Use of potentially abusive psychotropic substances in psychiatric inpatients

A series of 417 consecutively admitted psychiatric inpatients were studied with regard to their use of potentially abusive psychotropic substances in the last 3 months preceding admission. In all patients face-to-face interviews were performed; in 354 of them urine specimens could also be tested. Alcohol and benzodiazepines belonged to the most frequently used substances followed by cannabis, opiates and cocaine. Barbiturates, hallucinogens and amphetamine derivatives were only exceptionally reported. The most important finding of the study is that every fifth patient regularly used "hard” drugs (opiates and/or cocaine), every fourth patient illegal drugs and every third patient alcohol. Substances were found in 54% of all urine specimens; methadone, opiates and cocaine were hardly found alone. For the latter substances excellent agreement was found between interview reports and urine exams. Excluding patients diagnosed as substanceuse disorders, there were no statistically significant differences between schizophrenic, affective, neurotic/stress/ somatoform and other disorders with regard to the use of "hard” drugs and illegal drugs. Regular substance use correlated with much worse psychosocial adjustment. Substance use has to be explored and considered in every individual psychiatric inpatien

Two-color STED microscopy reveals different degrees of colocalization between hexokinase-I and the three human VDAC isoforms

The voltage-dependent anion channel (VDAC, also known as mitochondrial porin) is the major transport channel mediating the transport of metabolites, including ATP, across the mitochondrial outer membrane. Biochemical data demonstrate the binding of the cytosolic protein hexokinase-I to VDAC, facilitating the direct access of hexokinase-I to the transported ATP. In human cells, three hVDAC isoforms have been identified. However, little is known on the distribution of these isoforms within the outer membrane of mitochondria and to what extent they colocalize with hexokinase-I. In this study we show that whereas hVDAC1 and hVDAC2 are localized predominantly within the same distinct domains in the outer membrane, hVDAC3 is mostly uniformly distributed over the surface of the mitochondrion. We used two-color stimulated emission depletion (STED) microscopy enabling a lateral resolution of ~40 nm to determine the detailed sub-mitochondrial distribution of the three hVDAC isoforms and hexokinase-I. Individual hVDAC and hexokinase-I clusters could thus be resolved which were concealed in the confocal images. Quantitative colocalization analysis of two-color STED images demonstrates that within the attained resolution, hexokinase-I and hVDAC3 exhibit a higher degree of colocalization than hexokinase-I with either hVDAC1 or hVDAC2. Furthermore, a substantial fraction of the mitochondria-bound hexokinase-I pool does not colocalize with any of the three hVDAC isoforms, suggesting a more complex interplay of these proteins than previously anticipated. This study demonstrates that two-color STED microscopy in conjunction with quantitative colocalization analysis is a powerful tool to study the complex distribution of membrane proteins in organelles such as mitochondria

A deviant EEG brain microstate in acute, neuroleptic-naive schizophrenics at rest

Momentary brain electric field configurations are manifestations of momentary global functional states of the brain. Field configurations tend to persist over some time in the sub-second range (“microstates”) and concentrate within few classes of configurations. Accordingly, brain field data can be reduced efficiently into sequences of re-occurring classes of brain microstates, not overlapping in time. Different configurations must have been caused by different active neural ensembles, and thus different microstates assumedly implement different functions. The question arises whether the aberrant schizophrenic mentation is associated with specific changes in the repertory of microstates. Continuous sequences of brain electric field maps (multichannel EEG resting data) from 9 neuroleptic-naive, first-episode, acute schizophrenics and from 18 matched controls were analyzed. The map series were assigned to four individual microstate classes; these were tested for differences between groups. One microstate class displayed significantly different field configurations and shorter durations in patients than controls; degree of shortening correlated with severity of paranoid symptomatology. The three other microstate classes showed no group differences related to psychopathology. Schizophrenic thinking apparently is not a continuous bias in brain functions, but consists of intermittent occurrences of inappropriate brain microstates that open access to inadequate processing strategies and context informatio

