Price and Profit: Investigating a Conundrum

Although few in number, studies consistently find that price explains little, if any, of the variation in profit across farms. This contrasts with farmers' opinions regarding the importance of price, as well as the use of price supports as a primary policy instrument. Using farm level data from the Illinois Farm Business Farm Management program for calendar years 1996 through 2004, a potential explanation for this conundrum is identified. Price is significantly more correlated with a farmer's variation in management return from year to year (approximately, +0.45) than with the variation in management return across farmers (approximately, +0.10). Thus, the conundrum arises out of different perspectives: farmers focus on the performance of their own farm over time while studies have focused on the variation among farms.Agricultural Finance,

Inhibitory neuron migration and IPL formation in the developing zebrafish retina.

The mature vertebrate retina is a highly ordered neuronal network of cell bodies and synaptic neuropils arranged in distinct layers. Little, however, is known about the emergence of this spatial arrangement. Here, we investigate how the three main types of retinal inhibitory neuron (RIN)--horizontal cells (HCs), inner nuclear layer amacrine cells (iACs) and displaced amacrine cells (dACs)--reach their specific laminar positions during development. Using in vivo time-lapse imaging of zebrafish retinas, we show that RINs undergo distinct phases of migration. The first phase, common to all RINs, is bipolar migration directed towards the apicobasal centre of the retina. All RINs then transition to a less directionally persistent multipolar phase of migration. Finally, HCs, iACs and dACs each undergo cell type-specific migration. In contrast to current hypotheses, we find that most dACs send processes into the forming inner plexiform layer (IPL) before migrating through it and inverting their polarity. By imaging and quantifying the dynamics of HCs, iACs and dACs from birth to final position, this study thus provides evidence for distinct and new migration patterns during retinal lamination and insights into the initiation of IPL formation.This work was supported by Wellcome Trust Senior Investigator Award 100329/Z/12/Z to WH and a UK Commonwealth Scholarship to RC.This is the final version of the article. It first appeared from the Company of Biologists via http://dx.doi.org/10.1242/dev.12247

A new bond fluctuation method for a polymer undergoing gel electrophoresis

We present a new computational methodology for the investigation of gel electrophoresis of polyelectrolytes. We have developed the method initially to incorporate sliding motion of tight parts of a polymer pulled by an electric field into the bond fluctuation method (BFM). Such motion due to tensile force over distances much larger than the persistent length is realized by non-local movement of a slack monomer at an either end of the tight part. The latter movement is introduced stochastically. This new BFM overcomes the well-known difficulty in the conventional BFM that polymers are trapped by gel fibers in relatively large fields. At the same time it also reproduces properly equilibrium properties of a polymer in a vanishing filed limit. The new BFM thus turns out an efficient computational method to study gel electrophoresis in a wide range of the electric field strength.Comment: 15 pages, 11 figure

Normal frames and the validity of the equivalence principle. I. Cases in a neighborhood and at a point

A treatment in a neighborhood and at a point of the equivalence principle on the basis of derivations of the tensor algebra over a manifold is given. Necessary and sufficient conditions are given for the existence of local bases, called normal frames, in which the components of derivations vanish in a neighborhood or at a point. These frames (bases), if any, are explicitly described and the problem of their holonomicity is considered. In particular, the obtained results concern symmetric as well as nonsymmetric linear connections.Comment: LaTeX2e, 9 pages, to be published in Journal of Physics A: Mathematical and Genera

Including historical data in the analysis of clinical trials

Data of previous trials with a similar setting are often available in the analysis of clinical trials. Several Bayesian methods have been proposed for including historical data as prior information in the analysis of the current trial, such as the (modified) power prior, the (robust) meta-analytic-predictive prior, the commensurate prior and methods proposed by Pocock and Murray et al. We compared these methods and illustrated their use in a practical setting, including an assessment of the comparability of the current and the historical data. The motivating data set consists of randomised controlled trials for acute myeloid leukaemia. A simulation study was used to compare the methods in terms of bias, precision, power and type I error rate. Methods that estimate parameters for the between-trial heterogeneity generally offer the best trade-off of power, precision and type I error, with the meta-analytic-predictive prior being the most promising method. The results show that it can be feasible to include historical data in the analysis of clinical trials, if an appropriate method is used to estimate the heterogeneity between trials, and the historical data satisfy criteria for comparability

Flows and particles with shear-free and expansion-free velocities in (L^-_n,g)- and Weyl's spaces

