86 research outputs found
Interstellar Dust Inside and Outside the Heliosphere
In the early 1990s, after its Jupiter flyby, the Ulysses spacecraft
identified interstellar dust in the solar system. Since then the in-situ dust
detector on board Ulysses continuously monitored interstellar grains with
masses up to 10e-13 kg, penetrating deep into the solar system. While Ulysses
measured the interstellar dust stream at high ecliptic latitudes between 3 and
5 AU, interstellar impactors were also measured with the in-situ dust detectors
on board Cassini, Galileo and Helios, covering a heliocentric distance range
between 0.3 and 3 AU in the ecliptic plane. The interstellar dust stream in the
inner solar system is altered by the solar radiation pressure force,
gravitational focussing and interaction of charged grains with the time varying
interplanetary magnetic field. The grains act as tracers of the physical
conditions in the local interstellar cloud (LIC). Our in-situ measurements
imply the existence of a population of 'big' interstellar grains (up to 10e-13
kg) and a gas-to-dust-mass ratio in the LIC which is a factor of > 2 larger
than the one derived from astronomical observations, indicating a concentration
of interstellar dust in the very local interstellar medium. Until 2004, the
interstellar dust flow direction measured by Ulysses was close to the mean apex
of the Sun's motion through the LIC, while in 2005, the data showed a 30 deg
shift, the reason of which is presently unknown. We review the results from
spacecraft-based in-situ interstellar dust measurements in the solar system and
their implications for the physical and chemical state of the LIC.Comment: 10 pages, 2 b/w figures, 1 colour figure; submitted to Space Science
Review
Galileo dust data from the jovian system: 2000 to 2003
The Galileo spacecraft was orbiting Jupiter between Dec 1995 and Sep 2003.
The Galileo dust detector monitored the jovian dust environment between about 2
and 370 R_J (jovian radius R_J = 71492 km). We present data from the Galileo
dust instrument for the period January 2000 to September 2003. We report on the
data of 5389 particles measured between 2000 and the end of the mission in
2003. The majority of the 21250 particles for which the full set of measured
impact parameters (impact time, impact direction, charge rise times, charge
amplitudes, etc.) was transmitted to Earth were tiny grains (about 10 nm in
radius), most of them originating from Jupiter's innermost Galilean moon Io.
Their impact rates frequently exceeded 10 min^-1. Surprisingly large impact
rates up to 100 min^-1 occurred in Aug/Sep 2000 when Galileo was at about 280
R_J from Jupiter. This peak in dust emission appears to coincide with strong
changes in the release of neutral gas from the Io torus. Strong variability in
the Io dust flux was measured on timescales of days to weeks, indicating large
variations in the dust release from Io or the Io torus or both on such short
timescales. Galileo has detected a large number of bigger micron-sized
particles mostly in the region between the Galilean moons. A surprisingly large
number of such bigger grains was measured in March 2003 within a 4-day interval
when Galileo was outside Jupiter's magnetosphere at approximately 350 R_J
jovicentric distance. Two passages of Jupiter's gossamer rings in 2002 and 2003
provided the first actual comparison of in-situ dust data from a planetary ring
with the results inferred from inverting optical images.Comment: 59 pages, 13 figures, 6 tables, submitted to Planetary and Space
Scienc
One year of Galileo dust data from the Jovian system: 1996
The dust detector system onboard Galileo records dust impacts in circumjovian
space since the spacecraft has been injected into a bound orbit about Jupiter
in December 1995. This is the sixth in a series of papers dedicated to
presenting Galileo and Ulysses dust data. We present data from the Galileo dust
instrument for the period January to December 1996 when the spacecraft
completed four orbits about Jupiter (G1, G2, C3 and E4). Data were obtained as
high resolution realtime science data or recorded data during a time period of
100 days, or via memory read-outs during the remaining times. Because the data
transmission rate of the spacecraft is very low, the complete data set (i. e.
all parameters measured by the instrument during impact of a dust particle) for
only 2% (5353) of all particles detected could be transmitted to Earth; the
other particles were only counted. Together with the data for 2883 particles
detected during Galileo's interplanetary cruise and published earlier, complete
data of 8236 particles detected by the Galileo dust instrument from 1989 to
1996 are now available. The majority of particles detected are tiny grains
(about 10 nm in radius) originating from Jupiter's innermost Galilean moon Io.
