Genomic exploration of sequential clinical isolates reveals a distinctive molecular signature of persistent Staphylococcus aureus bacteraemia.

Large-scale genomic studies of within-host diversity in Staphylococcus aureus bacteraemia (SAB) are needed to understanding bacterial adaptation underlying persistence and thus refining the role of genomics in management of SAB. However, available comparative genomic studies of sequential SAB isolates have tended to focus on selected cases of unusually prolonged bacteraemia, where secondary antimicrobial resistance has developed. To understand bacterial genetic diversity during SAB more broadly, we applied whole genome sequencing to a large collection of sequential isolates obtained from patients with persistent or relapsing bacteraemia. After excluding genetically unrelated isolates, we performed an in-depth genomic analysis of point mutations and chromosome structural variants arising within individual SAB episodes. We show that, while adaptation pathways are heterogenous and episode-specific, isolates from persistent bacteraemia have a distinctive molecular signature, characterised by a low mutation frequency and high proportion of non-silent mutations. Analysis of structural genomic variants revealed that these often overlooked genetic events are commonly acquired during SAB. We discovered that IS256 insertion may represent the most effective driver of within-host microevolution in selected lineages, with up to three new insertion events per isolate even in the absence of other mutations. Genetic mechanisms resulting in significant phenotypic changes, such as increases in vancomycin resistance, development of small colony phenotypes, and decreases in cytotoxicity, included mutations in key genes (rpoB, stp, agrA) and an IS256 insertion upstream of the walKR operon. This study provides for the first time a large-scale analysis of within-host genomic changes during invasive S. aureus infection and describes specific patterns of adaptation that will be informative for both understanding S. aureus pathoadaptation and utilising genomics for management of complicated S. aureus infections

