Вопросы к экзамену по дисциплине "Юридическая ответственность должностных лиц органов государственной власти" для магистрантов специальности «Правовое обеспечение публичной власти»

Nosema ceranae is now a widespread honey bee pathogen with high incidence in apiculture. Rapid and reliable detection and quantification methods are a matter of concern for research community, nowadays mainly relying on the use of biomolecular techniques such as PCR, RT-PCR or HRMA. The aim of this technical paper is to provide a new qPCR assay, based on the highly-conserved protein coding gene Hsp70, to detect and quantify the microsporidian Nosema ceranae affecting the western honey bee Apis mellifera. The validation steps to assess efficiency, sensitivity, specificity and robustness of the assay are described also

Beyond pain: can antidepressants improve depressive symptoms and quality of life in patients with neuropathic pain? A systematic review and meta-analysis

Neuropathic pain can be a predictor of severe emotional distress, up to full blown depressive states. In these patients, it is important to move beyond the sole treatment of pain, in order to recognize depressive symptoms, and to ultimately improve the quality of life.We systematically searched for published and unpublished clinical trials assessing the efficacy and tolerability of antidepressants versus placebo on depression, anxiety and quality of life in patients with neuropathic pain, and pooled data in a meta-analysis.A total of 37 studies fulfilled eligibility criteria and 32 provided data for meta-analysis. Antidepressants were more effective than placebo in improving depressive symptoms (standardized mean difference -0.11; 95% confidence interval -0.20 to -0.02), although the magnitude of effect was small, with a number-needed-to-treat of 24. No significant difference emerged between antidepressants and placebo in reducing anxiety. Quality of life appeared improved in patients on antidepressants, as did pain. Acceptability and tolerability were higher in patients on placebo.To our knowledge, this is the first meta-analysis specifically focusing on the effect of antidepressants on psychiatric symptoms and quality of life in patients with neuropathic pain. Our findings suggest that, despite their potential benefit in patients with neuropathic pain, antidepressants should be prescribed with particular care, as they might be less tolerable in such a fragile population. However, our findings warrant further research to explore how a correct use of antidepressants can help patients to cope with the consequences of neuropathic pain on their psychosocial health and quality of life

I demoni di Napoli. Naturale, preternaturale, sovrannaturale a Napoli e nell'Europa di età moderna

La possessione diabolica e l'arte esorcistica, le apparizioni e il culto dei santi, la medicina dotta e i rimedi popolari, il sonnambulism o e lo spiritismo: sono mappe di un percorso all'interno del "meraviglioso" napoletano di età moderna, presentato nelle sue diverse sfaccettature

FANCM c.5791C > T nonsense mutation (rs144567652) induces exon skipping, affects DNA repair activity and is a familial breast cancer risk factor

Numerous genetic factors that influence breast cancer risk are known. However, approximately two-thirds of the overall familial risk remain unexplained. To determine whether some of the missing heritability is due to rare variants conferring high to moderate risk, we tested for an association between the c.5791C>T nonsense mutation (p.Arg1931*; rs144567652) in exon 22 of FANCM gene and breast cancer. An analysis of genotyping data from 8635 familial breast cancer cases and 6625 controls from different countries yielded an association between the c.5791C>T mutation and breast cancer risk [ odds ratio (OR) = 3.93 (95% confidence interval (CI) = 1.28-12.11; P = 0.017)]. Moreover, we performed two meta-analyses of studies from countries with carriers in both cases and controls and of all available data. These analyses showed breast cancer associations with OR = 3.67 (95% CI = 1.04-12.87; P = 0.043) and OR = 3.33 (95% CI = 1.09-13.62; P = 0.032), respectively. Based on information theory-based prediction, we established that the mutation caused an out-of-frame deletion of exon 22, due to the creation of a binding site for the pre-mRNA processing protein hnRNP A1. Furthermore, genetic complementation analyses showed that the mutation influenced the DNA repair activity of the FANCM protein. In summary, we provide evidence for the first time showing that the common p.Arg1931* loss-of-function variant in FANCM is a risk factor for familial breast cancer