Patterns of Sponge Abundance Across a Gradient of Habitat Quality in the Wakatobi Marine National Park, Indonesia

Sponges are important components of reef communities worldwide, fulfilling a number of important functional roles. Habitat degradation caused by the loss of hard corals has the potential to cause increases in sponge abundance and percentage cover as they gain access to resources such as space and food. In this study we compared sponge densities and percentage cover at sites with varying hard coral cover in the Wakatobi Marine National Park, Indonesia (WMNP). We found significant differences in sponge densities at the study sites but no significant difference in sponge densities on different surface angles. Unexpectedly, we also found a weak positive correlation between coral cover and sponge density. This indicates that spatial competition is unlikely to be the most important factor determining sponge abundance in the WMNP. In contrast to sponge density data, we found that sponge percentage cover and hard coral cover were weakly negatively correlated, but found no significant difference in sponge percentage cover between the study sites. Finally, multivariate analysis of the benthic communities at the study sites indicated that while sites with higher coral cover were characterised by coral (proportionally), lower coral cover sites were characterised by algae and sponges. This suggests that although there was no significant difference in sponge percentage cover between the study sites conditions that led to the loss of hard coral at lower quality sites mean that these sites are characterised by sponges and algae rather than by any other groups of benthic organisms

Lean supply chain management and Industry 4.0: a systematic literature review

Purpose Even though the integration of Lean Supply Chain Management (LSCM) and Industry 4.0 (I4.0) technologies is relatively recent, it has been receiving a lot of attention. Partly because it is a recent field of practise and research and partly because the number of works developed in this field has grown rapidly, it is important to frequently update the perspectives on this field of investigation. Thus, this study aims to review the integration between LSCM and I4.0 analysing relationship at operative, tactical and strategic levels. Design/methodology/approach Systematic literature review was conducted to identify and explain the integration of LSCM and I4.0 from scientific sources that were published before March 2021. Findings The analysis of the literature revealed the level of integration of LSCM and I4.0 is present at different managerial levels. Moreover, when the integration is detailed at different managerial levels, it appears that LSCM paves the way for the adoption of I4.0 at a strategic level, while I4.0 technologies promise to enhance LSCM practices at the operational level. Research limitations/implications The main contribution of this study is the framework which shows that LSCM paves the way for the adoption of I4.0 at a strategic level, while I4.0 technologies promise to enhance LSCM practices at the operational level. Originality/value This study develops a new perspective of the articles published under the thematic of LSCM and I4.0. Additionally, it proposes a framework of analysis that can be used by future researchers. Finally, it shows the most recent implementations of LSCM and I4.0, exposing the current trends, improvements and also the main gaps

Genomics and successful aging: grounds for renewed optimism?

This is a pre-copyedited, author-produced PDF of an article accepted for publication in the Journals of Gerentology following peer review. The version of record is available online at: doi: 10.1093/gerona/gls091.Successful aging depends in part on delaying age-related disease onsets until later in life. Conditions including coronary artery disease, Alzheimer's disease, prostate cancer, and type 2 diabetes are moderately heritable. Genome-wide association studies have identified many risk associated single-nucleotide polymorphisms for these conditions, but much heritability remains unaccounted for. Nevertheless, a great deal is being learned.Dunhill Medical TrustU.S. National Institutes of Healt

Let me Google that for you:a time series analysis of seasonality in internet search trends for terms related to foot and ankle pain

BACKGROUND: The analysis of internet search traffic may present the opportunity to gain insights into general trends and patterns in information seeking behaviour related to medical conditions at a population level. For prevalent and widespread problems such as foot and ankle pain, this information has the potential to improve our understanding of seasonality and trends within these conditions and their treatments, and may act as a useful proxy for their true incidence/prevalence characteristics. This study aimed to explore seasonal effects, general trends and relative popularity of internet search terms related to foot and ankle pain over the past decade. METHODS: We used the Google Trends tool to obtain relative search engine traffic for terms relating to foot and ankle pain and common treatments from Google search and affiliated pages for major northern and southern hemisphere English speaking nations. Analysis of overall trends and seasonality including summer/winter differences was carried out on these terms. RESULTS: Searches relating to general foot pain were on average 3.4 times more common than those relating to ankle pain, and twice as common as searches relating to heel pain. Distinct seasonal effects were seen in the northern hemisphere, with large increases in search volumes in the summer months compared to winter for foot (p = 0.004, 95 % CI [22.2–32.1]), ankle (p = 0.0078, 95 % CI [20.9–35.5]), and heel pain (p = 0.004, 95 % CI [29.1–45.6]). These seasonal effects were reflected by data from Australia, with the exception of ankle pain. Annual seasonal effects for treatment options were limited to terms related to foot surgery and ankle orthoses (p = 0.031, 95 % CI [3.5–20.9]; p = 0.004, 95 % CI [7.6–25.2] respectively), again increasing in the summer months. CONCLUSIONS: A number of general trends and annual seasonal effects were found in time series internet search data for terms relating to foot and ankle pain. This data may provide insights into these conditions at population levels. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13047-015-0074-9) contains supplementary material, which is available to authorized users