These grains have been detected throughout the Jovian system and the highest
impact rates exceeded . A small number of grains has been
detected in the close vicinity of the Galilean moons Europa, Ganymede and
Callisto which belong to impact-generated dust clouds formed by (mostly
submicrometer sized) ejecta from the surfaces of the moons (Kr\"uger et al.,
Nature, 399, 558, 1999). Impacts of submicrometer to micrometer sized grains
have been detected thoughout the Jovian system and especially in the region
between the Galilean moons.Comment: accepted for Planetary and Space Science, 33 pages, 6 tables, 10
figure
Four years of Ulysses dust data: 1996 to 1999
The Ulysses spacecraft is orbiting the Sun on a highly inclined ellipse (, perihelion distance 1.3 AU, aphelion distance 5.4 AU). Between
January 1996 and December 1999 the spacecraft was beyond 3 AU from the Sun and
crossed the ecliptic plane at aphelion in May 1998. In this four-year period
218 dust impacts were recorded with the dust detector on board. We publish and
analyse the complete data set of both raw and reduced data for particles with
masses to g. Together with 1477 dust impacts
recorded between launch of Ulysses and the end of 1995 published earlier
\cite{gruen1995c,krueger1999b}, a data set of 1695 dust impacts detected with
the Ulysses sensor between October 1990 and December 1999 is now available. The
impact rate measured between 1996 and 1999 was relatively constant with about
0.2 impacts per day. The impact direction of the majority of the impacts is
compatible with particles of interstellar origin, the rest are most likely
interplanetary particles. The observed impact rate is compared with a model for
the flux of interstellar dust particles. The flux of particles several
micrometers in size is compared with the measurements of the dust instruments
on board Pioneer 10 and Pioneer 11 beyond 3 AU (Humes 1980, JGR, 85,
5841--5852, 1980). Between 3 and 5 AU, Pioneer results predict that Ulysses
should have seen five times more ( sized) particles than
actually detected.Comment: accepted by Planetary and Space Science, 22 pages, 8 figures (1
colour figure
TrakEM2 Software for Neural Circuit Reconstruction
A key challenge in neuroscience is the expeditious reconstruction of neuronal circuits. For model systems such as Drosophila and C. elegans, the limiting step is no longer the acquisition of imagery but the extraction of the circuit from images. For this purpose, we designed a software application, TrakEM2, that addresses the systematic reconstruction of neuronal circuits from large electron microscopical and optical image volumes. We address the challenges of image volume composition from individual, deformed images; of the reconstruction of neuronal arbors and annotation of synapses with fast manual and semi-automatic methods; and the management of large collections of both images and annotations. The output is a neural circuit of 3d arbors and synapses, encoded in NeuroML and other formats, ready for analysis
Emergent Functional Properties of Neuronal Networks with Controlled Topology
The interplay between anatomical connectivity and dynamics in neural networks plays a key role in the functional properties of the brain and in the associated connectivity changes induced by neural diseases. However, a detailed experimental investigation of this interplay at both cellular and population scales in the living brain is limited by accessibility. Alternatively, to investigate the basic operational principles with morphological, electrophysiological and computational methods, the activity emerging from large in vitro networks of primary neurons organized with imposed topologies can be studied. Here, we validated the use of a new bio-printing approach, which effectively maintains the topology of hippocampal cultures in vitro and investigated, by patch-clamp and MEA electrophysiology, the emerging functional properties of these grid-confined networks. In spite of differences in the organization of physical connectivity, our bio-patterned grid networks retained the key properties of synaptic transmission, short-term plasticity and overall network activity with respect to random networks. Interestingly, the imposed grid topology resulted in a reinforcement of functional connections along orthogonal directions, shorter connectivity links and a greatly increased spiking probability in response to focal stimulation. These results clearly demonstrate that reliable functional studies can nowadays be performed on large neuronal networks in the presence of sustained changes in the physical network connectivity
Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping
To facilitate fine-scale phenotyping of whole specimens, we describe here a set of tissue fixation-embedding, detergent-clearing and staining protocols that can be used to transform excised organs and whole organisms into optically transparent samples within 1–2 weeks without compromising their cellular architecture or endogenous fluorescence. PACT (passive CLARITY technique) and PARS (perfusion-assisted agent release in situ) use tissue-hydrogel hybrids to stabilize tissue biomolecules during selective lipid extraction, resulting in enhanced clearing efficiency and sample integrity. Furthermore, the macromolecule permeability of PACT- and PARS-processed tissue hybrids supports the diffusion of immunolabels throughout intact tissue, whereas RIMS (refractive index matching solution) grants high-resolution imaging at depth by further reducing light scattering in cleared and uncleared samples alike. These methods are adaptable to difficult-to-image tissues, such as bone (PACT-deCAL), and to magnified single-cell visualization (ePACT). Together, these protocols and solutions enable phenotyping of subcellular components and tracing cellular connectivity in intact biological networks
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