AutomAl 6000: Semi-automatic structural labelling of HAADF-STEM images of precipitates in Al–Mg–Si(–Cu) alloys

When the Al–Mg–Si(–Cu) alloy system is subjected to age hardening, different types of precipitates nucleate depending on the composition and thermomechanical treatment. The main hardening precipitates extend as needles, laths or rods along the directions in the aluminium matrix. It has been found that the structures of all metastable precipitates may be generalized as stacks of columns, where most of these columns are replaced by solute elements. In the precipitates, a column relates to neighbour columns by a set of simple structural principles, which allows identification of species and relative longitudinal displacement over the (100) cross-section. Aberration-corrected high-angle annular dark field scanning transmission electron microscopy (HAADF-STEM) is an important tool for studying such precipitates. With the goal of analysing atomic resolution HAADF-STEM images of precipitate cross-sections in the Al–Mg–Si(–Cu) system, we have developed the stand-alone software AutomAl 6000, which features a column characterization algorithm based on the symbiosis of a statistical model and the structural principles formulated in a digraph-like framework. The software can semi-autonomously determine the 3D column positions in the image, as well as column species. In turn, AutomAl 6000 can then display, analyse and/or export the structure data. This paper describes the methodology of AutomAl 6000 and applies it on three different HAADF-STEM images, which demonstrate the methodology. The software, as well as other resources, are available at http://automal.org. The source code is also directly available from https://github.com/Haawk666/AutomAl-6000.publishedVersio

On the Number of Pentagons in Triangle-Free Graphs

Using the formalism of flag algebras, we prove that every triangle-free graph $G$ with $n$ vertices contains at most $(n/5)^5$ cycles of length five. Moreover, the equality is attained only when $n$ is divisible by five and $G$ is the balanced blow-up of the pentagon. We also compute the maximal number of pentagons and characterize extremal graphs in the non-divisible case provided $n$ is sufficiently large. This settles a conjecture made by Erd\H{o}s in 1984.Comment: 16 pages, accepted to Journal of Combinatorial Theory Ser.

Dynamic interaction in tropical Africa: IGCP-616Y and IGCP 646 projects and events

The scientific objectives and research program of the IGCP-646 project (2015–2018) cuts across many disciplines and includes various aspects of continental basement geology, resource exploration (mineral, water and hydrocarbons), geohazard mitigation, and climate change, all of which are of critical importance to developing countries, particularly in parts of West Africa where population pressures are on the rise. Considerable emphasis was placed on capacity building, creation of opportunities for young scientists to undertake higher degrees programs, knowledge transfer and training. The SIDA-funded “pilot project” IGCP-616Y (started in 2012), focused on three objects (i) crustal architecture, tectonic evolution and regional geology of Central Africa and the connection with NE Brazil; (ii) the Mesozoic continental rifting and breakup leading to a better integration of the onshore and offshore geology; (iii) clarification and quantification of the links between basement structures, neotectonics, climate change and landscape evolution. The IGCP-616Y and IGCP-646 projects consisted of over 250 researchers, from different countries. In the course of the projects, six annual meetings, four field trips/workshops, as well as several training sessions were organized. Here we provide a summary of the scientific targets of the projects and a summary of the organized activities

High-Resolution Laboratory Measurements and Identification of Fe IX Lines near 171 Å

A multitude of weaker Fe IX lines have been predicted in the vicinity of the strong 171 Å line that dominates the spectra of many astrophysical and laboratory sources. Some of these weaker lines have only recently been identified in the laboratory, albeit some only tentatively. Here, we present measurements on the Livermore EBIT-I electron beam ion trap that span the region from 170.0 to 173.6 Å, which surrounds the 171 Å line. The measurements stepped through electron beam energy to determine the charge state of iron associated with each observed feature. Moreover, we have minimized the presence of oxygen in the trap, because oxygen lines obscured possible Fe IX lines in past measurements and prevented their identification. Our measurement confirms formerly tentative identifications and adds several new assignments