Impact of Vegetative Treatment Systems on Multiple Measures of Antibiotic Resistance in Agricultural Wastewater

Wastewater is an important vector of antibiotic resistant bacteria and antibiotic resistance genes (ARB/G). While there is broad agreement that ARB/G from agricultural (ag) wastewaters can be transported through the environment and may contribute to untreatable infectious disease in humans and animals, there remain large knowledge gaps surrounding applied details on the types and amounts of ARB/G associated with different agricultural wastewater treatment options and different ag production systems. This study evaluates a vegetative treatment system (VTS) built to treat the wastewater from a beef cattle feedlot. Samples were collected for three years, and plated on multiple media types to enumerate tetracycline and cefotaxime-resistant bacteria. Enterobacteriaceae isolates (n = 822) were characterized for carriage of tetracycline resistance genes, and E. coli isolates (n = 673) were phenotyped to determine multi-drug resistance (MDR) profiles. Tetracycline resistance in feedlot runoff wastewater was 2-to-3 orders of magnitude higher compared to rainfall runoff from the VTS fields, indicating efficacy of the VTA for reducing ARB over time following wastewater application. Clear differences in MDR profiles were observed based on the specific media on which a sample was plated. This result highlights the importance of method, especially in the context of isolate-based surveillance and monitoring of ARB in agricultural wastewaters

Impact of Vegetative Treatment Systems on Multiple Measures of Antibiotic Resistance in Agricultural Wastewater

Wastewater is an important vector of antibiotic resistant bacteria and antibiotic resistance genes (ARB/G). While there is broad agreement that ARB/G from agricultural (ag) wastewaters can be transported through the environment and may contribute to untreatable infectious disease in humans and animals, there remain large knowledge gaps surrounding applied details on the types and amounts of ARB/G associated with different agricultural wastewater treatment options and different ag production systems. This study evaluates a vegetative treatment system (VTS) built to treat the wastewater from a beef cattle feedlot. Samples were collected for three years, and plated on multiple media types to enumerate tetracycline and cefotaxime-resistant bacteria. Enterobacteriaceae isolates (n = 822) were characterized for carriage of tetracycline resistance genes, and E. coli isolates (n = 673) were phenotyped to determine multi-drug resistance (MDR) profiles. Tetracycline resistance in feedlot runoff wastewater was 2-to-3 orders of magnitude higher compared to rainfall runoff from the VTS fields, indicating efficacy of the VTA for reducing ARB over time following wastewater application. Clear differences in MDR profiles were observed based on the specific media on which a sample was plated. This result highlights the importance of method, especially in the context of isolate-based surveillance and monitoring of ARB in agricultural wastewaters

Nuclear Saturation with in-Medium Meson Exchange Interactions

We show that the assumption of dropping meson masses together with conventional many-body effects, implemented in the relativistic Dirac-Brueckner formalism, explains nuclear saturation. We use a microscopic model for correlated $2\pi$ exchange and include the standard many-body effects on the in-medium pion propagation, which initially increase the attractive nucleon-nucleon ($NN$) potential with density. For the vector meson exchanges in both the $\pi\pi$ and $NN$ sector, we assume Brown-Rho scaling which---in concert with `chiral' $\pi\pi$ contact interactions---reduces the attraction at higher densities.Comment: 5 pages REVTeX, 2 eps-figures included, submitted to Phys. Rev. Let

Evaluation of pre-processing on the meta-analysis of DNA methylation data from the Illumina HumanMethylation450 BeadChip platform

Introduction Meta-analysis is a powerful means for leveraging the hundreds of experiments being run worldwide into more statistically powerful analyses. This is also true for the analysis of omic data, including genome-wide DNA methylation. In particular, thousands of DNA methylation profiles generated using the Illumina 450k are stored in the publicly accessible Gene Expression Omnibus (GEO) repository. Often, however, the intensity values produced by the BeadChip (raw data) are not deposited, therefore only pre-processed values -obtained after computational manipulation- are available. Pre-processing is possibly different among studies and may then affect meta-analysis by introducing non-biological sources of variability. Material and methods To systematically investigate the effect of pre-processing on meta-analysis, we analysed four different collections of DNA methylation samples (datasets), each composed of two subsets, for which raw data from controls (i.e. healthy subjects) and cases (i.e. patients) are available. We pre-processed the data from each dataset with nine among the most common pipelines found in literature. Moreover, we evaluated the performance of regRCPqn, a modification of the RCP algorithm that aims to improve data consistency. For each combination of pre-processing (9 7 9), we first evaluated the between-sample variability among control subjects and, then, we identified genomic positions that are differentially methylated between cases and controls (differential analysis). Results and conclusion The pre-processing of DNA methylation data affects both the between-sample variability and the loci identified as differentially methylated, and the effects of pre-processing are strongly dataset-dependent. By contrast, application of our renormalization algorithm regRCPqn: (i) reduces variability and (ii) increases agreement between meta-analysed datasets, both critical components of data harmonization

Two-pion production processes, chiral symmetry and NN interaction in the medium

We study the two-pion propagator in the nuclear medium. This quantity appears in the $\pi-\pi$ T-matrix and we show that it also enters the QCD scalar susceptibility. The medium effects on this propagator are due to the influence of the individual nucleon response to a scalar field through their pion clouds. This response is appreciably increased by the nuclear environment. It produces an important convergence effect between the scalar and pseudoscalar susceptibilities, reflecting the reshaping of the scalar strengh observed in $2\pi$ production experiments. While a large modification of the $\sigma$ propagator follows, due to its coupling to two pion states, we show that the NN potential remains instead unaffected.Comment: 14 pages, 4 figures, submitted to EPJ

Threatened and Invasive Reptiles Are Not Two Sides of the Same Coin

The ‘two sides of the same coin’ hypothesis posits that biological traits that predispose species to extinction and invasion lie on opposite ends of a continuum. Conversely, anthropogenic factors may have similar effects on extinction and invasion risk. We test these two hypotheses using data on more than 1000 reptile species

What does `rho-exchange' in piN scattering mean?

We present an alternative method for calculating amplitudes for correlated pi pi exchange in the ``sigma'' and rho channel in piN scattering. Starting from a fixed mass meson exchange potential, we introduce the width of the exchanged particles by integrating over a mass spectral function. The spectral functions are constructed from the pseudoempirical N\bar{N} -> pi pi data. Using this approach we develop a prescription for resolving ambiguities of the correlated pi pi exchange in the rho channel that occur when different dispersion theoretical formulations of rho exchange are used to construct piN potentials.Comment: 17 pages, 5 figures, uses revtex and epsfi

N N bar,Delta bar N, Delta N bar excitation for the pion propagator in nuclear matter

The particle-hole and Delta -hole excitations are well-known elementary excitation modes for the pion propagator in nuclear matter. But, the excitation also involves antiparticles, namely, nucleon-antinucleon, anti-Delta-nucleon and Delta-antinucleon excitations. These are important for high-energy momentum as well, and have not been studied before, to our knowledge. In this paper, we give both the formulas and the numerical calculations for the real and the imaginary parts of these excitations.Comment: Latex, 3 eps file

Medium Modification of The Pion-Pion Interaction at Finite Density

We discuss medium modifications of the unitarized pion-pion interaction in the nuclear medium. We incorporate both the effects of chiral symmetry restoration and the influence of collective nuclear pionic modes originating from the p-wave coupling of the pion to delta-hole configurations. We show in particular that the dropping of the sigma meson mass significantly enhances the low energy structure created by the in-medium collective pionic modes.Comment: 26 pages, 7 figures included, Latex fil