Characterization of Soil-Water Retention with Coarse Fragments in the Densu Basin of Ghana

The presence of coarse fragments can have profound impact on soil moisture retention characteristics. The study was conducted to assess the effects of coarse fragments on the moisture retention characteristics of 16 soil series, developed over five different parent materials in the Densu basin. Soil profiles were excavated at five locations, to depths within 1.5 m in the field. Undisturbed soil core samples and disturbed samples were taken in triplicates from the major genetic horizons of each soil type within the effective root depth of 1 m. Coarse fragments content of soil more than 2 mm was measured on mass basis by sieving through a 2-mm mesh. Soil moisture retention was determined using the pressure plate apparatus at suctions of pF 1 (1.0 kPa), pF 2 (10.0 kPa), pF 2.5 (33.0 kPa) and pF 3 (100.0 kPa) for the undisturbed and pF 4.2 (1500 kPa) for the disturbed samples.The volumetric moisture content between field capacity (FC) pF 2.5 (33.0 kPa) and permanent wilting pointing (PWP) pF 4.2 (1500.0 kPa) was used to evaluate the available water content (AWC) by volume and then converted to root zone available water capacity (RZAWC) in millimetres (mm) assuming an effective root depth of 1 m within the basin. Results showed that soils formed over granite and its associations have high percentage of coarse fragments while soils developed over phyllites and its associations have high clay percentage. Soil organic matter was high in the topsoil of all profiles, ranging from 0.81 to 4.44% compared with the horizons below, and the bulk density of the topsoils were less than the limiting value of 1.6 Mg m-3. Site-specific moisture retention characteristics of the various soil series have been delineated. It was evident from the analyses that soils containing high clay content gave high RZAWC values compared with soils with high coarse fragments. Most of the topsoils of the profiles gave high RZAWC values compared with sub-layers with high amounts of coarse fragments. Critical water for plants establishment within the basin in the surface layer was quite favourable

Metabolic Profiling of Children Undergoing Surgery for Congenital Heart Disease.

OBJECTIVE: Inflammation and metabolism are closely interlinked. Both undergo significant dysregulation following surgery for congenital heart disease, contributing to organ failure and morbidity. In this study, we combined cytokine and metabolic profiling to examine the effect of postoperative tight glycemic control compared with conventional blood glucose management on metabolic and inflammatory outcomes in children undergoing congenital heart surgery. The aim was to evaluate changes in key metabolites following congenital heart surgery and to examine the potential of metabolic profiling for stratifying patients in terms of expected clinical outcomes. DESIGN: Laboratory and clinical study. SETTING: University Hospital and Laboratory. PATIENTS: Of 28 children undergoing surgery for congenital heart disease, 15 underwent tight glycemic control postoperatively and 13 were treated conventionally. INTERVENTIONS: Metabolic profiling of blood plasma was undertaken using proton nuclear magnetic resonance spectroscopy. A panel of metabolites was measured using a curve-fitting algorithm. Inflammatory cytokines were measured by enzyme-linked immunosorbent assay. The data were assessed with respect to clinical markers of disease severity (Risk Adjusted Congenital heart surgery score-1, Pediatric Logistic Organ Dysfunction, inotrope score, duration of ventilation and pediatric ICU-free days). MEASUREMENTS AND MAIN RESULTS: Changes in metabolic and inflammatory profiles were seen over the time course from surgery to recovery, compared with the preoperative state. Tight glycemic control did not significantly alter the response profile. We identified eight metabolites (3-D-hydroxybutyrate, acetone, acetoacetate, citrate, lactate, creatine, creatinine, and alanine) associated with surgical and disease severity. The strength of proinflammatory response, particularly interleukin-8 and interleukin-6 concentrations, inversely correlated with PICU-free days at 28 days. The interleukin-6/interleukin-10 ratio directly correlated with plasma lactate. CONCLUSIONS: This is the first report on the metabolic response to cardiac surgery in children. Using nuclear magnetic resonance to monitor the patient journey, we identified metabolites whose concentrations and trajectory appeared to be associated with clinical outcome. Metabolic profiling could be useful for patient stratification and directing investigations of clinical interventions.Mr. Correia is supported by the Imperial College Stratified Medicine Graduate Training Programme in Systems Medicine and Spectroscopic Profiling (STRATiGRAD). Dr. Pathan’s institution received grant support from the British Heart Foundation (research grant) and a Higher Education Funding Council for England clinical senior lecturer award. Dr. Ng received grant support from the British Heart Foundation (Researcher salary) and received support for article research from the British Heart Foundation. Dr. Jimenez consulted for Metabometrix is employed by the Imperial College London, and received support for article research from the National Institutes of Health (NIH). Her institution received grant support from the Cardiovascular Biomedical Research Institute. Dr. Macrae is employed by Royal Brompton and Harefield NHS Foundation Trust. Dr. Holmes is employed by the Imperial College London and received support for article research. Her institution received grant support from the Imperial College London (unrelated research grants in the field of metabolic medicine). The remaining authors have disclosed that they do not have any potential conflicts of interest.This is the final published version. It first appeared at http://journals.lww.com/ccmjournal/pages/articleviewer.aspx?year=9000&issue=00000&article=97295&type=abstract

Decision support tools for site-specific fertilizer recommendations and agricultural planning in selected countries in sub-Sahara Africa

Peer Revie

Polyamide-Scorpion Cyclam Lexitropsins Selectively Bind AT-Rich DNA Independently of the Nature of the Coordinated Metal

Cyclam was attached to 1-, 2- and 3-pyrrole lexitropsins for the first time through a synthetically facile copper-catalyzed “click” reaction. The corresponding copper and zinc complexes were synthesized and characterized. The ligand and its complexes bound AT-rich DNA selectively over GC-rich DNA, and the thermodynamic profile of the binding was evaluated by isothermal titration calorimetry. The metal, encapsulated in a scorpion azamacrocyclic complex, did not affect the binding, which was dominated by the organic tail