5 research outputs found
Dual Sensor for Cd(II) and Ca(II): Selective Nanoliter-Scale Sensing of Metal Ions
The
first selective, dual sensor for Ca<sup>2+</sup> and Cd<sup>2+</sup> capable of detection at 100 pM concentrations was designed
and synthesized. The experimental observations made for the MC-cation
complexes and the selectivity of compounds <b>1</b> and <b>2</b> with Ca<sup>2+</sup> and Cd<sup>2+</sup> ions were further
explored using density functional theory. A first step toward a nanoliter-scale
dip sensor for the dual sensing of Ca<sup>2+</sup> and Cd<sup>2+</sup> was demonstrated using microstructured optical fiber as the sensing
platform which is important for ion sensing in confined spaces such
as the medium surrounding cell clusters. In addition, this system
displays picomolar sensitivity for these ions, with an added ability
to reproducibly turn ion-binding on/off
Microstructured Optical Fibers and Live Cells: A Water-Soluble, Photochromic Zinc Sensor
A new
biologically compatible Zn(II) sensor was fabricated by embedding
a Zn(II) sensing spiropyran within the surface of a liposome derived
from Escherichia coli lipids (<b>LSP2</b>). Solution-based experiments with increasing Zn(II) concentrations
show improved aqueous solubility and sensitivity compared to the isolated
spiropyran molecule (<b>SP2</b>). <b>LSP2</b> is capable
of sensing Zn(II) efflux from dying cells with preliminary data indicating
that sensing is localized near the surface membrane of HEK 293 cells.
Finally, <b>LSP2</b> is suitable for development into a nanoliter-scale
dip-sensor for Zn(II) using microstructured optical fiber as the sensing
platform to detect Zn(II) in the range of 100 ρM with minimal
photobleaching. Existing spiropyran based sensing molecules can thus
be made biologically compatible, with an ability to operate with improved
sensitivity using nanoscale liquid sample volumes. This work represents
the first instance where photochromic spiropyran molecules and liposomes
are combined to create a new and multifunctional sensing entity for
Zn(II)
Microstructured optical fibers and live cells: a water-soluble, photochromic zinc sensor
A new biologically compatible Zn(II) sensor was fabricated by embedding a Zn(II) sensing spiropyran within the surface of a liposome derived from Escherichia coli lipids (LSP2). Solution-based experiments with increasing Zn(II) concentrations show improved aqueous solubility and sensitivity compared to the isolated spiropyran molecule (SP2). LSP2 is capable of sensing Zn(II) efflux from dying cells with preliminary data indicating that sensing is localized near the surface membrane of HEK 293 cells. Finally, LSP2 is suitable for development into a nanoliter-scale dip-sensor for Zn(II) using microstructured optical fiber as the sensing platform to detect Zn(II) in the range of 100 ρM with minimal photobleaching. Existing spiropyran based sensing molecules can thus be made biologically compatible, with an ability to operate with improved sensitivity using nanoscale liquid sample volumes. This work represents the first instance where photochromic spiropyran molecules and liposomes are combined to create a new and multifunctional sensing entity for Zn(II).Sabrina Heng, Christopher A. McDevitt, Andrew D. Abell, Daniel B Stubing, Jonathan J. Whittall, Jeremy G. Thompson, Timothy K Engler, Tanya M. Monr
Dual sensor for Cd(II) and Ca(II): selective nanoliter-scale sensing of metal ions
The first selective, dual sensor for Ca²⁺ and Cd²⁺ capable of detection at 100 pM concentrations was designed and synthesized. The experimental observations made for the MC-cation complexes and the selectivity of compounds 1 and 2 with Ca²⁺ and Cd²⁺ ions were further explored using density functional theory. A first step toward a nanoliter-scale dip sensor for the dual sensing of Ca²⁺ and Cd²⁺ was demonstrated using microstructured optical fiber as the sensing platform which is important for ion sensing in confined spaces such as the medium surrounding cell clusters. In addition, this system displays picomolar sensitivity for these ions, with an added ability to reproducibly turn ion-binding on/off.Sabrina Heng, Adrian M. Mak, Daniel B. Stubing, Tanya M. Monro, and Andrew D. Abel