Structure and function of natural sulphide-oxidizing microbial mats under dynamic input of light and chemical energy

We studied the interaction between phototrophic and chemolithoautotrophic sulphide-oxidizing microorganisms in natural microbial mats forming in sulphidic streams. The structure of these mats varied between two end-members: one characterized by a layer dominated by large sulphur-oxidizing bacteria (SOB; mostly Beggiatoa-like) on top of a cyanobacterial layer (B/C mats) and the other with an inverted structure (C/B mats). C/B mats formed where the availability of oxygen from the water column was limited (<5 mu M). Aerobic chemolithotrophic activity of the SOB depended entirely on oxygen produced locally by cyanobacteria during high light conditions. In contrast, B/C mats formed at locations where oxygen in the water column was comparatively abundant (445 mu M) and continuously present. Here SOB were independent of the photosynthetic activity of cyanobacteria and outcompeted the cyanobacteria in the uppermost layer of the mat where energy sources for both functional groups were concentrated. Outcompetition of photosynthetic microbes in the presence of light was facilitated by the decoupling of aerobic chemolithotrophy and oxygenic phototrophy. Remarkably, the B/C mats conserved much less energy than the C/B mats, although similar amounts of light and chemical energy were available. Thus ecosystems do not necessarily develop towards optimal energy usage. Our data suggest that, when two independent sources of energy are available, the structure and activity of microbial communities is primarily determined by the continuous rather than the intermittent energy source, even if the time-integrated energy flux of the intermittent energy source is greater

Continuum theory of tilted chiral smectic phases

We demonstrate that the sequence of distorted commensurate phases observed in tilted chiral smectics is explained by the gain in electrostatic energy due to the lock-in of the unit cell to a number of layers which is the integer closest to the ratio pitch over thickness of the subjacent Sm-C$^*_\alpha$ phase. We also explain the sign change of the helicity in the middle of the sequence by a balance between two twist sources one intrinsic and another due to the distortion of the Sm-C$^*_\alpha$

Shock recovery experiments confirm the possibility of transferring viable microorganisms from Mars to Earth

Extract from introduction: With regard to the impact and ejection phase we tested the case for the transfer of microorganisms from Mars to Earth. Using a high explosive set-up thin layers of bacterial endospores of Bacillus subtilis, of the lichen Xanthoria elegans and of the cyanobacterium Chroococcidiopsis sp. embedded between two plates of gabbro were subjected to 10, 20, 30, 40 and 50 GPa which is the pressure range observed in Martian meteorites [1]

Spacetime Emergence in the Robertson-Walker Universe from a Matrix model

Using a novel, string theory-inspired formalism based on a Hamiltonian constraint, we obtain a conformal mechanical system for the spatially flat four-dimensional Robertson-Walker Universe. Depending on parameter choices, this system describes either a relativistic particle in the Robertson-Walker background, or metric fluctuations of the Robertson-Walker geometry. Moreover we derive a tree-level M-theory matrix model in this time-dependent background. Imposing the Hamiltonian constraint forces the spacetime geometry to be fuzzy near the big bang, while the classical Robertson-Walker geometry emerges as the Universe expands. From our approach we also derive the temperature of the Universe interpolating between the radiation and matter dominated eras.Comment: 4 pages - accepted for publication in Physical Review Letter

Life after shock: the mission from Mars to Earth

Extract from introduction: The minerals of the Martian meteorites collected so far indicate an exposure to shock waves in the pressure range of 5 to 55 GPa [1]. As terrestrial rocks are frequently inhabited by microbial communities, rocks ejected from a planet by impact processes may carry with them endolithic microorganisms, if microbial life existed/exists on this planet

Innovation intermediaries and collaboration: Knowledge–based practices and internal value creation

This paper analyses how knowledge-based practices adopted by innovation intermediaries enable them to generate value for themselves when collaborating with their clients. While the literature focuses on value creation for their client organisations, little is known about how innovation intermediaries create internal value even though this is essential for ensuring their long-term survival and sustaining their key facilitating role in the innovation system. This understudied issue is explored using empirical evidence from a sub-set of innovation intermediaries, Research and Technology Organisations (RTOs). The results indicate that by capitalising on existing knowledge vested in employees and collaborators as well as understanding and shaping the knowledge base of the innovation ecosystem, innovation intermediaries generate internal value from their involvement in collaborative innovation, which range from different financial to non-financial types of value. Implications for intermediaries, their collaborators and for policymakers are then discussed

The perceived service quality, satisfaction and behavioural intent towards cellphone network service providers : a generational perspective

The importance of providing customers with quality services in order to satisfy them and encourage future purchases is well documented in literature. Yet, the cellphone network provider industry of South Africa seems to be riddled with service quality problems. This study aims to determine different generations’ perceived service quality of services and satisfaction levels with services provided by cellphone network service providers, as well as their behavioural intentions towards these providers. The study furthermore examines the correlations between these three constructs and uncovers significant differences between different generations. Data was collected from 2339 respondents by means of a self-administered questionnaire. Young Generation Y consumers perceive the service quality levels and service satisfaction levels of these providers as significantly lower than other generations. This is also true for their behavioural intent. Significant correlations also exist between the generations’ perceived level of service quality they experience, their perceived level of satisfaction with, and their behavioural intent towards providers. This implies that providers should strongly focus their efforts on satisfying the needs, and improving the service satisfaction of young Generation Y consumers in order to retain them in the future. Providers should also vary marketing strategies based upon the differences uncovered between the generations

The polymeric stability of the Escherichia coli F4 (K88) fimbriae enhances its mucosal immunogenicity following oral immunization

&lt;p&gt;Only a few vaccines are commercially available against intestinal infections since the induction of a protective intestinal immune response is difficult to achieve. For instance, oral administration of most proteins results in oral tolerance instead of an antigen-specific immune response. We have shown before that as a result of oral immunization of piglets with F4 fimbriae purified from pathogenic enterotoxigenic Escherichia coli (ETEC), the fimbriae bind to the F4 receptor (F4R) in the intestine and induce a protective F4-specific immune response. F4 fimbriae are very stable polymeric structures composed of some minor subunits and a major subunit FaeG that is also the fimbrial adhesin. In the present study, the mutagenesis experiments identified FaeG amino acids 97 (N to K) and 201 (I to V) as determinants for F4 polymeric stability. The interaction between the FaeG subunits in mutant F4 fimbriae is reduced but both mutant and wild type fimbriae behaved identically in F4R binding and showed equal stability in the gastro-intestinal lumen. Oral immunization experiments indicated that a higher degree of polymerisation of the fimbriae in the intestine was correlated with a better F4-specific mucosal immunogenicity. These data suggest that the mucosal immunogenicity of soluble virulence factors can be increased by the construction of stable polymeric structures and therefore help in the development of effective mucosal vaccines.&lt;/p&gt;</p

Impact experiments in support of “Lithopanspermia”: The route from Mars to Earth

Shock recovery experiments on a Martian analogue rock (gabbro) loaded with three types of microorganisms reveal that these organisms survive the impact and ejection phase on Mars at shock pressures up to about 50 GPa with exponentially decreasing survival rates