Whipple's Disease With Neurological Manifestations: Case Report

Whipple's disease (WD) is an uncommon multisystem condition caused by the bacillus Tropheryma whipplei. Central nervous system involvement is a classical feature of the disease observed in 20 to 40% of the patients. We report the case of a 62 yeards old man with WD that developed neurological manifestations during its course, and discuss the most usual signs and symptoms focusing on recent diagnostic criteria and novel treatment regimens.622 A342346Whipple, G.H., A hitherto undescribed disease characterized anatomically by deposits of fat and fatty acids in the intestinal and mesenteric lymphatic tissues (1907) Johns Hopkins Hosp Bull, 18, pp. 382-391Marth, T., Raoult, D., Whipple's disease (2003) Lancet, 36, pp. 239-246Gerard, A., Sarrot-Reynauld, F., Liozon, E., Neurologic presentation of Whipple disease: Report of 12 cases and review of the literature (2002) Medicine (Baltimore), 81, pp. 443-457Brown, A.P., Lane, J.C., Murayama, S., Vollmer, D.G., Whipple's disease presenting with isolated neurological symptoms: Case report (1990) J Neurosurg, 73, pp. 623-627Bostwick, D.G., Bensch, K.G., Burke, J.S., Whipple's disease presenting as aortic insufficiency (1981) N Engl J Med, 305, pp. 995-998Raoult, D., A febrile, blood culture-negative endocarditis (1999) Ann Intern Med, 131, pp. 144-146Chan, R.Y., Yannuzzi, L.A., Foster, C.S., Ocular Whipple's disease: Earlier definitive diagnosis (2001) Ophthalmology, 108, pp. 2225-2231Louis, E.D., Lynch, T., Kaufmann, P., Fahn, S., Odel, J., Diagnostic guidelines in central nervous system Whipple's disease (1996) Ann Neurol, 40, pp. 561-568Sieracki, J.C., Whipple's disease: Observations on systemic involvement (1958) Amer Med Asso Arch Pathol, 66, pp. 464-467Anderson, M., Neurology of Whipple's disease (2000) J Neurol Neurosurg Psychiatry, 68, pp. 2-5De Coene, B., Gilliard, C., Indekeu, P., Whipple's disease confined to the central nervous system (1996) Neuroradiology, 38, pp. 325-327Verhagen, W.I.M., Huygen, P.L.M., Dalman, J.E., Schuurmans, M.M.J., Whipple's disease and the central nervous system: A case report and a review of the literature (1996) Clin Neurol Neurosurg, 98, pp. 299-304Feldman, M., Hendler, R.S., Morrison, E.B., Acute meningoencephalitis after withdrawal of antibiotics in Whipple's disease (1980) Ann Intern Med, 93, pp. 709-711Schwartz, M.A., Selhorst, J.B., Ochs, A.L., Oculomasticatory myorhythmia: A unique movement disorder occurring in Whipple's disease (1986) Ann Neurol, 20, pp. 677-683Manzel, K., Tranel, D., Cooper, G., Cognitive and behavioral abnormalities in a case of central nervous system Whipple disease (2000) Arch Neurol, 57, pp. 399-403Halperin, J.J., Landis, D.M., Kleinman, G.M., Whipple's disease of the nervous system (1982) Neurology, 32, pp. 612-617Feurle, G.E., Volk, B., Waldherr, R., Cerebral Whipple's disease with negative jejunal histology (1979) N Engl J Med, 300, pp. 907-908Madoule, P., Ciaudio-Lacroix, C., Halimi, P., Osteoarticular lesions in Whipple's disease, a propos of a destructive form and review of the literature (1985) J Radiol, 66, pp. 345-350Brändle, M., Ammann, P., Spinas, G.A., Relapsing Whipple's disease presenting with hypopituitarism (1999) Clin Endocrinol, 50, pp. 399-403Topper, R., Gartung, C., Block, F., Neurologic complications in inflammatory bowel diseases (2002) Nervenarzt, 73, pp. 489-499Clarke, C.E., Falope, Z.F., Abdelhadi, H.A., Cervical myelopathy caused by Whipple's disease (1998) Neurology, 50, pp. 1505-1506Ramzan, N.N., Loftus, E., Burgart, L.J., Diagnosis and monitoring of Whipple disease by polymerase chain reaction (1997) Ann Intern Med, 126, pp. 520-527Von Herbay, A., Ditton, H.J., Scuhmacher, F., Whipple's disease: Staging and monitoring by cytology and polymerase chain reaction analysis of cerebrospinal fluid (1997) Gastroenterology, 113, pp. 434-441Kremer, S., Besson, G., Bonaz, B., Pasquier, B., Le Bas, J.F., Grand, S., Diffuse lesions in the CNS revealed by MR imaging in a case of Whipple disease (2001) Am J Neuroradiol, 22, pp. 493-495Romanul, F.C., Radvany, J., Rosales, R.K., Whipple's disease confined to the brain: A case studied clinically and pathologically (1977) J Neurol Neurosurg Psychiatry, 40, pp. 901-909Thompson, D.G., Leidingham, J.M., Howard, A.J., Brown, C.L., Meningitis in Whipple's disease (1978) BMJ, 2, pp. 14-15Feurle, G.E., Marth, T., An evaluation of antimicrobial treatment for Whipple's disease: Tetracycline versus trimethoprim-sulfamethoxazole (1994) Dig Dis Sci, 39, pp. 1642-1648Misbah, S.A., Mapstone, N.P., Whipple's disease revisited (2000) J Clin Pathol, 53, pp. 750-755Schnider, P.J., Reisinger, E.C., Berger, T., Krejs, G.J., Auff, E., Treatment guidelines in central nervous system Whipple's disease (1997) Ann Neurol, 41, pp. 561-56

