The special Sm core structure of the U7 snRNP: far-reaching significance of a small nuclear ribonucleoprotein

Abstract.: The polypeptide composition of the U7 small nuclear ribonucleoprotein (snRNP) involved in histone messenger RNA (mRNA) 3′ end formation has recently been elucidated. In contrast to spliceosomal snRNPs, which contain a ring-shaped assembly of seven so-called Sm proteins, in the U7 snRNP the Sm proteins D1 and D2 are replaced by U7-specific Sm-like proteins, Lsm10 and Lsm11. This polypeptide composition and the unusual structure of Lsm11, which plays a role in histone RNA processing, represent new themes in the biology of Sm/Lsm proteins. Moreover this structure has important consequences for snRNP assembly that is mediated by two complexes containing the PRMT5 methyltransferase and the SMN (survival of motor neurons) protein, respectively. Finally, the ability to alter this polypeptide composition by a small mutation in U7 snRNA forms the basis for using modified U7 snRNA derivatives to alter specific pre-mRNA splicing events, thereby opening up a new way for antisense gene therap

Role of semantic paradigms for optimization of language mapping in clinical FMRI studies.

BACKGROUND AND PURPOSE: The optimal paradigm choice for language mapping in clinical fMRI studies is challenging due to the variability in activation among different paradigms, the contribution to activation of cognitive processes other than language, and the difficulties in monitoring patient performance. In this study, we compared language localization and lateralization between 2 commonly used clinical language paradigms and 3 newly designed dual-choice semantic paradigms to define a streamlined and adequate language-mapping protocol. MATERIALS AND METHODS: Twelve healthy volunteers performed 5 language paradigms: Silent Word Generation, Sentence Completion, Visual Antonym Pair, Auditory Antonym Pair, and Noun-Verb Association. Group analysis was performed to assess statistically significant differences in fMRI percentage signal change and lateralization index among these paradigms in 5 ROIs: inferior frontal gyrus, superior frontal gyrus, middle frontal gyrus for expressive language activation, middle temporal gyrus, and superior temporal gyrus for receptive language activation. RESULTS: In the expressive ROIs, Silent Word Generation was the most robust and best lateralizing paradigm (greater percentage signal change and lateralization index than semantic paradigms at P P P P CONCLUSIONS: The combination of Silent Word Generation and ≥1 visual semantic paradigm, such as Sentence Completion and Noun-Verb Association, is adequate to determine language localization and lateralization; Noun-Verb Association has the additional advantage of objective monitoring of patient performance

Ferromagnetic Ga₁ˍₓ Mnₓ As produced by ion implantation and pulsed-laser melting

We demonstrate the formation of ferromagneticGa₁ˍₓMnₓAsfilms by Mn ion implantation into GaAs followed by pulsed-laser melting. Irradiation with a single excimer laser pulse results in the epitaxial regrowth of the implanted layer with Mn substitutional fraction up to 80% and effective Curie temperature up to 29 K for samples with a maximum Mn concentration of x≈0.03. A remanent magnetization persisting above 85 K has been observed for samples with x≈0.10, in which 40% of the Mn resides on substitutional lattice sites. We find that the ferromagnetism in Ga₁ˍₓMnₓAs is rather robust to the presence of structural defects.The work at the Lawrence Berkeley National Laboratory was supported by the Director, Office of Science, Office of Basic Energy Sciences, Division of Materials Sciences and Engineering, of the U.S. Department of Energy under Contract No. DE-AC03-76SF00098. The work at Harvard was supported by NASA Grant No. NAG8-1680. One of the authors ~M.A.S.! acknowledges support from an NSF Graduate Research Fellowship

Lung Cancer and Human Papilloma Viruses (HPVs): Examining the Molecular Evidence

Human papilloma virus (HPV), known to be an etiological agent for genital cancers, has been suggested also to be a possible contributory agent for lung cancer. Alternatively, lung cancer, formerly considered to be solely a smoker's disease, may now be more appropriately categorised into never smoker's and smoker's lung cancer. Through this paper we attempt to bring forth the current knowledge regarding mechanisms of HPV gaining access into the lung tissue, various strategies involved in HPV-associated tumorigenesis in lung tissue

