A New Chamber for Studying the Behavior of Drosophila

Methods available for quickly and objectively quantifying the behavioral phenotypes of the fruit fly, Drosophila melanogaster, lag behind in sophistication the tools developed for manipulating their genotypes. We have developed a simple, easy-to-replicate, general-purpose experimental chamber for studying the ground-based behaviors of fruit flies. The major innovative feature of our design is that it restricts flies to a shallow volume of space, forcing all behavioral interactions to take place within a monolayer of individuals. The design lessens the frequency that flies occlude or obscure each other, limits the variability in their appearance, and promotes a greater number of flies to move throughout the center of the chamber, thereby increasing the frequency of their interactions. The new chamber design improves the quality of data collected by digital video and was conceived and designed to complement automated machine vision methodologies for studying behavior. Novel and improved methodologies for better quantifying the complex behavioral phenotypes of Drosophila will facilitate studies related to human disease and fundamental questions of behavioral neuroscience

Ingestive behaviour and physiology of the medicinal leech

Ingestion lasts 25 min in Hirudo medicinalis and is characterized by pharyngeal peristalsis which fills the crop. This peristalsis has an initial rate of 2.4 Hz which decays smoothly to 1.2 Hz at termination of ingestion. During ingestion, the leech body wall undergoes peristalsis which appears to aid in filling the crop diverticula. Body peristalsis begins at a rate of 10 min^(-1) and decreases linearly to 2 min^(-1) at termination. The body also undergoes dorsoventral flexions when blood flow is occluded. Blood meal size increases slightly with leech size: 8.4 g for 1-g leeches and 9.7 g for 2-g leeches. However, relative meal size decreases markedly with increasing animal size; from 8.15 times body mass for 1-g to 4.80 times for 2-g leeches. When intact leeches were exposed to micromolar concentrations of serotonin, there was an increase in the rate of pharyngeal peristalsis and the size of the blood meals. Leeches excrete the plasma from their ingested blood meals. Excretion is activated during ingestion, which increases feeding efficiency by increasing the proportion of blood cells in the ingestate. Excretion continues for 4–6 days following ingestion, removing all the remaining plasma from the ingestate. Leech ingestion comprises stereotyped muscular movements, secretion of saliva and excretion of plasma. A strikingly similar feeding physiology is seen in the blood-sucking insect Rhodnius, and we suggest that efficient sanguivory may require the convergent evolution of similar ingestive mechanisms

Public Involvement in research within care homes: Benefits and challenges in the APPROACH Study

Public involvement in research (PIR) can improve research design and recruitment. Less is known about how PIR enhances the experience of participation and enriches the data collection process. In a study to evaluate how UK care homes and primary health care services achieve integrated working to promote older people’s health, PIR was integrated throughout the research processes. Objectives This paper aims to present one way in which PIR has been integrated into the design and delivery of a multi-site research study based in care homes. Design A prospective case study design, with an embedded qualitative evaluation of PIR activity. Setting and Participants Data collection was undertaken in six care homes in three sites in England. Six PIR members participated: all had prior personal or work experience in care homes. Data Collection Qualitative data collection involved discussion groups, and site-specific meetings to review experiences of participation, benefits and challenges, and completion of structured fieldwork notes after each care home visit. Results PIR members supported: recruitment, resident and staff interviews and participated in data interpretation. Benefits of PIR work were resident engagement that minimised distress and made best use of limited research resources. Challenges concerned communication and scheduling. Researcher support for PIR involvement was resource intensive. Discussion and Conclusions Clearly defined roles with identified training and support facilitated involvement in different aspectsPublic Involvement in Research members of the research team: Gail Capstick, Marion Cowie, Derek Hope, Rita Hewitt, Alex Mendoza, John Willmott. Also the involvement of Steven Iliffe and Heather Gag

Using the local gyrokinetic code, GS2, to investigate global ITG modes in tokamaks. (I) s-α{\alpha} model with profile and flow shear effects

This paper combines results from a local gyrokinetic code with analytical theory to reconstruct the global eigenmode structure of the linearly unstable ion-temperature-gradient (ITG) mode with adiabatic electrons. The simulations presented here employ the s-${\alpha}$ tokamak equilibrium model. Local gyrokinetic calculations, using GS2 have been performed over a range of radial surfaces, x, and for ballooning phase angle, p, in the range -${\pi} {\leq} p {\leq\pi}$, to map out the complex local mode frequency, ${\Omega_0(x, p) = \omega_0(x, p) + i\gamma_0(x, p)}$. Assuming a quadratic radial profile for the drive, namely ${\eta_i = L_n/L_T}$, (holding constant all other equilibrium profiles such as safety factor, magnetic shear etc.), ${\Omega_0(x, p)}$ has a stationary point. The reconstructed global mode then sits on the outboard mid plane of the tokamak plasma, and is known as a conventional or isolated mode, with global growth rate, ${\gamma}$ ~ Max[${\gamma_0(x, p)}$], where ${\gamma_0(x, p)}$ is the local growth rate. Taking the radial variation in other equilibrium profiles (e.g safety factor q(x)) into account, removes the stationary point in ${\Omega_0(x, p)}$ and results in a mode that peaks slightly away from the outboard mid-plane with a reduced global growth rate. Finally, the influence of flow shear has also been investigated through a Doppler shift, ${\omega_0 \rightarrow \omega_0 + n\Omega^{\prime}x}$, where n is the toroidal mode number and ${\Omega^{\prime}}$ incorporates the effect of flow shear. The equilibrium profile variation introduces an asymmetry to the growth rate spectrum with respect to the sign of ${\Omega^{\prime}}$, consistent with recent global gyrokinetic calculations.Comment: 10 pages, 8 figures and 1 tabl

Structure of Micro-instabilities in Tokamak Plasmas: Stiff Transport or Plasma Eruptions?