DIAGNÓSTICO AMBIENTAL DO RIO PARAGUAI EM CÁCERES A PARTIR DE INDICADORES MORFOLÓGICOS, PANTANAL SUPERIOR – BRASIL

O objetivo deste estudo foi verificar o nível de degradação do rio Paraguai a partir de indicadores morfológicos e tipologias de uso, no trecho do perímetro urbano de Cáceres – Mato Grosso, bem como nas áreas de influência. Para tanto, os dados foram obtidos através dos Protocolos de Avaliação Rápida de Rios, adaptados de Callisto et al. (2002) e Rodrigues et al. (2012). Para análise granulométrica dos sedimentos adotou-se Embrapa (1997), Suguio (1973), Carvalho (2000) e Leli et al. (2010)

Muscular Strength Imbalances Are not Associated with Skin Temperature Asymmetries in Soccer Players

Although strength imbalances using isokinetic dynamometer have been examined for injury risk screening in soccer players, it is very expensive and time-consuming, making the evaluation of new methods appealing. The aim of the study was to analyze the agreement between muscular strength imbalances and skin temperature bilateral asymmetries as well as skin temperature diferences in the hamstrings and quadriceps. The skin temperature of the anterior and posterior thigh of 59 healthy male soccer athletes was assessed at baseline using infrared thermography for the identification of hamstrings-quadriceps skin temperature diferences and thermal asymmetries (>0.5 ºC). Subsequently, concentric and eccentric peak torque of the quadriceps and hamstrings were considered in the determination of the ratios, as well as muscular asymmetries (>15%). When considering the torque parameters, 37.3% (n = 22) of the players would be classified as high risk for injuries. The percentage of those presenting skin temperature imbalances superior to 0.5 C was 52.5% (n = 31). The skin temperature assessment showed sensitivity (22%) and specificity (32.2%) to identify torque asymmetries, demonstrating the inability to identify false negatives (15.3%) and false positives (30.5%) from all soccer athletes. In conclusion, skin temperature diferences between hamstrings and quadriceps could be more related to thermoregulatory factors than strength imbalances

ANN-based energy reconstruction procedure for TACTIC gamma-ray telescope and its comparison with other conventional methods

The energy estimation procedures employed by different groups, for determining the energy of the primary $\gamma$-ray using a single atmospheric Cherenkov imaging telescope, include methods like polynomial fitting in SIZE and DISTANCE, general least square fitting and look-up table based interpolation. A novel energy reconstruction procedure, based on the utilization of Artificial Neural Network (ANN), has been developed for the TACTIC atmospheric Cherenkov imaging telescope. The procedure uses a 3:30:1 ANN configuration with resilient backpropagation algorithm to estimate the energy of a $\gamma$-ray like event on the basis of its image SIZE, DISTANCE and zenith angle. The new ANN-based energy reconstruction method, apart from yielding an energy resolution of $\sim$ 26%, which is comparable to that of other single imaging telescopes, has the added advantage that it considers zenith angle dependence as well. Details of the ANN-based energy estimation procedure along with its comparative performance with other conventional energy reconstruction methods are presented in the paper and the results indicate that amongst all the methods considered in this work, ANN method yields the best results. The performance of the ANN-based energy reconstruction has also been validated by determining the energy spectrum of the Crab Nebula in the energy range 1-16 TeV, as measured by the TACTIC telescope.Comment: 23pages, 9 figures Accepted for publication in NIM

Antiviral Activity Of Bacillus Sp. Isolated From The Marine Sponge Petromica Citrina Against Bovine Viral Diarrhea Virus, A Surrogate Model Of The Hepatitis C Virus

