114 research outputs found
Overlapping resonances in the control of intramolecular vibrational redistribution
Coherent control of bound state processes via the interfering overlapping
resonances scenario [Christopher et al., J. Chem. Phys. 123, 064313 (2006)] is
developed to control intramolecular vibrational redistribution (IVR). The
approach is applied to the flow of population between bonds in a model of
chaotic OCS vibrational dynamics, showing the ability to significantly alter
the extent and rate of IVR by varying quantum interference contributions.Comment: 10 pages, 7 figure
Blanks, a nuclear siRNA/dsRNA-binding complex component, is required for Drosophila spermiogenesis
Small RNAs and a diverse array of protein partners control gene expression in eukaryotes through a variety of mechanisms. By combining siRNA affinity chromatography and mass spectrometry, we have identified the double-stranded RNA-binding domain protein Blanks to be an siRNA- and dsRNA-binding protein from Drosophila S2 cells. We find that Blanks is a nuclear factor that contributes to the efficiency of RNAi. Biochemical fractionation of a Blanks-containing complex shows that the Blanks complex is unlike previously described RNA-induced silencing complexes and associates with the DEAD-box helicase RM62, a protein previously implicated in RNA silencing. In flies, Blanks is highly expressed in testes tissues and is necessary for postmeiotic spermiogenesis, but loss of Blanks is not accompanied by detectable transposon derepression. Instead, genes related to innate immunity pathways are up-regulated in blanks mutant testes. These results reveal Blanks to be a unique component of a nuclear siRNA/dsRNA-binding complex that contributes to essential RNA silencing-related pathways in the male germ line
Functional Leadership in Interteam Contexts: Understanding ‘What’ in the Context of Why? Where? When? and Who?
This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this recordResearch on team leadership has primarily focused on leadership processes targeted within
teams, in support of team objectives. Yet, teams are open systems that interact with other teams
to achieve proximal as well as distal goals. This review clarifies that defining ‘what’ constitutes
functionally effective leadership in interteam contexts requires greater precision with regard to
where (within teams, across teams) and why (team goals, system goals) leadership processes are
enacted, as well as greater consideration of when and among whom leadership processes arise.
We begin by synthesizing findings from empirical studies published over the past 30 years that
shed light on questions of what, where, why, when, and who related to interteam leadership and
end by providing three overarching recommendations for how research should proceed in order
to provide a more comprehensive picture of leadership in interteam contexts
The Lyman Continuum escape fraction of galaxies at z=3.3 in the VUDS-LBC/COSMOS field
The Lyman continuum (LyC) flux escaping from high-z galaxies into the IGM is
a fundamental quantity to understand the physical processes involved in the
reionization epoch. We have investigated a sample of star-forming galaxies at
z~3.3 in order to search for possible detections of LyC photons escaping from
galaxy halos. UV deep imaging in the COSMOS field obtained with the prime focus
camera LBC at the LBT telescope was used together with a catalog of
spectroscopic redshifts obtained by the VIMOS Ultra Deep Survey (VUDS) to build
a sample of 45 galaxies at z~3.3 with L>0.5L*. We obtained deep LBC images of
galaxies with spectroscopic redshifts in the interval 3.27<z<3.40 both in the R
and deep U bands. A sub-sample of 10 galaxies apparently shows escape
fractions>28% but a detailed analysis of their properties reveals that, with
the exception of two marginal detections (S/N~2) in the U band, all the other 8
galaxies are most likely contaminated by the UV flux of low-z interlopers
located close to the high-z targets. The average escape fraction derived from
the stacking of the cleaned sample was constrained to fesc_rel<2%. The implied
HI photo-ionization rate is a factor two lower than that needed to keep the IGM
ionized at z~3, as observed in the Lyman forest of high-z QSO spectra or by the
proximity effect. These results support a scenario where high redshift,
relatively bright (L>0.5L*) star-forming galaxies alone are unable to sustain
the level of ionization observed in the cosmic IGM at z~3. Star-forming
galaxies at higher redshift and at fainter luminosities (L<<L*) can be the
major contributors to the reionization of the Universe only if their physical
properties are subject to rapid changes from z~3 to z~6-10. Alternatively,
ionizing sources could be discovered looking for fainter sources among the AGN
population at high-z.Comment: 21 pages, 9 figures. Accepted for publication in A&
Asc1 Supports Cell-Wall Integrity Near Bud Sites by a Pkc1 Independent Mechanism
