Ochratoxigenic Black Species of Aspergilli in Grape Fruits of Northern Italy Identified by an Improved PCR-RFLP Procedure

A collection of 356 isolates of Aspergillus spp. collected during 2006 and 2007 from grapevines in northern Italy were identified through Internal Transcribed Spacer based Restriction Fragment Length Polymorphism (ITS-RFLP) and tested for ochratoxin A (OTA) production. Restriction endonuclease digestion of the ITS products using the endonucleases HhaI, HinfI and RsaI, distinguished five different RFLPs. From each pattern, three samples were sequenced and the nucleotide sequences showed different species corresponding to Aspergillus niger, A. carbonarius, A. tubingensis, A. japonicus and A. aculeatus. By comparing the sequences of the ITS regions, also the uniseriate species A. japonicus and A. aculeatus could be differentiated by HinfI digestion of the ITS products. Among the aspergilli, A. niger was the major species associated with grapes during 2006 (57.4%), while A. carbonarius was the major species during 2007 (46.6%). All the strains of Aspergillus were tested for their ability to produce OTA on Yeast extract sucrose medium (YES), as it was tested as an optimal substrate for the evaluation of OTA production by black aspergilli. Out of 356 isolates, 63 (17.7%) isolates produced OTA ranging from 0.05 to 3.0 µg mL−1. Most of the ochratoxigenic isolates were A. carbonarius (46) in both years, but also some strains of A. tubingensis (11) and A. japonicus (6) produced lower amounts of OTA

Comparing extrapolations of the coronal magnetic field structure at 2.5 solar radii with multi-viewpoint coronagraphic observations

The magnetic field shapes the structure of the solar corona but we still know little about the interrelationships between the coronal magnetic field configurations and the resulting quasi-stationary structures observed in coronagraphic images (as streamers, plumes, coronal holes). One way to obtain information on the large-scale structure of the coronal magnetic field is to extrapolate it from photospheric data and compare the results with coronagraphic images. Our aim is to verify if this comparison can be a fast method to check systematically the reliability of the many methods available to reconstruct the coronal magnetic field. Coronal fields are usually extrapolated from photospheric measurements typically in a region close to the central meridian on the solar disk and then compared with coronagraphic images at the limbs, acquired at least 7 days before or after to account for solar rotation, implicitly assuming that no significant changes occurred in the corona during that period. In this work, we combine images from three coronagraphs (SOHO/LASCO-C2 and the two STEREO/SECCHI-COR1) observing the Sun from different viewing angles to build Carrington maps covering the entire corona to reduce the effect of temporal evolution to ~ 5 days. We then compare the position of the observed streamers in these Carrington maps with that of the neutral lines obtained from four different magnetic field extrapolations, to evaluate the performances of the latter in the solar corona. Our results show that the location of coronal streamers can provide important indications to discriminate between different magnetic field extrapolations.Comment: Accepted by A&A the 20th of May, 201

Restoration in optical cloud networks with relocation and services differentiation

©2016 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.Optical cloud networks allow for the integrated management of both optical and IT resources. In this paradigm, cloud services can be provisioned in an anycast fashion; i.e., only the source node asking for a service is specified, while it is up to the cloud control/management system to select the most suitable destination data center (DC) node. During the cloud service provisioning process, resiliency is crucial in order to guarantee continuous network operations also in the presence of failures. On the one hand, a survivability strategy needs to be able to meet the availability requirements of each specific cloud service, while on the other hand it must be efficient in using backup resources. This paper proposes a restoration-based survivability strategy, which combines the benefits of both cloud service relocation and service differentiation concepts. The former is used to enhance the restorability performance (i.e., the percentage of successfully restored cloud services) offered by restoration, while the latter ensures that critical services are given the proper consideration while backup resources are assigned. The paper proposes both an integer linear programming (ILP) formulation, which guarantees optimal results, and a heuristic, which trades the optimality of the solution achieved by the ILP for faster processing times. Simulation results show that the average service availability and restorability performance obtained by both the ILP and the heuristic are very close to that achievable using a protection-based strategy, but with the inherent benefit, in terms of efficient use of resources, offered by a restoration-based approach.Peer ReviewedPostprint (author's final draft

