Prevalence of Thermophilic Campylobacter species in carcasses from sheep and goats in an abattoir in Debre Zeit area, Ethiopia

Background: Thermophilic Campylobacter spp. namely, Campylobacter jejuni and coli cause acute diarrheal diseases in humans worldwide; although these species are known to occur in the intestinal tract of a wide variety of domestic and wild animals. Objective: Little is known about the presence of these bacteria in various food animals as possible sources of infection to humans in Ethiopia. Therefore this study was undertaken to determine the prevalence of thermophilic Campylobacter species in sheep and goat carcasses at a private export abattoir in Debre-Zeit, Ethiopia.Methods: A cross-sectional study was conducted on apparently healthy sheep and goat slaughtered at a private export abattoir in Debre-Zeit, from October 2007 to March 2008. Sheep carcasses (mutton) (n=218) and goat carcasses (n=180) were analyzed for Campylobacter spp. Swabs were taken from four different sites on the carcasses (crutch, abdomen, thorax and breast) at different stages of slaughtering processes (before evisceration, after evisceration and after washing). Results: Campylobacter spp were isolated from 40 (10.1%) out of 398 carcasses examined. There was no statistically significant difference in the rate of isolation of Campylobacter species in different swabbing sites. Of the 40 thermophilic campylobacter isolates, C. jejuni and C. coli accounted for 29 (72.5%) and 11 (27.5%), respectively. Conclusions: The results of this study revealed the presence of campylobacter in sheep and goat carcasses, indicatingpossible risks of infection to people through the consumption of raw/under-cooked meat. Coordinated actions are needed to reduce or eliminate the risks posed by this organism at various stages of slaughtering process

Feed resources availability, utilization and marketing in central and eastern Tigray, northern Ethiopia

This survey explored the situation of different feed resources for ruminant livestock in two zones (central and eastern) in Tigray region of northern Ethiopia. These two zones account for the region’s 12% of the total land mass, 23% of the human population, 28% of the cattle population, and 56% of the small ruminant population. Farmers feed their livestock a variety of feed resources that range from crop residues to non-conventional feeds.The overall contribution of crop residues exceeds 50% of the livestock feeds currently used by smallholder farmers, and this reflects the level of integration between crop and livestock farming. Overall, the management and utilization of crop residues with particular attention to collection and transportation is constrained by factors such as labour shortage, distance from harvesting field to animals and delayed harvest in the project districts. Grazing land management and improvement is seldom considered by farmers or the extension system. Acute shortages of AIBPs supply from the sources and high price fluctuation in the main towns of the districts are discouraging smallholder dairy and small ruminant farmers from using them on a regular basis. The contribution of improved forage plants is not as expected and forage genetic material multiplication sites are operating with insufficient budget and manpower, and not supported by proper research. The overall feed supply to demand ratio in four districts in the eastern zone is not enough to cover the annual maintenance requirements of the present stock

Calcium deficiency and causation of rickets in Ethiopian children

Objective: To assess the role of calcium in the development of clinical rickets among Ethiopian children coming to Jimma Specialised Hospital outpatient, department. Design: Case control study. Settings: Jimma Specialised Teaching Hospital and surrounding urban and rural community in the catchment area. Subjects: One hundred and thirty five under five year old children (30 cases of rickets and 104 non-rachitic cases) who came for paediatric service in Jimma hospital. Results: The mean (±SD) level of calcium intake for cases and controls was 664.5 (± 102.7) mg and 645.8(± 144.4) mg, respectively. There was no statistically significance difference between the two groups in the mean level of calcium intake (t=0.659, P>0.05). Generally, in both cases and controls the majority of the study participants were taking calcium below the recommended daily calcium requirement of 800 mg for age group according to Food and Nutrition Board of the National Research Council. When adjusted for different co-variates including age, sex, breast feeding history, history of diarrhoea lasting longer than 14 days and 24 hours calcium intake children and religion, occupation, educational status and residence the mothers/care givers using logistic regression model, frequency of exposure to sunlight ( OR=1.55, 95%CI: 1.2, 2.0), being from rural areas(OR= 5.0, 95% CI: 1.1, 23.3) and age 12-23 months (OR= 4.5, 95%CI: 1.2, 16.5) were significantly associated with rickets(

EP-1430: Phase II study of short-course accelerated palliative radiation therapy for advanced thoracic tumors

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An ethnobotanical study of medicinal plants used by local people in the lowlands of Konta Special Woreda, southern nations, nationalities and peoples regional state, Ethiopia

