Visibility graphs and landscape visibility analysis

Visibility analysis based on viewsheds is one of the most frequently used GIS analysis tools. In this paper we present an approach to visibility analysis based on the visibility graph. A visibility graph records the pattern of mutual visibility relations in a landscape, and provides a convenient way of storing and further analysing the results of multiple viewshed analyses for a particular landscape region. We describe how a visibility graph may be calculated for a landscape. We then give examples, which include the interactive exploration ofa landscape, and the calculation of new measures of a landscape?s visual properties based on graph metrics ? in particular, neighbourhood clustering coefficient and path length analysis. These analyses suggest that measures derived from the visibility graph may be of particular relevance to the growing interest in quantifying the perceptual characteristics of landscapes

From isovists to visibility graphs: a methodology for the analysis of architectural space

An isovist, or viewshed, is the area in a spatial environment directly visible from a location within the space. Here we show how a set of isovists can be used to generate a graph of mutual visibility between locations. We demonstrate that this graph can also be constructed without reference to isovists and that we are in fact invoking the more general concept of a visibility graph. Using the visibility graph, we can extend both isovist and current graph-based analyses of architectural space to form a new methodology for the investigation of configurational relationships. The measurement of local and global characteristics of the graph, for each vertex or for the system as a whole, is of interest from an architectural perspective, allowing us to describe a configuration with reference to accessibility and visibility, to compare from location to location within a system, and to compare systems with different geometries. Finally we show that visibility graph properties may be closely related to manifestations of spatial perception, such as way-finding, movement, and space use

Canine dystocia in 50 UK first-opinion emergency-care veterinary practices: prevalence and risk factors

Dystocia can represent a major welfare issue for dogs of certain breeds and morphologies. First-opinion emergency-care veterinary caseloads represent a useful data resource for epidemiological research because dystocia can often result in emergency veterinary care. The study analysed a merged database of clinical records from 50 first-opinion emergency-care veterinary practices participating in the VetCompass Programme. Multivariable logistic regression modelling was used for risk factors analysis. There were 701 dystocia cases recorded among 18,758 entire female dogs, resulting in a dystocia prevalence of 3.7 per cent (95 per cent CI 3.5–4.0 per cent). Breeds with the highest odds of dystocia compared with crossbred bitches were French Bulldog (OR: 15.9, 95 per cent CI 9.3 to 27.2, P<0.001), Boston Terrier (OR: 12.9, 95 per cent CI 5.6 to 29.3, P<0.001), Chihuahua (OR: 10.4, 95 per cent CI 7.0 to 15.7, P<0.001) and Pug (OR: 11.3, 95 per cent CI 7.1 to 17.9, P<0.001). Bitches aged between 3.0 and 5.9 years had 3.1 (95 per cent CI 2.6 to 3.7, P<0.001) times the odds of dystocia compared with bitches aged under 3.0years. Certain breeds, including some brachycephalic and toy breeds, appeared at high risk of dystocia. Opportunities to improve this situation are discussed

Increasing concentrations of dichloromethane, CH2Cl2, inferred from CARIBIC air samples collected 1998–2012

Atmospheric concentrations of dichloromethane, CH2Cl2, a regulated toxic air pollutant and minor contributor to stratospheric ozone depletion, were reported to have peaked around 1990 and to be declining in the early part of the 21st century. Recent observations suggest this trend has reversed and that CH2Cl2 is once again increasing in the atmosphere. Despite the importance of ongoing monitoring and reporting of atmospheric CH2Cl2, no time series has been discussed in detail since 2006. The CARIBIC project (Civil Aircraft for the Regular Investigation of the atmosphere Based on an Instrument Container) has analysed the halocarbon content of whole-air samples collected at altitudes of between ~10–12 km via a custom-built container installed on commercial passenger aircraft since 1998, providing a long-term record of CH2Cl2 observations. In this paper we present this unique CH2Cl2 time series, discussing key flight routes which have been used at various times over the past 15 years. Between 1998 and 2012 increases were seen in all northern hemispheric regions and at different altitudes, ranging from ~7–10 ppt in background air to ~13–15 ppt in regions with stronger emissions (equating to a 38–69% increase). Of particular interest is the rising importance of India as a source of atmospheric CH2Cl2: based on CARIBIC data we provide regional emission estimates for the Indian subcontinent and show that regional emissions have increased from 3–14 Gg yr^-1 (1998–2000) to 16–25 Gg yr^-1 (2008). Potential causes of the increasing atmospheric burden of CH2Cl2 are discussed. One possible source is the increased use of CH2Cl2 as a feedstock for the production of HFC-32, a chemical used predominantly as a replacement for ozone-depleting substances in a variety of applications including air conditioners and refrigeration

Constriction size distributions of granular filters: a numerical study

The retention capability of granular filters is controlled by the narrow constrictions connecting the voids within the filter. The theoretical justification for empirical filter rules used in practice includes consideration of an idealised soil fabric in which constrictions form between co-planar combinations of spherical filter particles. This idealised fabric has not been confirmed by experimental or numerical observations of real constrictions. This paper reports the results of direct, particle-scale measurement of the constriction size distribution (CSD) within virtual samples of granular filters created using the discrete-element method (DEM). A previously proposed analytical method that predicts the full CSD using inscribed circles to estimate constriction sizes is found to poorly predict the CSD for widely graded filters due to an over-idealisation of the soil fabric. The DEM data generated are used to explore quantitatively the influence of the coefficient of uniformity, particle size distribution and relative density of the filter on the CSD. For a given relative density CSDs form a narrow band of similarly shaped curves when normalised by characteristic filter diameters. This lends support to the practical use of characteristic diameters to assess filter retention capability