The Hepatitis C virus causes chronic infections in humans, which can develop to liver cirrhosis and hepatocellular carcinoma. The Bovine viral diarrhea virus is used as a surrogate model for antiviral assays for the HCV. From marine invertebrates and microorganisms isolated from them, extracts were prepared for assessment of their possible antiviral activity. Of the 128 tested, 2 were considered active and 1 was considered promising. The best result was obtained from the extracts produced from the Bacillus sp. isolated from the sponge Petromica citrina. The extracts 555 (500 μg/mL, SI>18) and 584 (150 μg/mL, SI 27) showed a percentage of protection of 98% against BVDV, and the extract 616, 90% of protection. All of them showed activity during the viral adsorption. Thus, various substances are active on these studied organisms and may lead to the development of drugs which ensure an alternative therapy for the treatment of hepatitis C. © 2013 by the authors; licensee MDPI, Basel, Switzerland.5512191230Yasuhara-Bell, J., Yang, Y., Barlow, R., Trapido-Rosental, H., Lu, Y., In vitro evaluation of marine microorganism extracts for antiviral activity (2010) Virol. J., 7, p. 182Ravikumar, Y.S., Upasana, R., Nandhitha, M., Perween, A., Naika, H.R., Inhibition of hepatitis C virus replication by herbal extract: Phyllanthus amarus as a potent natural source (2011) Virus Res., 158, pp. 89-97Li, H., Stoddard, M.B., Wang, S., Blair, L.M., Giorgi, E.E., Elucidation of Hepatitis C Virus Transmission and Early Diversification by Single Genome sequencing (2012) PLoS Pathog., 8, pp. e1002880Suzuki, T., Ishii, K., Aizaki, H., Wakita, T., Hepatitis C viral life cycle (2007) Adv. Drug Del. Rev., 59, pp. 1200-1212Barnes, E., Folgori, A., Capone, S., Swadling, L., Aston, S., Kurioka, A., Meyer, J., Klenerman, P., Novel adenovirus-based vaccines induce broad and sustained T cell responses to HCV in man (2012) SciTransl Med., 4, pp. 115ra1. , doi:10.1126/scitranslmed.3003155Sako, K., Aoyama, H., Sato, S., Hashimoto, Y., Baba, M., γ-Carboline derivatives with anti-bovine viral diarrhea virus (BVDV) activity (2008) Bioorg. Med. Chem., 16, pp. 3780-3790Martinot-Peignoux, M., Boyer, N., Pouteau, M., Castelnau, C., Giuily, N., Duchatelle, V., Aupérin, A., Erlinger, S., Predictors of sustained response to alpha interferon therapy in chronic hepatitis C (1998) J. Hepatol., 29, pp. 214-223Lemon, S.M., McKeating, J.A., Pietschmann, T., Frick, D.N., Glenn, J.S., Tellinghuisen, T.L., Symons, J., Furman, P.A., Development of novel therapies for hepatitis C (2010) Antiviral Res., 86, pp. 79-92Duverlie, G., Wychowski, C., Cell culture systems for the hepatitis C virus (2007) J. Gastroenterol., 13, pp. 2442-2445Finkielsztein, L.M., Moltrasio, G.Y., Caputto, M.E., Castro, E.F., Cavallaro, L.V., Moglioni, A.G., What is known about the antiviral agents active against bovine viral diarrhea virus (BVDV)? (2010) Curr. Med. Chem., 17, pp. 2933-2955Buckwold, V.E., Beer, B.E., Donis, R.O., Bovine viral diarrhea virus as a surrogate model of hepatitis C virus for the evaluation of antiviral agents (2003) Antiviral Res., 60, pp. 1-15Zitzmann, N., Mehta, A.S., Carrouee, S., Butters, T.D., Platt, F.M., McCauley, J., Blumberg, B.S., Block, T.M., Imino sugars inhibit the formation and secretion of bovine viral diarrhea virus, a Pestivirus model of hepatitis C virus: Implications for the development of broad spectrum anti-hepatitis virus agents (1999) Proc. Natl. Acad. Sci., 96, pp. 11878-11882Baginski, S.G., Pevear, D.C., Seipel, M., Sun, S.C., Benetatos, C.A., Chunduru, S.K., Rice, C.M., Collett, M.S., Mechanism of action of a pestivirus antiviral compound (2000) Proc. Natl. Acad. Sci., 97, pp. 7981-7986Buckwold, V.E., Wei, J., Wenzel-Mathers, M., Russell, J., Synergistic in vitro interactions between alpha interferon and ribavirin against bovine viral diarrhea virus and yellow fever virus as surrogate models of hepatitis C virus replication (2003) Antimicrobial. Agents Chemother., 47, pp. 2293-2298Yanagida, K., Baba, C., Baba, M., Inhibition