A novel role for Lyl1 in primitive erythropoiesis

© 2018. Published by The Company of Biologists Ltd. Stem cell leukemia (Scl or Tal1) and lymphoblastic leukemia 1 (Lyl1) encode highly related members of the basic helix-loop-helix family of transcription factors that are co-expressed in the erythroid lineage. Previous studies have suggested that Scl is essential for primitive erythropoiesis. However, analysis of single-cell RNA-seq data of early embryos showed that primitive erythroid cells express both Scl and Lyl1. Therefore, to determine whether Lyl1 can function in primitive erythropoiesis, we crossed conditional Scl knockout mice with mice expressing a Cre recombinase under the control of the Epo receptor, active in erythroid progenitors. Embryos with 20% expression of Scl from E9.5 survived to adulthood. However, mice with reduced expression of Scl and absence of Lyl1 (double knockout; DKO) died at E10.5 because of progressive loss of erythropoiesis. Gene expression profiling of DKO yolk sacs revealed loss of Gata1 and many of the known target genes of the SCL-GATA1 complex. ChIP-seq analyses in a human erythroleukemia cell line showed that LYL1 exclusively bound a small subset of SCL targets including GATA1. Together, these data show for the first time that Lyl1 can maintain primitive erythropoiesis

A review of standard pharmacological therapy for adult asthma - Steps 1 to 5

The aim of pharmacological therapy for asthma is to improve symptoms and lung function and minimise the risk of asthma attacks. The intensity of treatment is based on the level of asthma control and the potential risk of future deterioration. In the British asthma guidelines, treatments are divided into Step One to Five, with each Step signifying a need for an increase in therapy in response to symptoms or to prevent exacerbations. Treatments comprise of inhaled or systemic medications. Inhaled therapy includes short-acting and long-acting medication to improve symptoms and inhaled corticosteroids which reduce airway inflammation. Systemic treatments include medications which act on specific biological pathways, such as the leukotriene or IgE pathways, or systemic corticosteroids. In choosing a particular therapy, treatment benefits are balanced by the potential risks of medication-related adverse effects. This review will provide a practical guide to the key pharmacological therapies for adult asthma at Steps One to Five based on British guidelines and consider future options for new treatments

X-ray radiography of cavitation in a beryllium alloy nozzle

[EN] Making quantitative measurements of the vapor distribution in a cavitating nozzle is difficult, owing to the strong scattering of visible light at gas-liquid boundaries and wall boundaries, and the small lengths and time scales involved. The transparent models required for optical experiments are also limited in terms of maximum pressure and operating life. Over the past few years, x-ray radiography experiments at Argonne's Advanced Photon Source have demonstrated the ability to perform quantitative measurements of the line of sight projected vapor fraction in submerged, cavitating plastic nozzles. In this paper, we present the results of new radiography experiments performed on a submerged beryllium nozzle which is 520m in diameter, with a length/diameter ratio of 6. Beryllium is a light, hard metal that is very transparent to x-rays due to its low atomic number. We present quantitative measurements of cavitation vapor distribution conducted over a range of non-dimensional cavitation and Reynolds numbers, up to values typical of gasoline and diesel fuel injectors. A novel aspect of this work is the ability to quantitatively measure the area contraction along the nozzle with high spatial resolution. Analysis of the vapor distribution, area contraction and discharge coefficients are made between the beryllium nozzle and plastic nozzles of the same nominal geometry. When gas is dissolved in the fuel, the vapor distribution can be quite different from that found in plastic nozzles of the same dimensions, although the discharge coefficients are unaffected. In the beryllium nozzle, there were substantially fewer machining defects to act as nucleation sites for the precipitation of bubbles from dissolved gases in the fuel, and as such the effect on the vapor distribution was greatly reduced.Raul Payri was funded by a Fulbright visiting scholar grant in collaboration with the Ministry of Education, Culture and Sports of Spain (reference PRX14/00331) while performing this work. Juan P Viera was funded by the Spanish MINECO (grant EEBB-I-15-0976 under project TRA2012-36932).Duke, DJ.; Matusik, KE.; Kastengren, AL.; Swantek, AB.; Sovis, N.; Payri, R.; Viera-Sotillo, JP.... (2017). X-ray radiography of cavitation in a beryllium alloy nozzle. International Journal of Engine Research. 18(1-2):39-50. https://doi.org/10.1177/1468087416685965S3950181-

