Studies of an x ray selected sample of cataclysmic variables

Just prior to the thesis research, an all-sky survey in hard x rays with the HEAO-1 satellite and further observations in the optical resulted in a catalog of about 700 x-ray sources with known optical counterparts. This sample includes 43 cataclysmic variables, which are binaries consisting of a detached white-dwarf and a Roche lobe filling companion star. This thesis consists of studies of the x-ray selected sample of catalcysmic variables

Broken symmetries and pattern formation in two-frequency forced Faraday waves

We exploit the presence of approximate (broken) symmetries to obtain general scaling laws governing the process of pattern formation in weakly damped Faraday waves. Specifically, we consider a two-frequency forcing function and trace the effects of time translation, time reversal and Hamiltonian structure for three illustrative examples: hexagons, two-mode superlattices, and two-mode rhomboids. By means of explicit parameter symmetries, we show how the size of various three-wave resonant interactions depends on the frequency ratio m:n and on the relative temporal phase of the two driving terms. These symmetry-based predictions are verified for numerically calculated coefficients, and help explain the results of recent experiments.Comment: 4 pages, 6 figure

Simultaneous IUE, EXOSAT and optical observations of the unusual AM Her type variable H058+608

Simultaneous observations of the AM Her type variable H0538+608 made with IUE, EXOSAT, and a 1.3 m ground based telescope, and subsequent optical spectrophotometry at high and low resolution are discussed. The X-ray and optical data show clear evidence of a 3.30 + or - 0.03 hr period. Three SWP spectra were taken outside of eclipse and during overlapping phase intervals. The UV spectra contain strong emission lines characteristic of this class of objects and a flat continuum which appears to be deficient, given the brightness of source at optical and X-ray wavelengths. There is evidence for intensity variations in emission lines, particularly C IV. The X-ray light curves for H0538+608 reveal behavior which may be related to irregularities in its accretion flow

Forcing function control of Faraday wave instabilities in viscous shallow fluids

We investigate the relationship between the linear surface wave instabilities of a shallow viscous fluid layer and the shape of the periodic, parametric-forcing function (describing the vertical acceleration of the fluid container) that excites them. We find numerically that the envelope of the resonance tongues can only develop multiple minima when the forcing function has more than two local extrema per cycle. With this insight, we construct a multi-frequency forcing function that generates at onset a non-trivial harmonic instability which is distinct from a subharmonic response to any of its frequency components. We measure the corresponding surface patterns experimentally and verify that small changes in the forcing waveform cause a transition, through a bicritical point, from the predicted harmonic short-wavelength pattern to a much larger standard subharmonic pattern. Using a formulation valid in the lubrication regime (thin viscous fluid layer) and a WKB method to find its analytic solutions, we explore the origin of the observed relation between the forcing function shape and the resonance tongue structure. In particular, we show that for square and triangular forcing functions the envelope of these tongues has only one minimum, as in the usual sinusoidal case.Comment: 12 pages, 10 figure

JAK2V617F promotes replication fork stalling with disease-restricted impairment of the intra-S checkpoint response

Cancers result from the accumulation of genetic lesions, but the cellular consequences of driver mutations remain unclear, especially during the earliest stages of malignancy. The V617F mutation in the JAK2 non-receptor tyrosine kinase (JAK2V617F) is present as an early somatic event in most patients with myeloproliferative neoplasms (MPNs), and the study of these chronic myeloid malignancies provides an experimentally tractable approach to understanding early tumorigenesis. Introduction of exogenous JAK2V617F impairs replication fork progression and is associated with activation of the intra-S checkpoint, with both effects mediated by phosphatidylinositide 3-kinase (PI3K) signaling. Analysis of clonally derived JAK2V617F-positive erythroblasts from MPN patients also demonstrated impaired replication fork progression accompanied by increased levels of replication protein A (RPA)-containing foci. However, the associated intra-S checkpoint response was impaired in erythroblasts from polycythemia vera (PV) patients, but not in those from essential thrombocythemia (ET) patients. Moreover, inhibition of p53 in PV erythroblasts resulted in more gamma-H2Ax (γ-H2Ax)–marked double-stranded breaks compared with in like-treated ET erythroblasts, suggesting the defective intra-S checkpoint function seen in PV increases DNA damage in the context of attenuated p53 signaling. These results demonstrate oncogene-induced impairment of replication fork progression in primary cells from MPN patients, reveal unexpected disease-restricted differences in activation of the intra-S checkpoint, and have potential implications for the clonal evolution of malignancies

Reseña. Parada, Alejandro E. (2019). Lectura y contralectura en la Historia de la Lectura. Villa María: Eduvim, 120 pp.

