11,266 research outputs found

    \u3ci\u3eAcrobasis\u3c/i\u3e Shoot Moth (Lepidoptera: Pyralidae) Infestation-Tree Height Link in a Young Black Walnut Plantation

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    Acrobasis shoot moth infestations were evaluated in a young black walnut progeny test for 4 years, from ages 3 to 6. Infestation levels were greatest on the largest trees in the fourth and fifth year after plantation establishment, and were declining by the sixth year. Acrobasis infestation appears to be a problem primarily on young trees less than 2.5 m in height. There was no evidence for genetic resistance to Acrobasis infestation in black walnut

    iREAD: a tool for intron retention detection from RNA-seq data.

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    BACKGROUND: Intron retention (IR) has been traditionally overlooked as \u27noise\u27 and received negligible attention in the field of gene expression analysis. In recent years, IR has become an emerging field for interrogating transcriptomes because it has been recognized to carry out important biological functions such as gene expression regulation and it has been found to be associated with complex diseases such as cancers. However, methods for detecting IR today are limited. Thus, there is a need to develop novel methods to improve IR detection. RESULTS: Here we present iREAD (intron REtention Analysis and Detector), a tool to detect IR events genome-wide from high-throughput RNA-seq data. The command line interface for iREAD is implemented in Python. iREAD takes as input a BAM file, representing the transcriptome, and a text file containing the intron coordinates of a genome. It then 1) counts all reads that overlap intron regions, 2) detects IR events by analyzing the features of reads such as depth and distribution patterns, and 3) outputs a list of retained introns into a tab-delimited text file. iREAD provides significant added value in detecting IR compared with output from IRFinder with a higher AUC on all datasets tested. Both methods showed low false positive rates and high false negative rates in different regimes, indicating that use together is generally beneficial. The output from iREAD can be directly used for further exploratory analysis such as differential intron expression and functional enrichment. The software is freely available at https://github.com/genemine/iread. CONCLUSION: Being complementary to existing tools, iREAD provides a new and generic tool to interrogate poly-A enriched transcriptomic data of intron regions. Intron retention analysis provides a complementary approach for understanding transcriptome

    Peology and Geochemistry of New Paired Martian Meteorites 12095 and LAR 12240

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    The meteorites LAR 12095 and LAR 12240 are believed to be paired Martian meteorites and were discovered during the Antarctic Search for Meteorites (ANSMET) 2012-2013 Season at Larkman Nunatak. The purpose of this study is to characterize these olivine-phyric shergottites by analyzing all mineral phases for major, minor and trace elements and examining their textural relationships. The goal is to constrain their crystallization history and place these shergottites among other Martian meteorites in order to better understand Martian geological history

    I Am Distinctive When I Belong: Meeting the Need for Optimal Distinctiveness through Team Identification

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    The development of stronger team identity has previously been explained through the social identity aspect of belonging. Although this has contributed much to our understanding of sport fans, it has neglected an alternative explanation for team identity, specifically the search for distinctiveness. How then do fans develop stronger team identity by \u27standing out\u27 as opposed to \u27fitting in\u27? This paper provides evidence of seven identity management strategies used by fans with a strong psychological connection to their chosen team. Saturation sampling was employed to interview 29 South African rugby union fans via semi-structured interviews, followed by a directed approach to content analysis. The results contribute a stronger explanation of how the psychological need for optimal distinctiveness functions within the attachment process towards stronger fan loyalty, and provides a more complete explanation for the way in which fans can \u27stand out\u27 while still belonging

    Dark matter annihilation and decay in dwarf spheroidal galaxies: The classical and ultrafaint dSphs

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    Dwarf spheroidal (dSph) galaxies are prime targets for present and future gamma-ray telescopes hunting for indirect signals of particle dark matter. The interpretation of the data requires careful assessment of their dark matter content in order to derive robust constraints on candidate relic particles. Here, we use an optimised spherical Jeans analysis to reconstruct the `astrophysical factor' for both annihilating and decaying dark matter in 21 known dSphs. Improvements with respect to previous works are: (i) the use of more flexible luminosity and anisotropy profiles to minimise biases, (ii) the use of weak priors tailored on extensive sets of contamination-free mock data to improve the confidence intervals, (iii) systematic cross-checks of binned and unbinned analyses on mock and real data, and (iv) the use of mock data including stellar contamination to test the impact on reconstructed signals. Our analysis provides updated values for the dark matter content of 8 `classical' and 13 `ultrafaint' dSphs, with the quoted uncertainties directly linked to the sample size; the more flexible parametrisation we use results in changes compared to previous calculations. This translates into our ranking of potentially-brightest and most robust targets---viz., Ursa Minor, Draco, Sculptor---, and of the more promising, but uncertain targets---viz., Ursa Major 2, Coma---for annihilating dark matter. Our analysis of Segue 1 is extremely sensitive to whether we include or exclude a few marginal member stars, making this target one of the most uncertain. Our analysis illustrates challenges that will need to be addressed when inferring the dark matter content of new `ultrafaint' satellites that are beginning to be discovered in southern sky surveys.Comment: 19 pages, 14 figures, submitted to MNRAS. Supplementary material available on reques