The highly competitive ascidian Didemnum sp. threatens coral reef communities in the Wakatobi Marine National Park, Southeast Sulawesi, Indonesia

Coral reefs in the Wakatobi Marine National Park (WMNP), Indonesia, are protected but have been degrading in several areas due to local anthropogenic stressors. In affected areas, benthic surveys revealed the occurrence of a dominant ascidian species of the genus Didemnum, which may negatively impact the benthic community composition and structure. We quantified the abundance, substrate preference, and growth rate of Didemnum sp. in non-degraded and degraded reefs to assess its impact on the benthic community. While Didemnum sp. occurred in similar high abundances in both, non-degraded (0.66%) and degraded (0.75 %) reef sites, this species showed a substantially (>10-fold) increased growth rate in degrading reefs (2.7 ± 0.98% day−1 increase in colony size, compared to 0.17 ± 0.39 % day −1 in non-degraded reefs). Furthermore, Didemnum sp. colonized many different substrates and showed the ability to overgrow live corals quickly. These observations indicate that Didemnum sp. can be a severe threat to a reef community by outcompeting live corals and call for further studies on the interaction between environmental pollution and Didemnum growth patterns in coral reefs

Enhanced insulin sensitivity associated with provision of mono and polyunsaturated fatty acids in skeletal muscle cells involves counter modulation of PP2A

International audienceAims/Hypothesis: Reduced skeletal muscle insulin sensitivity is a feature associated with sustained exposure to excess saturated fatty acids (SFA), whereas mono and polyunsaturated fatty acids (MUFA and PUFA) not only improve insulin sensitivity but blunt SFA-induced insulin resistance. The mechanisms by which MUFAs and PUFAs institute these favourable changes remain unclear, but may involve stimulating insulin signalling by counter-modulation/repression of protein phosphatase 2A (PP2A). This study investigated the effects of oleic acid (OA; a MUFA), linoleic acid (LOA; a PUFA) and palmitate (PA; a SFA) in cultured myotubes and determined whether changes in insulin signalling can be attributed to PP2A regulation. Principal Findings: We treated cultured skeletal myotubes with unsaturated and saturated fatty acids and evaluated insulin signalling, phosphorylation and methylation status of the catalytic subunit of PP2A. Unlike PA, sustained incubation of rat or human myotubes with OA or LOA significantly enhanced Akt-and ERK1/2-directed insulin signalling. This was not due to heightened upstream IRS1 or PI3K signalling nor to changes in expression of proteins involved in proximal insulin signalling, but was associated with reduced dephosphorylation/inactivation of Akt and ERK1/2. Consistent with this, PA reduced PP2Ac demethylation and tyrosine 307 phosphorylation-events associated with PP2A activation. In contrast, OA and LOA strongly opposed these PA-induced changes in PP2Ac thus exerting a repressive effect on PP2A.Conclusions/Interpretation: Beneficial gains in insulin sensitivity and the ability of unsaturated fatty acids to oppose palmitate-induced insulin resistance in muscle cells may partly be accounted for by counter-modulation of PP2A

Identification of a BRCA1-mRNA Splicing Complex Required for Efficient DNA Repair and Maintenance of Genomic Stability

Mutations within BRCA1 predispose carriers to a high risk of breast and ovarian cancers. BRCA1 functions to maintain genomic stability through the assembly of multiple protein complexes involved in DNA repair, cell-cycle arrest, and transcriptional regulation. Here, we report the identification of a DNA damage-induced BRCA1 protein complex containing BCLAF1 and other key components of the mRNA-splicing machinery. In response to DNA damage, this complex regulates pre-mRNA splicing of a number of genes involved in DNA damage signaling and repair, thereby promoting the stability of these transcripts/proteins. Further, we show that abrogation of this complex results in sensitivity to DNA damage, defective DNA repair, and genomic instability. Interestingly, mutations in a number of proteins found within this complex have been identified in numerous cancer types. These data suggest that regulation of splicing by the BRCA1-mRNA splicing complex plays an important role in the cellular response to DNA damage