of bovine viral diarrhea virus (BVDV) by mizoribine: Synergistic effect of combination with interferon-α (2004) Antiviral Res., 64, pp. 195-201Mosmann, T., Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays (1983) J. Immun. Meth., 65, pp. 55-63Scudiero, D.A., Shoemaker, R.H., Paull, K.D., Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines (1988) Cancer Res., 48, pp. 4827-4833Reed, L.J., Münch, H.A., A simple method of estimating fifty percent endpoints (1938) Am. J. Hyg., 27, pp. 493-497Takeuchi, H., Baba, M., Shigeta, S., An application of tetrazolium (MTT) colorimetric assay for the screening of anti-herpes simplex virus compounds (1991) J. Virol. Methods., 33, pp. 61-71Koseki, I., Simoni, I.C., Nakamura, I.T., Noronha, A.B., Costa, S.S., Antiviral activity of plant extracts against aphtovirus, pseudorabiesvírus and pestivirus in cell cultures (1990) Microbios. Letters., 44, pp. 19-30Pospiech, A., Neumann, B., A versatile quick-prep of genomic DNA from Gram-positive bacteria (1995) Trends Genetics, 11, pp. 217-218Lane, D.J., Pace, B., Olsen, G.J., Stahl, D.A., Sogin, M.L., Pace, N.R., Rapid determination of 16S ribosomal RNA sequences for phylogenetic analysis (1985) Proc. Nat. Acad. Sci., 82, pp. 6955-6959Menezes, C.B.A., Bonugli-Santos, R.C., Miqueletto, P.B., Passarini, M.R.Z., Silva, C.H.D., Justo, M.R., Rebeca, R., Sette, L.D., Microbial diversity associated with algae, ascidians and sponges from the north coast of São Paulo state, Brazil (2010) Microbiol. Res., 165, pp. 466-482Costa-Lotufo, L.V., Wilke, D.V., Jimenez, P.C., Epifanio, R.A., Organismos marinhos como fonte de novos fármacos: Histórico & perspectivas (2009) Quim. Nova., 32, pp. 703-716Laport, M.S., Santos, O.C., Muricy, G., Marine sponges: Potential sources of new antimicrobial drugs (2009) Curr. Pharm. Biotechnol., 10, pp. 86-105Marinho, P.R., Muricy, G.R.S., Silva, M.F.L., de Marval, M.G., Laport, M.S., Antibiotic-resistant bacteria inhibited by extracts and fractions from Brazilian marine sponges (2010) Brazilian J. Pharmac., 20, pp. 267-275Donadio, S., Monciardini, P., Sosio, M., Polyketide synthases and nonribosomal peptide synthetases: The emerging view from bacterial genomics (2007) Nat. Prod. Rep., 24, pp. 1073-1109Mikhaǐlova, N.A., Nagieva, F.G., Grin'ko, O.M., Zverev, V.V., Experimental study of antiviral activity of spore-forming bacterium Bacillus pumilus "Pashkov" (2010) Mikrobiol. Epidemiol. Immunobiol., 2, pp. 69-74Gribencha, S.V., Potselueva, L.A., Barinskiǐ, I.F., Deev, S.M., Balandin, T.G., Leshchinskaia, I.B., Antiviral activity of Bacillus intermedius RNAase in guinea-pigs and rabbits infected with outdoor rabies virus (2006) Vopr. Virusol., 51, pp. 41-43Gribencha, S.V., Potselueva, L.A., Barinskiǐ, I.F., Balandin, T.G., Deev, S.M., Leshchinskaia, I.B., The antiviral activity of RNAse Bacillus intermedius in experiments with mice preinfected with street rabies virus (2004) Vopr. Virusol., 49, pp. 38-41Zhou, W.W., Niu, T.G., Purification and some properties of an extracellular ribonuclease with antiviral activity against tobacco mosaic virus from Bacillus cereus (2009) Biotechnol. Lett., 31, pp. 101-105Boya, C.A., Herrera, L., Guzman, H.M., Gutierrez, M., Antiplasmodial activity of bacilosarcinA isolated from the octocoral-associated bacterium Bacillus sp. collected in Panama (2012) J. Pharm. Bioallied. Sci., 4, pp. 66-69El-Sersy, N.A., Abdelwahab, A.E., Abouelkhiir, S.S., Antibacterial and anticancer activity of ε-poly-L-lysine (ε-PL) produced by a marine Bacillus subtilis sp (2012) J. Basic Microbiol., 52, pp. 513-522Craczyc, K., Trojanowska, K., Mueller, A., Antifungal activity of Bacillus coagulans against Fusarium sp (2002) Acta Microbiol. Pol., 51, pp. 275-283Sohn, M.J., Zheng, C.J., Kim, W.G., Macrolactin S, a new antibacterial agent with FabG-inhibitory activity from Bacillus sp. AT28 (2008) J. Antibiot., 61, pp. 687-691Moshafi, M.H., Forootanfar, H., Ameri, A., Antimicrobial activity of Bacillus sp. strain FAS1 isolated from soil. Pak (2011) J. Pharm. Sci., 24, pp. 269-27

