Single-nanoparticle phase transitions visualized by four-dimensional electron microscopy

The advancement of techniques that can probe the behaviour of individual nanoscopic objects is of paramount importance in various disciplines, including photonics and electronics. As it provides images with a spatiotemporal resolution, four-dimensional electron microscopy, in principle, should enable the visualization of single-nanoparticle structural dynamics in real and reciprocal space. Here, we demonstrate the selectivity and sensitivity of the technique by visualizing the spin crossover dynamics of single, isolated metal–organic framework nanocrystals. By introducing a small aperture in the microscope, it was possible to follow the phase transition and the associated structural dynamics within a single particle. Its behaviour was observed to be distinct from that imaged by averaging over ensembles of heterogeneous nanoparticles. The approach reported here has potential applications in other nanosystems and those that undergo (bio)chemical transformations

Azacytidine and Decitabine Induce Gene-Specific and Non-Random DNA Demethylation in Human Cancer Cell Lines

The DNA methyltransferase inhibitors azacytidine and decitabine represent archetypal drugs for epigenetic cancer therapy. To characterize the demethylating activity of azacytidine and decitabine we treated colon cancer and leukemic cells with both drugs and used array-based DNA methylation analysis of more than 14,000 gene promoters. Additionally, drug-induced demethylation was compared to methylation patterns of isogenic colon cancer cells lacking both DNA methyltransferase 1 (DNMT1) and DNMT3B. We show that drug-induced demethylation patterns are highly specific, non-random and reproducible, indicating targeted remethylation of specific loci after replication. Correspondingly, we found that CG dinucleotides within CG islands became preferentially remethylated, indicating a role for DNA sequence context. We also identified a subset of genes that were never demethylated by drug treatment, either in colon cancer or in leukemic cell lines. These demethylation-resistant genes were enriched for Polycomb Repressive Complex 2 components in embryonic stem cells and for transcription factor binding motifs not present in demethylated genes. Our results provide detailed insights into the DNA methylation patterns induced by azacytidine and decitabine and suggest the involvement of complex regulatory mechanisms in drug-induced DNA demethylation

Mesoscale Atmospheric Transport of Ragweed Pollen Allergens from Infected to Uninfected Areas

Allergenic ragweed (Ambrosia spp.) pollen grains, after being released from anthers, can be dispersed by air masses far from their source. However, the action of air temperature,humidity and solar radiation on pollen grains in the atmosphere could impact on the ability of long distance transported (LDT) pollen to maintain allergenic potency. Here, we report that the major allergen of Ambrosia artemisiifolia pollen (Amb a 1) collected in ambient air during episodes of LDT still have immunoreactive properties. The amount of Amb a 1 found in LDT ragweed pollen grains was not constant and varied between episodes. In addition to allergens in pollen sized particles, we detected reactive Amb a 1 in subpollen sized respirable particles. These findings suggest that ragweed pollen grains have the potential to cause allergic reactions, not only in the heavily infested areas but, due to LDT episodes, also in the regions unaffected by ragweed populations

Study of the diversity of culturable actinomycetes in the North Pacific and Caribbean coasts of Costa Rica

In this study, 137 actinomycetes were isolated from subtidal marine sediments in the North Pacific and Caribbean coasts of Costa Rica. Bioinformatics analysis of the 16S rRNA gene sequences assigned the isolates to 15 families and 21 genera. Streptomyces was the dominant genus while the remaining 20 genera were poorly represented. Nearly 70% of the phylotypes presented a coastal-restricted distribution whereas the other 30% were common inhabitants of both shores. The coastal tropical waters of Costa Rica showed a high diversity of actinomycetes, both in terms of the number of species and phylogenetic composition, although significant differences were observed between and within shores. The observed pattern of species distribution might be the result of several factors including the characteristics of the ecosystems, presence of endemic species and the influence of terrestrial runoff.University of Aberdeen/[]//EscociaNational Institutes of Health/[U01 TW007404-01 ICBG]/NIH/Estados UnidosUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de BiologíaUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Químic

The nature of sub-millimetre galaxies II: an ALMA comparison of SMG dust heating mechanisms

We compare the contribution of active galactic nuclei (AGNs) and star formation towards dust heating in sub-mm galaxies (SMGs). We have used ALMA at 0.1-arcsec resolution to image a complete flux-limited sample of seven sub-mm sources previously shown to have spectral energy distributions that were as well-fitted by obscured AGN as star-forming galaxy templates. Indeed, two sub-mm sources were known to be quasars from their absorbed X-ray emission. We find the sub-mm sizes of all SMGs to be small (≈1−2 kpc) and generally ∼3 times smaller than any host detected in the near-infrared (NIR). In all cases, the five SMGs are comparable in sub-mm size to the two known quasars and four z ≈ 6 quasars, also observed with ALMA. We detect no evidence of diffuse spiral arms in this complete sample. We then convert the far-infrared (FIR) luminosities to star formation rate (SFR) surface densities and find that the SMGs occupy the same range as the known quasars in our sample. We conclude that in terms of sub-mm size, extent relative to host and SFR density as well as luminosity and mid-IR (MIR) colour, there is little distinction between the SMGs and sub-mm bright quasars. Finally, we present preliminary evidence that SMGs with higher MIR luminosities and sub-mm loud quasars tend to have dust components that range to hotter temperatures than their less luminous SMG counterparts. In light of these results, we continue to suggest that luminous SMGs may host dust-absorbed quasars that may simultaneously dominate the FIR and hard X-ray backgrounds

KASHz: No evidence for ionised outflows instantaneously suppressing star formation in moderate luminosity AGN at z∼1.4–2.6

As part of our KMOS AGN Survey at High-redshift (KASHz), we present spatially-resolved VLT/KMOS and VLT/SINFONI spectroscopic data and ALMA 870μm continuum imaging of eight z=1.4–2.6 moderate AGN (⁠L 2−10kev L2−10kev = 1042 − 1045 ergs s−1). We map [O iii], Hα and rest-frame FIR emission to search for any spatial anti-correlation between ionised outflows (traced by the [O iii] line) and star formation (SF; traced by Hα and FIR), that has previously been claimed for some high-z AGN and used as evidence for negative and/or positive AGN feedback. Firstly, we conclude that Hα is unreliable to map SF inside our AGN host galaxies based on: (i) SF rates inferred from attenuation-corrected Hα can lie below those inferred from FIR; (ii) the FIR continuum is more compact than the Hα emission by a factor of ∼2 on average; (iii) in half of our sample, we observe significant spatial offsets between the FIR and Hα emission, with an average offset of 1.4 ± 0.6 kpc. Secondly, for the five targets with outflows we find no evidence for a spatial anti-correlation between outflows and SF using either Hα or FIR as a tracer. This holds for our re-analysis of a famous z=1.6 X-ray AGN (‘XID 2028’) where positive and negative feedback has been previously claimed. Based on our results, any impact on SF by ionised outflows must be subtle, either occurring on scales below our resolution, or on long timescales