<p>Abstract</p> <p>Background</p> <p>Research was carried out in Konta Special Woreda (District); it is a remote area with lack of infrastructure like road to make any research activities in the area. Therefore, this research was conducted to investigate medicinal plants of the Konta people and to document the local knowledge before environmental and cultural changes deplete the resources.</p> <p>Methods</p> <p>The information was collected between October 2006 and February 2007. Interview-based field study constituted the main data collection method in which the gathering, preparation, use, previous and current status and cultivation practices were systematically investigated. The abundance, taxonomic diversity and distribution of medicinal plants were studied using ecological approach.</p> <p>Results</p> <p>A total of 120 species, grouped within 100 genera and 47 families that are used in traditional medical practices were identified and studied. The Fabaceae and Lamiaceae were the most commonly reported medicinal plants with 16 (13.3%) and 14 (12%) species, respectively. 25.4% of the total medicinal plants are collected from homegardens and the rest (74.6%) are collected from wild habitats. Of the total number of medicinal plants, 108 species (90%) were used to treat human ailments, 6 (5%) for livestock diseases and the remaining 6 (5%) were used to treat both human and livestock health problems. The major threats to medicinal plants reported include harvesting medicinal plants for firewood (24.8%) followed by fire (22.3%) and construction (19%). Of the four plant communities identified in the wild, more medicinal plant species (34) were found in community type-4 (<it>Hyparrhenia cymbaria</it>-<it>Erythrina abyssinica </it>community), which accounted for 61.8%.</p> <p>Conclusion</p> <p>Konta Special Woreda is an important area for medicinal plants and associated local knowledge; the natural vegetation being the most important reservoir for the majority of the medicinal plants. Environmental and cultural changes are in the process of threatening the resources and this signals the need for serious efforts to create public awareness so that measures are taken to conserve the medicinal plants in the natural ecosystems and other suitable environments.</p

Clonal Differences between Non-Typhoidal Salmonella (NTS) Recovered from Children and Animals Living in Close Contact in The Gambia

Salmonellosis is a neglected tropical disease causing serious dysentery and septicaemia particularly in young infants, elderly and immunocompromised individuals such as HIV patients and associated with substantial mortality in developing countries. Salmonellosis also constitutes a major public health problem as it is considered the most widespread bacterial zoonosis of food origin throughout the world. Many epidemiological data exist from developed countries concerning transmission of Non-Typhoidal Salmonella (NTS) but few are available from developing countries. In addition few studies in sub-Saharan Africa have considered the interface between humans and their environment in relation to animals present in the household and food hygiene. This study describes the prevalence of NTS among fourteen Gambian children and 210 domestic animals living in close proximity (household) to the children in a rural setting in The Gambia. We found that the domestic animals living in the same household as patients carried different NTS serovar and genotypes; indicating that zoonotic transmission does not occur in our setting. This study provides baseline data for future studies of transmission of NTS in rural Africa

Healthcare workers' perspectives and practices regarding the disclosure of HIV status to children in Malawi: A cross-sectional study

Background: In 2011 the World Health Organisation recommended that children with a diagnosis of HIV be gradually informed about their HIV status between the ages of 6 and 12 years. However, to date, literature has focused mainly on primary caregiver and child experiences with HIV disclosure, little is known about healthcare workers' perspectives and practices of HIV status disclosure to children. The aim of this study was to assess healthcare workers' perspectives and practices regarding the disclosure of HIV status to children aged between 6 and 12 years in Malawi. Methods: A cross-sectional survey was used to collect data from 168 healthcare providers working in antiretroviral clinics in all government District and Tertiary Hospitals in Malawi. Participants were asked questions regarding their knowledge, practice, and barriers to HIV disclosure. Data were analysed using binary logistic regression. Results: Almost all healthcare workers (98%) reported that it was important to disclose HIV status to children. A significant proportion (37%) reported that they had never disclosed HIV status to a child and about half estimated that the rate of HIV disclosure at their facility was 25% or less. The main barriers to disclosure were lack of training on disclosure (85%) and lack of a standard tool for disclosure (84%). Female healthcare workers (aOR) 2.4; 95% CI: 1.1-5.5) and lack of training on disclosure (aOR 7.7; 95% CI: 3.4-10.7) were independently associated with never having disclosed HIV status to a child. Conclusions: This study highlights the need for providing appropriate training in HIV disclosure for healthcare workers and the provision of standardised disclosure materials