functional output. SIGNIFICANCE STATEMENT As continued progress is made in promoting the regeneration of CNS axons, questions of synaptic integration are increasingly prominent. Demonstrating direct synaptic integration by regenerated axons and distinguishing its function from indirect relay circuits and target field plasticity have presented technical challenges. Here we force the overexpression of Sox11 to stimulate the growth of corticospinal tract axons in the cervical spinal cord and then use specific optogenetic activation to assess their ability to directly drive postsynaptic activity in spinal cord neurons. By confirming successful synaptic integration, these data illustrate a novel optogenetic-based strategy to monitor and optimize functional reconnection by newly sprouted axons in the injured CNS