Influence of Different Vegetable Oils on In Vitro Ruminal Fermentability and Nutrient Digestibility in Ettawah Crossbred Goat

This research was aimed to determine the effect of supplementation of vegetable oil (corn oil, palm oil and kapok seed oil) on in vitro ruminal fermentability and nutrient digestibility. Experiment design used was Randomized Block Design with four treatments and four replication based on rumen fluid sampling time. The four treatments namely R0 = feed without oil supplementation; R1 = R0 + 5% corn oil; R2 = R0 + 5% palm oil; and R3 = R0 + 5% kapok seed oil. The result showed that supplementation of 5% vegetable oil did not affect (P>0,05) the rumen pH, A/P ratio, and efficiency of energy conversion. The total VFA, acetate, propionate, butyrate, methane and NH3 was higher (P<0,05) in supplementation of 5% vegetable oil than he control. The population of protozoa, microbial protein, in vitro dry matter digestibility (IVDMD), organic matter digestibility (IVOMD), and crude fiber digestibility (IVCFD) was lower (P<0,05) in supplementation of 5% compared to the control. Population of protozoa decreased until 58,76% for R1; 66,89% for R2; and 43,33% for R3. It can be concluded that supplementation of 5% vegetable oil decreased the population of protozoa, increased the production of VFA and NH3. Supplementation of 5% kapok seed oil resulting the highest of total VFA, acetate, propionate, butyrate and NH3 among other treatments

Polymorphism of Myostatin Gene (MSTN) Coding Region in Batur Sheep

The aim of present study was to investigate myostatin gene polymorphism and its association with weaning weight and body weight in Batur sheep, 30 heads of Batur all lambs were fed by concentrated feed. Body weight measured monthly after weaning to the six months of age. DNA Extraction used 200 ul of whole blood samples. To amplify exon 3 region of MSTN gene a specific primer designed using the Primer3 software. The 25 μl volume contained 25 ng of genomic DNA, 12.5 μl 2x Reaction mix of each primer. The cycling protocol was 5 minutes at 95°C as initial denaturation, 35 cycles of denaturing at 94°C for 45 seconds, annealing at 73.9 for 45 seconds, extending at 72°C for 40 s, with final extension at 72°C for 10 minutes. Eleven polymorphic sites were observed in the in 3rd exon region transversions at c.*121 G instead of A, del-T at c.*129, one individual at c.*139 and one individual at c.*158 positions however, one individual sequence disrupted reading frame in whole MSTN sequenced, also weaning weight and 6-month weight of Batur lambs were 21.13±5.70 and 31.64±7.06 to the homozygous whilst 19.99±4.33 and 30.50±5.44 for heterozygote respectively but there is no significe differences seen above all the studied parameters. Homozygous AA lambs had fairly low weaning weight and body weight compared to the heterozygous AB lambs but might have a higher carcass weight, further investigation is needed into the interaction of MSTN with other genes involved in muscle growth

The Internal Organs Weight of 6-Weeks Old Native Chickens after Supplement Addition With L-threonine and L-tryptophan in the Feed

The aim of this experiment was to identify the internal organs weight of 6-weeks old native chickens after treatment feed with l-threonine and l-tryptophan supplement. This experimental research used 128 native chickens aged one-week-old in a Completely Randomized Design with four treatments and four replications. The dietary treatments were T0 (control feed), T1 (T0 + 0.35% l-threonine + 0.10% l-tryptophan), T2 (T0 + 0.68% l-threonine + 0.17% l-tryptophan), and T3 (T0 + 1.00% l-threonine + 0.25% l-tryptophan). The variables measured included body weight, liver weight, pancreas weight, gizzard weight, and intestinal length. Statistical analysis showed that l-threonine and l-tryptophan significantly affected body weight, liver weight, pancreas weight, gizzard weight and intestinal length. Supplementing 1.00% l-threonine and 0.25% l- tryptophan to feed contributed to the highest body weight and internal organs weight of native chickens

The Effects of The Oxytocin Hormone Induction on The Value of The Composition of Etawah Crossbred Goat Milk

