Physiological and biochemical changes occur in onion seeds during ageing

In this study, physiological and biochemical changes at different viability levels were investigated in onion seeds, which lose their viability in a short period of time. For this purpose 95, 80, 60, 40 and 20% viability levels were obtained by controlled deterioration treatments in seeds of onion cvs. Akgun-12, Valencia and TEG-502. Subsequently, mean germination time, electrical conductivity, total lipid, total protein, soluble protein, malondialdehyde (MDA) and catalase activity measurements were conducted at these viability levels. Regarding the seeds of cv. Contes, same measurements were conducted at 80, 60, 40 and 20% viability levels since the initial seed viability was 80%. Depending on seed ageing, as mean germination time, electrical conductivity and MDA content increased, total lipid, total protein, soluble protein contents and catalase activity decreased. Moreover, the increase in mean germination time, MDA content and electrical conductivity and also decrease in catalase activity were more evident when seed viability of each onion cultivar decreased 60% or below this level, which was determined as a threshold.Bu çalışmada canlılığını kısa sürede kaybeden soğan tohumlarında, farklı canlılık seviyelerinde ortaya çıkan bazı fizyolojik ve biyokimyasal değişimler incelenmiştir. Bu amaçla Akgün-12, Valencia ve TEG-502 çeşidi soğan tohumlarında %95, 80, 60, 40 ve 20 canlılık seviyeleri kontrollü bozulma uygulamaları ile elde edilmiştir. Daha sonra bu canlılık seviyelerinde ortalama çimlenme süresi, elektriksel iletkenlik, toplam yağ, toplam protein, çözülebilir protein, malondialdehit (MDA) ve katalaz aktivitesi ölçümleri yapılmıştır. Contes çeşidinde başlangıç canlılığı %80 olması nedeniyle, aynı ölçümler %80, 60, 40 ve 20 canlılık seviyelerinde yapılmıştır. Yaşlanmayla birlikte, ortalama çimlenme süresi, elektriksel iletkenlik ve MDA içeriği artarken, toplam yağ, toplam protein, çözülebilir protein içeriği ve katalaz aktivitesinin azaldığı tespit edilmiştir. Ayrıca, ortalama çimlenme süresi, MDA içeriği ve elektriksel iletkenliğin artması ve katalaz aktivitesinin azalması, tohum canlılığının bir eşik değer olarak belirlenen %60 seviyesine ya da bunun altına düştüğünde daha belirgin olmuştur

QT and JT dispersion in children with familial mediterranean fever

Objectives: This study aims to determine QT dispersion and JT dispersion, and their relationship with conventional echocardiography values in a group of children with familial Mediterranean fever (FMF). Patients and methods: The study included 48 FMF patients (26 males, 22 females, mean age 11.10±3.42 years; range 5 to 18 years) as the FMF patients and 31 healthy children (17 males, 14 females, mean age 9.61±2.83 years; range 5 to 17 years) as the healthy controls. Electrocardiography and conventional echocardiography were performed on the FMF patients and healthy controls. Both groups were evaluated with a standard 12-lead electrocardiography. QT, JT and RR distances were measured in both groups. The corrected QT (QTc) and corrected JT (JTc) were calculated. QTcd and corrected JT dispersion (JTcd) were detected. Results: There was no statistically significant difference between the FMF patients and healthy controls in terms of RR, QT, QTd, QTcd, JT, JTc, JTd, and JTcd measurements and echocardiography parameters. QTc value was higher in the FMF patients than the healthy controls. Conclusion: QTc value indicates increased ventricular sensitivity and is an important marker of cardiovascular mortality. It has an important effect on sudden cardiac death and arrhythmia. Our study results suggest that electrocardiographic monitoring may be useful in patients with FMF

The Effect of Corrected Inflammation, Oxidative Stress and Endothelial Dysfunction on Fmd Levels in Patients with Selected Chronic Diseases: A Quasi-Experimental Study.