Solutions to a model 2D eigenmode equation describing micro-instabilities in tokamak plasmas are presented that demonstrate a sensitivity of the mode structure and stability to plasma profiles. In narrow regions of parameter space, with special plasma profiles, a maximally unstable mode is found that balloons on the outboard side of the tokamak. This corresponds to the conventional picture of a ballooning mode. However, for most profiles this mode cannot exist and instead a more stable mode is found that balloons closer to the top or bottom of the plasma. Good quantitative agreement with a 1D ballooning analysis is found provided the constraints associated with higher order profile effects, often neglected, are taken into account. A sudden transition from this general mode to the more unstable ballooning mode can occur for a critical flow shear, providing a candidate model for why some experiments observe small plasma eruptions (Edge Localised Modes, or ELMs) in place of large Type I ELMs.Comment: 11 pages, 3 figure

Aeronautic Power Plant Investigations

Report presents the design of radiators, spark plugs and test equipment used to test the performance of aeronautic engines at high altitudes

Sensitivity and foreground modelling for large-scale CMB B-mode polarization satellite missions

The measurement of the large-scale B-mode polarization in the cosmic microwave background (CMB) is a fundamental goal of future CMB experiments. However, because of unprecedented sensitivity, future CMB experiments will be much more sensitive to any imperfect modelling of the Galactic foreground polarization in the reconstruction of the primordial B-mode signal. We compare the sensitivity to B-modes of different concepts of CMB satellite missions (LiteBIRD, COrE, COrE+, PRISM, EPIC, PIXIE) in the presence of Galactic foregrounds. In particular, we quantify the impact on the tensor-to-scalar parameter of incorrect foreground modelling in the component separation process. Using Bayesian fitting and Gibbs sampling, we perform the separation of the CMB and Galactic foreground B-modes. The recovered CMB B-mode power spectrum is used to compute the likelihood distribution of the tensor-to-scalar ratio. We focus the analysis to the very large angular scales that can be probed only by CMB space missions, i.e. the Reionization bump, where primordial B-modes dominate over spurious B-modes induced by gravitational lensing. We find that fitting a single modified blackbody component for thermal dust where the "real" sky consists of two dust components strongly bias the estimation of the tensor-to-scalar ratio by more than 5{\sigma} for the most sensitive experiments. Neglecting in the parametric model the curvature of the synchrotron spectral index may bias the estimated tensor-to-scalar ratio by more than 1{\sigma}. For sensitive CMB experiments, omitting in the foreground modelling a 1% polarized spinning dust component may induce a non-negligible bias in the estimated tensor-to-scalar ratio.Comment: 20 pages, 8 figures, 6 tables. Updated to match version accepted by MNRA

Rectenna system design

The function of the rectenna in the solar power satellite system is described and the basic design choices based on the desired microwave field concentration and ground clearance requirements are given. One important area of concern, from the EMI point of view, harmonic reradiation and scattering from the rectenna is also designed. An optimization of a rectenna system design to minimize costs was performed. The rectenna cost breakdown for a 56 w installation is given as an example

The joint large-scale foreground-CMB posteriors of the 3-year WMAP data

Using a Gibbs sampling algorithm for joint CMB estimation and component separation, we compute the large-scale CMB and foreground posteriors of the 3-yr WMAP temperature data. Our parametric data model includes the cosmological CMB signal and instrumental noise, a single power law foreground component with free amplitude and spectral index for each pixel, a thermal dust template with a single free overall amplitude, and free monopoles and dipoles at each frequency. This simple model yields a surprisingly good fit to the data over the full frequency range from 23 to 94 GHz. We obtain a new estimate of the CMB sky signal and power spectrum, and a new foreground model, including a measurement of the effective spectral index over the high-latitude sky. A particularly significant result is the detection of a common spurious offset in all frequency bands of ~ -13muK, as well as a dipole in the V-band data. Correcting for these is essential when determining the effective spectral index of the foregrounds. We find that our new foreground model is in good agreement with template-based model presented by the WMAP team, but not with their MEM reconstruction. We believe the latter may be at least partially compromised by the residual offsets and dipoles in the data. Fortunately, the CMB power spectrum is not significantly affected by these issues, as our new spectrum is in excellent agreement with that published by the WMAP team. The corresponding cosmological parameters are also virtually unchanged.Comment: 5 pages, 4 figures, submitted to ApJL. Background data are available at http://www.astro.uio.no/~hke under the Research ta