This study aims to see the extent of influence of oxytocin hormone induction on the nutritional composition of milk in the Etawah Crossbred  goat. This research was conducted in December 2018 in Syiah Kuala District, Banda Aceh, Indonesia. The examined parameters were the levels of fat, nonfat dry matter (BKTL), protein content, lactose content and milk density. The research used 18 Etawah goats and oxytocin hormone. A statistical unpaired t test indicated a highly significant influence (P 0.05) in milk, i.e. 8.39%, 3.91% and 3.76%, respectively. In conclusion, hormone oxytocin injection had a highly significant effect on increasing milk fat levels and significantly affected the decrease in milk density but did not affect the level of nonfat dry matter (BKTL), lactose and protein

Inhibition Activity of Garlic (Allium sativum) Skin Aqueous Extract on Mastitis Causing Microorganisms

The study was aimed to identify the effectiveness of the inhibitory activity of garlic skin extract (GSE) with different concentrations on the growth of Staphylococcus aureus, Streptococcus mutants, Escherichia coli, and Candida albicans. The study used the Kirby-Bauer method in a completely randomized (CRD) design with five treatments (positive control, negative control, 5% GSE, 10% GSE, and 15% GSE) and three replicates. The extract was obtained through evaporation of garlic skin macerated with aquadest solvent. The data were subjected to ANOVA, continued with an Honestly Significant Difference (HSD) test. The results showed that GSE concentration (minimum 5-10%) was highly significant to inhibit the growth of mastitis-causing microorganisms

Study of Local Herb Potency as Rumen Modifier: Red Ginger (Zingiber Officinale Var. Rubrum) Addition Effect on In Vitro Ruminal Nutrient Digestibility

Red ginger is herb that commonly used as part of traditional medicine due to its essential oil content. Some essential oil have proven as rumen fermentation modifier. Addition of red ginger in ruminant diet was studied using in vitro gas production technique to evaluate its effect on nutrient digestibility. Red ginger meal was added to meet essential oil level in fermentation medium of 0 mg/l as control, 25, 50, 75 and 100 mg/l. The diet consisted of Penisetum hybride, rice bran, wheat pollard in ratio 60:20:20 DM basis. Feed fermentation was incubated for 24 h at 39°C. At the end of incubation data of gas production volume was taken, and residual feed were collected for further nutrient analysis to calculate the digestibility of dry matter (DM), organic matter (OM), crude protein (CP) and crude fiber (CF). Variance analysis was used to analysis collected data, followed by DMRT analysis.  Addition of red ginger increase total volume of gas production at level 50 mg/L and above. DM and OM digestibility did not affected by red ginger addition whereas CP digestibility were significantly decreased start at level 50 mg/l. In contrast, CF digestibility of treatment level of 50 mg/l significantly higher than control. In conclusion, addition of red ginger correspond to level essential oil of 50 to 100 mg/l improve ruminal nutrient fermentation

Effect of Chloramphenicol on Rabbit Doe Fertility and Prenatal Development

Chloramphenicol (CP) is a synthetic antibiotic with large spectrum, mostly used in human and veterinary medicine to fight against diverse infections. In the present study the effects of this antibiotic on some does reproductive parameters were evaluated on 24 nulliparous, sexually mature female (6 months). The animals were divided into 4 groups of 6 does each, comparable in terms of body weight. To each group was randomly attributed by gavage 25, 50 and 75mg of CP/kg of body weight 30 days before mating (6 females for 1 male) and during the whole gestation period. After sacrifice of the does on the 28th day post-coitum, the organs (ovaries, liver, and kidneys) and fetus were collected and were examined to detect eventual anomalies. The main results showed a non-significant increase (P>0.05) of the relative weight of the liver and kidneys and a non-significant decrease (P>0.05) of that of the ovaries with increase in the dose of CP. No abortions were registered no matter the dose of CP. Gestation index, the rate of fetal mortality and of fetal viability were not significantly affected by the dose of CP used. A decreasing (P0.05) of the average weight of the fetus and their body measurement, the number of corpus luteum and the sex-ratio (M/F) were registered from treated animals in relation to the control animals. A dose-dependent increase (P<0.05) of number of pre and post-implantation resorptions were noted. The only anomaly registered was the insufficient fusion of the cranial bones at the dose 50 and 75mg/kg of CP. A significant decrease (P<0.05) of the level of proteins in the serum and ovaries were noted in animals treated in relation to those of the control group. It was concluded that CP administered at doses of 25, 50 and 75 mg/kg bw to pre and post-coital rabbit does, negatively affects fertility but has no significant teratogenic effects. Its use should therefore be limited or prohibited in husbandry

