Nutrient Content of Napier Grass (Pennisetum Purpureum) Silage Made with Various Additive and Modified Atmosphere in the Silo

. During ensilage, anaerob condition must be controlled. Some methods of modified atmosphere in silo were analyzed to compare ensilage characteristics and silage product. So far, there is not been information on the atmosphere condition in the process of silage production. It encourages the researchers to evaluate the condition of ensilage process of Pennisetum purpureum by studying atmosphere modification in the silo and the effect of the USAge of various additives in the process of silage production. Elephant grass (Pennisetum purpureum), molasses, L. acidophillus were used. The study was conducted with a Completely Randomized Design (CRD) 3x2 factorial pattern. Atmosphere modification as the first factor consist of : (A0: silage with compaction (conventional) A1: silage with vacuum method, A2: silage with modified C02) and two kinds of silage additives as the second factor (B1: indirect additive (molasses); B2: direct additive (Lactic Acid Bacteria). Each treatment combination was repeated 4 times. The objective of the research was to evaluate changes in nutrient content (protein, crude fiber, gross energy). The data obtained were analyzed by analysis of variance, then continued by Honest Significant Differences (HSD) test. Based on the research results it can be concluded that the optimum ensilage can take place, either by compaction methods (conventional), vacuum and the addition of CO2. While the addition of molasses additive produces silage with better quality than the addition of L. Acidophillus inoculant

The Administration of Garlic Extract on Eimeria stiedai Oocysts and the Hematological Profile of the Coccidia Infected Rabbits

This research aimed to examine the potential of garlic as the coccidiosis control in rabbits either in vitro or in vivo. During in vitro, observed variables were rabbits oocysts that were sporulated, unsporulated, and abnormal in incubation for 3 days with the addition of garlic extract. The treatments were doses of garlic extract administration (0%, 1%, 2%, 4%, and 8%) and sulfaquinoxalline as a standard anticoccidiosis. Meanwhile during in vivo, the variables observed were the hematological profile of the experimental rabbits naturally infected with coccidia. The doses of garlic extract was administered orally to the experimental rabbits infected with coccidia were 0 mg, 10 mg, 20 mg, 40 mg, and 80 mg/rabbit.  As a standard coccidiosis drugs, the combination of sulfadiazine and trimethoprim was used.  The treatments were given for 6 days. The variables observed were the hematological profile of the coccidiosis rabbits, including the erythrocytes, hemoglobin, hematocrits, MCV, MCH, MCHC, and thrombocytes. The research employed a completely randomized design, with 5 repetitions. The data were further analyzed using the honestly significant difference test. The results showed that garlic extract administration significantly decreased (P<0.01) both the number of the sporulated and unsporulated oocysts (P<0.05), yet did not significantly influence the abnormal oocysts, but there was no significant difference within the entire hematological variables except in thrombocytes (P<0.05). Garlic extract administration decreased the excretion number of oocysts in the feces either in vitro or in vivo and influenced some hematological variables which provided a new propect for controlling coccidiosis naturally in rabbits

The Efficacies of Banana Stem Extract as a Candidate of Coccidiostat Against Rabbit Eimeria Stiedaio Ocysts: an in Vitro Analysis

The objective of this research was to investigatethe ability of banana stem (Musa paradisiaca) to inhibitsporulation of Eimeria stiedaioocystsderived fromrabbit by in vitroanalysis.Analyze the active substance proximate analysis and active substancesin this research were performed too. Banana stem extract were used in this experiment andsulfaquinoxalline(Coxy ®)was run as acontrol. The Eimeria stiedaioocystswere incubated prior the presence of different concentration from banana stem extract 0%, 1%, 2%, 4%, 8%for 1, 2 and 3 daysat 26°C. In addition,Factorial patterned Completely Randomized Design (CRD) with five replicates wasapplied on the experiment. Result analysis was performed by using Analysis of Variance and following by Honestly Significant Difference (HSD) post hoc test. Here, we identified that banana stem extract contain different type of active substance such as tannin, saponin, and alkaloid. Banana stem extract significantly affected the oocysts sporulation included the amount of sporulatedoocysts (P<0.01), unsporulatedoocysts (P<0.01), and transformed oocysts (P<0.01). In conclusion banana stem could inhibit the development of Eimeria stiedaioocysts on in vitroexperiment. HSD test showed that the optimum potential efficacy of banana stem toinhibit sporulation was at 4% and 8% concentration during three days incubation

Body Weight, Oocyte Elimination and Blood Profile of Rabbit After Challenge Test Using Eimeria Stiedai

