Efeito de soluções conservadores renais utilizadas no transplante clínico e experimental sobre a condutividade hidráulica de túbulos coletores corticais de coelho isolados e perfundidos in vitro

BV UNIFESP: Teses e dissertaçõe

Estudos da ação da solução preservadora de Collins em túbulos contornados proximais de coelhos isolados e perfundidos in vitro

BV UNIFESP: Teses e dissertaçõe

The Cystic Fibrosis Transmembrane Regulator (CFTR) in the kidney

The cystic fibrosis transmembrane regulator (CFTR) is a Cl- channel. Mutations of this transporter lead to a defect of chloride secretion by epithelial cells causing the Cystic Fibrosis disease (CF). In spite of the high expression of CFTR in the kidney, patients with CF do not show major renal dysfunction, but it is known that both the urinary excretion of drugs and the renal capacity to concentrate and dilute urine is deficient. CFTR mRNA is expressed in all nephron segments and its protein is involved with chloride secretion in the distal tubule, and the principal cells of the cortical (CCD) and medullary (IMCD) collecting ducts. Several studies have demonstrated that CFTR does not only transport Cl- but also secretes ATP and, thus, controls other conductances such as Na+ (ENaC) and K+ (ROMK2) channels, especially in CCD. In the polycystic kidney the secretion of chloride through CFTR contributes to the cyst enlargement. This review is focused on the role of CFTR in the kidney and the implications of extracellular volume regulators, such as hormones, on its function and expression

PROPERTIES OF THE ISOLATED RABBIT PROXIMAL CONVOLUTED TUBULE AND PARS RECTA AFTER LONG-TERM STORAGE IN A MODIFIED COLLINS SOLUTION

ESCOLA PAULISTA MED,COLL MED,DISCIPLINA NEFROL,RUA BOTUCATU,BR-04023 SAO PAULO,SP,BRAZILESCOLA PAULISTA MED,COLL MED,DISCIPLINA NEFROL,RUA BOTUCATU,BR-04023 SAO PAULO,SP,BRAZILWeb of Scienc

Apical Plasma Membrane Mispolarization of NaK-ATPase in Polycystic Kidney Disease Epithelia Is Associated with Aberrant Expression of the β2 Isoform

Autosomal dominant polycystic kidney disease (ADPKD) is a common genetic disease of the kidney, characterized by cystic enlargement of renal tubules, aberrant epithelial proliferation, and ion and fluid secretion into the lumen. Previous studies have shown abnormalities in polarization of membrane proteins, including mislocalization of the NaK-ATPase to the apical plasma membranes of cystic epithelia. Apically located NaK-ATPase has previously been shown to be fully functional in vivo and in membrane-grown ADPKD epithelial cells in vitro, where basal-to-apical 22Na transport was inhibited by application of ouabain to the apical membrane compartment. Studies were conducted with polymerase chain reaction-generated specific riboprobes and polyclonal peptide antibodies against human sequences of α1, α3, β1, and β2 subunits of NaK-ATPase. High levels of expression of α1 and β1 messenger RNA were detected in ADPKD and age-matched normal adult kidneys in vivo, whereas β2 messenger RNA was detected only in ADPKD kidneys. Western blot analysis and immunocytochemical studies showed that, in normal adult kidneys, peptide subunit-specific antibodies against α1 and β1 localized to the basolateral membranes of normal renal tubules, predominantly thick ascending limbs of Henle’s loop. In ADPKD kidneys, α1 and β2 subunits were localized to the apical epithelial cell membranes, whereas β1 was distributed throughout the cytoplasm and predominantly in the endoplasmic reticulum, but was not seen associated with cystic epithelial cell membranes or in cell membrane fractions. Polarizing, renal-derived epithelial Madin Darby canine kidney cells, stably expressing normal or N-terminally truncated chicken β1 subunits, showed selective accumulation in the basolateral Madin Darby canine kidney cell surface, whereas c-myc epitope-tagged chicken β2 or human β2 subunits accumulated selectively in the apical cell surface. Similarly, human ADPKD epithelial cell lines, which endogenously expressed α1 and β2 NaK-ATPase subunits, showed colocalization at the apical cell surface and coassociation by immunoprecipitation analysis. These results are consistent with a model in which the additional transcription and translation of the β2 subunit of NaK-ATPase may result in the apical mislocalization of NaK-ATPase in ADPKD cystic epithelia

RELEASE OF LACTATE-DEHYDROGENASE ACTIVITY BY RABBIT RENAL-CORTEX SLICES DURING STORAGE IN SOLUTIONS USED FOR KIDNEY-TRANSPLANTATION

ESCOLA PAULISTA MED,DEPT CLIN MED,DISCIPLINA NEFROL,RUA BOTUCATU 740,BR-04023 SAO PAULO,SP,BRAZILESCOLA PAULISTA MED,DEPT CLIN MED,DISCIPLINA NEFROL,RUA BOTUCATU 740,BR-04023 SAO PAULO,SP,BRAZILWeb of Scienc

Sanierung von Bergbauwaessern aus Altablagerungen des Schieferbergbaus Anlagen 1-6. Zusammenfassender Ergebnisbericht

With CD-ROMSIGLEAvailable from TIB Hannover: Available from TIB Hannover: F99B900(1): F99B900(2) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDeutsche Bundesstiftung Umwelt, Osnabrueck (Germany)DEGerman