Aerosol optical depth studies during INDOEX: comparison of the spectral features over coastal India with the pristine southern hemispheric environment over Mauritius

Aerosol spectral optical depths, estimated using a ground-based network of multi wavelength radiometers (MWR) along the west coast of India [Trivandrum (TVM; 8.5°N, 77°E), and Minicoy (MCY; 8.3°N, 73.04°E)] and the pristine southern hemispheric environment at Mauritius (MRU; 20.26°S, 57.54°E) during the period January to June 1998 along with those obtained over the Arabian Sea and Indian Ocean during the INDOEX FFP-98 cruise (SK133) of ORV Sagar Kanya, are used to study the inter-hemispheric features of aerosols. Results indicate that there is a significant hemispherical difference for aerosol spectral optical depth (AOD) at shorter wavelengths (λ≤ 650 nm), while at the longer wavelengths (λ > 650 nm), AOD does not show any appreciable variation with location. The spectral variation of AOD at TVM and MCY (for March 1998) depicted a similar pattern with the AOD values between 0.5 and 0.6 at shorter wavelengths and between 0.2 and 0.4 at longer wavelengths. In contrast to this, the AOD at MRU are very low, lying in the range 0.1 to 0.2 in the shorter wavelengths, whereas at the longer wavelengths the AOD values are more or less comparable (in the range 0.2 to 0.4) with the northern hemispheric stations. The cruise data clearly showed that the transition occurs generally across the ITCZ. The increased AOD at shorter wavelengths in the northern hemisphere indicates higher concentration of sub-micron aerosols in these environments arising mainly due to anthropogenic activities, while the AOD at the longer wavelengths is attributed mainly to be of marine origin. In the post-cruise period, the spectral optical depths showed a gradual increase from March to June at MRU, while at TVM, the pattern followed more or less the climatological mean. By May the AOD at shorter wavelengths decreased at TVM (due to increased rainfall) and by June, the AOD at TVM are very much comparable with those seen at MRU, indicating a dominating marine aerosol influence at both these locations. The implications are discussed

A timeline for massive star-forming regions via combined observation of o-H2_2D+^+ and N2_2D+^+

Context: In cold and dense gas prior to the formation of young stellar objects, heavy molecular species (including CO) are accreted onto dust grains. Under these conditions H$_3^+$ and its deuterated isotopologues become more abundant, enhancing the deuterium fraction of molecules such as N$_2$H$^+$ that are formed via ion-neutral reactions. Because this process is extremely temperature sensitive, the abundance of these species is likely linked to the evolutionary stage of the source. Aims: We investigate how the abundances of o-H$_2$D$^+$ and N$_2$D$^+$ vary with evolution in high-mass clumps. Methods: We observed with APEX the ground-state transitions of o-H$_2$D$^+$ near 372 GHz, and N$_2$D$^+$(3-2) near 231 GHz for three massive clumps in different evolutionary stages. The sources were selected within the G351.77-0.51 complex to minimise the variation of initial chemical conditions, and to remove distance effects. We modelled their dust continuum emission to estimate their physical properties, and also modelled their spectra under the assumption of local thermodynamic equilibrium to calculate beam-averaged abundances. Results: We find an anticorrelation between the abundance of o-H$_2$D$^+$ and that of N$_2$D$^+$, with the former decreasing and the latter increasing with evolution. With the new observations we are also able to provide a qualitative upper limit to the age of the youngest clump of about 10$^5$ yr, comparable to its current free-fall time. Conclusions: We can explain the evolution of the two tracers with simple considerations on the chemical formation paths, depletion of heavy elements, and evaporation from the grains. We therefore propose that the joint observation and the relative abundance of o-H$_2$D$^+$ and N$_2$D$^+$ can act as an efficient tracer of the evolutionary stages of the star-formation process

The occurrence of live bait fish in south Andaman waters and its significance

It is well known that the key factor required for the initiation of a pole and line tuna fishery is the availability and abundance of suitable live bait fishes. While surveying the infestation of the crown of thorn starfish Acanthaster planci in the National Marine Park, Wandoor and adjacent areas of South Andamans during April - May, 1989 some preliminary observations on the availability of live bait fish in the area were recorded