The objective of the research was to investigate body weight, oocyte elimination and blood profile of rabbits infected with various doses of Eimeria stiedai isolates. The observed rabbits' blood profile included erythrocyte, hemoglobin, hematocrit, leucocyte, thrombocyte, total protein plasma (TPP) and fibrinogen. Twenty-five male New Zealand White rabbits aged 3 months and weighed approximately 2 kg were provided with pellet and boiled drinking water and Eimeria stiedai isolates. The experiment used Completely Randomized Design to analyze 5 treatments with five replicates. The examined variables included D0: Infection 0 (control of infection without challenge test), D1: Infection 101 with challenge test 103, D2: infection 102 with challenge test 103, D3: infection 103 with challenge test 103, D4: infection 0 with challenge test 103 (control of infection). Data were subject to analysis of variance followed by Honestly Significant Difference Test (HSD). Analysis of Variance result showed that there was no significant difference on body weight, oocyte elimination and blood profile including erythrocyte, hemoglobin, hematocrit, leucocyte, thrombocyte, and fibrinogen. However, total protein plasma (TTP) was significantly different at 5% HSD. It can be concluded that challenge test with Eimeria stiedai has not been used as an alternative in increasing rabbits' body immune against coccidiosis infection

Egg Production, Egg Quality, and Fatty Acid Profile of Indonesian Local Ducks Fed with Turmeric, Curcuma, and Probiotic Supplementation

Indonesian local ducks are commonly raised for egg production purposes. However, the performances of these ducks are still variable and must be improved. This study investigated the effects of turmeric, curcuma, and probiotic supplementations on the egg production and quality of Indonesian local ducks, emphasizing the eggs’ fatty acid profile. Two hundred female local ducks aged 16 weeks were randomly allotted to four dietary treatments with five replicates of 10 birds. The ducks were fed a corn and rice bran-based diet containing different supplements, i.e., a diet without supplementation as the control diet, a diet supplemented with turmeric at the level of 4%, a diet supplemented with curcuma at the level of 4%, and a diet supplemented with starbio probiotics at the level of 2%. The measured data were analyzed using analysis of variance using the 13 Systat program and continued with Duncan’s Multiple Range Test. Turmeric supplementation increased egg production compared with the control, and the duck fed probiotics consumed more feed than the control. Curcuma supplementation generated the lowest feed consumption, egg production, and physical egg quality than the other treatments (p&lt;0.05). The probiotics supplementation enhanced the blood high-density lipoprotein concentration (p&lt;0.05). Turmeric, curcuma, and probiotics supplementations generate variable responses in egg production and egg quality, including the fatty acid profile in the eggs. Turmeric and probiotics supplementations positively impact egg production, egg quality, or unsaturated fatty acid profile in the egg. However, curcuma supplementation decreased egg production and egg quality of local ducks. Furthermore, the fatty acid profile was not influenced by these supplements. It is concluded that supplementation of turmeric at the level of 4% and probiotics at the level of 2% in the diet can increase egg production and egg quality of local duck

Expression and Isolation of Recombinant Microneme 3 (MIC3) Protein of Toxoplasma gondii Local Isolate on Eschericia coli (BL21)

Abstract. Toxoplasmosis is a disease that infects all warm-blooded animals, including livestocks and humans caused by Toxoplasma gondii parasites. There are major drugs used for the therapy, though they have some effects to the patients, such as allergy, toxic and teratogenic for fetus. In addition, toxoplasmosis treatment is only effective for tachyzoites T. gondii in acute infection, while tissue cysts cannot be eradicated in chronic toxoplasmosis Tissue cysts of T. gondii contained in meat that are consumed by humans and meat-derived products may be important sources of infection for humans. Microneme protein (MIC) is one of proteins that belongs to excretory-secretory antigens (ESAs) of Toxoplasma gondii. Microneme 3 protein (MIC3) is the protein that plays an important role in the invasion process during cell infection as a mediator attachment parasite to the host cell. Recombinant MIC3 protein has been already used for the detection of toxoplasmosis and it could induce humoral and cellular immune response in experimental animals. The aim of this research was to express MIC3 recombinant protein of T. gondii from local isolate that was cloned into expression vector and transformed to E. coli BL21. In the future, recombinant protein MIC3 can be used for vaccine candidate and diagnostic tools for toxoplasmosis in animals and humans. Gene of MIC3 T. gondii local isolate (1.2 Kbp) was cloned into expression vector pET-32a(+) (5.9 Kbp) and transformed to Escherichia coli BL21. Protein from plasmid recombinant (7.1 Kbp) was expressed and performed by culturing recombinant bacteria into LB medium containing ampicillin and IPTG. Recombinant protein was isolated by sonication method and identified using SDS-PAGE. Finally, the recombinant protein was analyzed by immunoblotting using anti-ESAs polyclonal antibody. In conclusion, expression of the MIC3 gene with ~108 kDa has been successfully performed by cloning gene encoding for MIC3 protein of T. gondii local isolate that can be identified with polyclonal antibody anti-ESAs. Key Words: Toxoplasma gondii, expression, MIC3 protei