Conditions for the existence of flows with non-null shear-free and expansion-free velocities in spaces with affine connections and metrics are found. On their basis, generalized Weyl's spaces with shear-free and expansion-free conformal Killing vectors as velocity's vectors of spinless test particles moving in a Weyl's space are considered. The necessary and sufficient conditions are found under which a free spinless test particle could move in spaces with affine connections and metrics on a curve described by means of an auto-parallel equation. In Weyl's spaces with Weyl's covector, constructed by the use of a dilaton field, the dilaton field appears as a scaling factor for the rest mass density of the test particle. PACS numbers: 02.40.Ky, 04.20.Cv, 04.50.+h, 04.90.+eComment: 20 pages, LaTeX, to appear in Classical and Quantum Gravity. arXiv admin note: substantial text overlap with arXiv:gr-qc/001104

Increased Mobilisation of Circulating Endothelial Progenitors in von Hippel-Lindau Disease and Renal Cell Carcinoma

Background: Circulating endothelial cells (CECs) are a candidate biomarker for monitoring angiogenesis in cancer. Circulating endothelial cell subsets are mobilised by angiogenic mediators. Because of the highly angiogenic phenotype of renal cell carcinoma (RCC), we sought to assess the potential of CECs as a marker of RCC in patients with von Hippel-Lindau (VHL) disease and those with sporadic RCC. Methods: We performed multicolour flow cytometry to enumerate CECs in patients with RCC, patients with VHL disease with and without RCC, and normal subjects. Two subsets of CECs were evaluated: mature CECs (mCECs) and circulating endothelial progenitors (CEPs). Results: In patients with VHL disease and RCC and those with sporadic RCC (N=10), CEPs and the CEP:mCEC ratio were higher than in normal subjects (N=17) (median CEPs: 0.97 vs 0.19 cells $\mu l^{-1}$, respectively, P<0.01; median CEP:mCEC: 0.92 vs 0.58, respectively, P=0.04). However, in patients with VHL without RCC, CECs were not increased. In paired pre- and post-nephrectomy RCC patient samples (N=20), CEPs decreased after surgery (median difference 0.02 cells $\mu l^{-1}$, −0.06 to 1.2; P=0.05). Conclusion: Circulating endothelial progenitors were elevated in RCC, but not in patients with VHL without RCC. Circulating endothelial progenitor enumeration merits further investigation as a monitoring strategy for patients with VHL

Frames of reference in spaces with affine connections and metrics

A generalized definition of a frame of reference in spaces with affine connections and metrics is proposed based on the set of the following differential-geometric objects: (a) a non-null (non-isotropic) vector field, (b) the orthogonal to the vector field sub space, (c) an affine connection and the related to it covariant differential operator determining a transport along the given non-null vector filed. On the grounds of this definition other definitions related to the notions of accelerated, inertial, proper accelerated and proper inertial frames of reference are introduced and applied to some mathematical models for the space-time. The auto-parallel equation is obtained as an Euler-Lagrange's equation. Einstein's theory of gravitation appears as a theory for determination of a special frame of reference (with the gravitational force as inertial force) by means of the metrics and the characteristics of a material distribution. PACS numbers: 0490, 0450, 1210G, 0240VComment: 17 pages, LaTeX 2

Tricarbonyl M(I) (M = Re, 99mTc) complexes bearing acridine fluorophores : synthesis, characterization, DNA interaction studies and nuclear targeting

© The Royal Society of Chemistry 2010New pyrazolyl-diamine ligands with acridine derivatives at the 4-position of the pyrazolyl ring were synthesized and characterized (L1 and L2). Coordination towards the fac-[M(CO)3]+ (M = Re, 99mTc) led to complexes fac-[M(CO)3(κ3-L)] (L = L1: M = Re1, Tc1; L = L2: M = Re2, Tc2). The interaction of the novel pyrazolyl-diamine ligands (L1 and L2) and rhenium(I) complexes (Re1 and Re2) with calf thymus DNA (CT-DNA) was investigated by a variety of techniques, namely UV-visible , fluorescence spectroscopy and circular and linear dichroism . Compounds L1 and Re1 have moderate affinity to CT-DNA and bind to DNA by intercalation, while L2 and Re2 have a poor affinity for CT-DNA. Moreover, LD measurements showed that L1 and Re1 act as perfect intercalators . By confocal fluorescence microscopy we found that L1 and Re1 internalize and localize in the nucleus of B16F1 murine melanoma cells . The congener Tc1 complex also targets the cell nucleus exhibiting a time-dependent cellular uptake and a fast and high nuclear internalization (67.2% of activity after 30 min). Plasmid DNA studies have shown that Tc1 converts supercoiled (sc) puc19 DNA to the open circular (oc) form.Teresa Esteves and Sofia Gama thank the FCT for a doctoral and postdoctoral research grants (SFRH/BD/29154/2006 and SFRH/BPD/29564/2006, respectively). COST Action D39 is also acknowledge. The QITMS instrument was acquired with the support of the Programa Nacional de Reequipamento Científico (Contract>REDE/1503/REM/2005-ITN) of Fundação para a Ciência e a Tecnologia and is part of RNEM - Rede Nacional de Espectrometria de Massa