Seasonality Role on the Phenolics from Cultivated Baccharis dracunculifolia

Baccharis dracunculifolia is the source of Brazilian green propolis (BGP). Considering the broad spectrum of biological activities attributed to green proplis, B. dracunculifolia has a great potential for the development of new cosmetic and pharmaceutical products. In this work, the cultivation of 10 different populations of native B. dracunculifolia had been undertaken aiming to determine the role of seasonality on its phenolic compounds. For this purpose, fruits of this plant were collected from populations of 10 different regions, and 100 individuals of each population were cultivated in an experimental area of 1800 m2. With respect to cultivation, the yields of dry plant, essential oil and crude extract were measured monthly resulting in mean values of 399 ± 80 g, 0.6 ± 0.1% and 20 ± 4%, respectively. The HPLC analysis allowed detecting seven phenolic compounds: caffeic acid, ferulic acid, aromadendrin-4′-methyl ether (AME), isosakuranetin, artepillin C, baccharin and 2-dimethyl-6-carboxyethenyl-2H-1-benzopyran acid, which were the major ones throughout the 1-year monthly analysis. Caffeic acid was detected in all cultivated populations with mean of 4.0%. AME displayed the wide variation in relation to other compounds showing means values of 0.65 ± 0.13% at last quarter. Isosakuranetin and artepillin C showed increasing concentrations with values between 0% and 1.4% and 0% and 1.09%, respectively. The obtained results allow suggesting that the best time for harvesting this plant, in order to obtain good qualitative and quantitative results for these phenolic compounds, is between December and April

Slow wind belt in the quiet solar corona

The slow solar wind belt in the quiet corona, observed with the Metis coronagraph on board Solar Orbiter on May 15, 2020, during the activity minimum of the cycle 24, in a field of view extending from 3.8 $R_\odot$ to 7.0 $R_\odot$, is formed by a slow and dense wind stream running along the coronal current sheet, accelerating in the radial direction and reaching at 6.8 $R_\odot$ a speed within 150 km s$^{-1}$ and 190 km s$^{-1}$, depending on the assumptions on the velocity distribution of the neutral hydrogen atoms in the coronal plasma. The slow stream is separated by thin regions of high velocity shear from faster streams, almost symmetric relative to the current sheet, with peak velocity within 175 km s$^{-1}$ and 230 km s$^{-1}$ at the same coronal level. The density-velocity structure of the slow wind zone is discussed in terms of the expansion factor of the open magnetic field lines that is known to be related to the speed of the quasi-steady solar wind, and in relation to the presence of a web of quasi separatrix layers, S-web, the potential sites of reconnection that release coronal plasma into the wind. The parameters characterizing the coronal magnetic field lines are derived from 3D MHD model calculations. The S-web is found to coincide with the latitudinal region where the slow wind is observed in the outer corona and is surrounded by thin layers of open field lines expanding in a non-monotonic way

In-flight validation of Metis Visible-light Polarimeter Coronagraph on board Solar Orbiter

Context. The Metis coronagraph is one of the remote-sensing instruments of the ESA/NASA Solar Orbiter mission. Metis is aimed at the study of the solar atmosphere and solar wind by simultaneously acquiring images of the solar corona at two different wavelengths; visible-light (VL) within a band ranging from 580 nm to 640 nm, and in the HI Ly-alpha 121.6 +/- 10 nm ultraviolet (UV) light. The visible-light channel includes a polarimeter with electro-optically modulating Liquid Crystal Variable Retarders (LCVRs) to measure the linearly polarized brightness of the K-corona to derive the electron density. Aims. In this paper, we present the first in-flight validation results of the Metis polarimetric channel together with a comparison to the on-ground calibrations. It is the validation of the first use in deep space (with hard radiation environment) of an electro-optical device: a liquid crystal-based polarimeter. Methods. We used the orientation of the K-corona's linear polarization vector during the spacecraft roll maneuvers for the in-flight calibration. Results. The first in-flight validation of the Metis coronagraph on-board Solar Orbiter shows a good agreement with the on-ground measurements. It confirms the expected visible-light channel polarimetric performance. A final comparison between the first pB obtained by Metis with the polarized brightness (pB) obtained by the space-based coronagraph LASCO and the ground-based coronagraph KCor shows the consistency of the Metis calibrated results.Comment: 8 pages, 13 figures, 3 tables, pape

Connecting Solar Orbiter remote-sensing observations and Parker Solar Probe in situ measurements with a numerical MHD reconstruction of the Parker spiral