    Screening for childhood anaemia using copper sulphate densitometry

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    Objective. To evaluate copper sulphate densitometry to screen for childhood anaemia in a primary care setting, with a view to identifying children requiring definitive diagnostic testing and treatment. Design. A cross-sectional screening study. Results of densitometry with a copper sulphate solution of specific gravity (SG) 1.048, corresponding to a haemoglobin (Hb) concentration of 10 g/dl, were compared with laboratory Hb determination. Setting. Outpatient department of Pretoria Academic Hospital (73 children) and a local cr_che (27 children). Subjects. One hundred consecutive children, aged between 6 months and 6 years, with informed written consent by parents. Outcome measure(s). Accuracy of copper sulphate densitometry in screening for Hb concentration below 10 g/dl in terms of sensitivity, specificity, positive and negative predictive values, as well as likelihood ratio. Results. The prevalence of anaemia (Hb < 10 g/dl) was 17% (95% confidence interval (CI) 10.2; 25.8). Copper sulphate densitometry had a sensitivity of 88.2% (95% CI 62.3; 97.9), a specificity of 89.2% (95% CI 79.9; 94.6), a positive predictive value of 62.5% (95% CI 40.8; 80.5) and a negative predictive value of 97.4% (95%CI 90.0; 99.5) in screening for anaemia. The likelihood ratio of a positive screening test was 8.17. Conclusions. Copper sulphate densitometry was accurate in screening for childhood anaemia. (South African Medical Journal: 2002 92(12): 978-981

    Desorption of CO from Ru(001) induced by near-infrared femtosecond laser pulses

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    Irradiation of a Ru(001) surface covered with CO using intense femtosecond laser pulses (800 nm, 130 fs) leads to desorption of CO with a nonlinear dependence of the yield on the absorbed fluence (100–380 J/m2). Two-pulse correlation measurements reveal a response time of 20 ps (FWHM). The lack of an isotope effect together with the strong rise of the phonon temperature (2500 K) and the specific electronic structure of the adsorbate–substrate system strongly indicate that coupling to phonons is dominant. The experimental findings can be well reproduced within a friction-coupled heat bath model. Yet, pronounced dynamical cooling in desorption, found in the fluence-dependence of the translational energy, and in a non-Arrhenius behavior of the desorption probability reflect pronounced deviations from thermal equilibrium during desorption taking place on such a short time scale

    Accuracy of Wrist-worn Physical Activity Monitors to Measure Energy Expenditure

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    IIn recent years, the popularity and demand of physical activity monitors has drastically risen with the need and want to improve physical fitness. Newer devices worn on the wrist measure both heart rate and energy expenditure but the accuracy of these measurements is unclear. PURPOSE: To measure the accuracy of three separate wrist-worn activity monitors to estimate energy expenditure during structured periods of aerobic exercise. METHODS: Twelve men and three women (22 ± 3 years, 25 ± 3 kg/m2) consented to participate in this study. Three different physical activity monitors, TomTom Cardio (TT), Microsoft Band (MB), and Fitbit Surge (FB), were randomly assigned to either the left or right wrist of each participant. The instructions for the testing procedure were thoroughly explained to every participant at the start of each trial. The treadmill started at a speed of 2 mph and increased by 1 mph every three minutes up to a max speed of 6 mph. Energy expenditure was estimated through direct measurement of oxygen consumed and carbon dioxide produced through a metabolic cart (MC, Parvo Medics True One ®2400). The mean bias in energy expenditure between MC and each device was calculated. Pearson product-moment correlations and 95% equivalence testing were also calculated. Statistical significance was set at an alpha level of 0.05. RESULTS: The mean bias between the MC and devices at 2 mph varied from -1.9 ± 1.1 kcal/min (FB) to 0.7 ± 1.0 kcal/min (MB) while the mean bias at 6 mph varied from -1.7 ± 2.1 kcal/min (MB) to 5.2 ± 1.7 kcal/min (TT). For total energy expenditure, all devices were significantly correlated with the MC (FB: r=0.66, p=0.007; TomTom: r=0.77, p\u3c0.001; MB: r=0.59, p=0.02). The mean bias for total energy expenditure was -25 ± 16 kcal for the FB, 26 ± 13 kcal for the TT, and -11 ± 17 kcal for the MB. The equivalence zone for MC was 88 kcal to 108 kcal but 90% confidence intervals of devices did not fall within this zone. CONCLUSION: The wrist-worn physical activity monitors used in this study that measure heart rate and energy expenditure tend to either underestimate or overestimate total energy expenditure from treadmill walking and running

    Quantum key distribution using non-classical photon number correlations in macroscopic light pulses

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    We propose a new scheme for quantum key distribution using macroscopic non-classical pulses of light having of the order 10^6 photons per pulse. Sub-shot-noise quantum correlation between the two polarization modes in a pulse gives the necessary sensitivity to eavesdropping that ensures the security of the protocol. We consider pulses of two-mode squeezed light generated by a type-II seeded parametric amplification process. We analyze the security of the system in terms of the effect of an eavesdropper on the bit error rates for the legitimate parties in the key distribution system. We also consider the effects of imperfect detectors and lossy channels on the security of the scheme.Comment: Modifications:added new eavesdropping attack, added more references Submitted to Physical Review A [email protected]
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