The Effect of Various Legume Herbs Supplementation on Intake, Digestibility and Growth of Fat-tailed Lambs Given Mulato Grass

This experiment was conducted to examine the effect of various legume herbs supplementation on feed intake, digestibility and liveweight gain of fat-tailed lambs given Mulato (Brachiaria mulato) grass. Twenty four male fat-tailed lambs (8 months of age and 12.73± 0.56 kg (SE) of initial liveweight) were randomly allotted to four treatment groups based on unfasted liveweight.  Animals were housed in individual metabolism crates. The experiment was designed in a completely randomized block design, with four treatments and six replicates. The dietary treatment tested included Mulato grass ad libitum (M), M + Centrosema pascuorum  (MCP), M + Dolichos lab lab (MDL) and M+ Clitoria ternatea (MCT). All supplement was offered at 1.5% body weight (W) per day.  The experiment lasted for 10 weeks, with 2 and 8 weeks for adaptation and measurement period, respectively. The main parameter measured included feed intake, feed digestibility, and average daily liveweight gain (ADG).  Results showed that total DM intakes (DMI) were not affected (P>0.05) by legume herbs supplementation. Total DMI was 2.75, 2.79, 2.84 and 2.87 %W/d for lamb treated with M MCP, MDL and MCT respectively. In contrast, supplementation with various legume herbs significantly increased (P 0.05) between legume herbs.  Feed DMD was 58.17, 67.48, 64.91; 65.03% and ADG were 35.67; 58.39; 54.31 and 54.41 g/day, for lamb treated with M, MCP, MDL and MCT respectively. It was concluded that supplementation of legume herbs to fat-tailed lamb fed Mulato grass significantly increased DMD and ADG, but no significant differences across the legume herbs as feed supplements were observed

Socio Demographic Factors Influencing the Income of Native Chicken Farming in Rural Area of Ciamis Regency

Sentul chicken is one of indigenous poultries maintained by most people in Ciamis Regency, West Java Province. This study aimed at determining the influence of socio demographic factors on the income generated from Sentul chicken farming in Ciamis Regency. A survey method was conducted to the members of seven farmer groups institutionally assisted by the Ciamis Regency Government Unit. The descriptive statistics and multiple linear regression analysis were used to describe the profile of respondents and to analyze the influence of socio demographic factors on the income generated from Sentul chicken farming. The results showed that the farmers’ length of education was 11.8 years with 5.8 years experience raising the Sentul chicken while the farm size was averagely 9 heads of chicken. The Sentul chicken farmmaintained in 70 days with semi-intensive production system has generated income ranging from IDR 756,000 to IDR 19,900,000. Farming experience and farm size significantly influenced the income generated from Sentul chicken farming. Increase the farming experience of Sentul chicken farmers and enlarging Sentul chicken farm size with semi-intensive production system may improve the income generated from Sentul chicken farming in Ciamis Regency

Development Potential of Integrated Farming System (Local Cattle - Food Crops)

Local cattle farming as an income source for farmers in rural areas is mostly developed traditionally. The local cattle farm continues, even though it is a side business, but is a mainstay in supporting national beef needs. The problem is whether integration of local cattle and food crops have the potential to be developed by farmers. The research was conducted to determine the potential integration of local cattle local and corn in North Bolaang Mongondow Regency. A survey method using a purposive sampling selected 60 farmers from Sangkub District where farmers practiced an integrated cattle-crops farming. Data were subjected to proximate and feasibility analysis. The results showed that the total cattle owned by respondents were 209. The feed consisted of grass and corn waste, with an average consumption of 5.33 and 11.15 kg/head/day, respectively. A proximate analysis of waste corn reported 86.48% dry material, 7.36% crude protein, 1.84% fat, 28.95% crude fiber, 9.10% ash and 68.18% carbohydrate.  Respondents' income from cattle farming in Bintauna and Sangkub Districts were Rp. 151,000,000 vs. Rp. 169,900,000, production costs were Rp. 101,150,625 vs. Rp. 107,298,593.8, and RC ratio was 1.49 vs. 1.58. In conclusion, corn waste consumption was greater (67.66%) than the grass. RC ratio value >1 indicated that cattle farming was feasible. The corn-cattle farming integration system can minimize environmental pollution because it enables the concept of LEISA (Low External Input Sustainability Agriculture)