As a key feature, NASA’s Parker Solar Probe (PSP) and ESA-NASA’s Solar Orbiter (SO) missions cooperate to trace solar wind and transients from their sources on the Sun to the inner interplanetary space. The goal of this work is to accurately reconstruct the interplanetary Parker spiral and the connection between coronal features observed remotely by the Metis coronagraph on-board SO and those detected in situ by PSP at the time of the first PSP-SO quadrature of January 2021. We use the Reverse in situ and MHD Approach (RIMAP), a hybrid analytical-numerical method performing data-driven reconstructions of the Parker spiral. RIMAP solves the MHD equations on the equatorial plane with the PLUTO code, using the measurements collected by PSP between 0.1 and 0.2 AU as boundary conditions. Our reconstruction connects density and wind speed measurements provided by Metis (3–6 solar radii) to those acquired by PSP (21.5 solar radii) along a single streamline. The capability of our MHD model to connect the inner corona observed by Metis and the super Alfvénic wind measured by PSP, not only confirms the research pathways provided by multi-spacecraft observations, but also the validity and accuracy of RIMAP reconstructions as a possible test bench to verify models of transient phenomena propagating across the heliosphere, such as coronal mass ejections, solar energetic particles and solar wind switchbacks

Recurrent solar density transients in the slow wind observed with the Metis coronagraph

Aims We aim to investigate and characterize the morphology and dynamics of small-scale coronal plasma density inhomogeneities detected as brighter, denser features propagating outward through the solar corona in the visible-light images of the Metis coronagraph on board Solar Orbiter on February 22, 2021. Our main focus is on investigating their possible origin and contribution to the slow wind variability and dynamics and their dependence on coronal magnetic field configurations and structure. Methods. The method adopted is based on the computations of autocorrelation and cross-correlation functions applied to temporal and spatial series of total brightness as a function of the heliocentric distance and solar latitudes. Results. We find that the plasma density inhomogeneities studied here are small-scale structures with typical radial and transverse sizes, as projected on the plane of sky, on the order of 500 Mm and 40 Mm, respectively, and that they are up to 24 times brighter than the ambient solar wind. The brighter density structures exhibit longer lifetime and more stable shape and dimensions as they travel toward the outer edge of the field of view. The enhanced density structures are ejected with a most probable cadence of about 80 min at or below the inner edge of the Metis field of view (within 3.1 R· 5.7 R· at the time of observations) in a wide latitudinal region corresponding to the site of a complex web of separatrix and quasi-separatrix layers, as resulting from the simulated magnetohydrodynamic configuration of the west limb of the solar corona. Some of the moving density enhancements clearly show morphological characteristics compatible with the switchback phenomenon, supporting the results indicating that the switchbacks occur at the coronal level. The enhanced density structures were ejected into the ambient slow wind with a mean velocity of about 240 ± 40 km s-1, which is significantly higher than that deduced for the ambient solar wind on the basis of previous Metis observations during the solar minimum of cycle 24. The absence of acceleration observed across the coronagraph field of view suggests that the ejected plasmoids are progressively reaching the expansion rate of the ambient wind. Conclusions. The results suggest that the quasi-periodic enhanced-density plasmoids might be the consequence of reconnection phenomena occurring in the complex web of the separatrix and quasi-separatrix layers present in the solar corona. Moreover, the structural characteristics of some of the detected plasmoids are in favor of the presence of switchbacks that originate during interchange reconnection processes occurring at or below 3 R· in the S-web. The speed of the plasma ejected in the reconnection process is higher than that of the ambient slow solar wind and is likely to be related to the energy involved in the process generating the propagating structures

Particle monitoring capability of the Solar Orbiter Metis coronagraph through the increasing phase of solar cycle 25

Context. Galactic cosmic rays (GCRs) and solar particles with energies greater than tens of MeV penetrate spacecraft and instruments hosted aboard space missions. The Solar Orbiter Metis coronagraph is aimed at observing the solar corona in both visible (VL) and ultraviolet (UV) light. Particle tracks are observed in the Metis images of the corona. An algorithm has been implemented in the Metis processing electronics to detect the VL image pixels crossed by cosmic rays. This algorithm was initially enabled for the VL instrument only, since the process of separating the particle tracks in the UV images has proven to be very challenging. Aims. We study the impact of the overall bulk of particles of galactic and solar origin on the Metis coronagraph images. We discuss the effects of the increasing solar activity after the Solar Orbiter mission launch on the secondary particle production in the spacecraft. Methods. We compared Monte Carlo simulations of GCRs crossing or interacting in the Metis VL CMOS sensor to observations gathered in 2020 and 2022. We also evaluated the impact of solar energetic particle events of different intensities on the Metis images. Results. The study of the role of abundant and rare cosmic rays in firing pixels in the Metis VL images of the corona allows us to estimate the efficiency of the algorithm applied for cosmic-ray track removal from the images and to demonstrate that the instrument performance had remained unchanged during the first two years of the Solar Orbiter operations. The outcome of this work can be used to estimate the Solar Orbiter instrument's deep charging and the order of magnitude for energetic particles crossing the images of Metis and other instruments such as STIX and EUI.Comment: 8 pages, 6 figure