While the pathophysiology of chronic disorders varies there are three basic mechanisms - inflammation, oxidative stress and endothelial dysfunction - that are common in many chronic diseases. However, the failure of these mechanisms to work synchronously can lead to morbidity complicating the course of many chronic diseases. We analyzed data of 178 patients from cohorts with selected chronic diseases in this quasi-experimental study. Endothelial dysfunction was determined by flow-mediated dilatation (FMD) and asymmetric dimethylarginine (ADMA) levels. Serum ADMA, high sensitive C-reactive protein (hs-CRP), serum PTX3, malondialdehyde (MDA), Cu/Zn-superoxide dismutase (Cu/Zn-SOD), glutathione peroxidase (GSH-Px) levels and FMD were studied in baseline and after 12 weeks of Morinda citrifolia (anti-atherosclerotic liquid- AAL), omega-3 (anti-inflammatory capsules- AIC) and extract with Alaskan blueberry (anti-oxidant liquid- AOL). Stepwise multivariate regression analysis was used to evaluate the association of FMD with clinical and serologic parameters. Serum ADMA, MDA, PTX3, hsCRP and albumin levels, and proteinuria were significantly decreased while CuZn-SOD, GSH-Px and FMD levels were significantly increased following AAL, AIC and AOL therapies. The FMD was negatively correlated with serum ADMA, MDA, PTX3, and hsCRP levels and positively correlated with CuZn-SOD and eGFR levels. ADMA and PTX3 levels were independently related to FMD both before and after AAL, AIC and AOL therapies. Our study shows that serum ADMA, MDA, PTX3 levels are associated with endothelial dysfunction in patients with selected chronic diseases. In addition, short-term AAL, AIC and AOL therapies significantly improves a number of parameters in our cohort and can normalize ADMA, PTX3, hsCRP and MDA levels

FRI0547 The Effect of Corrected Inflammation, Oxidative Stress and Endothelial Dysfunction on Fmd Levels in Patients with Selected Chronic Diseases: A Quasi-Experimental Study.

While the pathophysiology of chronic disorders varies there are three basic mechanisms - inflammation, oxidative stress and endothelial dysfunction - that are common in many chronic diseases. However, the failure of these mechanisms to work synchronously can lead to morbidity complicating the course of many chronic diseases. We analyzed data of 178 patients from cohorts with selected chronic diseases in this quasi-experimental study. Endothelial dysfunction was determined by flow-mediated dilatation (FMD) and asymmetric dimethylarginine (ADMA) levels. Serum ADMA, high sensitive C-reactive protein (hs-CRP), serum PTX3, malondialdehyde (MDA), Cu/Zn-superoxide dismutase (Cu/Zn-SOD), glutathione peroxidase (GSH-Px) levels and FMD were studied in baseline and after 12 weeks of Morinda citrifolia (anti-atherosclerotic liquid- AAL), omega-3 (anti-inflammatory capsules- AIC) and extract with Alaskan blueberry (anti-oxidant liquid- AOL). Stepwise multivariate regression analysis was used to evaluate the association of FMD with clinical and serologic parameters. Serum ADMA, MDA, PTX3, hsCRP and albumin levels, and proteinuria were significantly decreased while CuZn-SOD, GSH-Px and FMD levels were significantly increased following AAL, AIC and AOL therapies. The FMD was negatively correlated with serum ADMA, MDA, PTX3, and hsCRP levels and positively correlated with CuZn-SOD and eGFR levels. ADMA and PTX3 levels were independently related to FMD both before and after AAL, AIC and AOL therapies. Our study shows that serum ADMA, MDA, PTX3 levels are associated with endothelial dysfunction in patients with selected chronic diseases. In addition, short-term AAL, AIC and AOL therapies significantly improves a number of parameters in our cohort and can normalize ADMA, PTX3, hsCRP and MDA levels

The Turkish Version of the Juvenile Arthritis Multidimensional Assessment Report (JAMAR)

