Odor Compound Detection in Male Euglossine Bees

Male euglossine bees collect fragrances from various sources, which they store and use for as yet unknown purposes. They are attracted, often specifically, to single odor compounds and blends thereof. We used gas chromatography with electroantennographic detection (GC-EAD) and electroantennography (EAG) to investigate the response to 8 odor compounds by males of two euglossine species, Euglossa cybelia Moure and Eulaema polychroma (Mocsàry). In E. cybelia, we recorded EAD reactions in response to 1,8-cineole, methyl benzoate, benzyl actetate, methyl salicylate, eugenbl, and methyl cinnamate. E. polychroma responded to the same compounds in EAG experiments, while (ls)(-)α-pinene and β-pinene failed to trigger EAD or EAG responses in the bees. Blends of two compounds triggered larger responses than single compounds in EAG experiments with E. polychroma, however, when α-pinene was added, reactions decreased. In the light of existing data on the bees' behavior towards these odor compounds, our work indicates that both peripheral and central nervous processes influence the attraction of euglossine bees to odor

Color-dependent learning in restrained africanized honey bees

Q2Q1Associative color learning has been demonstrated to be very poor using restrained European honey bees unless the antennae are amputated. Consequently, our understanding of proximate mechanisms in visual information processing is handicapped. Here we test learning performance of Africanized honey bees under restrained conditions with visual and olfactory stimulation using the proboscis extension response (PER) protocol. Restrained individuals were trained to learn an association between a color stimulus and a sugar–water reward. We evaluated performance for ‘absolute’ learning (learned association between a stimulus and a reward) and ‘discriminant’ learning (discrimination between two stimuli). Restrained Africanized honey bees (AHBs) readily learned the association of color stimulus for both blue and green LED stimuli in absolute and discriminatory learning tasks within seven presentations, but not with violet as the rewarded color. Additionally, 24-h memory improved considerably during the discrimination task, compared with absolute association (15–55%). We found that antennal amputation was unnecessary and reduced performance in AHBs. Thus color learning can now be studied using the PER protocol with intact AHBs. This finding opens the way towards investigating visual and multimodal learning with application of neural techniques commonly used in restrained honey bees.https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000002842Revista Nacional - Indexad

Description of an ancient social bee trapped in amber using diagnostic radioentomology

The application of non-invasive imaging technologies using X-radiation (diagnostic radioentomology, ‘DR') is demonstrated for the study of amber-entombed social bees. Here, we examine the external and internal morphology of an Early Miocene (Burdigalian) stingless bee (Apinae: Meliponini) from the Dominican Republic using non-destructive X-ray microtomography analysis. The study permits the accurate reconstruction of features otherwise obscured or impossible to visualize without destroying the sample and allows diagnosis of the specimen as a new species, Proplebeia adbita Greco and Enge

Ecosystem turnover in palaeoecological records: the sensitivity of pollen and phytolith proxies to detecting vegetation change in southwestern Amazonia

Identification of ecosystem turnover in the palaeo-vegetation record is important for understanding the resilience of ecosystems to past environmental change. There is uncertainty over the ability of different types of palaeo-vegetation proxy to detect ecosystem turnover. The aim of this paper is to compare the sensitivity of two palaeo-vegetation proxies - pollen and phytoliths - to changes within and between three key tropical South American ecosystems: evergreen forest, dry forest and savannah. A quantitative approach is used to assess the sensitivity of these two proxies to vegetation changes, based on the variability of proxy assemblages from 1-hectare ecological plots in ecotonal south west Amazonia. This modern dataset of proxy variability within evergreen forest, dry forest and savannah plots is then used to define thresholds for proxy variability which differentiate floristic changes within an ecosystem from ecosystem turnover. These thresholds are applied to two palaeo-vegetation records from NE Bolivia. Our results show that pollen is more sensitive than phytoliths to changes within evergreen forest, but phytoliths are more sensitive than pollen to changes within dry forest. Both proxies were equally sensitive to changes within savannas. These are important considerations for palaeoecologists selecting proxies for the study of ecosystem turnover in the palaeo-record. Application of the thresholds to the palaeo-record demonstrated the utility of this quantitative approach for assessing the magnitude of vegetation change in the palaeo-record. This quantitative approach is therefore a useful tool to improve the identification of ecosystem turnover in the palaeo-record

Areas of natural occurrence of melipona scutellaris Latreille, 1811(Hymenoptera: Apidae) in the state of Bahia, Brazil.