Background: The yeast ribosomal protein Asc1 is a WD-protein family member. Its mammalian ortholog, RACK1 was initially discovered as a receptor for activated protein C kinase (PKC) that functions to maintain the active conformation of PKC and to support its movement to target sites. In the budding yeast though, a connection between Asc1p and the PKC signaling pathway has never been reported. Methodology/Principal Findings: In the present study we found that asc1-deletion mutant (asc1D) presents some of the hallmarks of PKC signaling mutants. These include an increased sensitivity to staurosporine, a specific Pkc1p inhibitor, and susceptibility to cell-wall perturbing treatments such as hypotonic- and heat shock conditions and zymolase treatment. Microscopic analysis of asc1D cells revealed cell-wall invaginations near bud sites after exposure to hypotonic conditions, and the dynamic of cells ’ survival after this stress further supports the involvement of Asc1p in maintaining the cell-wall integrity during the mid-to late stages of bud formation. Genetic interactions between asc1 and pkc1 reveal synergistic sensitivities of a double-knock out mutant (asc1D/pkc1D) to cell-wall stress conditions, and high basal level of PKC signaling in asc1D. Furthermore, Asc1p has no effect on the cellular distribution or redistribution of Pkc1p at optimal or at cell-wall stress conditions. Conclusions/Significance: Taken together, our data support the idea that unlike its mammalian orthologs, Asc1p act
Trypanosomatid RACK1 Orthologs Show Functional Differences Associated with Translation Despite Similar Roles in Leishmania Pathogenesis
RACK1 proteins belong to the eukaryote WD40-repeat protein family and function as spatial regulators of multiple cellular events, including signaling pathways, the cell cycle and translation. For this latter role, structural and genetic studies indicate that RACK1 associates with the ribosome through two conserved positively charged amino acids in its first WD40 domain. Unlike RACK1s, including Trypanosoma brucei RACK1 (TbRACK1), only one of these two positively-charged residues is conserved in the first WD40 domain of the Leishmania major RACK1 ortholog, LACK. We compared virulence-attenuated LACK single copy (LACK/-) L. major, with L. major expressing either two LACK copies (LACK/LACK), or one copy each of LACK and TbRACK1 (LACK/TbRACK1), to evaluate the function of these structurally distinct RACK1 orthologs with respect to translation, viability at host temperatures and pathogenesis. Our results indicate that although the ribosome-binding residues are not fully conserved in LACK, both LACK and TbRACK1 co-sedimented with monosomes and polysomes in LACK/LACK and LACK/TbRACK1 L. major, respectively. LACK/LACK and LACK/TbRACK1 strains differed in their sensitivity to translation inhibitors implying that minor sequence differences between the RACK1 proteins can alter their functional properties. While biochemically distinguishable, both LACK/LACK and LACK/TbRACK1 lines were more tolerant of elevated temperatures, resistant to translation inhibitors, and displayed robust pathogenesis in vivo, contrasting to LACK/- parasites
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Mental health training for secondary school teachers in Haiti: a mixed methods, prospective, formative research study of feasibility, acceptability, and effectiveness in knowledge acquisition
Background: Engagement and training of educators in student mental health holds promise for promoting access to care as a task sharing strategy but has not been well-studied in low-income regions. Methods: We used a prospective and convergent mixed methods design to evaluate a customized school mental health 2½ day training for teachers in rural Haiti (n = 22) as the initial component of formative research developing a school-based intervention to promote student mental health. Training prepared teachers to respond to student mental health needs by providing psychoeducational and practical support to facilitate access to care. We examined level of participation and evaluated feasibility, acceptability, and perceived effectiveness by calculating mean scores on self-report Likert-style items eliciting participant experience. We examined effectiveness of the training on improving mental health knowledge and attitudes by comparing mean scores on an assessment administered pre- and post-training. Finally, we examined self-report written open-ended responses and focus group discussion (FGD) interview data bearing on perceived feasibility, acceptability, and effectiveness to contextualize participant ratings of training and to identify recommendations for enhancing the utility of mental health training locally for educators. Results: Mean scores of knowledge and attitudes significantly improved between the pre-test and post-tests; e.g., knowledge improved from 58% correct at baseline to 68% correct on the second post-test (p = 0.039). Mean ratings of the training were favorable across all categories and FGD data demonstrated widespread participant endorsement of training acceptability and effectiveness; participants recommended extending the duration and number of training sessions. Conclusions: Findings support feasibility, acceptability, and a limited scope of effectiveness of brief mental health training for secondary school teachers in Haiti. Further development of approaches to engage teachers in promoting school mental health through training is warranted
Rational Extension of the Ribosome Biogenesis Pathway Using Network-Guided Genetics
Gene networks are an efficient route for associating candidate genes with biological processes. Here, networks are used to discover more than 15 new genes for ribosomal subunit maturation, rRNA processing, and ribosomal export from the nucleus
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