The Juvenile Arthritis Multidimensional Assessment Report (JAMAR) is a new parent/patient reported outcome measure that enables a thorough assessment of the disease status in children with juvenile idiopathic arthritis (JIA). We report the results of the cross-cultural adaptation and validation of the parent and patient versions of the JAMAR in the Turkish language. The reading comprehension of the questionnaire was tested in 10 JIA parents and patients. Each participating centre was asked to collect demographic, clinical data and the JAMAR in 100 consecutive JIA patients or all consecutive patients seen in a 6-month period and to administer the JAMAR to 100 healthy children and their parents. The statistical validation phase explored descriptive statistics and the psychometric issues of the JAMAR: the 3 Likert assumptions, floor/ceiling effects, internal consistency, Cronbach\u27s alpha, interscale correlations, test-retest reliability, and construct validity (convergent and discriminant validity). A total of 466 JIA patients (13.7% systemic, 40.6% oligoarticular, 22.5% RF negative poly-arthritis, and 23.2% other categories) and 93 healthy children were enrolled in four centres. The JAMAR components discriminated well-healthy subjects from JIA patients. All JAMAR components revealed good psychometric performances. In conclusion, the Turkish version of the JAMAR is a valid tool for the assessment of children with JIA and is suitable for use both in routine clinical practice and clinical research

Effects of hospital facilities on patient outcomes after cancer surgery: an international, prospective, observational study

Background Early death after cancer surgery is higher in low-income and middle-income countries (LMICs) compared with in high-income countries, yet the impact of facility characteristics on early postoperative outcomes is unknown. The aim of this study was to examine the association between hospital infrastructure, resource availability, and processes on early outcomes after cancer surgery worldwide.Methods A multimethods analysis was performed as part of the GlobalSurg 3 study-a multicentre, international, prospective cohort study of patients who had surgery for breast, colorectal, or gastric cancer. The primary outcomes were 30-day mortality and 30-day major complication rates. Potentially beneficial hospital facilities were identified by variable selection to select those associated with 30-day mortality. Adjusted outcomes were determined using generalised estimating equations to account for patient characteristics and country-income group, with population stratification by hospital.Findings Between April 1, 2018, and April 23, 2019, facility-level data were collected for 9685 patients across 238 hospitals in 66 countries (91 hospitals in 20 high-income countries; 57 hospitals in 19 upper-middle-income countries; and 90 hospitals in 27 low-income to lower-middle-income countries). The availability of five hospital facilities was inversely associated with mortality: ultrasound, CT scanner, critical care unit, opioid analgesia, and oncologist. After adjustment for case-mix and country income group, hospitals with three or fewer of these facilities (62 hospitals, 1294 patients) had higher mortality compared with those with four or five (adjusted odds ratio [OR] 3.85 [95% CI 2.58-5.75]; p<0.0001), with excess mortality predominantly explained by a limited capacity to rescue following the development of major complications (63.0% vs 82.7%; OR 0.35 [0.23-0.53]; p<0.0001). Across LMICs, improvements in hospital facilities would prevent one to three deaths for every 100 patients undergoing surgery for cancer.Interpretation Hospitals with higher levels of infrastructure and resources have better outcomes after cancer surgery, independent of country income. Without urgent strengthening of hospital infrastructure and resources, the reductions in cancer-associated mortality associated with improved access will not be realised

Relationships between antioxidant enzymes and physiological variations occur during ageing of pepper seeds

Physiological and biochemical changes occur at different viability levels of pepper seeds. For this purpose 80, 60, 40, and 20% viability levels were obtained by controlled deterioration treatments in the seeds of 3 pepper cultivars, 'Demre Sivri', 'Kandil Dolma', and 'Yalova Carliston'. Subsequently, mean germination time (MGT), electrical conductivity (EC), catalase (CAT), peroxidase (POX), superoxide dismutase (SOD), total lipid rate (TLR) and malondialdehyde (MDA) were measured at these viability levels. Depending on ageing, MGT, EC, and MDA contents increased in the seeds of each cultivar. The increases in MGT, EC, and MDA contents were more evident when seed viability decreased to a 60% level. Moreover, a negative correlation was found between the loss of seed viability and MGT, MDA, and EC. However, there was a positive correlation between loss of seed viability and CAT, SOD, POX, and TLR in all cultivars. Therefore, it was possible to predict the germination rate and mean germination time of the pepper seeds after detection of their antioxidant enzymes