Detrimental adsorbate fields in experiments with cold Rydberg gases near surfaces

We observe the shift of Rydberg levels of rubidium close to a copper surface when atomic clouds are repeatedly deposited on it. We measure transition frequencies of rubidium to S and D Rydberg states with principal quantum numbers n between 31 and 48 using the technique of electromagnetically induced transparency. The spectroscopic measurement shows a strong increase of electric fields towards the surface that evolves with the deposition of atoms. Starting with a clean surface, we measure the evolution of electrostatic fields in the range between 30 and 300 \mum from the surface. We find that after the deposition of a few hundred atomic clouds, each containing ~10^6 atoms, the field of adsorbates reaches 1 V/cm for a distance of 30 \mum from the surface. This evolution of the electrostatic field sets serious limitations on cavity QED experiments proposed for Rydberg atoms on atom chips.Comment: 4 pages, 3 figures Submitted to Phys. Rev.

Cold atoms near superconductors: Atomic spin coherence beyond the Johnson noise limit

We report on the measurement of atomic spin coherence near the surface of a superconducting niobium wire. As compared to normal conducting metal surfaces, the atomic spin coherence is maintained for time periods beyond the Johnson noise limit. The result provides experimental evidence that magnetic near field noise near the superconductor is strongly suppressed. Such long atomic spin coherence times near superconductors open the way towards the development of coherently coupled cold atom / solid state hybrid quantum systems with potential applications in quantum information processing and precision force sensing.Comment: Major revisions of the text for submission to New Journal of Physics 8 pages, 4 figure

Trapping of ultra-cold atoms with the magnetic field of vortices in a thin film superconducting micro-structure

We store and control ultra-cold atoms in a new type of trap using magnetic fields of vortices in a high temperature superconducting micro-structure. This is the first time ultra-cold atoms have been trapped in the field of magnetic flux quanta. We generate the attractive trapping potential for the atoms by combining the magnetic field of a superconductor in the remanent state with external homogeneous magnetic fields. We show the control of crucial atom trap characteristics such as an efficient intrinsic loading mechanism, spatial positioning of the trapped atoms and the vortex density in the superconductor. The measured trap characteristics are in good agreement with our numerical simulations.Comment: 4pages, comments are welcom

Cross‐scale intercomparison of climate change impacts simulated by regional and global hydrological models in eleven large river basins

Ideally, the results from models operating at different scales should agree in trend direction and magnitude of impacts under climate change. However, this implies that the sensitivity to climate variability and climate change is comparable for impact models designed for either scale. In this study, we compare hydrological changes simulated by 9 global and 9 regional hydrological models (HM) for 11 large river basins in all continents under reference and scenario conditions. The foci are on model validation runs, sensitivity of annual discharge to climate variability in the reference period, and sensitivity of the long-term average monthly seasonal dynamics to climate change. One major result is that the global models, mostly not calibrated against observations, often show a considerable bias in mean monthly discharge, whereas regional models show a better reproduction of reference conditions. However, the sensitivity of the two HM ensembles to climate variability is in general similar. The simulated climate change impacts in terms of long-term average monthly dynamics evaluated for HM ensemble medians and spreads show that the medians are to a certain extent comparable in some cases, but have distinct differences in other cases, and the spreads related to global models are mostly notably larger. Summarizing, this implies that global HMs are useful tools when looking at large-scale impacts of climate change and variability. Whenever impacts for a specific river basin or region are of interest, e.g. for complex water management applications, the regional-scale models calibrated and validated against observed discharge should be used

Near-infrared molecular imaging of tumors via chemokine receptors CXCR4 and CXCR7

The chemokine CXCL12/SDF-1 and its receptors CXCR4 and CXCR7 play a major role in tumor invasion, proliferation and metastasis. Since both receptors are overexpressed on distinct tumor cells and on the tumor vasculature, we evaluated their potential as targets for detection of cancers by molecular imaging. We synthesized conjugates of CXCL12 and the near-infrared (NIR) fluorescent dye IRDye®800CW, tested their selectivity, sensitivity and biological activity in vitro and their feasibility to visualize tumors in vivo. Purified CXCL12-conjugates detected in vitro as low as 500 A764 human glioma cells or MCF-7 breast cancer cells that express CXCR7 alone or together with CXCR4. Binding was time- and concentration-dependent, and the label could be competitively displaced by the native peptide. Control conjugates with bovine serum albumin or lactalbumin failed to label the cells. In mice, the conjugate distributed rapidly. After 1–92 h, subcutaneous tumors of human MCF-7 and A764 cells in immunodeficient mice were detected with high sensitivity. Background was observed in particular in liver within the first 24 h, but also skull and hind limbs yielded some background. Overall, fluorescent CXCL12-conjugates are sensitive and selective probes to detect solid and metastatic tumors by targeting tumor cells and tumor vasculature

One simulation, different conclusions—the baseline period makes the difference!