Controle massal da broca‑do‑café com armadilhas de garrafa Pet vermelha em cafeeiro

The objective of this work was to evaluate the efficacy of red PET‑bottle traps, containing attractive alcoholic volatile compounds, in the mass control of coffee berry borer (Hypothenemus hampei). The evaluations were carried out in four coffee crops, during two years. Nine hundred PET‑bottle traps, painted red, were distributed in three of the four coffee crops; one crop without traps was used as a control. Holes drilled into berries (%) were determined in these crops. There was a reduction in drilled berries in crops with traps, from the 2007/2008 harvest to the 2008/2009 harvest. The highest coffee berry borer densities in traps was observed in the flowering and small‑green berries stages. The red PET‑bottle trap is efficient in the mass control of the coffee berry borer population, since it reduces the percentage of drilled berries in 57%; however, this reduction is not enough to keep borer densities below the control level.O objetivo deste trabalho foi avaliar a eficácia de armadilha de garrafa Pet vermelha contendo compostos voláteis alcoólicos atrativos, no controle massal da broca‑do‑café (Hypothenemus hampei). As avaliações foram realizadas em quatro lavouras de café, durante dois anos. Foram distribuídas 900 armadilhas de garrafa Pet, pintadas de vermelho, em três das quatro lavouras; uma lavoura sem armadilhas foi usada como controle. O broqueamento dos frutos (%) foi determinado nessas lavouras. Houve redução do broqueamento de frutos nas lavouras com armadilhas, da safra 2007/2008 para a safra 2008/2009. As maiores densidades da broca‑do‑café nas armadilhas foram observadas nos estádios de floração e de frutos chumbinho. A armadilha de garrafa Pet vermelha é eficaz no controle massal da população da broca‑do‑café, por reduzir a percentagem de frutos broqueados em 57%; entretanto, essa redução não é suficiente para manter as densidades da broca abaixo do nível de controle

Study of Tau-pair Production in Photon-Photon Collisions at LEP and Limits on the Anomalous Electromagnetic Moments of the Tau Lepton

Tau-pair production in the process e+e- -> e+e-tau+tau- was studied using data collected by the DELPHI experiment at LEP2 during the years 1997 - 2000. The corresponding integrated luminosity is 650 pb^{-1}. The values of the cross-section obtained are found to be in agreement with QED predictions. Limits on the anomalous magnetic and electric dipole moments of the tau lepton are deduced.Comment: 20 pages, 9 figures, Accepted by Eur. Phys. J.