With the fall of the great stories at the end of the eighties of the last century, it has opened the way to rethink an activity that seemed confined to the private sphere: reading practices. From the history of reading (HL), a field belonging to the new cultural history, there has been a vast amount of research in this regard, which has motivated some to reflect

Super-lattice, rhombus, square, and hexagonal standing waves in magnetically driven ferrofluid surface

Standing wave patterns that arise on the surface of ferrofluids by (single frequency) parametric forcing with an ac magnetic field are investigated experimentally. Depending on the frequency and amplitude of the forcing, the system exhibits various patterns including a superlattice and subharmonic rhombuses as well as conventional harmonic hexagons and subharmonic squares. The superlattice arises in a bicritical situation where harmonic and subharmonic modes collide. The rhombic pattern arises due to the non-monotonic dispersion relation of a ferrofluid

A Theater-Based Approach to Primary Prevention of Sexual Behavior for Early Adolescents

Early adolescence is a crucial period for preventing teen pregnancy and sexually transmitted infections. This study evaluated STAR LO, a theater-based intervention designed to affect antecedents of sexual activity among urban early adolescents (N = 1,143). Public elementary/middle schools received the intervention or served as a wait-listed comparison group in a quasi-experimental study. Students completed pretest and posttest questionnaires. Multivariate regression models were used to examine treatment effects. Comparison students showed significantly greater increases in sexual intentions and decreases in pro-abstinence attitudes and intended age of first sex than treatment group adolescents. Comparison girls showed significantly greater increases in desire to be a teen parent than STAR LO girls. Treatment group adolescents exhibited greater increases in sexual knowledge than comparison group adolescents, with stronger effects for boys than girls. The results suggest that this theater-based prevention program can help early adolescents develop knowledge, attitudes, and intentions that may prevent future sexual risk behavior

Large, Sparse Optimal Matching with Refined Covariate Balance in an Observational Study of the Health Outcomes Produced by New Surgeons

Every newly trained surgeon performs her first unsupervised operation. How do the health outcomes of her patients compare with the patients of experienced surgeons? Using data from 498 hospitals, we compare 1252 pairs comprised of a new surgeon and an experienced surgeon working at the same hospital. We introduce a new form of matching that matches patients of each new surgeon to patients of an otherwise similar experienced surgeon at the same hospital, perfectly balancing 176 surgical procedures and closely balancing a total of 2.9 million categories of patients; additionally, the individual patient pairs are as close as possible. A new goal for matching is introduced, called refined covariate balance, in which a sequence of nested, ever more refined, nominal covariates is balanced as closely as possible, emphasizing the first or coarsest covariate in that sequence. A new algorithm for matching is proposed and the main new results prove that the algorithm finds the closest match in terms of the total within-pair covariate distances among all matches that achieve refined covariate balance. Unlike previous approaches to forcing balance on covariates, the new algorithm creates multiple paths to a match in a network, where paths that introduce imbalances are penalized and hence avoided to the extent possible. The algorithm exploits a sparse network to quickly optimize a match that is about two orders of magnitude larger than is typical in statistical matching problems, thereby permitting much more extensive use of fine and near-fine balance constraints. The match was constructed in a few minutes using a network optimization algorithm implemented in R. An R package called rcbalance implementing the method is available from CRAN

Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion

Recent descriptions of significant heterogeneity in normal stem cells and cancers have altered our understanding of tumorigenesis, emphasizing the need to understand how single stem cells are subverted to cause tumors. Human myeloproliferative neoplasms (MPNs) are thought to reflect transformation of a hematopoietic stem cell (HSC) and the majority harbor an acquired V617F mutation in the JAK2 tyrosine kinase, making them a paradigm for studying the early stages of tumor establishment and progression. The consequences of activating tyrosine kinase mutations for stem and progenitor cell behavior are unclear. In this article, we identify a distinct cellular mechanism operative in stem cells. By using conditional knock-in mice, we show that the HSC defect resulting from expression of heterozygous human JAK2V617F is both quantitative (reduced HSC numbers) and qualitative (lineage biases and reduced self-renewal per HSC). The defect is intrinsic to individual HSCs and their progeny are skewed toward proliferation and differentiation as evidenced by single cell and transplantation assays. Aged JAK2V617F show a more pronounced defect as assessed by transplantation, but mice that transform reacquire competitive self-renewal ability. Quantitative analysis of HSC-derived clones was used to model the fate choices of normal and JAK2-mutant HSCs and indicates that JAK2V617F reduces self-renewal of individual HSCs but leaves progenitor expansion intact. This conclusion is supported by paired daughter cell analyses, which indicate that JAK2-mutant HSCs more often give rise to two differentiated daughter cells. Together these data suggest that acquisition of JAK2V617F alone is insufficient for clonal expansion and disease progression and causes eventual HSC exhaustion. Moreover, our results show that clonal expansion of progenitor cells provides a window in which collaborating mutations can accumulate to drive disease progression. Characterizing the mechanism(s) of JAK2V617F subclinical clonal expansions and the transition to overt MPNs will illuminate the earliest stages of tumor establishment and subclone competition, fundamentally shifting the way we treat and manage cancers