The X-ray Emission in Post-Merger Ellipticals

The evolution in X-ray properties of early-type galaxies is largely unconstrained. In particular, little is known about how, and if, remnants of mergers generate hot gas halos. Here we examine the relationship between X-ray luminosity and galaxy age for a sample of early-type galaxies. Comparing normalized X-ray luminosity to three different age indicators we find that L_X/L_B increases with age, suggesting an increase in X-ray halo mass with time after a galaxy's last major star-formation episode. The long-term nature of this trend, which appears to continue across the full age range of our sample, poses a challenge for many models of hot halo formation. We conclude that models involving a declining rate of type Ia supernovae, and a transition from outflow to inflow of the gas originally lost by galactic stars, offers the most promising explanation for the observed evolution in X-ray luminosity

X-ray observations of three young, early-type galaxies

Massive haloes of hot plasma exist around some, but not all elliptical galaxies. There is evidence that this is related to the age of the galaxy. In this paper, new X-ray observations are presented for three early-type galaxies that show evidence of youth, in order to investigate their X-ray components and properties. NGC 5363 and NGC 2865 were found to have X-ray emission dominated by purely discrete stellar sources. Limits are set on the mass distribution in one of the galaxies observed with XMM–Newton, NGC 4382, which contains significant hot gas. We detect the X-ray emission in NGC 4382 out to 4re. The mass-to-light ratio is consistent with a stellar origin in the inner regions but rises steadily to values indicative of some dark matter by 4re. These results are set in context with other data drawn from the literature, for galaxies with ages estimated from dynamical or spectroscopic indicators. Ages obtained from optical spectroscopy represent central luminosity-weighted stellar ages. We examine the X-ray evolution with age, normalized by B- and K-band luminosities. Low values of Log(LX/LB) and Log(LX/LK) are found for all galaxies with ages between 1 and 4 Gyr. Luminous X-ray emission only appears in older galaxies. This suggests that the interstellar medium is removed and then it takes several gigayears for hot gas haloes to build up, following a merger. A possible mechanism for gas expulsion might be associated with feedback from an active nucleus triggered during a merger

Enhanced heterogeneity of rpoB in Mycobacterium tuberculosis found at low pH.

OBJECTIVES: The aim of this study was to gain an insight into the molecular mechanisms of the evolution of rifampicin resistance in response to controlled changes in the environment. METHODS: We determined the proportion of rpoB mutants in the chemostat culture and characterized the sequence of mutations found in the rifampicin resistance-determining region of rpoB in a steady-state chemostat at pH 7.0 and 6.2. RESULTS: The overall proportion of rpoB mutants of strain H37Rv remained constant for 37 days at pH 7.0, ranging between 3.6 x 10(-8) and 8.9 x 10(-8); however, the spectrum of mutations varied. The most commonly detected mutation, serine to leucine mutation at codon 531 (S531L), increased from 40% to 89%, while other mutations (S531W, H526Y, H526D, H526R, S522L and D516V) decreased over the 37 day sampling period. Changing the pH from 7.0 to 6.2 did not significantly alter the overall proportion of mutants, but resulted in a decrease in the percentage of strains harbouring S531L (from 89% to 50%) accompanied by an increase in the range of different mutations from 4 to 12. CONCLUSIONS: The data confirm that the fitness of strains with the S531L mutation is greater than that of strains containing other mutations. We also conclude that at low pH the environment is permissive for a wider spectrum of mutations, which may provide opportunities for a successful mutant to survive

Widespread dysregulation of MiRNAs by MYCN amplification and chromosomal imbalances in neuroblastoma: association of miRNA expression with survival

MiRNAs regulate gene expression at a post-transcriptional level and their dysregulation can play major roles in the pathogenesis of many different forms of cancer, including neuroblastoma, an often fatal paediatric cancer originating from precursor cells of the sympathetic nervous system. We have analyzed a set of neuroblastoma (n = 145) that is broadly representative of the genetic subtypes of this disease for miRNA expression (430 loci by stem-loop RT qPCR) and for DNA copy number alterations (array CGH) to assess miRNA involvement in disease pathogenesis. The tumors were stratified and then randomly split into a training set (n = 96) and a validation set (n = 49) for data analysis. Thirty-seven miRNAs were significantly over-or under-expressed in MYCN amplified tumors relative to MYCN single copy tumors, indicating a potential role for the MYCN transcription factor in either the direct or indirect dysregulation of these loci. In addition, we also determined that there was a highly significant correlation between miRNA expression levels and DNA copy number, indicating a role for large-scale genomic imbalances in the dysregulation of miRNA expression. In order to directly assess whether miRNA expression was predictive of clinical outcome, we used the Random Forest classifier to identify miRNAs that were most significantly associated with poor overall patient survival and developed a 15 miRNA signature that was predictive of overall survival with 72.7% sensitivity and 86.5% specificity in the validation set of tumors. We conclude that there is widespread dysregulation of miRNA expression in neuroblastoma tumors caused by both over-expression of the MYCN transcription factor and by large-scale chromosomal imbalances. MiRNA expression patterns are also predicative of clinical outcome, highlighting the potential for miRNA mediated diagnostics and therapeutics