Significance of Capacitative Ca 2+ Entry in the Regulation of Phosphatidylserine Expression at the Surface of Stimulated Cells †

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Parallel FISH and Immunohistochemical Studies of ALK Status in 3244 Non-Small-Cell Lung Cancers Reveal Major Discordances

International audienceIntroduction: Anaplastic lymphoma kinase (ALK) rearrangements occur in 1% to 7% of non-small-cell lung cancers (NSCLCs). Crizotinib, an ALK inhibitor, has been demonstrated to provide dramatic clinical benefits in ALK-positive advanced-stage NSCLC. Fluorescent in situ hybridization (FISH) has been established in clinical trials as the standard procedure method for detecting ALK rearrangements. Although the detection of ALK by immunohistochemistry (IHC) has been proposed for the screening of patients, large-scale studies are warranted to validate such a hierarchical approach. Methods: In this article, we report the largest series thus far of parallel FISH and IHC ALK testing in 3244 consecutive NSCLC cases analyzed at two independent French centers. Results: FISH-positive and/or IHC-positive results were demonstrated in 150 of 3244 cases (4.6%). An imbalanced sex ratio was detected, with women exhibiting a 2.2-fold relative risk for an alteration. Strikingly, only 80 of 150 specimens were classified as ALK positive by both techniques. The specimens with discordant FISH/IHC analyses were FISH-positive/IHC-negative (36), FISH-negative/IHC-positive (19), or FISH-noncontributive/IHC-positive (15). Thus, a single FISH or IHC analysis performed alone would have failed to detect approximately one-fourth of the ALK-positive cases with similar findings in our two centers. Conclusions: This study highlights the feasibility of systematic NSCLC testing by both FISH and IHC in routine practice. Many preanalytical factors may account for the apparent discrepancies between both methods, suggesting that hierarchical screening may underscore ALK-positive cases. This significant level of discrepancy supports the need of combined testing to optimize the detection of ALK-inhibitor-eligible patients given that some patients with discordant testing were found to respond to crizotinib

Animal symbolisé, animal exploité : du Paléolithique à la Protohistoire. Actes du 141ème Congrès du CTHS « L’animal et l’homme », Rouen, 11-16 avril 2016

International audienceLes dix-neuf textes que comporte ce volume sont issus du colloque organisé dans le cadre du 141ème Congrès du CTHS « L’animal et l’homme » qui s’est déroulé à Rouen du 11 au 16 avril 2016. Proposé par la section préhistoire et protohistoire du CTHS, ce colloque avait pour objectif d’aborder les relations Homme/Animal sous ses multiples facettes, depuis le Paléolithique jusqu’à l’Âge du Fer. La trentaine de communications proposée lors de ces journées a porté sur cinq grandes thématiques « L’animal ressource », « Entre chien et loup : de la bête féroce à l’animal familier », « Les animaux vus et perçus par les Paléolithiques », « La vie et la mort partagée », « Pré-histoire des échanges de pathogènes »