The bee Melipona scutellaris is considered the reared meliponine species with the largest distribution in the North and Northeast regions of Brazil, with records from the state of Rio Grande do Norte down to the state of Bahia. Considering the importance of this species in the generation of income for family agriculture and in the preservation of areas with natural vegetation, this study aimed at providing knowledge on the distribution of natural colonies of M. scutellaris in the state of Bahia. Literature information, interviews with stinglessbee beekeepers, and expeditions were conducted to confirm the natural occurrence of the species. A total of 102 municipalities showed records for M. scutellaris, whose occurrence was observed in areas ranging from sea level up to 1,200-meter height. The occurrence of this species in the state of Bahia is considered to be restricted to municipalities on the coastal area and the Chapada Diamantina with its rainforests. Geographic coordinates, elevation, climate and vegetation data were obtained, which allowed a map to be prepared for the area of occurrence in order to support conservation and management policies for the species

Description of an ancient social bee trapped in amber using diagnostic radioentomology

The application of non-invasive imaging technologies using X-radiation (diagnostic radioentomology, ‘DR’) is demonstrated for the study of amber-entombed social bees. Here, we examine the external and internal morphology of an Early Miocene (Burdigalian) stingless bee (Apinae: Meliponini) from the Dominican Republic using non-destructive X-ray microtomography analysis. The study permits the accurate reconstruction of features otherwise obscured or impossible to visualize without destroying the sample and allows diagnosis of the specimen as a new species, Proplebeia adbita Greco and Engel

Large-Range Movements of Neotropical Orchid Bees Observed via Radio Telemetry

Neotropical orchid bees (Euglossini) are often cited as classic examples of trapline-foragers with potentially extensive foraging ranges. If long-distance movements are habitual, rare plants in widely scattered locations may benefit from euglossine pollination services. Here we report the first successful use of micro radio telemetry to track the movement of an insect pollinator in a complex and forested environment. Our results indicate that individual male orchid bees (Exaerete frontalis) habitually use large rainforest areas (at least 42–115 ha) on a daily basis. Aerial telemetry located individuals up to 5 km away from their core areas, and bees were often stationary, for variable periods, between flights to successive localities. These data suggest a higher degree of site fidelity than what may be expected in a free living male bee, and has implications for our understanding of biological activity patterns and the evolution of forest pollinators

Methods for Intensifying Biogas Production from Waste: A Scientometric Review of Cavitation and Electrolysis Treatments

This article presents future trends in research using microbiological methods to intensify bioprocesses for biogas production. The pretreatment by combinations of physical and chemical methods, such as cavitation and electrolysis, is considered. The approach of the article involved reviewing the residual area on the intensification technologies of anaerobic digestion with current methods to improve the quality and quantity of biogas. The most valuable reported positive results of the pretreatment of biological rawmaterials in the cavitation process were reviewed and are presented here. A model of the effect of electrolysis on the species diversity of bacteria in anaerobic digestion was developed, and changes in the dominance of the ecological and trophic systems were revealed on the basis of previous studies. The stimulating effect on biogas yield, reduction in the stabilization period of the reactor, and inactivation of microorganisms at lower temperatures is associated with different pretreatment methods that intensify anaerobic digestion. More research is recommended to focus on the electrolysis treatment of different types of waste and their ratios with optimization of regime parameters, as well as in combination with other pretreatments to produce biomethane and biohydrogen in larger quantities and in better qualities