The choice of the baseline period, intentionally chosen or not, as a reference for assessing future changes of any projected variable can play an important role for the resulting statement. In regional climate impact studies, well-established or arbitrarily chosen baselines are often used without being questioned. Here we investigated the effects of different baseline periods on the interpretation of discharge simulations from eight river basins in the period 1960–2099. The simulations were forced by four bias-adjusted and downscaled Global Climate Modelsunder two radiative forcing scenarios (RCP 2.6 and RCP 8.5). To systematically evaluate how far the choice of different baselines impacts the simulation results, we developed a similarity index that compares two time series of projected changes. The results show that 25% of the analyzed simulations are sensitive to the choice of the baseline period under RCP 2.6 and 32% under RCP 8.5. In extreme cases, change signals of two time series show opposite trends. This has serious consequences for key messages drawn from a basin-scale climate impact study. To address this problem, an algorithm was developed to identify flexible baseline periods for each simulation individually, which better represent the statistical properties of a given historical period

RNAseq Analyses Identify Tumor Necrosis Factor-Mediated Inflammation as a Major Abnormality in ALS Spinal Cord

ALS is a rapidly progressive, devastating neurodegenerative illness of adults that produces disabling weakness and spasticity arising from death of lower and upper motor neurons. No meaningful therapies exist to slow ALS progression, and molecular insights into pathogenesis and progression are sorely needed. In that context, we used high-depth, next generation RNA sequencing (RNAseq, Illumina) to define gene network abnormalities in RNA samples depleted of rRNA and isolated from cervical spinal cord sections of 7 ALS and 8 CTL samples. We aligned \u3e50 million 2X150 bp paired-end sequences/sample to the hg19 human genome and applied three different algorithms (Cuffdiff2, DEseq2, EdgeR) for identification of differentially expressed genes (DEG’s). Ingenuity Pathways Analysis (IPA) and Weighted Gene Co-expression Network Analysis (WGCNA) identified inflammatory processes as significantly elevated in our ALS samples, with tumor necrosis factor (TNF) found to be a major pathway regulator (IPA) and TNFα-induced protein 2 (TNFAIP2) as a major network “hub” gene (WGCNA). Using the oPOSSUM algorithm, we analyzed transcription factors (TF) controlling expression of the nine DEG/hub genes in the ALS samples and identified TF’s involved in inflammation (NFkB, REL, NFkB1) and macrophage function (NR1H2::RXRA heterodimer). Transient expression in human iPSC-derived motor neurons of TNFAIP2 (also a DEG identified by all three algorithms) reduced cell viability and induced caspase 3/7 activation. Using high-density RNAseq, multiple algorithms for DEG identification, and an unsupervised gene co-expression network approach, we identified significant elevation of inflammatory processes in ALS spinal cord with TNF as a major regulatory molecule. Overexpression of the DEG TNFAIP2 in human motor neurons, the population most vulnerable to die in ALS, increased cell death and caspase 3/7 activation. We propose that therapies targeted to reduce inflammatory TNFα signaling may be helpful in ALS patients

Evidence for a grounding line fan at the onset of a basal channel under the ice shelf of Support Force Glacier, Antarctica, revealed by reflection seismics

Curvilinear channels on the surface of an ice shelf indicate the presence of large channels at the base. Modelling studies have shown that where these surface expressions intersect the grounding line, they coincide with the likely outflow of subglacial water. An understanding of the initiation and the ice–ocean evolution of the basal channels is required to understand the present behaviour and future dynamics of ice sheets and ice shelves. Here, we present focused active seismic and radar surveys of a basal channel, ∼950 m wide and ∼200 m high, and its upstream continuation beneath Support Force Glacier, which feeds into the Filchner Ice Shelf, West Antarctica. Immediately seaward from the grounding line, below the basal channel, the seismic profiles show an ∼6.75 km long, 3.2 km wide and 200 m thick sedimentary sequence with chaotic to weakly stratified reflections we interpret as a grounding line fan deposited by a subglacial drainage channel directly upstream of the basal channel. Further downstream the seabed has a different character; it consists of harder, stratified consolidated sediments, deposited under different glaciological circumstances, or possibly bedrock. In contrast to the standard perception of a rapid change in ice shelf thickness just downstream of the grounding line, we find a flat topography of the ice shelf base with an almost constant ice thickness gradient along-flow, indicating only little basal melting, but an initial widening of the basal channel, which we ascribe to melting along its flanks. Our findings provide a detailed view of a more complex interaction between the ocean and subglacial hydrology to form basal channels in ice shelves